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Q6UJY2

- SL9C1_MOUSE

UniProt

Q6UJY2 - SL9C1_MOUSE

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Protein

Sodium/hydrogen exchanger 10

Gene

Slc9c1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Sperm-specific sodium/hydrogen exchanger involved in intracellular pH regulation of spermatozoa. Required for sperm motility and fertility. Involved in sperm cell hyperactivation, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for the expression and bicarbonate regulation of the soluble adenylyl cyclase (sAC).2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi890 – 1026137cNMPAdd
BLAST

GO - Molecular functioni

  1. solute:proton antiporter activity Source: InterPro

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. sodium ion transport Source: UniProtKB-KW
  4. spermatogenesis Source: UniProtKB-KW
  5. sperm motility Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Antiport, Differentiation, Ion transport, Sodium transport, Spermatogenesis, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_196640. Stimuli-sensing channels.

Protein family/group databases

TCDBi2.A.36.7.7. the monovalent cation:proton antiporter-1 (cpa1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 10
Alternative name(s):
Na(+)/H(+) exchanger 10
Short name:
NHE-10
Solute carrier family 10 member 10
Solute carrier family 9 member C1
Sperm-specific Na(+)/H(+) exchanger
Short name:
sNHE
Gene namesi
Name:Slc9c1
Synonyms:Gm610, Slc9a10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:2685456. Slc9c1.

Subcellular locationi

Cell projectionciliumflagellum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei37 – 5721HelicalSequence AnalysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence AnalysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence AnalysisAdd
BLAST
Transmembranei125 – 14521HelicalSequence AnalysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence AnalysisAdd
BLAST
Transmembranei192 – 21221HelicalSequence AnalysisAdd
BLAST
Transmembranei240 – 26021HelicalSequence AnalysisAdd
BLAST
Transmembranei269 – 28921HelicalSequence AnalysisAdd
BLAST
Transmembranei314 – 33421HelicalSequence AnalysisAdd
BLAST
Transmembranei353 – 37321HelicalSequence AnalysisAdd
BLAST
Transmembranei420 – 44021HelicalSequence AnalysisAdd
BLAST
Transmembranei633 – 65321HelicalSequence AnalysisAdd
BLAST
Transmembranei666 – 68621HelicalSequence AnalysisAdd
BLAST
Transmembranei693 – 71321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. ciliary membrane Source: Reactome
  2. integral component of membrane Source: UniProtKB-KW
  3. motile cilium Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Flagellum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are normal but males are sterile. Male sterility is due to defects in sperm motility inability to fertilize intact eggs. Moreover, spermatozoa fail to develop the cAMP-dependent protein tyrosine phosphorylation that coincides with the functional maturation occurring upon incubation in capacitating conditions in vitro. cAMP analogs almost completely rescue the motility and infertility phenotypes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11751175Sodium/hydrogen exchanger 10PRO_0000295705Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ6UJY2.

PTM databases

PhosphoSiteiQ6UJY2.

Expressioni

Tissue specificityi

Testis-specific. Specifically present in the principal piece of sperm tail (at protein level).1 Publication

Gene expression databases

BgeeiQ6UJY2.
CleanExiMM_SLC9A10.
ExpressionAtlasiQ6UJY2. baseline.
GenevestigatoriQ6UJY2.

Interactioni

Subunit structurei

Interacts with soluble adenylyl cyclase (sAC).1 Publication

Protein-protein interaction databases

DIPiDIP-60951N.
STRINGi10090.ENSMUSP00000037782.

Structurei

3D structure databases

ProteinModelPortaliQ6UJY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni618 – 69881Ion transport-likeAdd
BLAST

Domaini

The ion transport-like region is related to the membrane segments of voltage-gated ion channels. Its function is unknown.

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0025.
GeneTreeiENSGT00530000063840.
HOGENOMiHOG000169764.
HOVERGENiHBG071202.
InParanoidiQ6UJY2.
KOiK14726.
OMAiASYQRQY.
OrthoDBiEOG7N37BW.
PhylomeDBiQ6UJY2.
TreeFamiTF328865.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR027359. Channel_four-helix_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR028483. N/H_exchanger_10.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF87. PTHR10110:SF87. 1 hit.
PfamiPF00027. cNMP_binding. 1 hit.
PF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6UJY2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEMEEISENL TASHSIKLTN MWLELLKSVF LSTPQDLPEI ILILSLICTV
60 70 80 90 100
GAFLNMHLKD FPIPLPVILF LIGCCFEILS FASTQIQIYA DAIQWMDPDI
110 120 130 140 150
FFGIFTPVII FNVAFDMDIY MLQKLFWQIL VITIPGFLIN YTLILWYLQS
160 170 180 190 200
VNKLSLKTVP WLLFSAVLIS SDPMLTSASI RDLGLSRSLT NLINGESLLT
210 220 230 240 250
SVLSLVIYSG VVHIRFKSKS VNHTLAHKVM STAWSYIVES FITGIVFTKV
260 270 280 290 300
IQLWMATIFG DDVNHITLIF SVLYLIFYVC ELVGMSGIFT LATIGLFLNS
310 320 330 340 350
TSFKPGVEAF LLEFWNCLSF IGFLMVFTFI GLLIPAHTYL HISFSDVYYS
360 370 380 390 400
LNIYFTLIVL RLLVFLLMSP ILSRLGHGFS WRWAFIMVWS EMKGTPNINM
410 420 430 440 450
ALLLAYSDIS LGSERERSQI LFHGVSVCVI TLIVNRFILP MAVTKLGLRD
460 470 480 490 500
VTSTKYKSVY YTFQHFQELT KSTAMALKFD KDLANADWNM VDNAIILQNP
510 520 530 540 550
YAMNQEEITE HQKVKCPDCN KEIDETLNIE AMELTNRRLL SAQIASYQRQ
560 570 580 590 600
YRNEVLSQSA VQVLVGAAGS FGEKKGEYMS PENIKNFSES KKLLSFLRKL
610 620 630 640 650
LLNWVYNTKK DKGVPSRYMF LHACHRIVFT NEFEYTGYLV VLMSTYPMII
660 670 680 690 700
CWISRLKDIY DNEIKCANYY FLAFYILEAL LKVAAMRKEF FSHTWLLFEL
710 720 730 740 750
GITLVGVLDI ILIETDSISY NFDLTETVVF MNVIRLLRIL RILKLVTPKL
760 770 780 790 800
LQIIDKRMSQ QISFRYSILK GYVQGEMDVL NIIDQIASSK QTKQILLKRV
810 820 830 840 850
MRNMEHAMKE LGYLEYDHPE IAVTMKTKEE INVMLNMARE IVKAFRSKGI
860 870 880 890 900
IHKVEGTEIN KLIMAKKIQV LDLQSVIQPF NVEEAPCNIP WLSEDPEAIT
910 920 930 940 950
FIQEKAKVVT FDCGNNIFEE GDEPEGIYVI ISGMVKLKRS KPHLEMERVS
960 970 980 990 1000
AESEIKIHPL PHTEYLLSGE IIGELNCLTK ERMQYSATCK TVVETYFIPI
1010 1020 1030 1040 1050
SHLYEGFEKR CPNMKHKMWQ KIGLAITAQK IREHLSFEDW NYKLQLKLCN
1060 1070 1080 1090 1100
AFIRDIPKSM KTDIYDETVT HVVLIHGSAE DCQLRKIYKA PFLIPVTCHQ
1110 1120 1130 1140 1150
IQGMEDFTKV MIIQTSIAVR KFRWNVRKYI PPRRISMKPD SERESFETLD
1160 1170
ETSEEDNGKK ENQENEELIE ENINI
Length:1,175
Mass (Da):135,545
Last modified:July 27, 2011 - v3
Checksum:i4D65DAE082BF517F
GO

Sequence cautioni

The sequence AAQ88278.1 differs from that shown. Reason: Frameshift at position 29. Curated
The sequence DAA01464.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31M → T in AAQ88278. (PubMed:14634667)Curated
Sequence conflicti503 – 5031M → L in AAQ88278. (PubMed:14634667)Curated
Sequence conflicti520 – 5201N → T in AAQ88278. (PubMed:14634667)Curated
Sequence conflicti707 – 7071V → I in AAQ88278. (PubMed:14634667)Curated
Sequence conflicti844 – 8441A → T in AAQ88278. (PubMed:14634667)Curated
Sequence conflicti1129 – 11291Y → F in AAQ88278. (PubMed:14634667)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY368685 mRNA. Translation: AAQ88278.1. Frameshift.
AC124636 Genomic DNA. No translation available.
BK001330 mRNA. Translation: DAA01464.1. Sequence problems.
CCDSiCCDS28198.2.
RefSeqiNP_932774.3. NM_198106.4.
UniGeneiMm.277437.

Genome annotation databases

EnsembliENSMUST00000159945; ENSMUSP00000124969; ENSMUSG00000033210.
GeneIDi208169.
KEGGimmu:208169.
UCSCiuc007ziq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY368685 mRNA. Translation: AAQ88278.1 . Frameshift.
AC124636 Genomic DNA. No translation available.
BK001330 mRNA. Translation: DAA01464.1 . Sequence problems.
CCDSi CCDS28198.2.
RefSeqi NP_932774.3. NM_198106.4.
UniGenei Mm.277437.

3D structure databases

ProteinModelPortali Q6UJY2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60951N.
STRINGi 10090.ENSMUSP00000037782.

Protein family/group databases

TCDBi 2.A.36.7.7. the monovalent cation:proton antiporter-1 (cpa1) family.

PTM databases

PhosphoSitei Q6UJY2.

Proteomic databases

PRIDEi Q6UJY2.

Protocols and materials databases

DNASUi 208169.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000159945 ; ENSMUSP00000124969 ; ENSMUSG00000033210 .
GeneIDi 208169.
KEGGi mmu:208169.
UCSCi uc007ziq.2. mouse.

Organism-specific databases

CTDi 285335.
MGIi MGI:2685456. Slc9c1.

Phylogenomic databases

eggNOGi COG0025.
GeneTreei ENSGT00530000063840.
HOGENOMi HOG000169764.
HOVERGENi HBG071202.
InParanoidi Q6UJY2.
KOi K14726.
OMAi ASYQRQY.
OrthoDBi EOG7N37BW.
PhylomeDBi Q6UJY2.
TreeFami TF328865.

Enzyme and pathway databases

Reactomei REACT_196640. Stimuli-sensing channels.

Miscellaneous databases

NextBioi 372194.
PROi Q6UJY2.
SOURCEi Search...

Gene expression databases

Bgeei Q6UJY2.
CleanExi MM_SLC9A10.
ExpressionAtlasi Q6UJY2. baseline.
Genevestigatori Q6UJY2.

Family and domain databases

Gene3Di 1.20.120.350. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR027359. Channel_four-helix_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR028483. N/H_exchanger_10.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
PANTHERi PTHR10110. PTHR10110. 1 hit.
PTHR10110:SF87. PTHR10110:SF87. 1 hit.
Pfami PF00027. cNMP_binding. 1 hit.
PF00999. Na_H_Exchanger. 1 hit.
[Graphical view ]
SMARTi SM00100. cNMP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new sperm-specific Na+/H+ exchanger required for sperm motility and fertility."
    Wang D., King S.M., Quill T.A., Doolittle L.K., Garbers D.L.
    Nat. Cell Biol. 5:1117-1122(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: 129/SvEv.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs."
    Oduru S., Campbell J.L., Karri S., Hendry W.J., Khan S.A., Williams S.C.
    BMC Genomics 4:22-22(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. "A sperm-specific Na+/H+ exchanger (sNHE) is critical for expression and in vivo bicarbonate regulation of the soluble adenylyl cyclase (sAC)."
    Wang D., Hu J., Bobulescu I.A., Quill T.A., McLeroy P., Moe O.W., Garbers D.L.
    Proc. Natl. Acad. Sci. U.S.A. 104:9325-9330(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SAC.

Entry informationi

Entry nameiSL9C1_MOUSE
AccessioniPrimary (citable) accession number: Q6UJY2
Secondary accession number(s): E0CX34, Q7M6Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3