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Q6UJY2

- SL9C1_MOUSE

UniProt

Q6UJY2 - SL9C1_MOUSE

Protein

Sodium/hydrogen exchanger 10

Gene

Slc9c1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Sperm-specific sodium/hydrogen exchanger involved in intracellular pH regulation of spermatozoa. Required for sperm motility and fertility. Involved in sperm cell hyperactivation, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for the expression and bicarbonate regulation of the soluble adenylyl cyclase (sAC).2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi890 – 1026137cNMPAdd
    BLAST

    GO - Molecular functioni

    1. solute:proton antiporter activity Source: InterPro

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. sodium ion transport Source: UniProtKB-KW
    4. spermatogenesis Source: UniProtKB-KW
    5. sperm motility Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Antiport, Differentiation, Ion transport, Sodium transport, Spermatogenesis, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiREACT_196640. Stimuli-sensing channels.

    Protein family/group databases

    TCDBi2.A.36.7.7. the monovalent cation:proton antiporter-1 (cpa1) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/hydrogen exchanger 10
    Alternative name(s):
    Na(+)/H(+) exchanger 10
    Short name:
    NHE-10
    Solute carrier family 10 member 10
    Solute carrier family 9 member C1
    Sperm-specific Na(+)/H(+) exchanger
    Short name:
    sNHE
    Gene namesi
    Name:Slc9c1
    Synonyms:Gm610, Slc9a10
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:2685456. Slc9c1.

    Subcellular locationi

    Cell projectionciliumflagellum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. ciliary membrane Source: Reactome
    2. integral component of membrane Source: UniProtKB-KW
    3. motile cilium Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cilium, Flagellum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are normal but males are sterile. Male sterility is due to defects in sperm motility inability to fertilize intact eggs. Moreover, spermatozoa fail to develop the cAMP-dependent protein tyrosine phosphorylation that coincides with the functional maturation occurring upon incubation in capacitating conditions in vitro. cAMP analogs almost completely rescue the motility and infertility phenotypes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11751175Sodium/hydrogen exchanger 10PRO_0000295705Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ6UJY2.

    PTM databases

    PhosphoSiteiQ6UJY2.

    Expressioni

    Tissue specificityi

    Testis-specific. Specifically present in the principal piece of sperm tail (at protein level).1 Publication

    Gene expression databases

    BgeeiQ6UJY2.
    CleanExiMM_SLC9A10.
    GenevestigatoriQ6UJY2.

    Interactioni

    Subunit structurei

    Interacts with soluble adenylyl cyclase (sAC).1 Publication

    Protein-protein interaction databases

    DIPiDIP-60951N.
    STRINGi10090.ENSMUSP00000037782.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6UJY2.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei37 – 5721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei61 – 8121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei100 – 12021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei125 – 14521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei160 – 18021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei192 – 21221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei240 – 26021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei269 – 28921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei314 – 33421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei353 – 37321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei420 – 44021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei633 – 65321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei666 – 68621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei693 – 71321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni618 – 69881Ion transport-likeAdd
    BLAST

    Domaini

    The ion transport-like region is related to the membrane segments of voltage-gated ion channels. Its function is unknown.

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0025.
    GeneTreeiENSGT00530000063840.
    HOGENOMiHOG000169764.
    HOVERGENiHBG071202.
    InParanoidiQ6UJY2.
    KOiK14726.
    OMAiASYQRQY.
    OrthoDBiEOG7N37BW.
    PhylomeDBiQ6UJY2.
    TreeFamiTF328865.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR027359. Channel_four-helix_dom.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR028483. N/H_exchanger_10.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PANTHERiPTHR10110. PTHR10110. 1 hit.
    PTHR10110:SF87. PTHR10110:SF87. 1 hit.
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00999. Na_H_Exchanger. 1 hit.
    [Graphical view]
    SMARTiSM00100. cNMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6UJY2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEMEEISENL TASHSIKLTN MWLELLKSVF LSTPQDLPEI ILILSLICTV     50
    GAFLNMHLKD FPIPLPVILF LIGCCFEILS FASTQIQIYA DAIQWMDPDI 100
    FFGIFTPVII FNVAFDMDIY MLQKLFWQIL VITIPGFLIN YTLILWYLQS 150
    VNKLSLKTVP WLLFSAVLIS SDPMLTSASI RDLGLSRSLT NLINGESLLT 200
    SVLSLVIYSG VVHIRFKSKS VNHTLAHKVM STAWSYIVES FITGIVFTKV 250
    IQLWMATIFG DDVNHITLIF SVLYLIFYVC ELVGMSGIFT LATIGLFLNS 300
    TSFKPGVEAF LLEFWNCLSF IGFLMVFTFI GLLIPAHTYL HISFSDVYYS 350
    LNIYFTLIVL RLLVFLLMSP ILSRLGHGFS WRWAFIMVWS EMKGTPNINM 400
    ALLLAYSDIS LGSERERSQI LFHGVSVCVI TLIVNRFILP MAVTKLGLRD 450
    VTSTKYKSVY YTFQHFQELT KSTAMALKFD KDLANADWNM VDNAIILQNP 500
    YAMNQEEITE HQKVKCPDCN KEIDETLNIE AMELTNRRLL SAQIASYQRQ 550
    YRNEVLSQSA VQVLVGAAGS FGEKKGEYMS PENIKNFSES KKLLSFLRKL 600
    LLNWVYNTKK DKGVPSRYMF LHACHRIVFT NEFEYTGYLV VLMSTYPMII 650
    CWISRLKDIY DNEIKCANYY FLAFYILEAL LKVAAMRKEF FSHTWLLFEL 700
    GITLVGVLDI ILIETDSISY NFDLTETVVF MNVIRLLRIL RILKLVTPKL 750
    LQIIDKRMSQ QISFRYSILK GYVQGEMDVL NIIDQIASSK QTKQILLKRV 800
    MRNMEHAMKE LGYLEYDHPE IAVTMKTKEE INVMLNMARE IVKAFRSKGI 850
    IHKVEGTEIN KLIMAKKIQV LDLQSVIQPF NVEEAPCNIP WLSEDPEAIT 900
    FIQEKAKVVT FDCGNNIFEE GDEPEGIYVI ISGMVKLKRS KPHLEMERVS 950
    AESEIKIHPL PHTEYLLSGE IIGELNCLTK ERMQYSATCK TVVETYFIPI 1000
    SHLYEGFEKR CPNMKHKMWQ KIGLAITAQK IREHLSFEDW NYKLQLKLCN 1050
    AFIRDIPKSM KTDIYDETVT HVVLIHGSAE DCQLRKIYKA PFLIPVTCHQ 1100
    IQGMEDFTKV MIIQTSIAVR KFRWNVRKYI PPRRISMKPD SERESFETLD 1150
    ETSEEDNGKK ENQENEELIE ENINI 1175
    Length:1,175
    Mass (Da):135,545
    Last modified:July 27, 2011 - v3
    Checksum:i4D65DAE082BF517F
    GO

    Sequence cautioni

    The sequence AAQ88278.1 differs from that shown. Reason: Frameshift at position 29.
    The sequence DAA01464.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31M → T in AAQ88278. (PubMed:14634667)Curated
    Sequence conflicti503 – 5031M → L in AAQ88278. (PubMed:14634667)Curated
    Sequence conflicti520 – 5201N → T in AAQ88278. (PubMed:14634667)Curated
    Sequence conflicti707 – 7071V → I in AAQ88278. (PubMed:14634667)Curated
    Sequence conflicti844 – 8441A → T in AAQ88278. (PubMed:14634667)Curated
    Sequence conflicti1129 – 11291Y → F in AAQ88278. (PubMed:14634667)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY368685 mRNA. Translation: AAQ88278.1. Frameshift.
    AC124636 Genomic DNA. No translation available.
    BK001330 mRNA. Translation: DAA01464.1. Sequence problems.
    CCDSiCCDS28198.2.
    RefSeqiNP_932774.3. NM_198106.4.
    UniGeneiMm.277437.

    Genome annotation databases

    EnsembliENSMUST00000159945; ENSMUSP00000124969; ENSMUSG00000033210.
    GeneIDi208169.
    KEGGimmu:208169.
    UCSCiuc007ziq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY368685 mRNA. Translation: AAQ88278.1 . Frameshift.
    AC124636 Genomic DNA. No translation available.
    BK001330 mRNA. Translation: DAA01464.1 . Sequence problems.
    CCDSi CCDS28198.2.
    RefSeqi NP_932774.3. NM_198106.4.
    UniGenei Mm.277437.

    3D structure databases

    ProteinModelPortali Q6UJY2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60951N.
    STRINGi 10090.ENSMUSP00000037782.

    Protein family/group databases

    TCDBi 2.A.36.7.7. the monovalent cation:proton antiporter-1 (cpa1) family.

    PTM databases

    PhosphoSitei Q6UJY2.

    Proteomic databases

    PRIDEi Q6UJY2.

    Protocols and materials databases

    DNASUi 208169.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000159945 ; ENSMUSP00000124969 ; ENSMUSG00000033210 .
    GeneIDi 208169.
    KEGGi mmu:208169.
    UCSCi uc007ziq.2. mouse.

    Organism-specific databases

    CTDi 285335.
    MGIi MGI:2685456. Slc9c1.

    Phylogenomic databases

    eggNOGi COG0025.
    GeneTreei ENSGT00530000063840.
    HOGENOMi HOG000169764.
    HOVERGENi HBG071202.
    InParanoidi Q6UJY2.
    KOi K14726.
    OMAi ASYQRQY.
    OrthoDBi EOG7N37BW.
    PhylomeDBi Q6UJY2.
    TreeFami TF328865.

    Enzyme and pathway databases

    Reactomei REACT_196640. Stimuli-sensing channels.

    Miscellaneous databases

    NextBioi 372194.
    PROi Q6UJY2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6UJY2.
    CleanExi MM_SLC9A10.
    Genevestigatori Q6UJY2.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR027359. Channel_four-helix_dom.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR028483. N/H_exchanger_10.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    PANTHERi PTHR10110. PTHR10110. 1 hit.
    PTHR10110:SF87. PTHR10110:SF87. 1 hit.
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00999. Na_H_Exchanger. 1 hit.
    [Graphical view ]
    SMARTi SM00100. cNMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new sperm-specific Na+/H+ exchanger required for sperm motility and fertility."
      Wang D., King S.M., Quill T.A., Doolittle L.K., Garbers D.L.
      Nat. Cell Biol. 5:1117-1122(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: 129/SvEv.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs."
      Oduru S., Campbell J.L., Karri S., Hendry W.J., Khan S.A., Williams S.C.
      BMC Genomics 4:22-22(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    4. "A sperm-specific Na+/H+ exchanger (sNHE) is critical for expression and in vivo bicarbonate regulation of the soluble adenylyl cyclase (sAC)."
      Wang D., Hu J., Bobulescu I.A., Quill T.A., McLeroy P., Moe O.W., Garbers D.L.
      Proc. Natl. Acad. Sci. U.S.A. 104:9325-9330(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SAC.

    Entry informationi

    Entry nameiSL9C1_MOUSE
    AccessioniPrimary (citable) accession number: Q6UJY2
    Secondary accession number(s): E0CX34, Q7M6Y7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 86 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3