Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6UJY2 (SL9C1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/hydrogen exchanger 10
Alternative name(s):
Na(+)/H(+) exchanger 10
Short name=NHE-10
Solute carrier family 10 member 10
Solute carrier family 9 member C1
Sperm-specific Na(+)/H(+) exchanger
Short name=sNHE
Gene names
Name:Slc9c1
Synonyms:Gm610, Slc9a10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1175 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sperm-specific sodium/hydrogen exchanger involved in intracellular pH regulation of spermatozoa. Required for sperm motility and fertility. Involved in sperm cell hyperactivation, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for the expression and bicarbonate regulation of the soluble adenylyl cyclase (sAC). Ref.1 Ref.4

Subunit structure

Interacts with soluble adenylyl cyclase (sAC). Ref.4

Subcellular location

Cell projectionciliumflagellum membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Testis-specific. Specifically present in the principal piece of sperm tail (at protein level). Ref.1

Domain

The ion transport-like region is related to the membrane segments of voltage-gated ion channels. Its function is unknown.

Disruption phenotype

Mice are normal but males are sterile. Male sterility is due to defects in sperm motility inability to fertilize intact eggs. Moreover, spermatozoa fail to develop the cAMP-dependent protein tyrosine phosphorylation that coincides with the functional maturation occurring upon incubation in capacitating conditions in vitro. cAMP analogs almost completely rescue the motility and infertility phenotypes. Ref.1

Sequence similarities

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Sequence caution

The sequence AAQ88278.1 differs from that shown. Reason: Frameshift at position 29.

The sequence DAA01464.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11751175Sodium/hydrogen exchanger 10
PRO_0000295705

Regions

Transmembrane37 – 5721Helical; Potential
Transmembrane61 – 8121Helical; Potential
Transmembrane100 – 12021Helical; Potential
Transmembrane125 – 14521Helical; Potential
Transmembrane160 – 18021Helical; Potential
Transmembrane192 – 21221Helical; Potential
Transmembrane240 – 26021Helical; Potential
Transmembrane269 – 28921Helical; Potential
Transmembrane314 – 33421Helical; Potential
Transmembrane353 – 37321Helical; Potential
Transmembrane420 – 44021Helical; Potential
Transmembrane633 – 65321Helical; Potential
Transmembrane666 – 68621Helical; Potential
Transmembrane693 – 71321Helical; Potential
Nucleotide binding890 – 1026137cNMP
Region618 – 69881Ion transport-like

Amino acid modifications

Glycosylation2221N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict31M → T in AAQ88278. Ref.1
Sequence conflict5031M → L in AAQ88278. Ref.1
Sequence conflict5201N → T in AAQ88278. Ref.1
Sequence conflict7071V → I in AAQ88278. Ref.1
Sequence conflict8441A → T in AAQ88278. Ref.1
Sequence conflict11291Y → F in AAQ88278. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6UJY2 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 4D65DAE082BF517F

FASTA1,175135,545
        10         20         30         40         50         60 
MEMEEISENL TASHSIKLTN MWLELLKSVF LSTPQDLPEI ILILSLICTV GAFLNMHLKD 

        70         80         90        100        110        120 
FPIPLPVILF LIGCCFEILS FASTQIQIYA DAIQWMDPDI FFGIFTPVII FNVAFDMDIY 

       130        140        150        160        170        180 
MLQKLFWQIL VITIPGFLIN YTLILWYLQS VNKLSLKTVP WLLFSAVLIS SDPMLTSASI 

       190        200        210        220        230        240 
RDLGLSRSLT NLINGESLLT SVLSLVIYSG VVHIRFKSKS VNHTLAHKVM STAWSYIVES 

       250        260        270        280        290        300 
FITGIVFTKV IQLWMATIFG DDVNHITLIF SVLYLIFYVC ELVGMSGIFT LATIGLFLNS 

       310        320        330        340        350        360 
TSFKPGVEAF LLEFWNCLSF IGFLMVFTFI GLLIPAHTYL HISFSDVYYS LNIYFTLIVL 

       370        380        390        400        410        420 
RLLVFLLMSP ILSRLGHGFS WRWAFIMVWS EMKGTPNINM ALLLAYSDIS LGSERERSQI 

       430        440        450        460        470        480 
LFHGVSVCVI TLIVNRFILP MAVTKLGLRD VTSTKYKSVY YTFQHFQELT KSTAMALKFD 

       490        500        510        520        530        540 
KDLANADWNM VDNAIILQNP YAMNQEEITE HQKVKCPDCN KEIDETLNIE AMELTNRRLL 

       550        560        570        580        590        600 
SAQIASYQRQ YRNEVLSQSA VQVLVGAAGS FGEKKGEYMS PENIKNFSES KKLLSFLRKL 

       610        620        630        640        650        660 
LLNWVYNTKK DKGVPSRYMF LHACHRIVFT NEFEYTGYLV VLMSTYPMII CWISRLKDIY 

       670        680        690        700        710        720 
DNEIKCANYY FLAFYILEAL LKVAAMRKEF FSHTWLLFEL GITLVGVLDI ILIETDSISY 

       730        740        750        760        770        780 
NFDLTETVVF MNVIRLLRIL RILKLVTPKL LQIIDKRMSQ QISFRYSILK GYVQGEMDVL 

       790        800        810        820        830        840 
NIIDQIASSK QTKQILLKRV MRNMEHAMKE LGYLEYDHPE IAVTMKTKEE INVMLNMARE 

       850        860        870        880        890        900 
IVKAFRSKGI IHKVEGTEIN KLIMAKKIQV LDLQSVIQPF NVEEAPCNIP WLSEDPEAIT 

       910        920        930        940        950        960 
FIQEKAKVVT FDCGNNIFEE GDEPEGIYVI ISGMVKLKRS KPHLEMERVS AESEIKIHPL 

       970        980        990       1000       1010       1020 
PHTEYLLSGE IIGELNCLTK ERMQYSATCK TVVETYFIPI SHLYEGFEKR CPNMKHKMWQ 

      1030       1040       1050       1060       1070       1080 
KIGLAITAQK IREHLSFEDW NYKLQLKLCN AFIRDIPKSM KTDIYDETVT HVVLIHGSAE 

      1090       1100       1110       1120       1130       1140 
DCQLRKIYKA PFLIPVTCHQ IQGMEDFTKV MIIQTSIAVR KFRWNVRKYI PPRRISMKPD 

      1150       1160       1170 
SERESFETLD ETSEEDNGKK ENQENEELIE ENINI 

« Hide

References

« Hide 'large scale' references
[1]"A new sperm-specific Na+/H+ exchanger required for sperm motility and fertility."
Wang D., King S.M., Quill T.A., Doolittle L.K., Garbers D.L.
Nat. Cell Biol. 5:1117-1122(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
Strain: 129/SvEv.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs."
Oduru S., Campbell J.L., Karri S., Hendry W.J., Khan S.A., Williams S.C.
BMC Genomics 4:22-22(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[4]"A sperm-specific Na+/H+ exchanger (sNHE) is critical for expression and in vivo bicarbonate regulation of the soluble adenylyl cyclase (sAC)."
Wang D., Hu J., Bobulescu I.A., Quill T.A., McLeroy P., Moe O.W., Garbers D.L.
Proc. Natl. Acad. Sci. U.S.A. 104:9325-9330(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SAC.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY368685 mRNA. Translation: AAQ88278.1. Frameshift.
AC124636 Genomic DNA. No translation available.
BK001330 mRNA. Translation: DAA01464.1. Sequence problems.
CCDSCCDS28198.2.
RefSeqNP_932774.3. NM_198106.4.
UniGeneMm.277437.

3D structure databases

ProteinModelPortalQ6UJY2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000037782.

Protein family/group databases

TCDB2.A.36.7.7. the monovalent cation:proton antiporter-1 (cpa1) family.

PTM databases

PhosphoSiteQ6UJY2.

Proteomic databases

PRIDEQ6UJY2.

Protocols and materials databases

DNASU208169.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000159945; ENSMUSP00000124969; ENSMUSG00000033210.
GeneID208169.
KEGGmmu:208169.
UCSCuc007ziq.2. mouse.

Organism-specific databases

CTD285335.
MGIMGI:2685456. Slc9c1.

Phylogenomic databases

eggNOGCOG0025.
GeneTreeENSGT00530000063840.
HOGENOMHOG000169764.
HOVERGENHBG071202.
InParanoidQ6UJY2.
KOK14726.
OMAASYQRQY.
OrthoDBEOG7N37BW.
PhylomeDBQ6UJY2.
TreeFamTF328865.

Gene expression databases

BgeeQ6UJY2.
CleanExMM_SLC9A10.
GenevestigatorQ6UJY2.

Family and domain databases

Gene3D1.20.120.350. 1 hit.
2.60.120.10. 1 hit.
InterProIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR027359. Channel_four-helix_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR028483. N/H_exchanger_10.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF87. PTHR10110:SF87. 1 hit.
PfamPF00027. cNMP_binding. 1 hit.
PF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio372194.
PROQ6UJY2.
SOURCESearch...

Entry information

Entry nameSL9C1_MOUSE
AccessionPrimary (citable) accession number: Q6UJY2
Secondary accession number(s): E0CX34, Q7M6Y7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 84 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot