Q6UGB2 (MSMO1_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylsterol monooxygenase 1
EC=1.14.13.72

Alternative name(s):
C-4 methylsterol oxidase

Gene names

Name:	MSMO1
Synonyms:	SC4MOL

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O. 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O. 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O.
Cofactor	Iron By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the sterol desaturase family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Iron NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: InterPro sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	C-4 methylsterol oxidase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Methylsterol monooxygenase 1		PRO_0000249851
Regions
	Transmembrane	55 – 75	21	Helical; Potential
	Transmembrane	100 – 120	21	Helical; Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Motif	157 – 161	5	Histidine box-1
	Motif	170 – 174	5	Histidine box-2
	Motif	249 – 255	7	Histidine box-3

Sequences

	Sequence		Length	Mass (Da)
	Q6UGB2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 02B4733E8049F0ED	FASTA	293	35,102
	10 20 30 40 50 60 MATNQSISIF SSASLAVEYV DSLLPENPLQ EPFKNAWNYM LNNYTKFQIA TWGSLIVHEA 70 80 90 100 110 120 LYFFFCLPGF LFQFIPYMKK YKIQKDKPET WENQWKCFKV LLFNHFCIQF PLICGTYYFT 130 140 150 160 170 180 EYFSIPYDWE TMPRWYIALA RCFGCAVIED TWHYFLHRLL HHKRIYKYIH KIHHEFQAPF 190 200 210 220 230 240 GMEAEYAHPL ETLILGTGFF IGIMLLCDHV ILLWAWVTVR LIETIDVHSG YDIPLNPLHL 250 260 270 280 290 IPFYAGSRHH DFHHMNFIGN YASTFTWWDR IFGTDAQYNA YNEKRKKAEK KTE

References

[1]	Hwang K.-C., Ok D.-W., Kwon D.-N., Choi Y.-J., Lee S.-Y., Kim J.-H. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY370690 mRNA. Translation: AAQ67416.1.
RefSeq	NP_998917.1. NM_213752.1.
UniGene	Ssc.18628.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6UGB2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396590.
KEGG	ssc:396590.
Organism-specific databases
CTD	6307.
Phylogenomic databases
GeneTree	ENSGT00530000063017.
HOVERGEN	HBG051504.
OrthoDB	EOG4CG08K.
Family and domain databases
InterPro	IPR006694. Fatty_acid_hydroxylase. [Graphical view]
KO	K07750.
Pfam	PF04116. FA_hydroxylase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSMO1_PIG

Accession

Primary (citable) accession number: Q6UGB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program