Reviewed, UniProtKB/Swiss-Prot Q6UE39 (GLT13_RAT)

Names and origin

Protein names

Recommended name:
    Polypeptide N-acetylgalactosaminyltransferase 13
    EC=2.4.1.41
Alternative name(s):
    Polypeptide GalNAc transferase 13
      Short name=pp-GaNTase 13
      Short name=GalNAc-T13
    Protein-UDP acetylgalactosaminyltransferase 13
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 13

Gene names

Name:

Galnt13

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a much stronger activity than GALNT1 to transfer GalNAc to mucin peptides, such as Muc5Ac and Muc7. Able to glycosylate SDC3. May be responsible for the synthesis of Tn antigen in neuronal cells By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + polypeptide = UDP + N-acetyl-D-galactosaminyl-polypeptide.
Cofactor	Manganese By similarity. Calcium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Domain	There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Calcium Lectin Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW polypeptide N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Polypeptide N-acetylgalactosaminyltransferase 13		PRO_0000059132
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 27	23	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 556	529	Lumenal Potential
	Domain	428 – 550	123	Ricin B-type lectin
	Region	114 – 224	111	Catalytic subdomain A
	Region	284 – 346	63	Catalytic subdomain B
Amino acid modifications
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	551	1	N-linked (GlcNAc...) Potential
	Disulfide bond	441 ↔ 458		By similarity
	Disulfide bond	481 ↔ 496		By similarity
	Disulfide bond	522 ↔ 539		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6UE39-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5AEEB9F0F4ECD148	FASTA	556	63,949
	10 20 30 40 50 60 MRRLVYCKVV LATSLMWVLV DVFLLLYFSE CNKCDDKKER SLLPALRAVI SRNQEGPGEM 70 80 90 100 110 120 GKAVLIPKDD QEKMKELFKI NQFNLMASDL IALNRSLPDV RLEGCKTKVY PDELPNTSVV 130 140 150 160 170 180 IVFHNEAWST LLRTVYSVIN RSPHYLLSEV ILVDDASERD FLKLTLENYV KTLEVPVKII 190 200 210 220 230 240 RMEERSGLIR ARLRGAAASK GQVITFLDAH CECTLGWLEP LLARIKEDRK TVVCPIIDVI 250 260 270 280 290 300 SDDTFEYMAG SDMTYGGFNW KLNFRWYPVP QREMDRRKGD RTLPVRTPTM AGGLFSIDRN 310 320 330 340 350 360 YFEEIGTYDA GMDIWGGENL EMSFRIWQCG GSLEIVTCSH VGHVFRKATP YTFPGGTGHV 370 380 390 400 410 420 INKNNRRLAE VWMDEFKDFF YIISPGVVKV DYGDVSVRKT LRENLKCKPF SWYLENIYPD 430 440 450 460 470 480 SQIPRRYYSL GEIRNVETNQ CLDNMGRKEN EKVGIFNCHG MGGNQVFSYT ADKEIRTDDL 490 500 510 520 530 540 CLDVSRLSGP VIMLKCHHMR GNQLWEYDAE RLTLRHANSN QCLDEPSEED KMVPTMQDCS 550 GSRSQQWLLR NMTLGT

References

[1]	Huang C.Q., Wu S.L., Liu S. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY371923 mRNA. Translation: AAQ75749.1.
IPI	IPI00400577.
RefSeq	NP_954537.1.
UniGene	Rn.39371
3D structure databases
SMR	Q6UE39. Positions 94-552.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6UE39.
Protein family/group databases
CAZy	CBM13. Carbohydrate-Binding Module Family 13. GT27. Glycosyltransferase Family 27.
Genome annotation databases
Ensembl	ENSRNOT00000007108; ENSRNOP00000007108; ENSRNOG00000005335; Rattus norvegicus. [Genome view] ENSRNOT00000048804; ENSRNOP00000042772; ENSRNOG00000005335; Rattus norvegicus. [Genome view]
GeneID	311039.
KEGG	rno:311039.
UCSC	NM_199106. rat.
Organism-specific databases
CTD	311039.
RGD	735044. Galnt13.
Phylogenomic databases
HOVERGEN	Q6UE39.
Enzyme and pathway databases
BRENDA	2.4.1.41. 248.
Gene expression databases
ArrayExpress	Q6UE39.
Genevestigator	Q6UE39.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
PROSITE	PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	662923.

Entry information

Entry name

GLT13_RAT

Accession

Primary (citable) accession number: Q6UE39

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	October 13, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)