Q6UDJ2 (RIR1_PSHV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase large subunit
Short name=R1
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase large subunit

Gene names

Name:

UL39

Organism

Psittacid herpesvirus 1 (isolate Amazon parrot/-/97-0001/1997) (PsHV-1) (Pacheco's disease virus) [Reference proteome]

Taxonomic identifier

670426 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Iltovirus › Psittacid herpesvirus 1

Virus host

Amazona oratrix (yellow-headed parrot) [TaxID: 152276]

Protein attributes

Sequence length	811 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. The N-terminal region confers antiapoptotic activity in differentiated cells such as neurons and is important for viral reactivation to increase neural survivability By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large subunit UL39 and a small subunit UL40 By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
Biological process	DNA replication Host-virus interaction
Developmental stage	Early protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 811	811	Ribonucleoside-diphosphate reductase large subunit		PRO_0000406807
Regions
	Region	246 – 247	2	Substrate binding By similarity
	Region	450 – 454	5	Substrate binding By similarity
	Region	636 – 640	5	Substrate binding By similarity
Sites
	Active site	450	1	Proton acceptor By similarity
	Active site	452	1	Cysteine radical intermediate By similarity
	Active site	454	1	Proton acceptor By similarity
	Binding site	231	1	Substrate By similarity
	Binding site	277	1	Substrate; via amide nitrogen By similarity
	Site	247	1	Important for hydrogen atom transfer By similarity
	Site	467	1	Important for hydrogen atom transfer By similarity
	Site	781	1	Important for electron transfer By similarity
	Site	782	1	Important for electron transfer By similarity
	Site	807	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	810	1	Interacts with thioredoxin/glutaredoxin By similarity
Amino acid modifications
	Disulfide bond	247 ↔ 467		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6UDJ2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 92166F6616E4BE9C	FASTA	811	90,420
	10 20 30 40 50 60 MSSATSLVPP AAPDMREHEL GECAAFYAAE TPQRLFENLL HLENALKARG YDTDSAGAPA 70 80 90 100 110 120 LGPTTLTMEA IADRITLIIN RFKAAVRLDL ELYRLLAELV HIRIRTKTVS MQAWIELRGL 130 140 150 160 170 180 SRECAEFILE RKNFVCELME RFGEVYPTLS RVGLQSARKF ESMYLGKLKN GRLESVGQFF 190 200 210 220 230 240 LRIAAEAARG VANNDAFAAA VFRDGTRAPD ANTVFCLFFM ALCRQEIVPP TPVMLFAGTE 250 260 270 280 290 300 SRSYASCFLL DVRGRHTRDV LTSIAEEIIP VMHSHGGIGL YMDCDSNWDD NSSGMMLALK 310 320 330 340 350 360 ALDSIIAASN AVSARPSGLC VYVEPWHRDI MKILRCRGVL AGNEETRCDN TFFALWMPDL 370 380 390 400 410 420 FMKRFEANGT WTLFDGRAAH LSDLYGEEFE KEYELLERKN VGIATYPARD VMFALIKSAV 430 440 450 460 470 480 STGTPFVMFK HAVNRNYFFD MAGRAMKCSN LCTEIVHMTD DESVGVCNLT SLNLAAFVTR 490 500 510 520 530 540 RNALPGTPPI GTFDYSSFRD ACAVATVFIN ALMSLSNLPI KRATTGNERL RSIGIGVQGF 550 560 570 580 590 600 HTACLLQGFG LDSVEACRFN GKLFEALALT TFQTSCRICE LGMNPFRGFS ESKYAKGWLH 610 620 630 640 650 660 MDGWPARHLY FDGWDRLREN IKAYGLYNCQ LVALMPTASS SQLTEVSEGI HPVFGNIFSK 670 680 690 700 710 720 ITTTGEDIQL NVALMETIEC LYPNKAERRD ILERLHKNKW STRGAFGAAL PSQHPLTKFD 730 740 750 760 770 780 TAFEADQEHL LRLSADRAPF VDHSQSTTLY VVEEDDGAVR ASRVAHLLTT AFKYGLKTGM 790 800 810 YYCKVRKATD NGVFLGTDTC RRDDPTCLAC Q

References

[1]	"Psittacid herpesvirus 1 and infectious laryngotracheitis virus: Comparative genome sequence analysis of two avian alphaherpesviruses." Thureen D.R., Keeler C.L. Jr. J. Virol. 80:7863-7872(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY372243 Genomic DNA. Translation: AAQ73718.1.
RefSeq	NP_944412.1. NC_005264.1.
3D structure databases
ProteinModelPortal	Q6UDJ2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2656977.
Phylogenomic databases
ProtClustDB	CLSP2509601.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
InterPro	IPR013346. NrdE_NrdA. IPR000788. RNR_lg_C. IPR013509. RNR_lsu_N. [Graphical view]
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_PSHV1

Accession

Primary (citable) accession number: Q6UDJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program