Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6UB28

- MAP12_HUMAN

UniProt

Q6UB28 - MAP12_HUMAN

Protein

Methionine aminopeptidase 1D, mitochondrial

Gene

METAP1D

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed By similarity. May play a role in colon tumorigenesis.1 PublicationUniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Kineticsi

    1. KM=573 µM for Met-pro-p-nitroanilide (at pH 8)1 Publication

    pH dependencei

    Optimum pH is 7.5-8.0.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei161 – 1611SubstrateUniRule annotation
    Metal bindingi178 – 1781Divalent metal cation 1UniRule annotation
    Metal bindingi189 – 1891Divalent metal cation 1UniRule annotation
    Metal bindingi189 – 1891Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi252 – 2521Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei259 – 2591SubstrateUniRule annotation
    Metal bindingi284 – 2841Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi315 – 3151Divalent metal cation 1UniRule annotation
    Metal bindingi315 – 3151Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-HAMAP
    3. metalloaminopeptidase activity Source: UniProtKB-HAMAP
    4. metalloexopeptidase activity Source: UniProtKB

    GO - Biological processi

    1. N-terminal protein amino acid modification Source: HGNC
    2. peptidyl-methionine modification Source: HGNC
    3. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Enzyme and pathway databases

    BRENDAi3.4.11.18. 2681.
    SABIO-RKQ6UB28.

    Protein family/group databases

    MEROPSiM24.028.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 1D, mitochondrialUniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 1DUniRule annotation
    Short name:
    MetAP 1DUniRule annotation
    Alternative name(s):
    Methionyl aminopeptidase type 1D, mitochondrial
    Peptidase M 1DUniRule annotation
    Gene namesi
    Name:METAP1D
    Synonyms:MAP1D
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:32583. METAP1D.

    Subcellular locationi

    Mitochondrion 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: HGNC

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1919MitochondrionUniRule annotationAdd
    BLAST
    Chaini20 – 335316Methionine aminopeptidase 1D, mitochondrialPRO_0000314126Add
    BLAST

    Proteomic databases

    MaxQBiQ6UB28.
    PaxDbiQ6UB28.
    PRIDEiQ6UB28.

    PTM databases

    PhosphoSiteiQ6UB28.

    Expressioni

    Tissue specificityi

    Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level).1 Publication

    Gene expression databases

    BgeeiQ6UB28.
    GenevestigatoriQ6UB28.

    Organism-specific databases

    HPAiHPA030299.

    Interactioni

    Protein-protein interaction databases

    BioGridi129008. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6UB28.
    SMRiQ6UB28. Positions 63-330.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0024.
    HOGENOMiHOG000030427.
    HOVERGENiHBG067178.
    InParanoidiQ6UB28.
    KOiK01265.
    OMAiLNHIYLH.
    OrthoDBiEOG786H38.
    PhylomeDBiQ6UB28.
    TreeFamiTF325318.

    Family and domain databases

    Gene3Di3.90.230.10. 1 hit.
    HAMAPiMF_01974. MetAP_1.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002467. Pept_M24A_MAP1.
    [Graphical view]
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 1 hit.
    TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
    PROSITEiPS00680. MAP_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6UB28-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAPSGVHLL VRRGSHRIFS SPLNHIYLHK QSSSQQRRNF FFRRQRDISH    50
    SIVLPAAVSS AHPVPKHIKK PDYVTTGIVP DWGDSIEVKN EDQIQGLHQA 100
    CQLARHVLLL AGKSLKVDMT TEEIDALVHR EIISHNAYPS PLGYGGFPKS 150
    VCTSVNNVLC HGIPDSRPLQ DGDIINIDVT VYYNGYHGDT SETFLVGNVD 200
    ECGKKLVEVA RRCRDEAIAA CRAGAPFSVI GNTISHITHQ NGFQVCPHFV 250
    GHGIGSYFHG HPEIWHHAND SDLPMEEGMA FTIEPIITEG SPEFKVLEDA 300
    WTVVSLDNQR SAQFEHTVLI TSRGAQILTK LPHEA 335
    Length:335
    Mass (Da):37,088
    Last modified:July 5, 2004 - v1
    Checksum:i2EB90946DC983D7B
    GO

    Sequence cautioni

    The sequence AAY55948.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti14 – 141G → V.1 Publication
    Corresponds to variant rs10497377 [ dbSNP | Ensembl ].
    VAR_050273

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY374142 mRNA. Translation: AAR27795.1.
    DQ005576 mRNA. Translation: AAY55948.1. Different initiation.
    CH471058 Genomic DNA. Translation: EAX11190.1.
    BC113644 mRNA. Translation: AAI13645.1.
    CCDSiCCDS2246.1.
    RefSeqiNP_954697.1. NM_199227.1.
    UniGeneiHs.298250.

    Genome annotation databases

    EnsembliENST00000315796; ENSP00000315152; ENSG00000172878.
    GeneIDi254042.
    KEGGihsa:254042.
    UCSCiuc002uhk.3. human.

    Polymorphism databases

    DMDMi74710242.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY374142 mRNA. Translation: AAR27795.1 .
    DQ005576 mRNA. Translation: AAY55948.1 . Different initiation.
    CH471058 Genomic DNA. Translation: EAX11190.1 .
    BC113644 mRNA. Translation: AAI13645.1 .
    CCDSi CCDS2246.1.
    RefSeqi NP_954697.1. NM_199227.1.
    UniGenei Hs.298250.

    3D structure databases

    ProteinModelPortali Q6UB28.
    SMRi Q6UB28. Positions 63-330.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 129008. 2 interactions.

    Protein family/group databases

    MEROPSi M24.028.

    PTM databases

    PhosphoSitei Q6UB28.

    Polymorphism databases

    DMDMi 74710242.

    Proteomic databases

    MaxQBi Q6UB28.
    PaxDbi Q6UB28.
    PRIDEi Q6UB28.

    Protocols and materials databases

    DNASUi 254042.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000315796 ; ENSP00000315152 ; ENSG00000172878 .
    GeneIDi 254042.
    KEGGi hsa:254042.
    UCSCi uc002uhk.3. human.

    Organism-specific databases

    CTDi 254042.
    GeneCardsi GC02P172865.
    HGNCi HGNC:32583. METAP1D.
    HPAi HPA030299.
    MIMi 610267. gene.
    neXtProti NX_Q6UB28.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0024.
    HOGENOMi HOG000030427.
    HOVERGENi HBG067178.
    InParanoidi Q6UB28.
    KOi K01265.
    OMAi LNHIYLH.
    OrthoDBi EOG786H38.
    PhylomeDBi Q6UB28.
    TreeFami TF325318.

    Enzyme and pathway databases

    BRENDAi 3.4.11.18. 2681.
    SABIO-RK Q6UB28.

    Miscellaneous databases

    ChiTaRSi METAP1D. human.
    GenomeRNAii 254042.
    NextBioi 92234.
    PROi Q6UB28.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6UB28.
    Genevestigatori Q6UB28.

    Family and domain databases

    Gene3Di 3.90.230.10. 1 hit.
    HAMAPi MF_01974. MetAP_1.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002467. Pept_M24A_MAP1.
    [Graphical view ]
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 1 hit.
    TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
    PROSITEi PS00680. MAP_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An unusual peptide deformylase features in the human mitochondrial N-terminal methionine excision pathway."
      Serero A., Giglione C., Sardini A., Martinez-Sanz J., Meinnel T.
      J. Biol. Chem. 278:52953-52963(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    2. "MAP1D, a novel methionine aminopeptidase family member is overexpressed in colon cancer."
      Leszczyniecka M., Bhatia U., Cueto M., Nirmala N.R., Towbin H., Vattay A., Wang B., Zabludoff S., Phillips P.E.
      Oncogene 25:3471-3478(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANT VAL-14.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Kinetic and mutational studies of the number of interacting divalent cations required by bacterial and human methionine aminopeptidases."
      Hu X.V., Chen X., Han K.C., Mildvan A.S., Liu J.O.
      Biochemistry 46:12833-12843(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiMAP12_HUMAN
    AccessioniPrimary (citable) accession number: Q6UB28
    Secondary accession number(s): Q1WNX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or a Met upstream of this sequence is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3