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Protein

Zinc finger protein 322

Gene

ZNF322

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator (PubMed:15555580). Important for maintenance of pluripotency in embryonic stem cells (By similarity). Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes (By similarity). Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly (By similarity). Promotes inhibition of MAPK signaling during embryonic stem cell differentiation (By similarity).By similarity1 Publication

Miscellaneous

Significantly enhances POU5F1/OCT4-SOX2-KLF4-MYC (OSKM) mediated reprogramming of mouse embryonic fibroblasts into induced pluripotent stem cells, and can also substitute for SOX2 in this process.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri43 – 65C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri71 – 93C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri99 – 121C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri127 – 149C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri155 – 177C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri183 – 205C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri211 – 233C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri239 – 261C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri267 – 289C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri293 – 315C2H2-type 10; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri351 – 373C2H2-type 11; degeneratePROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 322
Alternative name(s):
Zinc finger protein 322A
Zinc finger protein 388
Zinc finger protein 489
Gene namesi
Name:ZNF322
Synonyms:ZNF322A, ZNF388, ZNF489
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000181315.10
HGNCiHGNC:23640 ZNF322
MIMi610847 gene
neXtProtiNX_Q6U7Q0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi79692
OpenTargetsiENSG00000181315
PharmGKBiPA134861342

Polymorphism and mutation databases

BioMutaiZNF322
DMDMi82582384

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000475291 – 402Zinc finger protein 322Add BLAST402

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei391PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6U7Q0
PaxDbiQ6U7Q0
PeptideAtlasiQ6U7Q0
PRIDEiQ6U7Q0

PTM databases

iPTMnetiQ6U7Q0
PhosphoSitePlusiQ6U7Q0

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in heart and skeletal muscle.1 Publication

Developmental stagei

Expressed in embryo as early as the 11th week of gestation.1 Publication

Gene expression databases

BgeeiENSG00000181315
CleanExiHS_ZNF322A
ExpressionAtlasiQ6U7Q0 baseline and differential
GenevisibleiQ6U7Q0 HS

Organism-specific databases

HPAiHPA043161
HPA046692

Interactioni

Subunit structurei

Interacts with POU5F1.By similarity

Protein-protein interaction databases

BioGridi122813, 1 interactor
STRINGi9606.ENSP00000418897

Structurei

3D structure databases

ProteinModelPortaliQ6U7Q0
SMRiQ6U7Q0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri43 – 65C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri71 – 93C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri99 – 121C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri127 – 149C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri155 – 177C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri183 – 205C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri211 – 233C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri239 – 261C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri267 – 289C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri293 – 315C2H2-type 10; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri351 – 373C2H2-type 11; degeneratePROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00910000144036
HOGENOMiHOG000234617
HOVERGENiHBG018163
InParanoidiQ6U7Q0
OMAiVCPQKGK
OrthoDBiEOG091G02KC
PhylomeDBiQ6U7Q0
TreeFamiTF338126

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 11 hits
SUPFAMiSSF57667 SSF57667, 7 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 8 hits
PS50157 ZINC_FINGER_C2H2_2, 11 hits

Sequencei

Sequence statusi: Complete.

Q6U7Q0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTSEEKCNQ RTQKRKIYNV CPRKGKKIFI HMHEIIQIDG HIYQCLECKQ
60 70 80 90 100
NFCENLALIM CERTHTGEKP YKCDMCEKTF VQSSDLTSHQ RIHNYEKPYK
110 120 130 140 150
CSKCEKSFWH HLALSGHQRT HAGKKFYTCD ICGKNFGQSS DLLVHQRSHT
160 170 180 190 200
GEKPYLCSEC DKCFSRSTNL IRHRRTHTGE KPFKCLECEK AFSGKSDLIS
210 220 230 240 250
HQRTHTGERP YKCNKCEKSY RHRSAFIVHK RVHTGEKPYK CGACEKCFGQ
260 270 280 290 300
KSDLIVHQRV HTGEKPYKCL ECMRSFTRSA NLIRHQATHT HTFKCLEYEK
310 320 330 340 350
SFNCSSDLIV HQRIHMEEKP HQWSACESGF LLGMDFVAQQ KMRTQTEELH
360 370 380 390 400
YKYTVCDKSF HQSSALLQHQ TVHIGEKPFV CNVSEKGLEL SPPHASEASQ

MS
Length:402
Mass (Da):46,941
Last modified:November 22, 2005 - v2
Checksum:iA9A54EC8884BD27D
GO

Sequence cautioni

The sequence BAB15637 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33H → R in AAQ85127 (PubMed:15555580).Curated1
Sequence conflicti73C → S in BAB15637 (PubMed:14702039).Curated1
Sequence conflicti94N → H in AAQ85127 (PubMed:15555580).Curated1
Sequence conflicti101C → R in AAQ85127 (PubMed:15555580).Curated1
Sequence conflicti373H → R in AAQ85127 (PubMed:15555580).Curated1
Sequence conflicti398A → V in AAQ85127 (PubMed:15555580).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY376736 mRNA Translation: AAQ85127.1
AK027046 mRNA Translation: BAB15637.1 Different initiation.
AK290038 mRNA Translation: BAF82727.1
BC050425 mRNA Translation: AAH50425.2
BC074772 mRNA Translation: AAH74772.1
BC119669 mRNA Translation: AAI19670.1
CCDSiCCDS4617.1
RefSeqiNP_001229726.1, NM_001242797.1
NP_001229727.1, NM_001242798.1
NP_001229728.1, NM_001242799.1
NP_078915.2, NM_024639.4
UniGeneiHs.126280
Hs.530271
Hs.709963

Genome annotation databases

EnsembliENST00000415922; ENSP00000418897; ENSG00000181315
ENST00000456172; ENSP00000478899; ENSG00000181315
ENST00000471278; ENSP00000419728; ENSG00000181315
ENST00000607204; ENSP00000483223; ENSG00000181315
ENST00000622479; ENSP00000482607; ENSG00000181315
GeneIDi79692
KEGGihsa:79692
UCSCiuc003nil.4 human

Similar proteinsi

Entry informationi

Entry nameiZN322_HUMAN
AccessioniPrimary (citable) accession number: Q6U7Q0
Secondary accession number(s): A8K1X3
, Q0VDH6, Q6B0G2, Q86W72, Q9H5I9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health