Q6U7H8 (PI5L1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-phosphate 5-kinase-like protein 1
Short name=PI(4)P 5-kinase-like protein 1
Short name=PtdIns(4)P-5-kinase-like protein 1
EC=2.7.1.68

Alternative name(s):
Phosphatidylinositol phosphate kinase homolog
Short name=PIPKH

Gene names

Name:	Pip5kl1
Synonyms:	Pipkh

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as a scaffold to localize and regulate type I PI4P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3. Ref.1
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Subunit structure	Heterodimerizes with other type I phosphatidylinositol 4-phosphate 5-kinase.
Subcellular location	Cytoplasm. Membrane. Note: Localized to large cytoplasmic vesicular structures. Ref.1
Tissue specificity	Highly expressed in brain and testis, relatively to heart, spleen, lung, liver, skeletal muscle and kidney. Ref.1
Sequence similarities	Contains 1 PIPK domain.
Caution	It is unsure if the enzyme has intrinsic kinase activity.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cell projection Inferred from direct assay Ref.1. Source: MGI cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-phosphatidylinositol-4-phosphate 5-kinase activity Inferred from direct assay Ref.1. Source: MGI ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6U7H8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6U7H8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MATPSLRSHE → MLFVSCASSHQ

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6U7H8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: E → EVGADQRGDKKGKGESLAITLARGLNTPPPRLCSGKIKTRPTE
     308-395: SIQGVSKSKG...LCRWAEVHTE → RTVTPAMLAMALTETTH

Note: No experimental confirmation available.

Isoform 4 (identifier: Q6U7H8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     201-216: KYFIIMQCIFYPTSRI → VSVADRGGSAVGAAGG
     217-395: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Phosphatidylinositol 4-phosphate 5-kinase-like protein 1		PRO_0000285759
Regions
	Domain	37 – 394	358	PIPK
Natural variations
	Alternative sequence	1 – 10	10	MATPSLRSHE → MLFVSCASSHQ in isoform 2.		VSP_024905
	Alternative sequence	10	1	E → EVGADQRGDKKGKGESLAIT LARGLNTPPPRLCSGKIKTR PTE in isoform 3.		VSP_024906
	Alternative sequence	201 – 216	16	KYFII…PTSRI → VSVADRGGSAVGAAGG in isoform 4.		VSP_024907
	Alternative sequence	217 – 395	179	Missing in isoform 4.		VSP_024908
	Alternative sequence	308 – 395	88	SIQGV…EVHTE → RTVTPAMLAMALTETTH in isoform 3.		VSP_024909
Experimental info
	Mutagenesis	155	1	K → R: No change. No change; when associated with A-281. Ref.1
	Mutagenesis	281	1	D → A: No change. No change; when associated with R-155. Ref.1
	Sequence conflict	215 – 218	4	RISE → VLPP in AAH28795. Ref.4
	Sequence conflict	265	1	R → Q in AAH28795. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 22AA0A86324972AB	FASTA	395	45,293
	10 20 30 40 50 60 MATPSLRSHE IPAHSQEAGN KSISSGSRRG LLWHLRARQS RVGLFEVGPG HELHRMTRMM 70 80 90 100 110 120 QEGLWAATQV SKNNPPTGPT TQKDYLEVMT QVHEEGFELG TLAGPAFARL RKSIGLTEED 130 140 150 160 170 180 YQATLGPGDP YLQFFSTSKS KASFFLTHDQ RFFVKTQRRH EVHVLLAHLP RYVEHLQQYP 190 200 210 220 230 240 HSLLARLLGV YSLRVAQGKK KYFIIMQCIF YPTSRISERY DIKGCNISRW VDPAPEGSPL 250 260 270 280 290 300 VLVLKDLNFQ EKTMRLGAQR SWFLRQMELD TAFLREVNVL DYSLLVAIQF LHEDEKGIHH 310 320 330 340 350 360 SVFSTFKSIQ GVSKSKGTGD QNCRMLPDLP NALHILDGPD QRYFLGLVDM TTVYGFRKRL 370 380 390 EHVWKMVRYP GQSVSTVSPA HYARRLCRWA EVHTE
	Isoform 2 [UniParc]. Checksum: 243270F8EC48F3A9	FASTA	396	45,374
	10 20 30 40 50 60 MLFVSCASSH QIPAHSQEAG NKSISSGSRR GLLWHLRARQ SRVGLFEVGP GHELHRMTRM 70 80 90 100 110 120 MQEGLWAATQ VSKNNPPTGP TTQKDYLEVM TQVHEEGFEL GTLAGPAFAR LRKSIGLTEE 130 140 150 160 170 180 DYQATLGPGD PYLQFFSTSK SKASFFLTHD QRFFVKTQRR HEVHVLLAHL PRYVEHLQQY 190 200 210 220 230 240 PHSLLARLLG VYSLRVAQGK KKYFIIMQCI FYPTSRISER YDIKGCNISR WVDPAPEGSP 250 260 270 280 290 300 LVLVLKDLNF QEKTMRLGAQ RSWFLRQMEL DTAFLREVNV LDYSLLVAIQ FLHEDEKGIH 310 320 330 340 350 360 HSVFSTFKSI QGVSKSKGTG DQNCRMLPDL PNALHILDGP DQRYFLGLVD MTTVYGFRKR 370 380 390 LEHVWKMVRY PGQSVSTVSP AHYARRLCRW AEVHTE
	Isoform 3 [UniParc]. Checksum: 9BC2D0BB5AF9FE44	FASTA	366	41,464
	10 20 30 40 50 60 MATPSLRSHE VGADQRGDKK GKGESLAITL ARGLNTPPPR LCSGKIKTRP TEIPAHSQEA 70 80 90 100 110 120 GNKSISSGSR RGLLWHLRAR QSRVGLFEVG PGHELHRMTR MMQEGLWAAT QVSKNNPPTG 130 140 150 160 170 180 PTTQKDYLEV MTQVHEEGFE LGTLAGPAFA RLRKSIGLTE EDYQATLGPG DPYLQFFSTS 190 200 210 220 230 240 KSKASFFLTH DQRFFVKTQR RHEVHVLLAH LPRYVEHLQQ YPHSLLARLL GVYSLRVAQG 250 260 270 280 290 300 KKKYFIIMQC IFYPTSRISE RYDIKGCNIS RWVDPAPEGS PLVLVLKDLN FQEKTMRLGA 310 320 330 340 350 360 QRSWFLRQME LDTAFLREVN VLDYSLLVAI QFLHEDEKGI HHSVFSTFKR TVTPAMLAMA LTETTH
	Isoform 4 [UniParc]. Checksum: D3146CE96D0EEC6F	FASTA	216	23,937
	10 20 30 40 50 60 MATPSLRSHE IPAHSQEAGN KSISSGSRRG LLWHLRARQS RVGLFEVGPG HELHRMTRMM 70 80 90 100 110 120 QEGLWAATQV SKNNPPTGPT TQKDYLEVMT QVHEEGFELG TLAGPAFARL RKSIGLTEED 130 140 150 160 170 180 YQATLGPGDP YLQFFSTSKS KASFFLTHDQ RFFVKTQRRH EVHVLLAHLP RYVEHLQQYP 190 200 210 HSLLARLLGV YSLRVAQGKK VSVADRGGSA VGAAGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a phosphoinositide phosphate kinase homolog." Chang J.D., Field S.J., Rameh L.E., Carpenter C.L., Cantley L.C. J. Biol. Chem. 279:11672-11679(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-155 AND ASP-281. Strain: C57BL/6. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6J. Tissue: Eye and Testis.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4). Strain: C57BL/6J. Tissue: Brain and Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY376879 mRNA. Translation: AAQ92365.1. AK133244 mRNA. Translation: BAE21576.1. AK164998 mRNA. Translation: BAE37997.1. AL928710 Genomic DNA. Translation: CAM18808.1. BC028795 mRNA. Translation: AAH28795.1. BC094346 mRNA. Translation: AAH94346.1. BC117018 mRNA. Translation: AAI17019.1. BC119037 mRNA. Translation: AAI19038.1.
IPI	IPI00381031. IPI00605933. IPI00651813. IPI00845778.
RefSeq	NP_937834.1. NM_198191.2.
UniGene	Mm.78923.
3D structure databases
ProteinModelPortal	Q6U7H8.
SMR	Q6U7H8. Positions 107-289.
ModBase	Search...
PTM databases
PhosphoSite	Q6U7H8.
Proteomic databases
PRIDE	Q6U7H8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000055304; ENSMUSP00000051282; ENSMUSG00000046854. ENSMUST00000100188; ENSMUSP00000097763; ENSMUSG00000046854.
GeneID	227733.
KEGG	mmu:227733.
UCSC	uc008jfv.1. mouse.
Organism-specific databases
CTD	138429.
MGI	MGI:2448520. Pip5kl1.
Phylogenomic databases
eggNOG	COG5253.
GeneTree	ENSGT00690000101870.
HOVERGEN	HBG097351.
InParanoid	Q6U7H8.
KO	K13712.
OMA	PQRSWFL.
OrthoDB	EOG4TB4BW.
Gene expression databases
Bgee	Q6U7H8.
CleanEx	MM_PIP5KL1.
Genevestigator	Q6U7H8.
Family and domain databases
InterPro	IPR023610. PInositol-4-P-5-kinase. IPR002498. PInositol-4-P-5-kinase_core. [Graphical view]
PANTHER	PTHR23086. PTHR23086. 1 hit.
Pfam	PF01504. PIP5K. 1 hit. [Graphical view]
PROSITE	PS51455. PIPK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	378800.
SOURCE	Search...

Entry information

Entry name

PI5L1_MOUSE

Accession

Primary (citable) accession number: Q6U7H8
Secondary accession number(s): A2ASY7 , A2ASY9, Q3TNU0, Q3V0C8, Q52KH3, Q8K345

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program