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Q6U7H8

- PI5L1_MOUSE

UniProt

Q6U7H8 - PI5L1_MOUSE

Protein

Phosphatidylinositol 4-phosphate 5-kinase-like protein 1

Gene

Pip5kl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    May act as a scaffold to localize and regulate type I PI4P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3.1 Publication

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: MGI
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: MGI

    GO - Biological processi

    1. phosphatidylinositol phosphorylation Source: GOC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 4-phosphate 5-kinase-like protein 1 (EC:2.7.1.68)
    Short name:
    PI(4)P 5-kinase-like protein 1
    Short name:
    PtdIns(4)P-5-kinase-like protein 1
    Alternative name(s):
    Phosphatidylinositol phosphate kinase homolog
    Short name:
    PIPKH
    Gene namesi
    Name:Pip5kl1
    Synonyms:Pipkh
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2448520. Pip5kl1.

    Subcellular locationi

    Cytoplasm 1 Publication. Membrane 1 Publication
    Note: Localized to large cytoplasmic vesicular structures.

    GO - Cellular componenti

    1. cell projection Source: MGI
    2. cytoplasm Source: UniProtKB-SubCell
    3. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi155 – 1551K → R: No change. No change; when associated with A-281. 1 Publication
    Mutagenesisi281 – 2811D → A: No change. No change; when associated with R-155. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395Phosphatidylinositol 4-phosphate 5-kinase-like protein 1PRO_0000285759Add
    BLAST

    Proteomic databases

    PRIDEiQ6U7H8.

    PTM databases

    PhosphoSiteiQ6U7H8.

    Expressioni

    Tissue specificityi

    Highly expressed in brain and testis, relatively to heart, spleen, lung, liver, skeletal muscle and kidney.1 Publication

    Gene expression databases

    BgeeiQ6U7H8.
    CleanExiMM_PIP5KL1.
    GenevestigatoriQ6U7H8.

    Interactioni

    Subunit structurei

    Heterodimerizes with other type I phosphatidylinositol 4-phosphate 5-kinase.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6U7H8.
    SMRiQ6U7H8. Positions 107-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 394358PIPKPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PIPK domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5253.
    GeneTreeiENSGT00690000101870.
    HOVERGENiHBG097351.
    InParanoidiQ6U7H8.
    KOiK13712.
    OMAiPQRSWFL.
    OrthoDBiEOG77DJ5R.
    PhylomeDBiQ6U7H8.
    TreeFamiTF354315.

    Family and domain databases

    Gene3Di3.30.800.10. 1 hit.
    3.30.810.10. 1 hit.
    InterProiIPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    [Graphical view]
    PANTHERiPTHR23086. PTHR23086. 1 hit.
    PfamiPF01504. PIP5K. 1 hit.
    [Graphical view]
    PROSITEiPS51455. PIPK. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6U7H8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATPSLRSHE IPAHSQEAGN KSISSGSRRG LLWHLRARQS RVGLFEVGPG    50
    HELHRMTRMM QEGLWAATQV SKNNPPTGPT TQKDYLEVMT QVHEEGFELG 100
    TLAGPAFARL RKSIGLTEED YQATLGPGDP YLQFFSTSKS KASFFLTHDQ 150
    RFFVKTQRRH EVHVLLAHLP RYVEHLQQYP HSLLARLLGV YSLRVAQGKK 200
    KYFIIMQCIF YPTSRISERY DIKGCNISRW VDPAPEGSPL VLVLKDLNFQ 250
    EKTMRLGAQR SWFLRQMELD TAFLREVNVL DYSLLVAIQF LHEDEKGIHH 300
    SVFSTFKSIQ GVSKSKGTGD QNCRMLPDLP NALHILDGPD QRYFLGLVDM 350
    TTVYGFRKRL EHVWKMVRYP GQSVSTVSPA HYARRLCRWA EVHTE 395
    Length:395
    Mass (Da):45,293
    Last modified:July 5, 2004 - v1
    Checksum:i22AA0A86324972AB
    GO
    Isoform 2 (identifier: Q6U7H8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-10: MATPSLRSHE → MLFVSCASSHQ

    Note: No experimental confirmation available.

    Show »
    Length:396
    Mass (Da):45,374
    Checksum:i243270F8EC48F3A9
    GO
    Isoform 3 (identifier: Q6U7H8-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         10-10: E → EVGADQRGDKKGKGESLAITLARGLNTPPPRLCSGKIKTRPTE
         308-395: SIQGVSKSKG...LCRWAEVHTE → RTVTPAMLAMALTETTH

    Note: No experimental confirmation available.

    Show »
    Length:366
    Mass (Da):41,464
    Checksum:i9BC2D0BB5AF9FE44
    GO
    Isoform 4 (identifier: Q6U7H8-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         201-216: KYFIIMQCIFYPTSRI → VSVADRGGSAVGAAGG
         217-395: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:216
    Mass (Da):23,937
    Checksum:iD3146CE96D0EEC6F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti215 – 2184RISE → VLPP in AAH28795. (PubMed:15489334)Curated
    Sequence conflicti265 – 2651R → Q in AAH28795. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1010MATPSLRSHE → MLFVSCASSHQ in isoform 2. 1 PublicationVSP_024905
    Alternative sequencei10 – 101E → EVGADQRGDKKGKGESLAIT LARGLNTPPPRLCSGKIKTR PTE in isoform 3. 1 PublicationVSP_024906
    Alternative sequencei201 – 21616KYFII…PTSRI → VSVADRGGSAVGAAGG in isoform 4. 1 PublicationVSP_024907Add
    BLAST
    Alternative sequencei217 – 395179Missing in isoform 4. 1 PublicationVSP_024908Add
    BLAST
    Alternative sequencei308 – 39588SIQGV…EVHTE → RTVTPAMLAMALTETTH in isoform 3. 1 PublicationVSP_024909Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY376879 mRNA. Translation: AAQ92365.1.
    AK133244 mRNA. Translation: BAE21576.1.
    AK164998 mRNA. Translation: BAE37997.1.
    AL928710 Genomic DNA. Translation: CAM18808.1.
    BC028795 mRNA. Translation: AAH28795.1.
    BC094346 mRNA. Translation: AAH94346.1.
    BC117018 mRNA. Translation: AAI17019.1.
    BC119037 mRNA. Translation: AAI19038.1.
    CCDSiCCDS15920.1. [Q6U7H8-1]
    RefSeqiNP_937834.1. NM_198191.2. [Q6U7H8-1]
    UniGeneiMm.78923.

    Genome annotation databases

    EnsembliENSMUST00000055304; ENSMUSP00000051282; ENSMUSG00000046854. [Q6U7H8-1]
    ENSMUST00000100188; ENSMUSP00000097763; ENSMUSG00000046854. [Q6U7H8-3]
    GeneIDi227733.
    KEGGimmu:227733.
    UCSCiuc008jfv.1. mouse. [Q6U7H8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY376879 mRNA. Translation: AAQ92365.1 .
    AK133244 mRNA. Translation: BAE21576.1 .
    AK164998 mRNA. Translation: BAE37997.1 .
    AL928710 Genomic DNA. Translation: CAM18808.1 .
    BC028795 mRNA. Translation: AAH28795.1 .
    BC094346 mRNA. Translation: AAH94346.1 .
    BC117018 mRNA. Translation: AAI17019.1 .
    BC119037 mRNA. Translation: AAI19038.1 .
    CCDSi CCDS15920.1. [Q6U7H8-1 ]
    RefSeqi NP_937834.1. NM_198191.2. [Q6U7H8-1 ]
    UniGenei Mm.78923.

    3D structure databases

    ProteinModelPortali Q6U7H8.
    SMRi Q6U7H8. Positions 107-289.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6U7H8.

    Proteomic databases

    PRIDEi Q6U7H8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000055304 ; ENSMUSP00000051282 ; ENSMUSG00000046854 . [Q6U7H8-1 ]
    ENSMUST00000100188 ; ENSMUSP00000097763 ; ENSMUSG00000046854 . [Q6U7H8-3 ]
    GeneIDi 227733.
    KEGGi mmu:227733.
    UCSCi uc008jfv.1. mouse. [Q6U7H8-1 ]

    Organism-specific databases

    CTDi 138429.
    MGIi MGI:2448520. Pip5kl1.

    Phylogenomic databases

    eggNOGi COG5253.
    GeneTreei ENSGT00690000101870.
    HOVERGENi HBG097351.
    InParanoidi Q6U7H8.
    KOi K13712.
    OMAi PQRSWFL.
    OrthoDBi EOG77DJ5R.
    PhylomeDBi Q6U7H8.
    TreeFami TF354315.

    Miscellaneous databases

    NextBioi 378800.
    PROi Q6U7H8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6U7H8.
    CleanExi MM_PIP5KL1.
    Genevestigatori Q6U7H8.

    Family and domain databases

    Gene3Di 3.30.800.10. 1 hit.
    3.30.810.10. 1 hit.
    InterProi IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    [Graphical view ]
    PANTHERi PTHR23086. PTHR23086. 1 hit.
    Pfami PF01504. PIP5K. 1 hit.
    [Graphical view ]
    PROSITEi PS51455. PIPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a phosphoinositide phosphate kinase homolog."
      Chang J.D., Field S.J., Rameh L.E., Carpenter C.L., Cantley L.C.
      J. Biol. Chem. 279:11672-11679(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-155 AND ASP-281.
      Strain: C57BL/6.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Strain: C57BL/6J.
      Tissue: Eye and Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
      Strain: C57BL/6J.
      Tissue: Brain and Mammary gland.

    Entry informationi

    Entry nameiPI5L1_MOUSE
    AccessioniPrimary (citable) accession number: Q6U7H8
    Secondary accession number(s): A2ASY7
    , A2ASY9, Q3TNU0, Q3V0C8, Q52KH3, Q8K345
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3