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Q6U7H8 (PI5L1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 4-phosphate 5-kinase-like protein 1

Short name=PI(4)P 5-kinase-like protein 1
Short name=PtdIns(4)P-5-kinase-like protein 1
EC=2.7.1.68
Alternative name(s):
Phosphatidylinositol phosphate kinase homolog
Short name=PIPKH
Gene names
Name:Pip5kl1
Synonyms:Pipkh
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as a scaffold to localize and regulate type I PI4P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3. Ref.1

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Subunit structure

Heterodimerizes with other type I phosphatidylinositol 4-phosphate 5-kinase.

Subcellular location

Cytoplasm. Membrane. Note: Localized to large cytoplasmic vesicular structures. Ref.1

Tissue specificity

Highly expressed in brain and testis, relatively to heart, spleen, lung, liver, skeletal muscle and kidney. Ref.1

Sequence similarities

Contains 1 PIPK domain.

Caution

It is unsure if the enzyme has intrinsic kinase activity.

Ontologies

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6U7H8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6U7H8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MATPSLRSHE → MLFVSCASSHQ
Note: No experimental confirmation available.
Isoform 3 (identifier: Q6U7H8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: E → EVGADQRGDKKGKGESLAITLARGLNTPPPRLCSGKIKTRPTE
     308-395: SIQGVSKSKG...LCRWAEVHTE → RTVTPAMLAMALTETTH
Note: No experimental confirmation available.
Isoform 4 (identifier: Q6U7H8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     201-216: KYFIIMQCIFYPTSRI → VSVADRGGSAVGAAGG
     217-395: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395Phosphatidylinositol 4-phosphate 5-kinase-like protein 1
PRO_0000285759

Regions

Domain37 – 394358PIPK

Natural variations

Alternative sequence1 – 1010MATPSLRSHE → MLFVSCASSHQ in isoform 2.
VSP_024905
Alternative sequence101E → EVGADQRGDKKGKGESLAIT LARGLNTPPPRLCSGKIKTR PTE in isoform 3.
VSP_024906
Alternative sequence201 – 21616KYFII…PTSRI → VSVADRGGSAVGAAGG in isoform 4.
VSP_024907
Alternative sequence217 – 395179Missing in isoform 4.
VSP_024908
Alternative sequence308 – 39588SIQGV…EVHTE → RTVTPAMLAMALTETTH in isoform 3.
VSP_024909

Experimental info

Mutagenesis1551K → R: No change. No change; when associated with A-281. Ref.1
Mutagenesis2811D → A: No change. No change; when associated with R-155. Ref.1
Sequence conflict215 – 2184RISE → VLPP in AAH28795. Ref.4
Sequence conflict2651R → Q in AAH28795. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 22AA0A86324972AB

FASTA39545,293
        10         20         30         40         50         60 
MATPSLRSHE IPAHSQEAGN KSISSGSRRG LLWHLRARQS RVGLFEVGPG HELHRMTRMM 

        70         80         90        100        110        120 
QEGLWAATQV SKNNPPTGPT TQKDYLEVMT QVHEEGFELG TLAGPAFARL RKSIGLTEED 

       130        140        150        160        170        180 
YQATLGPGDP YLQFFSTSKS KASFFLTHDQ RFFVKTQRRH EVHVLLAHLP RYVEHLQQYP 

       190        200        210        220        230        240 
HSLLARLLGV YSLRVAQGKK KYFIIMQCIF YPTSRISERY DIKGCNISRW VDPAPEGSPL 

       250        260        270        280        290        300 
VLVLKDLNFQ EKTMRLGAQR SWFLRQMELD TAFLREVNVL DYSLLVAIQF LHEDEKGIHH 

       310        320        330        340        350        360 
SVFSTFKSIQ GVSKSKGTGD QNCRMLPDLP NALHILDGPD QRYFLGLVDM TTVYGFRKRL 

       370        380        390 
EHVWKMVRYP GQSVSTVSPA HYARRLCRWA EVHTE 

« Hide

Isoform 2 [UniParc].

Checksum: 243270F8EC48F3A9
Show »

FASTA39645,374
Isoform 3 [UniParc].

Checksum: 9BC2D0BB5AF9FE44
Show »

FASTA36641,464
Isoform 4 [UniParc].

Checksum: D3146CE96D0EEC6F
Show »

FASTA21623,937

References

« Hide 'large scale' references
[1]"Identification and characterization of a phosphoinositide phosphate kinase homolog."
Chang J.D., Field S.J., Rameh L.E., Carpenter C.L., Cantley L.C.
J. Biol. Chem. 279:11672-11679(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-155 AND ASP-281.
Strain: C57BL/6.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Strain: C57BL/6J.
Tissue: Eye and Testis.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
Strain: C57BL/6J.
Tissue: Brain and Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY376879 mRNA. Translation: AAQ92365.1.
AK133244 mRNA. Translation: BAE21576.1.
AK164998 mRNA. Translation: BAE37997.1.
AL928710 Genomic DNA. Translation: CAM18808.1.
BC028795 mRNA. Translation: AAH28795.1.
BC094346 mRNA. Translation: AAH94346.1.
BC117018 mRNA. Translation: AAI17019.1.
BC119037 mRNA. Translation: AAI19038.1.
CCDSCCDS15920.1. [Q6U7H8-1]
RefSeqNP_937834.1. NM_198191.2. [Q6U7H8-1]
UniGeneMm.78923.

3D structure databases

ProteinModelPortalQ6U7H8.
SMRQ6U7H8. Positions 107-289.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ6U7H8.

Proteomic databases

PRIDEQ6U7H8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000055304; ENSMUSP00000051282; ENSMUSG00000046854. [Q6U7H8-1]
ENSMUST00000100188; ENSMUSP00000097763; ENSMUSG00000046854. [Q6U7H8-3]
GeneID227733.
KEGGmmu:227733.
UCSCuc008jfv.1. mouse. [Q6U7H8-1]

Organism-specific databases

CTD138429.
MGIMGI:2448520. Pip5kl1.

Phylogenomic databases

eggNOGCOG5253.
GeneTreeENSGT00690000101870.
HOVERGENHBG097351.
InParanoidQ6U7H8.
KOK13712.
OMAPQRSWFL.
OrthoDBEOG77DJ5R.
PhylomeDBQ6U7H8.
TreeFamTF354315.

Gene expression databases

BgeeQ6U7H8.
CleanExMM_PIP5KL1.
GenevestigatorQ6U7H8.

Family and domain databases

Gene3D3.30.800.10. 1 hit.
3.30.810.10. 1 hit.
InterProIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
[Graphical view]
PANTHERPTHR23086. PTHR23086. 1 hit.
PfamPF01504. PIP5K. 1 hit.
[Graphical view]
PROSITEPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio378800.
PROQ6U7H8.
SOURCESearch...

Entry information

Entry namePI5L1_MOUSE
AccessionPrimary (citable) accession number: Q6U7H8
Secondary accession number(s): A2ASY7 expand/collapse secondary AC list , A2ASY9, Q3TNU0, Q3V0C8, Q52KH3, Q8K345
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot