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Protein

Juvenile hormone epoxide hydrolase

Gene

JHEH

Organism
Bombyx mori (Silk moth)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes juvenile hormone hydrolysis. Degrades juvenile hormone III (JH III) about 3 times and 5 times slower than juvenile hormone I (JH I) and II (JH II), respectively. Degrades cis-stilbene oxide and trans-stilbene oxide about 18 and 43 times slower than JH III, respectively.1 Publication

Catalytic activityi

Cis-stilbene oxide + H2O = (+)-(1R,2R)-1,2-diphenylethane-1,2-diol.1 Publication

Kineticsi

  1. KM=0.52 µM for juvenile hormone III1 Publication
  1. Vmax=122 nmol/min/mg enzyme with juvenile hormone I as substrate1 Publication
  2. Vmax=223 nmol/min/mg enzyme with juvenile hormone II as substrate1 Publication
  3. Vmax=46 nmol/min/mg enzyme with juvenile hormone III as substrate1 Publication

pH dependencei

Optimum pH is 7.5-8.0. Activity decreases rapidly below pH 7.0 and above pH 9.0.1 Publication

Temperature dependencei

Activity increases as temperature increases from 0 to 37 degrees Celsius.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Protein family/group databases

ESTHERibommo-q6u6j0. Epoxide_hydrolase.
MEROPSiS33.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Juvenile hormone epoxide hydrolase1 PublicationImported (EC:3.3.2.9)
Short name:
bommo-JHEH1 Publication
Gene namesi
Name:JHEHImported
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
Proteomesi
  • UP000005204 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei4 – 24Helical; Signal-anchorSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003895211 – 461Juvenile hormone epoxide hydrolaseAdd BLAST461

Expressioni

Tissue specificityi

Expressed in fat body, foregut and midgut but not in brain, subesophageal ganglia or silk gland of larvae on day 1 of fifth instar.1 Publication

Developmental stagei

Expressed in fourth and fifth instar larvae. Initial levels decline until day 2 of fourth instar but increase sharply on day 3. Levels do not significantly change on days 0 and 1 of fifth instar, increase on day 2 before falling again on days 3-4 to the level seen at day 2 of the fourth instar. Expression then remains low until the end of the instar.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7091.BGIBMGA013930-TA.

Structurei

Secondary structure

1461
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi42 – 46Combined sources5
Beta strandi53 – 55Combined sources3
Helixi61 – 72Combined sources12
Turni85 – 88Combined sources4
Helixi92 – 94Combined sources3
Helixi95 – 104Combined sources10
Helixi108 – 115Combined sources8
Beta strandi120 – 125Combined sources6
Beta strandi128 – 135Combined sources8
Beta strandi144 – 151Combined sources8
Helixi158 – 164Combined sources7
Helixi165 – 168Combined sources4
Beta strandi176 – 184Combined sources9
Beta strandi196 – 198Combined sources3
Helixi202 – 216Combined sources15
Beta strandi219 – 226Combined sources8
Helixi228 – 239Combined sources12
Turni241 – 243Combined sources3
Beta strandi244 – 251Combined sources8
Helixi257 – 268Combined sources12
Helixi270 – 272Combined sources3
Turni276 – 278Combined sources3
Helixi279 – 281Combined sources3
Helixi285 – 295Combined sources11
Helixi297 – 304Combined sources8
Helixi306 – 315Combined sources10
Helixi317 – 330Combined sources14
Helixi334 – 338Combined sources5
Beta strandi339 – 341Combined sources3
Turni342 – 345Combined sources4
Helixi350 – 361Combined sources12
Turni362 – 364Combined sources3
Helixi366 – 376Combined sources11
Helixi379 – 382Combined sources4
Turni386 – 388Combined sources3
Beta strandi395 – 399Combined sources5
Helixi409 – 415Combined sources7
Beta strandi419 – 424Combined sources6
Helixi432 – 435Combined sources4
Helixi437 – 454Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QLAX-ray2.30A/B23-461[»]
ProteinModelPortaliQ6U6J0.
SMRiQ6U6J0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S33 family.Sequence analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2565. Eukaryota.
COG0596. LUCA.
InParanoidiQ6U6J0.
KOiK10719.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000639. Epox_hydrolase-like.
IPR010497. Epoxide_hydro_N.
IPR016292. Epoxide_hydrolase.
[Graphical view]
PfamiPF06441. EHN. 1 hit.
[Graphical view]
PIRSFiPIRSF001112. Epoxide_hydrolase. 1 hit.
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6U6J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLLFIALP LLVLASIPLY LLVLKSPPPM PKLDLEEWWG PPELKQKQDT
60 70 80 90 100
SIKPFEITFS ETMVKELKER IKKRRPFAPP LEGVGFKYGF NSKQLDSWLK
110 120 130 140 150
YWAEEYPFAE RQKFLNQYPH FKTNIQGLNI HFMRITPKVP KDVEIVPLLL
160 170 180 190 200
LHGWPGSVRE FYEAIPHLTA VSRDRNFALE IIAPSLPGYG FSDAAVRPGL
210 220 230 240 250
AAAEVAVIFK NLMARLGYKQ YYVQGGDWGA LIGSAMATSF PKEIIGFHSY
260 270 280 290 300
MALTLSPAAT FLEFVGALFP SLIVEPELAN RLYPLSEKYS TLLEELGYMH
310 320 330 340 350
IQATKPDTVG IGLTDSPAGL LAYILEKFST WTNPDLRSKE DGGLSYRWTK
360 370 380 390 400
DQLIDNLMLY WSTKSIVTSM RLYAESFSSR HFDLKLDEIQ VQVPTWVLQA
410 420 430 440 450
KHELAYQPPC ILKLKYTKLV NASVIEDGGH FLAFELPEIF AKDVLKAIGE
460
FRKLKNVKTE L
Length:461
Mass (Da):52,441
Last modified:July 5, 2004 - v1
Checksum:i21A6EE19AD54CA57
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 8FIA → LIV in BAF81491 (Ref. 2) Curated3
Sequence conflicti173R → K in BAF81491 (Ref. 2) Curated1
Sequence conflicti239S → F in BAF81491 (Ref. 2) Curated1
Sequence conflicti250Y → N in BAF81491 (Ref. 2) Curated1
Sequence conflicti417T → P in BAF81491 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY377854 mRNA. Translation: AAQ87024.1.
AB362775 mRNA. Translation: BAF81491.1.
RefSeqiNP_001037201.1. NM_001043736.1.
UniGeneiBmo.321.

Genome annotation databases

GeneIDi692686.
KEGGibmor:692686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY377854 mRNA. Translation: AAQ87024.1.
AB362775 mRNA. Translation: BAF81491.1.
RefSeqiNP_001037201.1. NM_001043736.1.
UniGeneiBmo.321.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QLAX-ray2.30A/B23-461[»]
ProteinModelPortaliQ6U6J0.
SMRiQ6U6J0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7091.BGIBMGA013930-TA.

Protein family/group databases

ESTHERibommo-q6u6j0. Epoxide_hydrolase.
MEROPSiS33.971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi692686.
KEGGibmor:692686.

Organism-specific databases

CTDi37181.

Phylogenomic databases

eggNOGiKOG2565. Eukaryota.
COG0596. LUCA.
InParanoidiQ6U6J0.
KOiK10719.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000639. Epox_hydrolase-like.
IPR010497. Epoxide_hydro_N.
IPR016292. Epoxide_hydrolase.
[Graphical view]
PfamiPF06441. EHN. 1 hit.
[Graphical view]
PIRSFiPIRSF001112. Epoxide_hydrolase. 1 hit.
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHYEP_BOMMO
AccessioniPrimary (citable) accession number: Q6U6J0
Secondary accession number(s): A8CGI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.