Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6U6J0

- HYEP_BOMMO

UniProt

Q6U6J0 - HYEP_BOMMO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Juvenile hormone epoxide hydrolase

Gene

JHEH

Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes juvenile hormone hydrolysis. Degrades juvenile hormone III (JH III) about 3 times and 5 times slower than juvenile hormone I (JH I) and II (JH II), respectively. Degrades cis-stilbene oxide and trans-stilbene oxide about 18 and 43 times slower than JH III, respectively.1 Publication

Catalytic activityi

Cis-stilbene oxide + H2O = (+)-(1R,2R)-1,2-diphenylethane-1,2-diol.1 Publication

Kineticsi

  1. KM=0.52 µM for juvenile hormone III1 Publication

Vmax=122 nmol/min/mg enzyme with juvenile hormone I as substrate1 Publication

Vmax=223 nmol/min/mg enzyme with juvenile hormone II as substrate1 Publication

Vmax=46 nmol/min/mg enzyme with juvenile hormone III as substrate1 Publication

pH dependencei

Optimum pH is 7.5-8.0. Activity decreases rapidly below pH 7.0 and above pH 9.0.1 Publication

Temperature dependencei

Activity increases as temperature increases from 0 to 37 degrees Celsius.1 Publication

GO - Molecular functioni

  1. cis-stilbene-oxide hydrolase activity Source: UniProtKB-EC

GO - Biological processi

  1. aromatic compound catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Juvenile hormone epoxide hydrolaseImported1 Publication (EC:3.3.2.9)
Short name:
bommo-JHEH1 Publication
Gene namesi
Name:JHEHImported
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
ProteomesiUP000005204: Unplaced

Subcellular locationi

Microsome membrane 1 PublicationCurated; Single-pass type II membrane protein 1 Publication. Endoplasmic reticulum membrane 1 PublicationCurated; Single-pass type II membrane protein 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Juvenile hormone epoxide hydrolasePRO_0000389521Add
BLAST

Expressioni

Tissue specificityi

Expressed in fat body, foregut and midgut but not in brain, subesophageal ganglia or silk gland of larvae on day 1 of fifth instar.1 Publication

Developmental stagei

Expressed in fourth and fifth instar larvae. Initial levels decline until day 2 of fourth instar but increase sharply on day 3. Levels do not significantly change on days 0 and 1 of fifth instar, increase on day 2 before falling again on days 3-4 to the level seen at day 2 of the fourth instar. Expression then remains low until the end of the instar.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6U6J0.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421Helical; Signal-anchorSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S33 family.Sequence Analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ6U6J0.
KOiK10719.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR010497. Epoxide_hydro_N.
IPR016292. Epoxide_hydrolase.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
PF06441. EHN. 1 hit.
[Graphical view]
PIRSFiPIRSF001112. Epoxide_hydrolase. 1 hit.
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6U6J0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRLLFIALP LLVLASIPLY LLVLKSPPPM PKLDLEEWWG PPELKQKQDT
60 70 80 90 100
SIKPFEITFS ETMVKELKER IKKRRPFAPP LEGVGFKYGF NSKQLDSWLK
110 120 130 140 150
YWAEEYPFAE RQKFLNQYPH FKTNIQGLNI HFMRITPKVP KDVEIVPLLL
160 170 180 190 200
LHGWPGSVRE FYEAIPHLTA VSRDRNFALE IIAPSLPGYG FSDAAVRPGL
210 220 230 240 250
AAAEVAVIFK NLMARLGYKQ YYVQGGDWGA LIGSAMATSF PKEIIGFHSY
260 270 280 290 300
MALTLSPAAT FLEFVGALFP SLIVEPELAN RLYPLSEKYS TLLEELGYMH
310 320 330 340 350
IQATKPDTVG IGLTDSPAGL LAYILEKFST WTNPDLRSKE DGGLSYRWTK
360 370 380 390 400
DQLIDNLMLY WSTKSIVTSM RLYAESFSSR HFDLKLDEIQ VQVPTWVLQA
410 420 430 440 450
KHELAYQPPC ILKLKYTKLV NASVIEDGGH FLAFELPEIF AKDVLKAIGE
460
FRKLKNVKTE L
Length:461
Mass (Da):52,441
Last modified:July 5, 2004 - v1
Checksum:i21A6EE19AD54CA57
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 83FIA → LIV in BAF81491. 1 PublicationCurated
Sequence conflicti173 – 1731R → K in BAF81491. 1 PublicationCurated
Sequence conflicti239 – 2391S → F in BAF81491. 1 PublicationCurated
Sequence conflicti250 – 2501Y → N in BAF81491. 1 PublicationCurated
Sequence conflicti417 – 4171T → P in BAF81491. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY377854 mRNA. Translation: AAQ87024.1.
AB362775 mRNA. Translation: BAF81491.1.
RefSeqiNP_001037201.1. NM_001043736.1.
UniGeneiBmo.321.

Genome annotation databases

GeneIDi692686.
KEGGibmor:692686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY377854 mRNA. Translation: AAQ87024.1 .
AB362775 mRNA. Translation: BAF81491.1 .
RefSeqi NP_001037201.1. NM_001043736.1.
UniGenei Bmo.321.

3D structure databases

ProteinModelPortali Q6U6J0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 692686.
KEGGi bmor:692686.

Organism-specific databases

CTDi 37181.

Phylogenomic databases

InParanoidi Q6U6J0.
KOi K10719.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR010497. Epoxide_hydro_N.
IPR016292. Epoxide_hydrolase.
[Graphical view ]
Pfami PF00561. Abhydrolase_1. 1 hit.
PF06441. EHN. 1 hit.
[Graphical view ]
PIRSFi PIRSF001112. Epoxide_hydrolase. 1 hit.
PRINTSi PR00412. EPOXHYDRLASE.
SUPFAMi SSF53474. SSF53474. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular and biochemical characterization of juvenile hormone epoxide hydrolase from the silkworm, Bombyx mori."
    Zhang Q.R., Xu W.H., Chen F.S., Li S.
    Insect Biochem. Mol. Biol. 35:153-164(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Jingsong X Haoyue1 Publication.
    Tissue: Larval fat body1 Publication.
  2. "Characterization of juvenile hormone epoxide hydrolase and its related genes in larval development of silkworm, Bombyx mori."
    Seino A., Ogura T., Tsubota T., Shimomura M., Tan A., Mita K., Shinoda T., Nakagawa Y., Shiotsuki T.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiHYEP_BOMMO
AccessioniPrimary (citable) accession number: Q6U6J0
Secondary accession number(s): A8CGI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3