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Q6U6J0

- HYEP_BOMMO

UniProt

Q6U6J0 - HYEP_BOMMO

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Protein

Juvenile hormone epoxide hydrolase

Gene
JHEH
Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes juvenile hormone hydrolysis. Degrades juvenile hormone III (JH III) about 3 times and 5 times slower than juvenile hormone I (JH I) and II (JH II), respectively. Degrades cis-stilbene oxide and trans-stilbene oxide about 18 and 43 times slower than JH III, respectively.1 Publication

Catalytic activityi

Cis-stilbene oxide + H2O = (+)-(1R,2R)-1,2-diphenylethane-1,2-diol.1 Publication

Kineticsi

  1. KM=0.52 µM for juvenile hormone III1 Publication

Vmax=122 nmol/min/mg enzyme with juvenile hormone I as substrate1 Publication

Vmax=223 nmol/min/mg enzyme with juvenile hormone II as substrate

Vmax=46 nmol/min/mg enzyme with juvenile hormone III as substrate

pH dependencei

Optimum pH is 7.5-8.0. Activity decreases rapidly below pH 7.0 and above pH 9.0.1 Publication

Temperature dependencei

Activity increases as temperature increases from 0 to 37 degrees Celsius.1 Publication

GO - Molecular functioni

  1. cis-stilbene-oxide hydrolase activity Source: UniProtKB-EC

GO - Biological processi

  1. aromatic compound catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Juvenile hormone epoxide hydrolase (EC:3.3.2.9)
Short name:
bommo-JHEH
Gene namesi
Name:JHEH
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
ProteomesiUP000005204: Unplaced

Subcellular locationi

Microsome membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421Helical; Signal-anchor; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Juvenile hormone epoxide hydrolasePRO_0000389521Add
BLAST

Expressioni

Tissue specificityi

Expressed in fat body, foregut and midgut but not in brain, subesophageal ganglia or silk gland of larvae on day 1 of fifth instar.1 Publication

Developmental stagei

Expressed in fourth and fifth instar larvae. Initial levels decline until day 2 of fourth instar but increase sharply on day 3. Levels do not significantly change on days 0 and 1 of fifth instar, increase on day 2 before falling again on days 3-4 to the level seen at day 2 of the fourth instar. Expression then remains low until the end of the instar.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6U6J0.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S33 family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK10719.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR010497. Epoxide_hydro_N.
IPR016292. Epoxide_hydrolase.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
PF06441. EHN. 1 hit.
[Graphical view]
PIRSFiPIRSF001112. Epoxide_hydrolase. 1 hit.
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6U6J0-1 [UniParc]FASTAAdd to Basket

« Hide

MSRLLFIALP LLVLASIPLY LLVLKSPPPM PKLDLEEWWG PPELKQKQDT    50
SIKPFEITFS ETMVKELKER IKKRRPFAPP LEGVGFKYGF NSKQLDSWLK 100
YWAEEYPFAE RQKFLNQYPH FKTNIQGLNI HFMRITPKVP KDVEIVPLLL 150
LHGWPGSVRE FYEAIPHLTA VSRDRNFALE IIAPSLPGYG FSDAAVRPGL 200
AAAEVAVIFK NLMARLGYKQ YYVQGGDWGA LIGSAMATSF PKEIIGFHSY 250
MALTLSPAAT FLEFVGALFP SLIVEPELAN RLYPLSEKYS TLLEELGYMH 300
IQATKPDTVG IGLTDSPAGL LAYILEKFST WTNPDLRSKE DGGLSYRWTK 350
DQLIDNLMLY WSTKSIVTSM RLYAESFSSR HFDLKLDEIQ VQVPTWVLQA 400
KHELAYQPPC ILKLKYTKLV NASVIEDGGH FLAFELPEIF AKDVLKAIGE 450
FRKLKNVKTE L 461
Length:461
Mass (Da):52,441
Last modified:July 5, 2004 - v1
Checksum:i21A6EE19AD54CA57
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 83FIA → LIV in BAF81491. 1 Publication
Sequence conflicti173 – 1731R → K in BAF81491. 1 Publication
Sequence conflicti239 – 2391S → F in BAF81491. 1 Publication
Sequence conflicti250 – 2501Y → N in BAF81491. 1 Publication
Sequence conflicti417 – 4171T → P in BAF81491. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY377854 mRNA. Translation: AAQ87024.1.
AB362775 mRNA. Translation: BAF81491.1.
RefSeqiNP_001037201.1. NM_001043736.1.
UniGeneiBmo.321.

Genome annotation databases

GeneIDi692686.
KEGGibmor:692686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY377854 mRNA. Translation: AAQ87024.1 .
AB362775 mRNA. Translation: BAF81491.1 .
RefSeqi NP_001037201.1. NM_001043736.1.
UniGenei Bmo.321.

3D structure databases

ProteinModelPortali Q6U6J0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 692686.
KEGGi bmor:692686.

Organism-specific databases

CTDi 37181.

Phylogenomic databases

KOi K10719.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR010497. Epoxide_hydro_N.
IPR016292. Epoxide_hydrolase.
[Graphical view ]
Pfami PF00561. Abhydrolase_1. 1 hit.
PF06441. EHN. 1 hit.
[Graphical view ]
PIRSFi PIRSF001112. Epoxide_hydrolase. 1 hit.
PRINTSi PR00412. EPOXHYDRLASE.
SUPFAMi SSF53474. SSF53474. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular and biochemical characterization of juvenile hormone epoxide hydrolase from the silkworm, Bombyx mori."
    Zhang Q.R., Xu W.H., Chen F.S., Li S.
    Insect Biochem. Mol. Biol. 35:153-164(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Jingsong X Haoyue.
    Tissue: Larval fat body.
  2. "Characterization of juvenile hormone epoxide hydrolase and its related genes in larval development of silkworm, Bombyx mori."
    Seino A., Ogura T., Tsubota T., Shimomura M., Tan A., Mita K., Shinoda T., Nakagawa Y., Shiotsuki T.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiHYEP_BOMMO
AccessioniPrimary (citable) accession number: Q6U6J0
Secondary accession number(s): A8CGI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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