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Q6U6J0

- HYEP_BOMMO

UniProt

Q6U6J0 - HYEP_BOMMO

Protein

Juvenile hormone epoxide hydrolase

Gene

JHEH

Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes juvenile hormone hydrolysis. Degrades juvenile hormone III (JH III) about 3 times and 5 times slower than juvenile hormone I (JH I) and II (JH II), respectively. Degrades cis-stilbene oxide and trans-stilbene oxide about 18 and 43 times slower than JH III, respectively.1 Publication

    Catalytic activityi

    Cis-stilbene oxide + H2O = (+)-(1R,2R)-1,2-diphenylethane-1,2-diol.1 Publication

    Kineticsi

    1. KM=0.52 µM for juvenile hormone III1 Publication

    Vmax=122 nmol/min/mg enzyme with juvenile hormone I as substrate1 Publication

    Vmax=223 nmol/min/mg enzyme with juvenile hormone II as substrate1 Publication

    Vmax=46 nmol/min/mg enzyme with juvenile hormone III as substrate1 Publication

    pH dependencei

    Optimum pH is 7.5-8.0. Activity decreases rapidly below pH 7.0 and above pH 9.0.1 Publication

    Temperature dependencei

    Activity increases as temperature increases from 0 to 37 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. cis-stilbene-oxide hydrolase activity Source: UniProtKB-EC

    GO - Biological processi

    1. aromatic compound catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Juvenile hormone epoxide hydrolaseImported1 Publication (EC:3.3.2.9)
    Short name:
    bommo-JHEH1 Publication
    Gene namesi
    Name:JHEHImported
    OrganismiBombyx mori (Silk moth)
    Taxonomic identifieri7091 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
    ProteomesiUP000005204: Unplaced

    Subcellular locationi

    Microsome membrane 1 PublicationCurated; Single-pass type II membrane protein 1 Publication. Endoplasmic reticulum membrane 1 PublicationCurated; Single-pass type II membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 461461Juvenile hormone epoxide hydrolasePRO_0000389521Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in fat body, foregut and midgut but not in brain, subesophageal ganglia or silk gland of larvae on day 1 of fifth instar.1 Publication

    Developmental stagei

    Expressed in fourth and fifth instar larvae. Initial levels decline until day 2 of fourth instar but increase sharply on day 3. Levels do not significantly change on days 0 and 1 of fifth instar, increase on day 2 before falling again on days 3-4 to the level seen at day 2 of the fourth instar. Expression then remains low until the end of the instar.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ6U6J0.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2421Helical; Signal-anchorSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S33 family.Sequence Analysis

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK10719.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR010497. Epoxide_hydro_N.
    IPR016292. Epoxide_hydrolase.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    PF06441. EHN. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001112. Epoxide_hydrolase. 1 hit.
    PRINTSiPR00412. EPOXHYDRLASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6U6J0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRLLFIALP LLVLASIPLY LLVLKSPPPM PKLDLEEWWG PPELKQKQDT    50
    SIKPFEITFS ETMVKELKER IKKRRPFAPP LEGVGFKYGF NSKQLDSWLK 100
    YWAEEYPFAE RQKFLNQYPH FKTNIQGLNI HFMRITPKVP KDVEIVPLLL 150
    LHGWPGSVRE FYEAIPHLTA VSRDRNFALE IIAPSLPGYG FSDAAVRPGL 200
    AAAEVAVIFK NLMARLGYKQ YYVQGGDWGA LIGSAMATSF PKEIIGFHSY 250
    MALTLSPAAT FLEFVGALFP SLIVEPELAN RLYPLSEKYS TLLEELGYMH 300
    IQATKPDTVG IGLTDSPAGL LAYILEKFST WTNPDLRSKE DGGLSYRWTK 350
    DQLIDNLMLY WSTKSIVTSM RLYAESFSSR HFDLKLDEIQ VQVPTWVLQA 400
    KHELAYQPPC ILKLKYTKLV NASVIEDGGH FLAFELPEIF AKDVLKAIGE 450
    FRKLKNVKTE L 461
    Length:461
    Mass (Da):52,441
    Last modified:July 5, 2004 - v1
    Checksum:i21A6EE19AD54CA57
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 83FIA → LIV in BAF81491. 1 PublicationCurated
    Sequence conflicti173 – 1731R → K in BAF81491. 1 PublicationCurated
    Sequence conflicti239 – 2391S → F in BAF81491. 1 PublicationCurated
    Sequence conflicti250 – 2501Y → N in BAF81491. 1 PublicationCurated
    Sequence conflicti417 – 4171T → P in BAF81491. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY377854 mRNA. Translation: AAQ87024.1.
    AB362775 mRNA. Translation: BAF81491.1.
    RefSeqiNP_001037201.1. NM_001043736.1.
    UniGeneiBmo.321.

    Genome annotation databases

    GeneIDi692686.
    KEGGibmor:692686.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY377854 mRNA. Translation: AAQ87024.1 .
    AB362775 mRNA. Translation: BAF81491.1 .
    RefSeqi NP_001037201.1. NM_001043736.1.
    UniGenei Bmo.321.

    3D structure databases

    ProteinModelPortali Q6U6J0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 692686.
    KEGGi bmor:692686.

    Organism-specific databases

    CTDi 37181.

    Phylogenomic databases

    KOi K10719.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR010497. Epoxide_hydro_N.
    IPR016292. Epoxide_hydrolase.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    PF06441. EHN. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001112. Epoxide_hydrolase. 1 hit.
    PRINTSi PR00412. EPOXHYDRLASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular and biochemical characterization of juvenile hormone epoxide hydrolase from the silkworm, Bombyx mori."
      Zhang Q.R., Xu W.H., Chen F.S., Li S.
      Insect Biochem. Mol. Biol. 35:153-164(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Jingsong X Haoyue1 Publication.
      Tissue: Larval fat body1 Publication.
    2. "Characterization of juvenile hormone epoxide hydrolase and its related genes in larval development of silkworm, Bombyx mori."
      Seino A., Ogura T., Tsubota T., Shimomura M., Tan A., Mita K., Shinoda T., Nakagawa Y., Shiotsuki T.
      Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiHYEP_BOMMO
    AccessioniPrimary (citable) accession number: Q6U6J0
    Secondary accession number(s): A8CGI8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3