Q6TV02 (LAP_YMTV5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase LAP
EC=6.3.2.-

Alternative name(s):
Leukemia associated protein
Short name=LAP

Gene names

Name:	LAP
ORF Names:	5L

Organism

Yaba monkey tumor virus (strain VR587) (YMTV) [Reference proteome]

Taxonomic identifier

928314 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Yatapoxvirus › Yaba monkey tumor virus

Virus host

Erythrocebus patas (Red guenon) (Cercopithecus patas) [TaxID: 9538]
Homo sapiens (Human) [TaxID: 9606]
Macaca (macaques) [TaxID: 9539]
Papio hamadryas (Hamadryas baboon) [TaxID: 9557]

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	E3 ubiquitin-protein ligase which promotes ubiquitination and subsequent degradation of host MHC-I and CD4 molecules, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell. Binds target molecules through transmembrane interaction. The result of this ubiquitination is the enhancement of the endocytosis of the target chain and the delivery to the lysosome, where it is proteolytically destroyed By similarity.
Subcellular location	Host membrane; Multi-pass membrane protein Potential. Host Golgi apparatus › host trans-Golgi network membrane. Host early endosome membrane By similarity.
Domain	The RING-CH-type zinc finger domain is required for E3 ligase activity By similarity.
Sequence similarities	Belongs to the poxviridae LAP protein family. Contains 1 RING-CH-type zinc finger.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host MHC class I molecule presentation by virus Inhibition of host adaptive immune response by virus Modulation of host ubiquitin pathway by viral E3 ligase Modulation of host ubiquitin pathway by virus Ubl conjugation pathway Viral immunoevasion
Cellular component	Host Golgi apparatus Host endosome Host membrane Membrane
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	modulation by virus of host protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell host cell endosome Inferred from electronic annotation. Source: UniProtKB-KW host cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	E3 ubiquitin-protein ligase LAP		PRO_0000396005
Regions
	Topological domain	1 – 73	73	Cytoplasmic Potential
	Transmembrane	74 – 94	21	Helical; Potential
	Topological domain	95 – 116	22	Lumenal Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Topological domain	138 – 156	19	Cytoplasmic Potential
	Zinc finger	1 – 55	55	RING-CH-type

Sequences

	Sequence		Length	Mass (Da)
	Q6TV02 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6D18FBC3965A19E6	FASTA	156	18,290
	10 20 30 40 50 60 MSNICWICND TCDERNNFCI CSEEYKIVHL KCMQSWINYS KKVECDLCKN KYNIKKSYHY 70 80 90 100 110 120 FSRWKWCFSD KKTVLSKILF IFFAVGFIFI TTSMSSNVAS LVTRIDDTFF DVVFLTVYIS 130 140 150 MILVTVCLCV FVLALAVDFL LDAKEKNSFL TIKEIV

References

[1]

"Complete genomic sequence and comparative analysis of the tumorigenic poxvirus Yaba monkey tumor virus." Brunetti C.R., Amano H., Ueda Y., Qin J., Miyamura T., Suzuki T., Li X., Barrett J.W., McFadden G. J. Virol. 77:13335-13347(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY386371 Genomic DNA. Translation: AAR07367.1.
RefSeq	NP_938266.1. NC_005179.1.
3D structure databases
ProteinModelPortal	Q6TV02.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2943673.
Phylogenomic databases
ProtClustDB	PHA2862.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR011016. Znf_RING-CH. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF12906. RINGv. 1 hit. [Graphical view]
SMART	SM00744. RINGv. 1 hit. [Graphical view]
PROSITE	PS51292. ZF_RING_CH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAP_YMTV5

Accession

Primary (citable) accession number: Q6TV02

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program