Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endoribonuclease Dicer

Gene

DICER1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes (By similarity).By similarity

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.Curated

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1316Magnesium or manganese 1By similarity1
Metal bindingi1396Magnesium or manganese 1By similarity1
Metal bindingi1399Magnesium or manganese 1By similarity1
Metal bindingi1706Magnesium or manganese 2By similarity1
Sitei1807Important for activityBy similarity1
Metal bindingi1811Magnesium or manganese 2By similarity1
Metal bindingi1814Magnesium or manganese 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 71ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Helicase, Hydrolase, Nuclease, RNA-binding
Biological processRNA-mediated gene silencing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease Dicer (EC:3.1.26.3)
Gene namesi
Name:DICER1
Synonyms:DICER
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003739821 – 1923Endoribonuclease DicerAdd BLAST1923

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei413PhosphoserineBy similarity1
Modified residuei415PhosphoserineBy similarity1
Modified residuei1015PhosphoserineBy similarity1
Modified residuei1160PhosphoserineBy similarity1
Modified residuei1461PhosphoserineBy similarity1
Modified residuei1469PhosphoserineBy similarity1
Modified residuei1471PhosphoserineBy similarity1
Modified residuei1869PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6TUI4
PRIDEiQ6TUI4

Interactioni

Subunit structurei

Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Interacts with AGO2, TARBP2, EIF6, MOV10 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC). Interacts with BCDIN3D (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017080

Structurei

3D structure databases

ProteinModelPortaliQ6TUI4
SMRiQ6TUI4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 227Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST177
Domaini433 – 602Helicase C-terminalPROSITE-ProRule annotationAdd BLAST170
Domaini629 – 721Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST93
Domaini890 – 1041PAZPROSITE-ProRule annotationAdd BLAST152
Domaini1276 – 1404RNase III 1PROSITE-ProRule annotationAdd BLAST129
Domaini1667 – 1825RNase III 2PROSITE-ProRule annotationAdd BLAST159
Domaini1853 – 1915DRBMPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni256 – 595Required for interaction with PRKRA and TARBP2By similarityAdd BLAST340

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi175 – 178DECH box4

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0701 Eukaryota
COG0571 LUCA
COG1111 LUCA
HOGENOMiHOG000001567
InParanoidiQ6TUI4
KOiK11592

Family and domain databases

CDDicd00079 HELICc, 1 hit
cd00593 RIBOc, 2 hits
Gene3Di1.10.1520.10, 1 hit
3.30.160.380, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR038248 Dicer_dimer_sf
IPR005034 Dicer_dimerisation_dom
IPR014720 dsRBD_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF03368 Dicer_dimer, 1 hit
PF00271 Helicase_C, 1 hit
PF02170 PAZ, 1 hit
PF00636 Ribonuclease_3, 2 hits
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00358 DSRM, 1 hit
SM00490 HELICc, 1 hit
SM00949 PAZ, 1 hit
SM00535 RIBOc, 2 hits
SUPFAMiSSF101690 SSF101690, 1 hit
SSF52540 SSF52540, 2 hits
SSF69065 SSF69065, 4 hits
PROSITEiView protein in PROSITE
PS51327 DICER_DSRBF, 1 hit
PS50137 DS_RBD, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50821 PAZ, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q6TUI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE
60 70 80 90 100
LLEAALDHNT IVCLNTGSGK TFIAVLLTKE LSYQIRGDFN RNGKRTVFLV
110 120 130 140 150
NSANQVAQQV SAVRTHSDLK VGEYSNLEVS ASWTKEKWNQ EFTKHQVLIM
160 170 180 190 200
TCYVALNVLK NGYLSLSDIN LLVFDECHLA ILDHPYREIM KLCENCPSCP
210 220 230 240 250
RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL VVLDRYTSQP
260 270 280 290 300
CEIVVDCGPF TDRSGLYERL LMELEEALNF INDCNISVHS KERDSTLISK
310 320 330 340 350
QILSDCRAVL VVLGPWCADK VAGMMVRELQ KHIKHEQEEL HRKFLLFTDT
360 370 380 390 400
FLRKIHALCE EHFSPASLDL KFVTPKVIKL LEILRKYKPY ERQQFESVEW
410 420 430 440 450
YNNRNQDNYV SWSDSEDDEE DEEIEEKEKP ETNFPSPFTN ILCGIIFVER
460 470 480 490 500
RYTAVVLNRL IKEAGKQDPE LAYISSNFIT GHGIGKNQPR NKQMEAEFRK
510 520 530 540 550
QEEVLRKFRA HETNLLIATS IVEEGVDIPK CNLVVRFDLP TEYRSYVQSK
560 570 580 590 600
GRARAPISNY VMLADTDKIK SFEEDLKTYK AIEKILRNKC SKSVDTGEAD
610 620 630 640 650
TEPVVDDDDV FPPYVLRPED GPRVTINTAI GHVNRYCARL PSDPFTHLAP
660 670 680 690 700
KCRTRELPDG TFYSTLYLPI NSPLRASIVG PPMSCIRLAE RVVALICCEK
710 720 730 740 750
LHKIGELDDH LMPVGKETVK YEEELDLHDE EETSVPGRPG STKRRQCYPK
760 770 780 790 800
AIPECLRESY PRPGQPCYLY VIGMVLTTPL PDELNFRRRK LYPPEDTTRC
810 820 830 840 850
FGILTAKPIP QIPHFPVYTR SGEVTISIEL KKSGFTLSLQ MLELITRLHQ
860 870 880 890 900
YIFSHILRLE KPALEFKPTD ADSAYCVLPL NVVNDSSTLD IDFKFMEDIE
910 920 930 940 950
KSEARIGIPS TKYSKETPFV FKLEDYQDAV IIPRYRNFDQ PHRFYVADVY
960 970 980 990 1000
TDLTPLSKFP SPEYETFAEY YKTKYNLDLT NLNQPLLDVD HTSSRLNLLT
1010 1020 1030 1040 1050
PRHLNQKGKA LPLSSAEKRK AKWESLQNKQ ILVPELCAIH PIPASLWRKA
1060 1070 1080 1090 1100
VCLPSILYRL HCLLTAEELR AQTASDAGVG VRSLPVDFRY PNLDFGWKKS
1110 1120 1130 1140 1150
IDSKSFISIA NSSSAENENY CKHSTIVVPE NAAHQGANRT SPLENHDQMS
1160 1170 1180 1190 1200
VNCRTLFSES PGKLQIEVST DLTAINGLSY NKSLANGSYD LANRDFCQGN
1210 1220 1230 1240 1250
HLNYYKQEIP VQPTTSYPIQ NLYNYENQPK PSDECTLLSN KYLDGNANTS
1260 1270 1280 1290 1300
TSDGSPVTAA VPGTTETGEA PPDRTASEQS PSPGYSSRTL GPNPGLILQA
1310 1320 1330 1340 1350
LTLSNASDGF NLERLEMLGD SFLKHAITTV SLSALILDAH EGRLSYMRSK
1360 1370 1380 1390 1400
KVSNCNLYRL GKKKGLPSRM VVSIFDPPVN GLPPGYVVNQ DKSNTEKWEK
1410 1420 1430 1440 1450
DEMTKDCMLA NGKLDDDFEE EEEEEEDLMW RAHKEDADDE DDFLEYDQEH
1460 1470 1480 1490 1500
IKFIDNMLMG SGAFVKKISL SPFSATDSAY EWKMPKKSSL GSLPFSSDFE
1510 1520 1530 1540 1550
DFDYSSWDAM CYLDPSKAVE EDDFVVGFWN PSEENCGVDT GKQSISYDLH
1560 1570 1580 1590 1600
TEQCIADKSI ADCVEALLGC YLTSCGERAA QLFLCSLGLK VLPVIKRTDR
1610 1620 1630 1640 1650
EKAMCPTREN FTSQQKNLSG SRAAASGAGY RASVLKDLEY GCLKIPPRCM
1660 1670 1680 1690 1700
FDHPEADRTL RHLISGFENF EKKINYRFKN KAYLLQAFTH ASYHYNTITD
1710 1720 1730 1740 1750
CYQRLEFLGD AILDYLITKH LYEDPRQHSP GVLTDLRSAL VNNTIFASLA
1760 1770 1780 1790 1800
VKYDYHKYFK AVSPELFHVI DDFVQFQLEK NEMQGMDSEL RRSEEDEEKE
1810 1820 1830 1840 1850
EDIEVPKAMG DIFESLAGAI YMDSGMSLET VWQVYYPMMR PLIEKFSANV
1860 1870 1880 1890 1900
PRSPVRELLE MEPEITKFSP AERTYDGKVR VTVEVVGKGK FKGVGRSYRI
1910 1920
AKSAAARRAL RSLKANQPQV PNS
Length:1,923
Mass (Da):218,258
Last modified:September 1, 2009 - v3
Checksum:i3580CB8537484BAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY386968 mRNA Translation: AAR26432.1
RefSeqiNP_976235.1, NM_203359.1
UniGeneiBt.45343
Bt.89150

Genome annotation databases

GeneIDi337871
KEGGibta:337871

Similar proteinsi

Entry informationi

Entry nameiDICER_BOVIN
AccessioniPrimary (citable) accession number: Q6TUI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 1, 2009
Last modified: March 28, 2018
This is version 107 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health