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Protein

Endoribonuclease Dicer

Gene

DICER1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1316 – 13161Magnesium or manganese 1By similarity
Metal bindingi1396 – 13961Magnesium or manganese 1By similarity
Metal bindingi1399 – 13991Magnesium or manganese 1By similarity
Metal bindingi1706 – 17061Magnesium or manganese 2By similarity
Sitei1807 – 18071Important for activityBy similarity
Metal bindingi1811 – 18111Magnesium or manganese 2By similarity
Metal bindingi1814 – 18141Magnesium or manganese 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 718ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease Dicer (EC:3.1.26.3)
Gene namesi
Name:DICER1
Synonyms:DICER
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19231923Endoribonuclease DicerPRO_0000373982Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei413 – 4131PhosphoserineBy similarity
Modified residuei415 – 4151PhosphoserineBy similarity
Modified residuei1015 – 10151PhosphoserineBy similarity
Modified residuei1469 – 14691PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6TUI4.
PRIDEiQ6TUI4.

Interactioni

Subunit structurei

Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017080.

Structurei

3D structure databases

ProteinModelPortaliQ6TUI4.
SMRiQ6TUI4. Positions 1655-1917.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 227177Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini433 – 602170Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini629 – 72193Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
BLAST
Domaini890 – 1041152PAZPROSITE-ProRule annotationAdd
BLAST
Domaini1276 – 1404129RNase III 1PROSITE-ProRule annotationAdd
BLAST
Domaini1667 – 1825159RNase III 2PROSITE-ProRule annotationAdd
BLAST
Domaini1853 – 191563DRBMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni256 – 595340Required for interaction with PRKRA and TARBP2By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi175 – 1784DECH box

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0701. Eukaryota.
COG0571. LUCA.
COG1111. LUCA.
HOGENOMiHOG000001567.
InParanoidiQ6TUI4.
KOiK11592.

Family and domain databases

Gene3Di1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014720. dsRBD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6TUI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE
60 70 80 90 100
LLEAALDHNT IVCLNTGSGK TFIAVLLTKE LSYQIRGDFN RNGKRTVFLV
110 120 130 140 150
NSANQVAQQV SAVRTHSDLK VGEYSNLEVS ASWTKEKWNQ EFTKHQVLIM
160 170 180 190 200
TCYVALNVLK NGYLSLSDIN LLVFDECHLA ILDHPYREIM KLCENCPSCP
210 220 230 240 250
RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL VVLDRYTSQP
260 270 280 290 300
CEIVVDCGPF TDRSGLYERL LMELEEALNF INDCNISVHS KERDSTLISK
310 320 330 340 350
QILSDCRAVL VVLGPWCADK VAGMMVRELQ KHIKHEQEEL HRKFLLFTDT
360 370 380 390 400
FLRKIHALCE EHFSPASLDL KFVTPKVIKL LEILRKYKPY ERQQFESVEW
410 420 430 440 450
YNNRNQDNYV SWSDSEDDEE DEEIEEKEKP ETNFPSPFTN ILCGIIFVER
460 470 480 490 500
RYTAVVLNRL IKEAGKQDPE LAYISSNFIT GHGIGKNQPR NKQMEAEFRK
510 520 530 540 550
QEEVLRKFRA HETNLLIATS IVEEGVDIPK CNLVVRFDLP TEYRSYVQSK
560 570 580 590 600
GRARAPISNY VMLADTDKIK SFEEDLKTYK AIEKILRNKC SKSVDTGEAD
610 620 630 640 650
TEPVVDDDDV FPPYVLRPED GPRVTINTAI GHVNRYCARL PSDPFTHLAP
660 670 680 690 700
KCRTRELPDG TFYSTLYLPI NSPLRASIVG PPMSCIRLAE RVVALICCEK
710 720 730 740 750
LHKIGELDDH LMPVGKETVK YEEELDLHDE EETSVPGRPG STKRRQCYPK
760 770 780 790 800
AIPECLRESY PRPGQPCYLY VIGMVLTTPL PDELNFRRRK LYPPEDTTRC
810 820 830 840 850
FGILTAKPIP QIPHFPVYTR SGEVTISIEL KKSGFTLSLQ MLELITRLHQ
860 870 880 890 900
YIFSHILRLE KPALEFKPTD ADSAYCVLPL NVVNDSSTLD IDFKFMEDIE
910 920 930 940 950
KSEARIGIPS TKYSKETPFV FKLEDYQDAV IIPRYRNFDQ PHRFYVADVY
960 970 980 990 1000
TDLTPLSKFP SPEYETFAEY YKTKYNLDLT NLNQPLLDVD HTSSRLNLLT
1010 1020 1030 1040 1050
PRHLNQKGKA LPLSSAEKRK AKWESLQNKQ ILVPELCAIH PIPASLWRKA
1060 1070 1080 1090 1100
VCLPSILYRL HCLLTAEELR AQTASDAGVG VRSLPVDFRY PNLDFGWKKS
1110 1120 1130 1140 1150
IDSKSFISIA NSSSAENENY CKHSTIVVPE NAAHQGANRT SPLENHDQMS
1160 1170 1180 1190 1200
VNCRTLFSES PGKLQIEVST DLTAINGLSY NKSLANGSYD LANRDFCQGN
1210 1220 1230 1240 1250
HLNYYKQEIP VQPTTSYPIQ NLYNYENQPK PSDECTLLSN KYLDGNANTS
1260 1270 1280 1290 1300
TSDGSPVTAA VPGTTETGEA PPDRTASEQS PSPGYSSRTL GPNPGLILQA
1310 1320 1330 1340 1350
LTLSNASDGF NLERLEMLGD SFLKHAITTV SLSALILDAH EGRLSYMRSK
1360 1370 1380 1390 1400
KVSNCNLYRL GKKKGLPSRM VVSIFDPPVN GLPPGYVVNQ DKSNTEKWEK
1410 1420 1430 1440 1450
DEMTKDCMLA NGKLDDDFEE EEEEEEDLMW RAHKEDADDE DDFLEYDQEH
1460 1470 1480 1490 1500
IKFIDNMLMG SGAFVKKISL SPFSATDSAY EWKMPKKSSL GSLPFSSDFE
1510 1520 1530 1540 1550
DFDYSSWDAM CYLDPSKAVE EDDFVVGFWN PSEENCGVDT GKQSISYDLH
1560 1570 1580 1590 1600
TEQCIADKSI ADCVEALLGC YLTSCGERAA QLFLCSLGLK VLPVIKRTDR
1610 1620 1630 1640 1650
EKAMCPTREN FTSQQKNLSG SRAAASGAGY RASVLKDLEY GCLKIPPRCM
1660 1670 1680 1690 1700
FDHPEADRTL RHLISGFENF EKKINYRFKN KAYLLQAFTH ASYHYNTITD
1710 1720 1730 1740 1750
CYQRLEFLGD AILDYLITKH LYEDPRQHSP GVLTDLRSAL VNNTIFASLA
1760 1770 1780 1790 1800
VKYDYHKYFK AVSPELFHVI DDFVQFQLEK NEMQGMDSEL RRSEEDEEKE
1810 1820 1830 1840 1850
EDIEVPKAMG DIFESLAGAI YMDSGMSLET VWQVYYPMMR PLIEKFSANV
1860 1870 1880 1890 1900
PRSPVRELLE MEPEITKFSP AERTYDGKVR VTVEVVGKGK FKGVGRSYRI
1910 1920
AKSAAARRAL RSLKANQPQV PNS
Length:1,923
Mass (Da):218,258
Last modified:September 1, 2009 - v3
Checksum:i3580CB8537484BAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY386968 mRNA. Translation: AAR26432.1.
RefSeqiNP_976235.1. NM_203359.1.
UniGeneiBt.45343.
Bt.89150.

Genome annotation databases

GeneIDi337871.
KEGGibta:337871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY386968 mRNA. Translation: AAR26432.1.
RefSeqiNP_976235.1. NM_203359.1.
UniGeneiBt.45343.
Bt.89150.

3D structure databases

ProteinModelPortaliQ6TUI4.
SMRiQ6TUI4. Positions 1655-1917.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017080.

Proteomic databases

PaxDbiQ6TUI4.
PRIDEiQ6TUI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi337871.
KEGGibta:337871.

Organism-specific databases

CTDi23405.

Phylogenomic databases

eggNOGiKOG0701. Eukaryota.
COG0571. LUCA.
COG1111. LUCA.
HOGENOMiHOG000001567.
InParanoidiQ6TUI4.
KOiK11592.

Family and domain databases

Gene3Di1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014720. dsRBD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Overexpression of bovine Dicer in mammalian cells."
    Golding M.C., Long C.R., Westhusin M.E.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDICER_BOVIN
AccessioniPrimary (citable) accession number: Q6TUI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 1, 2009
Last modified: June 8, 2016
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.