Q6TU48 (MAOX_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADP-dependent malic enzyme
Short name=NADP-ME
EC=1.1.1.40

Alternative name(s):
SrfA-induced gene A protein

Gene names

Name:	malA
Synonyms:	sigA
ORF Names:	DDB_G0272524

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential.
Tissue specificity	Expressed in the fruiting body. Ref.1
Developmental stage	Expressed at late stage of development (20 to 24 hours). Ref.1 Ref.4
Induction	By cAMP and the MADS-box protein srfA. Ref.1 Ref.4
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from sequence or structural similarity. Source: dictyBase malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	NADP-dependent malic enzyme		PRO_0000328379
Regions
	Nucleotide binding	287 – 303	17	NADP By similarity
Sites
	Active site	92	1	Proton donor By similarity
	Active site	163	1	Proton acceptor By similarity
	Metal binding	234	1	Divalent metal cation By similarity
	Metal binding	235	1	Divalent metal cation By similarity
	Metal binding	258	1	Divalent metal cation By similarity
	Binding site	145	1	NAD By similarity
	Binding site	258	1	NAD By similarity
	Binding site	400	1	NAD By similarity
	Site	258	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6TU48 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EE9938C161E7198E	FASTA	544	60,652
	10 20 30 40 50 60 MQNKPSFILR NPSANKGTGF NNEEREKLGL KGLLPPKVES LQEQSDRALS QFTSFNTNLE 70 80 90 100 110 120 RYIFLNCLRD RNETLFYYLL SNNLELMMPI IYTPTVGEAC QKFGNEFRFA QGMYFASQDK 130 140 150 160 170 180 GNIRAMMDNW PAEGVDIIVV SDGSRILGLG DLGTNGMGIP VGKLQLYVAG AGFCPTRTLP 190 200 210 220 230 240 VIIDSGTNTK KYLEDKYYLG ERHPRIPDSE YYPLVDEFLA AAFNKWPKVI VQFEDISNDH 250 260 270 280 290 300 CFNLLDEYRN KYLCFNDDIQ GTGSVILSGF INAVRSVQKP IKEHRMVFLG AGSAGIGVAD 310 320 330 340 350 360 CIMSLFDEAG VSKEEARKSF WFVDSKGLIT TTRGDELTSQ KKQYAREDYT YQLKSLLEVV 370 380 390 400 410 420 RDVKPTAIIG LSGIGGSFSQ EVIEEMAKHV EKPIVFALSN PTTNAECTAE QAYQWTDGRC 430 440 450 460 470 480 IFASGSPFKP VEYKGKTFVP GQGNNMYIFP GLGLAASVCE AKHVTDAMII TAAKTLASFV 490 500 510 520 530 540 EDSEVLTGKI YPGLQHIREI STRIAVKVIE KAYEEGMAQL PRPDNIEALV KSRQYVPSYD KSKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dictyostelium discoideum developmentally regulated genes whose expression is dependent on MADS box transcription factor SrfA." Escalante R., Moreno N., Sastre L. Eukaryot. Cell 2:1327-1335(2003) [PubMed: 14665466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Strain: AX4.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Identification of genes dependent on the MADS box transcription factor SrfA in Dictyostelium discoideum development." Escalante R., Iranfar N., Sastre L., Loomis W.F. Eukaryot. Cell 3:564-566(2004) [PubMed: 15075287] [Abstract] Cited for: DEVELOPMENTAL STAGE, INDUCTION BY SRFA.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY387644 Genomic DNA. Translation: AAQ95658.1. AAFI02000008 Genomic DNA. Translation: EAL71186.1.
RefSeq	XP_645111.1. XM_640019.1.
3D structure databases
HSSP	HSSP built from PDB template 2AW5 based on UniProtKB P48163.
ProteinModelPortal	Q6TU48.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6TU48.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191268; DDB0191268; DDB_G0272524.
GeneID	8618505.
GenomeReviews	Gene locus malA in contig CM000151_GR.
KEGG	ddi:DDB_G0272524.
Organism-specific databases
dictyBase	DDB_G0272524. malA.
Phylogenomic databases
eggNOG	KOG1257.
GeneTree	EPrGT00050000005364.
HOGENOM	HBG289821.
OMA	FESAITH.
PhylomeDB	Q6TU48.
ProtClustDB	PTZ00317.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00029.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PIRSF	PIRSF000106. ME. 1 hit.
PRINTS	PR00072. MALOXRDTASE.
SMART	SM00919. Malic_M. 1 hit. [Graphical view]
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOX_DICDI

Accession

Primary (citable) accession number: Q6TU48
Secondary accession number(s): Q559J5, Q7KWU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)