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Q6TRW4 (PDS5B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sister chromatid cohesion protein PDS5 homolog B
Alternative name(s):
Androgen-induced proliferation inhibitor
Androgen-induced prostate proliferative shutoff-associated protein AS3
Gene names
Name:Pds5b
Synonyms:Aprin, As3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1447 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Plays a role in androgen-induced proliferative arrest in prostate cells. Ref.4

Subunit structure

Interacts with the cohesin complex. Interacts with RAD21; the interaction is direct. Interacts with WAPAL (via FGF motifs) or CDCA5 (via the FGF motif); the interaction is direct, cohesin-dependent and competitive By similarity.

Subcellular location

Nucleus Ref.4.

Tissue specificity

Highly expressed in intact prostate with levels decreasing after castration. Expressed exclusively in prostate cells inhibited from proliferating by long-term androgen exposure. Ref.3

Sequence similarities

Belongs to the PDS5 family.

Contains 3 A.T hook DNA-binding domains.

Contains 1 HEAT repeat.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmitotic sister chromatid cohesion

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell proliferation

Inferred from direct assay Ref.4. Source: UniProtKB

   Cellular_componentnucleus

Inferred from direct assay Ref.4. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q6TRW4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Gene prediction based on EST data.
Isoform 2 Ref.2 (identifier: Q6TRW4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1354-1390: RAESPETSAVESTQSTPQKGRGRPSKTPSPSQPKKNI → S
Isoform 3 Ref.2 (identifier: Q6TRW4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1207-1208: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14471447Sister chromatid cohesion protein PDS5 homolog B
PRO_0000287426

Regions

Repeat383 – 41937HEAT
DNA binding1247 – 125913A.T hook 1
DNA binding1287 – 129913A.T hook 2
DNA binding1371 – 138313A.T hook 3

Amino acid modifications

Modified residue11361N6-acetyllysine By similarity
Modified residue11621Phosphoserine By similarity
Modified residue11661Phosphoserine By similarity UniProtKB Q9NTI5
Modified residue11761Phosphoserine By similarity
Modified residue11821Phosphoserine By similarity
Modified residue11911Phosphoserine By similarity
Modified residue12571Phosphoserine By similarity UniProtKB Q9NTI5
Modified residue12591Phosphoserine By similarity
Modified residue12831Phosphoserine By similarity
Modified residue13571Phosphoserine By similarity UniProtKB Q9NTI5
Modified residue13661Phosphothreonine By similarity
Modified residue13691Phosphothreonine By similarity UniProtKB Q9NTI5
Modified residue13781Phosphoserine By similarity UniProtKB Q9NTI5
Modified residue13801Phosphothreonine By similarity
Modified residue13821Phosphoserine By similarity UniProtKB Q9NTI5

Natural variations

Alternative sequence1207 – 12082Missing in isoform 3. Ref.2
VSP_052407
Alternative sequence1354 – 139037RAESP…PKKNI → S in isoform 2. Ref.2
VSP_052408

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 15, 2007. Version 2.
Checksum: 9EC8B4A03C320428

FASTA1,447164,459
        10         20         30         40         50         60 
MAHSKTRTND GKITYPPGVK EISDKISKEE MVRRLKMVVK TFMDMDQDSE EEKELYLNLA 

        70         80         90        100        110        120 
LHLASDFFLK HPDKDVRLLV ACCLADIFRI YAPEAPYTSP DKLKDIFMFI TRQLKGLEDT 

       130        140        150        160        170        180 
KSPQFNRYFY LLENIAWVKS YNICFELEDS NEIFTQLYRT LFSVINNGHN QKVHMHMVDL 

       190        200        210        220        230        240 
MSSIICEGDT VSQELLDTVL VNLVPAHKNL NKQAYDLAKA LLKRTAQAIE PYITNFFNQV 

       250        260        270        280        290        300 
LMLGKTSISD LSEHVFDLIL ELYNIDSHLL LSVLPQLEFK LKSNDNEERL QVVKLLAKMF 

       310        320        330        340        350        360 
GAKDSELASQ NKPLWQCYLG RFNDIHVPIR LECVKFASHC LMNHPDLAKD LTEYLKVRSH 

       370        380        390        400        410        420 
DPEEAIRHDV IVSIVTAAKK DILLVNDHLL NFVRERTLDK RWRVRKEAMM GLAQIYKKYA 

       430        440        450        460        470        480 
LQSAAGKDAA KQICWVKDKL LHIYYQNSID DRLLVERIFA QYMVPHNLET TERMKCLYYL 

       490        500        510        520        530        540 
YATLDLNAVK ALNEMWKCQN LLRHQVKDLL DLIKQPKTDA SVKAIFSKVM VITRNLPDPG 

       550        560        570        580        590        600 
KAQDFMKKFT QVLEDDEKIR KQLEALVSPT CSCKQAEGCV REITKKLGNP KQPTNPFLEM 

       610        620        630        640        650        660 
IKFLLERIAP VHIDTESISA LIKQVNKSID GTADDEDEGV PTDQAIRAGL ELLKVLSFTH 

       670        680        690        700        710        720 
PISFHSAETF ESLLACLKMD DEKVAEAALQ IFKNTGSKIE EDFPHIRSAL LPVLHHKSKK 

       730        740        750        760        770        780 
GPPRQAKYAI HCIHAIFSSK ETQFAQIFEP LHKSLDPSNL EHLITPLVTI GHIALLAPDQ 

       790        800        810        820        830        840 
FAAPLKSLVA TFIVKDLLMN DRLPGKKTTK LWVPDEEVSP ETMVKIQAIK MMVRWLLGMK 

       850        860        870        880        890        900 
NNHSKSGTST LRLLTTILHS DGDLTEQGKI SKPDMSRLRL AAGSAIVKLA QEPCYHEIIT 

       910        920        930        940        950        960 
LEQYQLCALA INDECYQVRQ VFAQKLHKGL SRLRLPLEYM AICALCAKDP VKERRAHARQ 

       970        980        990       1000       1010       1020 
CLVKNITVRR EYLKQHAAVS EKLLSLLPEY VVPYTIHLLA HDPDYVKVQD IEQLKDVKEC 

      1030       1040       1050       1060       1070       1080 
LWFVLEILMA KNENNSHAFI RKMVENIKQT KDAQGPDDTK MNEKLYTVCD VAMNIIMSKS 

      1090       1100       1110       1120       1130       1140 
TTYSLESPKD PVLPARFFTQ PDKNFSNTKN YLPPEMKSFF TPGKPKTANV LGAVNKPLSS 

      1150       1160       1170       1180       1190       1200 
AGKQSQTKSS RMETVSNASS SSNPSSPGRI KGRLDSTEMD HSENEDYTMS SPLPGKKSDK 

      1210       1220       1230       1240       1250       1260 
REDSDLVRSE LEKPRSRKKA SVTDPEEKLG MDDLSKLVQE QKPKGSQRGR KRGHAASESE 

      1270       1280       1290       1300       1310       1320 
EQQWPEEKRH KEELLGNEDE QNSPPKKGKR GRPPKPLGGT SKEEPVVKTS KKGNKKKPAP 

      1330       1340       1350       1360       1370       1380 
PVVDEDEEEE RQMGNTEQKS KSKQQRTSKR AQQRAESPET SAVESTQSTP QKGRGRPSKT 

      1390       1400       1410       1420       1430       1440 
PSPSQPKKNI RVGRSKQVAT KENDSSEEMD VLQASSPVSD DTTQEGAEEE DISAGNVRRR 


SSKRERR 

« Hide

Isoform 2 [UniParc].

Checksum: 74871AAFFA93E1ED
Show »

FASTA1,411160,614
Isoform 3 [UniParc].

Checksum: 5C63A886EC3E7C8F
Show »

FASTA1,445164,204

References

« Hide 'large scale' references
[1]"Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. expand/collapse author list , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[2]"Aprin expression in rat olfactory epithelium."
Weiler E., Farbman A.I.
Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-283, NUCLEOTIDE SEQUENCE [MRNA] OF 1110-1378 (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 1110-1264 (ISOFORM 3), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1327-1381 (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 1334-1420 (ISOFORM 2).
Strain: Sprague-Dawley and Wistar.
Tissue: Liver and Testis.
[3]"Expression of novel genes linked to the androgen-induced, proliferative shutoff in prostate cancer cells."
Geck P., Szelei J., Jimenez J., Lin T.-M., Sonnenschein C., Soto A.M.
J. Steroid Biochem. Mol. Biol. 63:211-218(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Mechanism of androgen action on cell proliferation: AS3 protein as a mediator of proliferative arrest in the rat prostate."
Maffini M.V., Geck P., Powell C.E., Sonnenschein C., Soto A.M.
Endocrinology 143:2708-2714(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AABR03082233 Genomic DNA. No translation available.
AABR03082545 Genomic DNA. No translation available.
AY388627 mRNA. Translation: AAQ91374.1.
AY820182 mRNA. Translation: AAV68352.1.
AY820183 mRNA. Translation: AAV68353.1.
AY831451 Genomic DNA. Translation: AAW69306.1.
AY831452 mRNA. Translation: AAW69307.1.
AY836673 mRNA. Translation: AAW69308.1.
UniGeneRn.105768.

3D structure databases

ProteinModelPortalQ6TRW4.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ6TRW4.

Proteomic databases

PaxDbQ6TRW4.
PRIDEQ6TRW4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:1310838. rat. [Q6TRW4-1]

Organism-specific databases

RGD1310838. Pds5b.

Phylogenomic databases

eggNOGNOG268017.
HOGENOMHOG000230672.
HOVERGENHBG108241.

Gene expression databases

GenevestigatorQ6TRW4.

Family and domain databases

Gene3D1.25.10.10. 4 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR017956. AT_hook_DNA-bd_motif.
[Graphical view]
SMARTSM00384. AT_hook. 2 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 5 hits.
ProtoNetSearch...

Other

PROQ6TRW4.

Entry information

Entry namePDS5B_RAT
AccessionPrimary (citable) accession number: Q6TRW4
Secondary accession number(s): Q5G5U1 expand/collapse secondary AC list , Q5G6V7, Q5G6V8, Q5PY35, Q5PY36
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: March 19, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families