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Q6TQR6

- L_CCHFI

UniProt

Q6TQR6 - L_CCHFI

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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Displays RNA-directed RNA polymerase, deubiquitinating and deISGylase activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401For ubiquitin thioesterase activityCurated
Active sitei151 – 1511For ubiquitin thioesterase activityCurated

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  2. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  3. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
  5. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Protein family/group databases

MEROPSiC87.001.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 2 domains:
Ubiquitin thioesterase (EC:3.4.19.12)
Gene namesi
Name:L
OrganismiCrimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV)
Taxonomic identifieri652961 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeNairovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Capra hircus (Goat) [TaxID: 9925]
Homo sapiens (Human) [TaxID: 9606]
Hyalomma [TaxID: 34625]
Ovis aries (Sheep) [TaxID: 9940]
Rhipicephalus microplus (Cattle tick) (Boophilus microplus) [TaxID: 6941]
ProteomesiUP000008767: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi40 – 401C → A: Loss of deubiquitinating and deISGylase activities. 1 Publication
Mutagenesisi151 – 1511H → A: Loss of deubiquitinating and deISGylase activities; when associated with Ala-40. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 39453945RNA-directed RNA polymerase LPRO_0000396080Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-60307N.

Structurei

Secondary structure

1
3945
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 64
Beta strandi10 – 134
Beta strandi16 – 194
Helixi25 – 284
Beta strandi29 – 324
Beta strandi36 – 383
Helixi40 – 4910
Turni55 – 573
Helixi58 – 7215
Helixi73 – 753
Helixi79 – 824
Helixi86 – 938
Helixi102 – 11211
Beta strandi116 – 1205
Beta strandi129 – 1357
Turni138 – 1403
Beta strandi141 – 1477
Turni148 – 1503
Beta strandi151 – 1577
Helixi159 – 1613
Helixi171 – 18212

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PHUX-ray2.20A/B1-217[»]
3PHWX-ray2.00A/C/E/G1-183[»]
3PHXX-ray1.60A1-183[»]
3ZNHX-ray2.30A1-183[»]
ProteinModelPortaliQ6TQR6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6TQR6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 158130OTUPROSITE-ProRule annotationAdd
BLAST
Domaini2342 – 2551210RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi183 – 1864Poly-Ser
Compositional biasi2698 – 274548Ser-richAdd
BLAST

Domaini

The OTU domain is responsible for the deubiquitinating and deISGylation activities of Nsp2.Curated

Sequence similaritiesi

Belongs to the nairovirus RNA polymerase family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR003323. OTU.
IPR015843. RNA-dir_pol_nairovirus.
IPR007099. RNA-dir_pol_NSvirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view]
PfamiPF04196. Bunya_RdRp. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view]
PIRSFiPIRSF036900. RdRPol_NRV. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6TQR6-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDFLRSLDWT QVIAGQYVSN PRFNISDYFE IVRQPGDGNC FYHSIAELTM
60 70 80 90 100
PNKTDHSYHY IKRLTESAAR KYYQEEPEAR LVGLSLEDYL KRMLSDNEWG
110 120 130 140 150
STLEASMLAK EMGITIIIWT VAASDEVEAG IKFGDGDVFT AVNLLHSGQT
160 170 180 190 200
HFDALRILPQ FETDTREALS LMDRVIAVDQ LTSSSSDELQ DYEDLALALT
210 220 230 240 250
SAEESNRRSS LDEVTLSKKQ AEILRQKASQ LSKLVNKSQN IPTRVGRVLD
260 270 280 290 300
CMFNCKLCVE ISADTLILRP ESKEKIGEIM SLRQLGHKLL TRDKQIKQEF
310 320 330 340 350
SRMKLYVTKD LLDHLDVGGL LRAAFPGTGI ERHMQLLHSE MILDICTVSL
360 370 380 390 400
GVMLSTFLYG SNNKNKKKFI TNCLLSTALS GKKVYKVLGN LGNELLYKAP
410 420 430 440 450
RKALATVCSA LFGKQINKLQ NCFRTISPVS LLALRNLDFD CLSVQDYNGM
460 470 480 490 500
IENMSKLDNT DVEFNHREIA DLNQLTSRLI TLRKEKDTDL LKQWFPESDL
510 520 530 540 550
TRRSIRNAAN AEEFVISEFF KKKDIMKFIS TSGRAMSAGK IGNVLSYAHN
560 570 580 590 600
LYLSKSSLNM TSEDISQLLI EIKRLYALQE DSEVEPIAII CDGIESNMKQ
610 620 630 640 650
LFAILPPDCA RECEVLFDDI RNSPTHSTAW KHALRLKGTA YEGLFANCYG
660 670 680 690 700
WQYIPEDIKP SLTMLIQTLF PDKFEDFLDR TQLHPEFRDL TPDFSLTQKV
710 720 730 740 750
HFKRNQIPSV ENVQISIDAT LPESVEAVPV TERKMFPLPE TPLSEVHSIE
760 770 780 790 800
RIMENFTRLM HGGRLSTKKR DGDPAEQGNQ QSITEHESSS ISAFKDYGER
810 820 830 840 850
GIVEENHMKF SGEDQLETRQ LLLVEVGFQT DIDGKIRTDH KKWKDILKLL
860 870 880 890 900
ELLGIKCSFI ACADCSSTPP DRWWITEDRV RVLKNSVSFL FNKLSRNSPT
910 920 930 940 950
EVTDIVVGAI STQKVRSYLK AGTATKTPVS TKDVLETWEK MKEHILNRPT
960 970 980 990 1000
GLTLPTSLEQ AMRKGLVEGV VISKEGSESC INMLKENLDR ITDEFERTKF
1010 1020 1030 1040 1050
KHELTQNITT SEKMLLSWLS EDIKSSRCGE CLSNIKKAVD ETANLSGKIE
1060 1070 1080 1090 1100
LLAYNLQLTN HCSNCHPNGV NISNTSNVCK RCPKIEVVSH CENKGFEDSN
1110 1120 1130 1140 1150
ECLTDLDRLV RLTLPGKTEK ERRVKRNVEY LIKLMMSMSG IDCIKYPTGQ
1160 1170 1180 1190 1200
LITHGRVSAK HNDGNLKDRS DDDQRLAEKI DTVRKELSES KLKDYSTYAR
1210 1220 1230 1240 1250
GVISNSLKNL SRQGKSKCSV PRSWLEKVLF DLKVPTKDEE VLINIRNSLK
1260 1270 1280 1290 1300
ARSEFVRNND KLLIRSKEEL KKCFDVQSFK LKKNKQPVPF QVDCILFKEV
1310 1320 1330 1340 1350
AAECMKRYIG TPYEGIVDTL VSLINVLTRF TWFQEVVLYG KICETFLRCC
1360 1370 1380 1390 1400
TEFNRSGVKL VKIRHCNINL SVKLPSNKKE NMLCCLYSGN MELLQGPFYL
1410 1420 1430 1440 1450
NRRQAVLGSS YLYIVITLYI QVLQQYRCLE VINSVSEKTL QDIENHSMTL
1460 1470 1480 1490 1500
LEDSFREITF ALEGRFEESY KIRTSRCRAS GNFLNRSSRD HFISVVSGLN
1510 1520 1530 1540 1550
LVYGFLIKDN LLANSQQQNK QLQMLRFGML AGLSRLVCPN ELGKKFSTSC
1560 1570 1580 1590 1600
RRIEDNIARL YLQTSIYCSV RDVEDNVKHW KQRDLCPEVT IPCFTVYGTF
1610 1620 1630 1640 1650
VNSDRQLIFD IYNVHIYNKE MDNFDEGCIS VLEETAERHM LWELDLMNSL
1660 1670 1680 1690 1700
CSDEKKDTRP ARLLLGCPNV RKAATREGKK LLKLNSDTST DTQSIASEVS
1710 1720 1730 1740 1750
DRRSYSSSKS RIRSIFGRYN SQKKPFELRS GLEVFNDPFN DYQQAITDIC
1760 1770 1780 1790 1800
QFSEYTPNKE SILKDCLQII RKNPSHTMGS FELIQAISEF GMSKFPPENI
1810 1820 1830 1840 1850
DKARRDPKNW VSISEVTETT SIVASPRTHM MLKDCFKIIL GTENKKIVKM
1860 1870 1880 1890 1900
LRGKLKKLGA ISTNIEIGKR DCLDLLSTVD GLTDQQKENI VNGIFEPSKL
1910 1920 1930 1940 1950
SFYHWKELVK KNIDEVLLTE DGNLIFCWLK TISSSVKGSL KKRLKFMNIH
1960 1970 1980 1990 2000
SPELMPENCL FSSEEFNELI KLKKLLLNEQ QDEQELKQDL LISSWIKCIT
2010 2020 2030 2040 2050
ACKDFASIND KIQKFIYHLS EELYDIRLQH LELSKLKQEH PSVSFTKEEV
2060 2070 2080 2090 2100
LIKRLEKNFL KQHNLEIMET VNLVFFAALS APWCLHYKAL ESYLVRHPEI
2110 2120 2130 2140 2150
LDCGSKEDCK LTLLDLSVSK LLVCLYQKDD EELINSSSLK LGFLVKYVVT
2160 2170 2180 2190 2200
LFTSNGEPFS LSLNDGGLDL DLHKTTDEKL LHQTKIVFAK IGLSGNSYDF
2210 2220 2230 2240 2250
IWTTQMIANS NFNVCKRLTG RSTGERLPRS VRSKVIYEMV KLVGETGMAI
2260 2270 2280 2290 2300
LQQLAFAQAL NYEHRFYAVL APKAQLGGAR DLLVQETGTK VMHATTEMFS
2310 2320 2330 2340 2350
RNLLKTTSDD GLTNPHLKET ILNVGLDCLA NMRNLDGKPI SEGSNLVNFY
2360 2370 2380 2390 2400
KVICISGDNT KWGPIHCCSF FSGMMQQVLK NVPDWCSFYK LTFIKNLCRQ
2410 2420 2430 2440 2450
VEIPAGSIKK ILNVLRYRLC SKGGVEQHSE EDLRRLLTDN LDSWDGNDTV
2460 2470 2480 2490 2500
KFLVTTYISK GLMALNSYNH MGQGIHHATS SVLTSLAAVL FEELAIFYLK
2510 2520 2530 2540 2550
RSLPQTTVHV EHAGSSDDYA KCIVVTGILS KELYSQYDET FWKHACRLKN
2560 2570 2580 2590 2600
FTAAVQRCCQ MKDSAKTLVS DCFLEFYSEF MMGYRVTPAV IKFMFTGLIN
2610 2620 2630 2640 2650
SSVTSPQSLM QACQVSSQQA MYNSVPLVTN TAFTLLRQQI FFNHVEDFIR
2660 2670 2680 2690 2700
RYGILTLGTL SPFGRLFVPT YSGLVSSAVA LEDAEVIARA AQTLQMNSVS
2710 2720 2730 2740 2750
IQSSSLTTLD SLGRSRTSST AEDSSSVSDT TAASHDSGSS SSSFSFELNR
2760 2770 2780 2790 2800
PLSETELQFI KALSSLKSTQ ACEVIQNRIT GLYCNSNEGP LDRHNVIYSS
2810 2820 2830 2840 2850
RMADSCDWLK DGKRRGNLEL ANRIQSVLCI LIAGYYRSFG GEGTEKQVKA
2860 2870 2880 2890 2900
SLNRDDNKII EDPMIQLIPE KLRRELERLG VSRMEVDELM PSISPDDTLA
2910 2920 2930 2940 2950
QLVAKKLISL NVSTEEYSAE VSRLKQTLTA RNVLHGLAGG IKELSLPIYT
2960 2970 2980 2990 3000
IFMKSYFFKD NVFLSLTDRW STKHSTNYRD SCGKQLKGRI ITKYTHWLDT
3010 3020 3030 3040 3050
FLGCSVSINR HTTVKEPSLF NPNIRCVNLI TFEDGLRELS VIQSHLKVFE
3060 3070 3080 3090 3100
NEFTNLNLQF SDPNRQKLRI VESRPAESEL EANRAVIVKT KLFSATEQVR
3110 3120 3130 3140 3150
LSNNPAVVMG YLLDESAISE VKPTKVDFSN LLKDRFKIMQ FFPSVFTLIK
3160 3170 3180 3190 3200
MLTDESSDSE KSGLSPDLQQ VARYSNHLTL LSRMIQQAKP TVTVFYMLKG
3210 3220 3230 3240 3250
NLMNTEPTVA ELVSYGIKEG RFFRLSDTGV DASTYSVKYW KILHCISAIG
3260 3270 3280 3290 3300
CLPLSQADKS SLLMSFLNWR VNMDIRTSDC PLSSHEASIL SEFDGQVIAN
3310 3320 3330 3340 3350
ILASELSSVK RDSEREGLTD LLDYLNSPTE LLKKKPYLGT TCKFNTWGDS
3360 3370 3380 3390 3400
NRSGKFTYSS RSGESIGIFI AGKLHIHLSS ESVALLCETE RQVLSWMSKR
3410 3420 3430 3440 3450
RTEVITKEQH QLFLSLLPQS HECLQKHKDG SALSVIPDSS NPRLLKFVPL
3460 3470 3480 3490 3500
KKGLAVVKIK KQILTVKKQV VFDAESEPRL QWGHGCLSIV YDETDTQTTY
3510 3520 3530 3540 3550
HENLLKVKHL VDCSTDRKKL LPQSVFSDSK VVLSRIKFKT ELLLNSLTLL
3560 3570 3580 3590 3600
HCFLKHAPSD AIMEVESKSS LLHKYLKSGG VRQRNTEVLF REKLNKVVIK
3610 3620 3630 3640 3650
DNLEQGVEEE IEFCNNLTKT VSENPLPLSC WSEVQNYIED IGFNNVLVNI
3660 3670 3680 3690 3700
DRNTVKSELL WKFTLDTNVS TTSTIKDVRT LVSYVSTETI PKFLLAFLLY
3710 3720 3730 3740 3750
EEVLMNLINQ CKAVKELINS TGLSDLELES LLTLCAFYFQ SECSKRDGPR
3760 3770 3780 3790 3800
CSFAALLSLI HEDWQRIGKN ILVRANNELG DVSLKVNIVL VPLKDMSKPK
3810 3820 3830 3840 3850
SERVVMARRS LNHALSLMFL DEMSLPELKS LSVNCKMGNF EGQECFEFTI
3860 3870 3880 3890 3900
LKDNSARLDY NKLIDHCVDM EKKREAVRAV EDLILMLTGR AVKPSAVTQF
3910 3920 3930 3940
VHGDEQCQEQ ISLDDLMAND TVTDFPDREA EALKTGNLGF NWDSD
Length:3,945
Mass (Da):447,851
Last modified:August 16, 2004 - v2
Checksum:i0F95F82432B0DBE1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY389361 Genomic DNA. Translation: AAR25663.2.
RefSeqiYP_325663.1. NC_005301.3.

Genome annotation databases

GeneIDi2943075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY389361 Genomic DNA. Translation: AAR25663.2 .
RefSeqi YP_325663.1. NC_005301.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3PHU X-ray 2.20 A/B 1-217 [» ]
3PHW X-ray 2.00 A/C/E/G 1-183 [» ]
3PHX X-ray 1.60 A 1-183 [» ]
3ZNH X-ray 2.30 A 1-183 [» ]
ProteinModelPortali Q6TQR6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60307N.

Protein family/group databases

MEROPSi C87.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2943075.

Miscellaneous databases

EvolutionaryTracei Q6TQR6.

Family and domain databases

InterProi IPR003323. OTU.
IPR015843. RNA-dir_pol_nairovirus.
IPR007099. RNA-dir_pol_NSvirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view ]
Pfami PF04196. Bunya_RdRp. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view ]
PIRSFi PIRSF036900. RdRPol_NRV. 1 hit.
PROSITEi PS50802. OTU. 1 hit.
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence determination of the Crimean-Congo hemorrhagic fever virus L segment."
    Kinsella E., Martin S.G., Grolla A., Czub M., Feldmann H., Flick R.
    Virology 321:23-28(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: FUNCTION, MUTAGENESIS OF CYS-40 AND HIS-151.

Entry informationi

Entry nameiL_CCHFI
AccessioniPrimary (citable) accession number: Q6TQR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 16, 2004
Last modified: October 1, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3