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Q6TQR6

- L_CCHFI

UniProt

Q6TQR6 - L_CCHFI

Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 2 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Displays RNA-directed RNA polymerase, deubiquitinating and deISGylase activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401For ubiquitin thioesterase activityCurated
    Active sitei151 – 1511For ubiquitin thioesterase activityCurated

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    2. suppression by virus of host ISG15 activity Source: UniProtKB-KW
    3. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    4. transcription, DNA-templated Source: InterPro
    5. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Protein family/group databases

    MEROPSiC87.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase L
    Short name:
    Protein L
    Alternative name(s):
    Large structural protein
    Replicase
    Transcriptase
    Including the following 2 domains:
    Ubiquitin thioesterase (EC:3.4.19.12)
    Gene namesi
    Name:L
    OrganismiCrimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV)
    Taxonomic identifieri652961 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeNairovirus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    Capra hircus (Goat) [TaxID: 9925]
    Homo sapiens (Human) [TaxID: 9606]
    Hyalomma [TaxID: 34625]
    Ovis aries (Sheep) [TaxID: 9940]
    Rhipicephalus microplus (Cattle tick) (Boophilus microplus) [TaxID: 6941]
    ProteomesiUP000008767: Genome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi40 – 401C → A: Loss of deubiquitinating and deISGylase activities. 1 Publication
    Mutagenesisi151 – 1511H → A: Loss of deubiquitinating and deISGylase activities; when associated with Ala-40. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 39453945RNA-directed RNA polymerase LPRO_0000396080Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-60307N.

    Structurei

    Secondary structure

    1
    3945
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 64
    Beta strandi10 – 134
    Beta strandi16 – 194
    Helixi25 – 284
    Beta strandi29 – 324
    Beta strandi36 – 383
    Helixi40 – 4910
    Turni55 – 573
    Helixi58 – 7215
    Helixi73 – 753
    Helixi79 – 824
    Helixi86 – 938
    Helixi102 – 11211
    Beta strandi116 – 1205
    Beta strandi129 – 1357
    Turni138 – 1403
    Beta strandi141 – 1477
    Turni148 – 1503
    Beta strandi151 – 1577
    Helixi159 – 1613
    Helixi171 – 18212

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3PHUX-ray2.20A/B1-217[»]
    3PHWX-ray2.00A/C/E/G1-183[»]
    3PHXX-ray1.60A1-183[»]
    3ZNHX-ray2.30A1-183[»]
    ProteinModelPortaliQ6TQR6.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6TQR6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 158130OTUPROSITE-ProRule annotationAdd
    BLAST
    Domaini2342 – 2551210RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi183 – 1864Poly-Ser
    Compositional biasi2698 – 274548Ser-richAdd
    BLAST

    Domaini

    The OTU domain is responsible for the deubiquitinating and deISGylation activities of Nsp2.Curated

    Sequence similaritiesi

    Belongs to the nairovirus RNA polymerase family.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR015843. RNA-dir_pol_nairovirus.
    IPR007099. RNA-dir_pol_NSvirus.
    IPR007322. RNA_pol_bunyavir.
    [Graphical view]
    PfamiPF04196. Bunya_RdRp. 1 hit.
    PF02338. OTU. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036900. RdRPol_NRV. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6TQR6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFLRSLDWT QVIAGQYVSN PRFNISDYFE IVRQPGDGNC FYHSIAELTM     50
    PNKTDHSYHY IKRLTESAAR KYYQEEPEAR LVGLSLEDYL KRMLSDNEWG 100
    STLEASMLAK EMGITIIIWT VAASDEVEAG IKFGDGDVFT AVNLLHSGQT 150
    HFDALRILPQ FETDTREALS LMDRVIAVDQ LTSSSSDELQ DYEDLALALT 200
    SAEESNRRSS LDEVTLSKKQ AEILRQKASQ LSKLVNKSQN IPTRVGRVLD 250
    CMFNCKLCVE ISADTLILRP ESKEKIGEIM SLRQLGHKLL TRDKQIKQEF 300
    SRMKLYVTKD LLDHLDVGGL LRAAFPGTGI ERHMQLLHSE MILDICTVSL 350
    GVMLSTFLYG SNNKNKKKFI TNCLLSTALS GKKVYKVLGN LGNELLYKAP 400
    RKALATVCSA LFGKQINKLQ NCFRTISPVS LLALRNLDFD CLSVQDYNGM 450
    IENMSKLDNT DVEFNHREIA DLNQLTSRLI TLRKEKDTDL LKQWFPESDL 500
    TRRSIRNAAN AEEFVISEFF KKKDIMKFIS TSGRAMSAGK IGNVLSYAHN 550
    LYLSKSSLNM TSEDISQLLI EIKRLYALQE DSEVEPIAII CDGIESNMKQ 600
    LFAILPPDCA RECEVLFDDI RNSPTHSTAW KHALRLKGTA YEGLFANCYG 650
    WQYIPEDIKP SLTMLIQTLF PDKFEDFLDR TQLHPEFRDL TPDFSLTQKV 700
    HFKRNQIPSV ENVQISIDAT LPESVEAVPV TERKMFPLPE TPLSEVHSIE 750
    RIMENFTRLM HGGRLSTKKR DGDPAEQGNQ QSITEHESSS ISAFKDYGER 800
    GIVEENHMKF SGEDQLETRQ LLLVEVGFQT DIDGKIRTDH KKWKDILKLL 850
    ELLGIKCSFI ACADCSSTPP DRWWITEDRV RVLKNSVSFL FNKLSRNSPT 900
    EVTDIVVGAI STQKVRSYLK AGTATKTPVS TKDVLETWEK MKEHILNRPT 950
    GLTLPTSLEQ AMRKGLVEGV VISKEGSESC INMLKENLDR ITDEFERTKF 1000
    KHELTQNITT SEKMLLSWLS EDIKSSRCGE CLSNIKKAVD ETANLSGKIE 1050
    LLAYNLQLTN HCSNCHPNGV NISNTSNVCK RCPKIEVVSH CENKGFEDSN 1100
    ECLTDLDRLV RLTLPGKTEK ERRVKRNVEY LIKLMMSMSG IDCIKYPTGQ 1150
    LITHGRVSAK HNDGNLKDRS DDDQRLAEKI DTVRKELSES KLKDYSTYAR 1200
    GVISNSLKNL SRQGKSKCSV PRSWLEKVLF DLKVPTKDEE VLINIRNSLK 1250
    ARSEFVRNND KLLIRSKEEL KKCFDVQSFK LKKNKQPVPF QVDCILFKEV 1300
    AAECMKRYIG TPYEGIVDTL VSLINVLTRF TWFQEVVLYG KICETFLRCC 1350
    TEFNRSGVKL VKIRHCNINL SVKLPSNKKE NMLCCLYSGN MELLQGPFYL 1400
    NRRQAVLGSS YLYIVITLYI QVLQQYRCLE VINSVSEKTL QDIENHSMTL 1450
    LEDSFREITF ALEGRFEESY KIRTSRCRAS GNFLNRSSRD HFISVVSGLN 1500
    LVYGFLIKDN LLANSQQQNK QLQMLRFGML AGLSRLVCPN ELGKKFSTSC 1550
    RRIEDNIARL YLQTSIYCSV RDVEDNVKHW KQRDLCPEVT IPCFTVYGTF 1600
    VNSDRQLIFD IYNVHIYNKE MDNFDEGCIS VLEETAERHM LWELDLMNSL 1650
    CSDEKKDTRP ARLLLGCPNV RKAATREGKK LLKLNSDTST DTQSIASEVS 1700
    DRRSYSSSKS RIRSIFGRYN SQKKPFELRS GLEVFNDPFN DYQQAITDIC 1750
    QFSEYTPNKE SILKDCLQII RKNPSHTMGS FELIQAISEF GMSKFPPENI 1800
    DKARRDPKNW VSISEVTETT SIVASPRTHM MLKDCFKIIL GTENKKIVKM 1850
    LRGKLKKLGA ISTNIEIGKR DCLDLLSTVD GLTDQQKENI VNGIFEPSKL 1900
    SFYHWKELVK KNIDEVLLTE DGNLIFCWLK TISSSVKGSL KKRLKFMNIH 1950
    SPELMPENCL FSSEEFNELI KLKKLLLNEQ QDEQELKQDL LISSWIKCIT 2000
    ACKDFASIND KIQKFIYHLS EELYDIRLQH LELSKLKQEH PSVSFTKEEV 2050
    LIKRLEKNFL KQHNLEIMET VNLVFFAALS APWCLHYKAL ESYLVRHPEI 2100
    LDCGSKEDCK LTLLDLSVSK LLVCLYQKDD EELINSSSLK LGFLVKYVVT 2150
    LFTSNGEPFS LSLNDGGLDL DLHKTTDEKL LHQTKIVFAK IGLSGNSYDF 2200
    IWTTQMIANS NFNVCKRLTG RSTGERLPRS VRSKVIYEMV KLVGETGMAI 2250
    LQQLAFAQAL NYEHRFYAVL APKAQLGGAR DLLVQETGTK VMHATTEMFS 2300
    RNLLKTTSDD GLTNPHLKET ILNVGLDCLA NMRNLDGKPI SEGSNLVNFY 2350
    KVICISGDNT KWGPIHCCSF FSGMMQQVLK NVPDWCSFYK LTFIKNLCRQ 2400
    VEIPAGSIKK ILNVLRYRLC SKGGVEQHSE EDLRRLLTDN LDSWDGNDTV 2450
    KFLVTTYISK GLMALNSYNH MGQGIHHATS SVLTSLAAVL FEELAIFYLK 2500
    RSLPQTTVHV EHAGSSDDYA KCIVVTGILS KELYSQYDET FWKHACRLKN 2550
    FTAAVQRCCQ MKDSAKTLVS DCFLEFYSEF MMGYRVTPAV IKFMFTGLIN 2600
    SSVTSPQSLM QACQVSSQQA MYNSVPLVTN TAFTLLRQQI FFNHVEDFIR 2650
    RYGILTLGTL SPFGRLFVPT YSGLVSSAVA LEDAEVIARA AQTLQMNSVS 2700
    IQSSSLTTLD SLGRSRTSST AEDSSSVSDT TAASHDSGSS SSSFSFELNR 2750
    PLSETELQFI KALSSLKSTQ ACEVIQNRIT GLYCNSNEGP LDRHNVIYSS 2800
    RMADSCDWLK DGKRRGNLEL ANRIQSVLCI LIAGYYRSFG GEGTEKQVKA 2850
    SLNRDDNKII EDPMIQLIPE KLRRELERLG VSRMEVDELM PSISPDDTLA 2900
    QLVAKKLISL NVSTEEYSAE VSRLKQTLTA RNVLHGLAGG IKELSLPIYT 2950
    IFMKSYFFKD NVFLSLTDRW STKHSTNYRD SCGKQLKGRI ITKYTHWLDT 3000
    FLGCSVSINR HTTVKEPSLF NPNIRCVNLI TFEDGLRELS VIQSHLKVFE 3050
    NEFTNLNLQF SDPNRQKLRI VESRPAESEL EANRAVIVKT KLFSATEQVR 3100
    LSNNPAVVMG YLLDESAISE VKPTKVDFSN LLKDRFKIMQ FFPSVFTLIK 3150
    MLTDESSDSE KSGLSPDLQQ VARYSNHLTL LSRMIQQAKP TVTVFYMLKG 3200
    NLMNTEPTVA ELVSYGIKEG RFFRLSDTGV DASTYSVKYW KILHCISAIG 3250
    CLPLSQADKS SLLMSFLNWR VNMDIRTSDC PLSSHEASIL SEFDGQVIAN 3300
    ILASELSSVK RDSEREGLTD LLDYLNSPTE LLKKKPYLGT TCKFNTWGDS 3350
    NRSGKFTYSS RSGESIGIFI AGKLHIHLSS ESVALLCETE RQVLSWMSKR 3400
    RTEVITKEQH QLFLSLLPQS HECLQKHKDG SALSVIPDSS NPRLLKFVPL 3450
    KKGLAVVKIK KQILTVKKQV VFDAESEPRL QWGHGCLSIV YDETDTQTTY 3500
    HENLLKVKHL VDCSTDRKKL LPQSVFSDSK VVLSRIKFKT ELLLNSLTLL 3550
    HCFLKHAPSD AIMEVESKSS LLHKYLKSGG VRQRNTEVLF REKLNKVVIK 3600
    DNLEQGVEEE IEFCNNLTKT VSENPLPLSC WSEVQNYIED IGFNNVLVNI 3650
    DRNTVKSELL WKFTLDTNVS TTSTIKDVRT LVSYVSTETI PKFLLAFLLY 3700
    EEVLMNLINQ CKAVKELINS TGLSDLELES LLTLCAFYFQ SECSKRDGPR 3750
    CSFAALLSLI HEDWQRIGKN ILVRANNELG DVSLKVNIVL VPLKDMSKPK 3800
    SERVVMARRS LNHALSLMFL DEMSLPELKS LSVNCKMGNF EGQECFEFTI 3850
    LKDNSARLDY NKLIDHCVDM EKKREAVRAV EDLILMLTGR AVKPSAVTQF 3900
    VHGDEQCQEQ ISLDDLMAND TVTDFPDREA EALKTGNLGF NWDSD 3945
    Length:3,945
    Mass (Da):447,851
    Last modified:August 16, 2004 - v2
    Checksum:i0F95F82432B0DBE1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY389361 Genomic DNA. Translation: AAR25663.2.
    RefSeqiYP_325663.1. NC_005301.3.

    Genome annotation databases

    GeneIDi2943075.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY389361 Genomic DNA. Translation: AAR25663.2 .
    RefSeqi YP_325663.1. NC_005301.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3PHU X-ray 2.20 A/B 1-217 [» ]
    3PHW X-ray 2.00 A/C/E/G 1-183 [» ]
    3PHX X-ray 1.60 A 1-183 [» ]
    3ZNH X-ray 2.30 A 1-183 [» ]
    ProteinModelPortali Q6TQR6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60307N.

    Protein family/group databases

    MEROPSi C87.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2943075.

    Miscellaneous databases

    EvolutionaryTracei Q6TQR6.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR015843. RNA-dir_pol_nairovirus.
    IPR007099. RNA-dir_pol_NSvirus.
    IPR007322. RNA_pol_bunyavir.
    [Graphical view ]
    Pfami PF04196. Bunya_RdRp. 1 hit.
    PF02338. OTU. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036900. RdRPol_NRV. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence determination of the Crimean-Congo hemorrhagic fever virus L segment."
      Kinsella E., Martin S.G., Grolla A., Czub M., Feldmann H., Flick R.
      Virology 321:23-28(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Cited for: FUNCTION, MUTAGENESIS OF CYS-40 AND HIS-151.

    Entry informationi

    Entry nameiL_CCHFI
    AccessioniPrimary (citable) accession number: Q6TQR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 54 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3