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Q6TQR6

- L_CCHFI

UniProt

Q6TQR6 - L_CCHFI

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Protein

RNA-directed RNA polymerase L

Gene
L
Organism
Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Displays RNA-directed RNA polymerase, deubiquitinating and deISGylase activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401For ubiquitin thioesterase activity Inferred
Active sitei151 – 1511For ubiquitin thioesterase activity Inferred

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  2. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  3. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
  5. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Protein family/group databases

MEROPSiC87.001.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 2 domains:
Ubiquitin thioesterase (EC:3.4.19.12)
Gene namesi
Name:L
OrganismiCrimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV)
Taxonomic identifieri652961 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeNairovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Capra hircus (Goat) [TaxID: 9925]
Homo sapiens (Human) [TaxID: 9606]
Hyalomma [TaxID: 34625]
Ovis aries (Sheep) [TaxID: 9940]
Rhipicephalus microplus (Cattle tick) (Boophilus microplus) [TaxID: 6941]
ProteomesiUP000008767: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi40 – 401C → A: Loss of deubiquitinating and deISGylase activities. 1 Publication
Mutagenesisi151 – 1511H → A: Loss of deubiquitinating and deISGylase activities; when associated with Ala-40. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 39453945RNA-directed RNA polymerase LPRO_0000396080Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-60307N.

Structurei

Secondary structure

1
3945
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 64
Beta strandi10 – 134
Beta strandi16 – 194
Helixi25 – 284
Beta strandi29 – 324
Beta strandi36 – 383
Helixi40 – 4910
Turni55 – 573
Helixi58 – 7215
Helixi73 – 753
Helixi79 – 824
Helixi86 – 938
Helixi102 – 11211
Beta strandi116 – 1205
Beta strandi129 – 1357
Turni138 – 1403
Beta strandi141 – 1477
Turni148 – 1503
Beta strandi151 – 1577
Helixi159 – 1613
Helixi171 – 18212

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PHUX-ray2.20A/B1-217[»]
3PHWX-ray2.00A/C/E/G1-183[»]
3PHXX-ray1.60A1-183[»]
3ZNHX-ray2.30A1-183[»]
ProteinModelPortaliQ6TQR6.

Miscellaneous databases

EvolutionaryTraceiQ6TQR6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 158130OTUAdd
BLAST
Domaini2342 – 2551210RdRp catalyticAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi183 – 1864Poly-Ser
Compositional biasi2698 – 274548Ser-richAdd
BLAST

Domaini

The OTU domain is responsible for the deubiquitinating and deISGylation activities of Nsp2 Inferred.

Sequence similaritiesi

Contains 1 OTU domain.

Family and domain databases

InterProiIPR003323. OTU.
IPR015843. RNA-dir_pol_nairovirus.
IPR007099. RNA-dir_pol_NSvirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view]
PfamiPF04196. Bunya_RdRp. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view]
PIRSFiPIRSF036900. RdRPol_NRV. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6TQR6-1 [UniParc]FASTAAdd to Basket

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MDFLRSLDWT QVIAGQYVSN PRFNISDYFE IVRQPGDGNC FYHSIAELTM     50
PNKTDHSYHY IKRLTESAAR KYYQEEPEAR LVGLSLEDYL KRMLSDNEWG 100
STLEASMLAK EMGITIIIWT VAASDEVEAG IKFGDGDVFT AVNLLHSGQT 150
HFDALRILPQ FETDTREALS LMDRVIAVDQ LTSSSSDELQ DYEDLALALT 200
SAEESNRRSS LDEVTLSKKQ AEILRQKASQ LSKLVNKSQN IPTRVGRVLD 250
CMFNCKLCVE ISADTLILRP ESKEKIGEIM SLRQLGHKLL TRDKQIKQEF 300
SRMKLYVTKD LLDHLDVGGL LRAAFPGTGI ERHMQLLHSE MILDICTVSL 350
GVMLSTFLYG SNNKNKKKFI TNCLLSTALS GKKVYKVLGN LGNELLYKAP 400
RKALATVCSA LFGKQINKLQ NCFRTISPVS LLALRNLDFD CLSVQDYNGM 450
IENMSKLDNT DVEFNHREIA DLNQLTSRLI TLRKEKDTDL LKQWFPESDL 500
TRRSIRNAAN AEEFVISEFF KKKDIMKFIS TSGRAMSAGK IGNVLSYAHN 550
LYLSKSSLNM TSEDISQLLI EIKRLYALQE DSEVEPIAII CDGIESNMKQ 600
LFAILPPDCA RECEVLFDDI RNSPTHSTAW KHALRLKGTA YEGLFANCYG 650
WQYIPEDIKP SLTMLIQTLF PDKFEDFLDR TQLHPEFRDL TPDFSLTQKV 700
HFKRNQIPSV ENVQISIDAT LPESVEAVPV TERKMFPLPE TPLSEVHSIE 750
RIMENFTRLM HGGRLSTKKR DGDPAEQGNQ QSITEHESSS ISAFKDYGER 800
GIVEENHMKF SGEDQLETRQ LLLVEVGFQT DIDGKIRTDH KKWKDILKLL 850
ELLGIKCSFI ACADCSSTPP DRWWITEDRV RVLKNSVSFL FNKLSRNSPT 900
EVTDIVVGAI STQKVRSYLK AGTATKTPVS TKDVLETWEK MKEHILNRPT 950
GLTLPTSLEQ AMRKGLVEGV VISKEGSESC INMLKENLDR ITDEFERTKF 1000
KHELTQNITT SEKMLLSWLS EDIKSSRCGE CLSNIKKAVD ETANLSGKIE 1050
LLAYNLQLTN HCSNCHPNGV NISNTSNVCK RCPKIEVVSH CENKGFEDSN 1100
ECLTDLDRLV RLTLPGKTEK ERRVKRNVEY LIKLMMSMSG IDCIKYPTGQ 1150
LITHGRVSAK HNDGNLKDRS DDDQRLAEKI DTVRKELSES KLKDYSTYAR 1200
GVISNSLKNL SRQGKSKCSV PRSWLEKVLF DLKVPTKDEE VLINIRNSLK 1250
ARSEFVRNND KLLIRSKEEL KKCFDVQSFK LKKNKQPVPF QVDCILFKEV 1300
AAECMKRYIG TPYEGIVDTL VSLINVLTRF TWFQEVVLYG KICETFLRCC 1350
TEFNRSGVKL VKIRHCNINL SVKLPSNKKE NMLCCLYSGN MELLQGPFYL 1400
NRRQAVLGSS YLYIVITLYI QVLQQYRCLE VINSVSEKTL QDIENHSMTL 1450
LEDSFREITF ALEGRFEESY KIRTSRCRAS GNFLNRSSRD HFISVVSGLN 1500
LVYGFLIKDN LLANSQQQNK QLQMLRFGML AGLSRLVCPN ELGKKFSTSC 1550
RRIEDNIARL YLQTSIYCSV RDVEDNVKHW KQRDLCPEVT IPCFTVYGTF 1600
VNSDRQLIFD IYNVHIYNKE MDNFDEGCIS VLEETAERHM LWELDLMNSL 1650
CSDEKKDTRP ARLLLGCPNV RKAATREGKK LLKLNSDTST DTQSIASEVS 1700
DRRSYSSSKS RIRSIFGRYN SQKKPFELRS GLEVFNDPFN DYQQAITDIC 1750
QFSEYTPNKE SILKDCLQII RKNPSHTMGS FELIQAISEF GMSKFPPENI 1800
DKARRDPKNW VSISEVTETT SIVASPRTHM MLKDCFKIIL GTENKKIVKM 1850
LRGKLKKLGA ISTNIEIGKR DCLDLLSTVD GLTDQQKENI VNGIFEPSKL 1900
SFYHWKELVK KNIDEVLLTE DGNLIFCWLK TISSSVKGSL KKRLKFMNIH 1950
SPELMPENCL FSSEEFNELI KLKKLLLNEQ QDEQELKQDL LISSWIKCIT 2000
ACKDFASIND KIQKFIYHLS EELYDIRLQH LELSKLKQEH PSVSFTKEEV 2050
LIKRLEKNFL KQHNLEIMET VNLVFFAALS APWCLHYKAL ESYLVRHPEI 2100
LDCGSKEDCK LTLLDLSVSK LLVCLYQKDD EELINSSSLK LGFLVKYVVT 2150
LFTSNGEPFS LSLNDGGLDL DLHKTTDEKL LHQTKIVFAK IGLSGNSYDF 2200
IWTTQMIANS NFNVCKRLTG RSTGERLPRS VRSKVIYEMV KLVGETGMAI 2250
LQQLAFAQAL NYEHRFYAVL APKAQLGGAR DLLVQETGTK VMHATTEMFS 2300
RNLLKTTSDD GLTNPHLKET ILNVGLDCLA NMRNLDGKPI SEGSNLVNFY 2350
KVICISGDNT KWGPIHCCSF FSGMMQQVLK NVPDWCSFYK LTFIKNLCRQ 2400
VEIPAGSIKK ILNVLRYRLC SKGGVEQHSE EDLRRLLTDN LDSWDGNDTV 2450
KFLVTTYISK GLMALNSYNH MGQGIHHATS SVLTSLAAVL FEELAIFYLK 2500
RSLPQTTVHV EHAGSSDDYA KCIVVTGILS KELYSQYDET FWKHACRLKN 2550
FTAAVQRCCQ MKDSAKTLVS DCFLEFYSEF MMGYRVTPAV IKFMFTGLIN 2600
SSVTSPQSLM QACQVSSQQA MYNSVPLVTN TAFTLLRQQI FFNHVEDFIR 2650
RYGILTLGTL SPFGRLFVPT YSGLVSSAVA LEDAEVIARA AQTLQMNSVS 2700
IQSSSLTTLD SLGRSRTSST AEDSSSVSDT TAASHDSGSS SSSFSFELNR 2750
PLSETELQFI KALSSLKSTQ ACEVIQNRIT GLYCNSNEGP LDRHNVIYSS 2800
RMADSCDWLK DGKRRGNLEL ANRIQSVLCI LIAGYYRSFG GEGTEKQVKA 2850
SLNRDDNKII EDPMIQLIPE KLRRELERLG VSRMEVDELM PSISPDDTLA 2900
QLVAKKLISL NVSTEEYSAE VSRLKQTLTA RNVLHGLAGG IKELSLPIYT 2950
IFMKSYFFKD NVFLSLTDRW STKHSTNYRD SCGKQLKGRI ITKYTHWLDT 3000
FLGCSVSINR HTTVKEPSLF NPNIRCVNLI TFEDGLRELS VIQSHLKVFE 3050
NEFTNLNLQF SDPNRQKLRI VESRPAESEL EANRAVIVKT KLFSATEQVR 3100
LSNNPAVVMG YLLDESAISE VKPTKVDFSN LLKDRFKIMQ FFPSVFTLIK 3150
MLTDESSDSE KSGLSPDLQQ VARYSNHLTL LSRMIQQAKP TVTVFYMLKG 3200
NLMNTEPTVA ELVSYGIKEG RFFRLSDTGV DASTYSVKYW KILHCISAIG 3250
CLPLSQADKS SLLMSFLNWR VNMDIRTSDC PLSSHEASIL SEFDGQVIAN 3300
ILASELSSVK RDSEREGLTD LLDYLNSPTE LLKKKPYLGT TCKFNTWGDS 3350
NRSGKFTYSS RSGESIGIFI AGKLHIHLSS ESVALLCETE RQVLSWMSKR 3400
RTEVITKEQH QLFLSLLPQS HECLQKHKDG SALSVIPDSS NPRLLKFVPL 3450
KKGLAVVKIK KQILTVKKQV VFDAESEPRL QWGHGCLSIV YDETDTQTTY 3500
HENLLKVKHL VDCSTDRKKL LPQSVFSDSK VVLSRIKFKT ELLLNSLTLL 3550
HCFLKHAPSD AIMEVESKSS LLHKYLKSGG VRQRNTEVLF REKLNKVVIK 3600
DNLEQGVEEE IEFCNNLTKT VSENPLPLSC WSEVQNYIED IGFNNVLVNI 3650
DRNTVKSELL WKFTLDTNVS TTSTIKDVRT LVSYVSTETI PKFLLAFLLY 3700
EEVLMNLINQ CKAVKELINS TGLSDLELES LLTLCAFYFQ SECSKRDGPR 3750
CSFAALLSLI HEDWQRIGKN ILVRANNELG DVSLKVNIVL VPLKDMSKPK 3800
SERVVMARRS LNHALSLMFL DEMSLPELKS LSVNCKMGNF EGQECFEFTI 3850
LKDNSARLDY NKLIDHCVDM EKKREAVRAV EDLILMLTGR AVKPSAVTQF 3900
VHGDEQCQEQ ISLDDLMAND TVTDFPDREA EALKTGNLGF NWDSD 3945
Length:3,945
Mass (Da):447,851
Last modified:August 16, 2004 - v2
Checksum:i0F95F82432B0DBE1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY389361 Genomic DNA. Translation: AAR25663.2.
RefSeqiYP_325663.1. NC_005301.3.

Genome annotation databases

GeneIDi2943075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY389361 Genomic DNA. Translation: AAR25663.2 .
RefSeqi YP_325663.1. NC_005301.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3PHU X-ray 2.20 A/B 1-217 [» ]
3PHW X-ray 2.00 A/C/E/G 1-183 [» ]
3PHX X-ray 1.60 A 1-183 [» ]
3ZNH X-ray 2.30 A 1-183 [» ]
ProteinModelPortali Q6TQR6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60307N.

Protein family/group databases

MEROPSi C87.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2943075.

Miscellaneous databases

EvolutionaryTracei Q6TQR6.

Family and domain databases

InterProi IPR003323. OTU.
IPR015843. RNA-dir_pol_nairovirus.
IPR007099. RNA-dir_pol_NSvirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view ]
Pfami PF04196. Bunya_RdRp. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view ]
PIRSFi PIRSF036900. RdRPol_NRV. 1 hit.
PROSITEi PS50802. OTU. 1 hit.
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence determination of the Crimean-Congo hemorrhagic fever virus L segment."
    Kinsella E., Martin S.G., Grolla A., Czub M., Feldmann H., Flick R.
    Virology 321:23-28(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: FUNCTION, MUTAGENESIS OF CYS-40 AND HIS-151.

Entry informationi

Entry nameiL_CCHFI
AccessioniPrimary (citable) accession number: Q6TQR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi