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Q6TQR6 (L_CCHFI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase L

Short name=Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 2 domains:

  1. Ubiquitin thioesterase
    EC=3.4.19.12
  2. RNA-directed RNA polymerase
    EC=2.7.7.48
Gene names
Name:L
OrganismCrimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV) [Reference proteome]
Taxonomic identifier652961 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesBunyaviridaeNairovirus
Virus hostBos taurus (Bovine) [TaxID: 9913]
Capra hircus (Goat) [TaxID: 9925]
Homo sapiens (Human) [TaxID: 9606]
Hyalomma [TaxID: 34625]
Ovis aries (Sheep) [TaxID: 9940]
Rhipicephalus microplus (Cattle tick) (Boophilus microplus) [TaxID: 6941]

Protein attributes

Sequence length3945 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Displays RNA-directed RNA polymerase, deubiquitinating and deISGylase activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity. Ref.2

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Domain

The OTU domain is responsible for the deubiquitinating and deISGylation activities of Nsp2 Probable.

Sequence similarities

Belongs to the nairovirus RNA polymerase family.

Contains 1 OTU domain.

Contains 1 RdRp catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 39453945RNA-directed RNA polymerase L
PRO_0000396080

Regions

Domain29 – 158130OTU
Domain2342 – 2551210RdRp catalytic
Compositional bias183 – 1864Poly-Ser
Compositional bias2698 – 274548Ser-rich

Sites

Active site401For ubiquitin thioesterase activity Probable
Active site1511For ubiquitin thioesterase activity Probable

Experimental info

Mutagenesis401C → A: Loss of deubiquitinating and deISGylase activities. Ref.2
Mutagenesis1511H → A: Loss of deubiquitinating and deISGylase activities; when associated with Ala-40. Ref.2

Secondary structure

..................................... 3945
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q6TQR6 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: 0F95F82432B0DBE1

FASTA3,945447,851
        10         20         30         40         50         60 
MDFLRSLDWT QVIAGQYVSN PRFNISDYFE IVRQPGDGNC FYHSIAELTM PNKTDHSYHY 

        70         80         90        100        110        120 
IKRLTESAAR KYYQEEPEAR LVGLSLEDYL KRMLSDNEWG STLEASMLAK EMGITIIIWT 

       130        140        150        160        170        180 
VAASDEVEAG IKFGDGDVFT AVNLLHSGQT HFDALRILPQ FETDTREALS LMDRVIAVDQ 

       190        200        210        220        230        240 
LTSSSSDELQ DYEDLALALT SAEESNRRSS LDEVTLSKKQ AEILRQKASQ LSKLVNKSQN 

       250        260        270        280        290        300 
IPTRVGRVLD CMFNCKLCVE ISADTLILRP ESKEKIGEIM SLRQLGHKLL TRDKQIKQEF 

       310        320        330        340        350        360 
SRMKLYVTKD LLDHLDVGGL LRAAFPGTGI ERHMQLLHSE MILDICTVSL GVMLSTFLYG 

       370        380        390        400        410        420 
SNNKNKKKFI TNCLLSTALS GKKVYKVLGN LGNELLYKAP RKALATVCSA LFGKQINKLQ 

       430        440        450        460        470        480 
NCFRTISPVS LLALRNLDFD CLSVQDYNGM IENMSKLDNT DVEFNHREIA DLNQLTSRLI 

       490        500        510        520        530        540 
TLRKEKDTDL LKQWFPESDL TRRSIRNAAN AEEFVISEFF KKKDIMKFIS TSGRAMSAGK 

       550        560        570        580        590        600 
IGNVLSYAHN LYLSKSSLNM TSEDISQLLI EIKRLYALQE DSEVEPIAII CDGIESNMKQ 

       610        620        630        640        650        660 
LFAILPPDCA RECEVLFDDI RNSPTHSTAW KHALRLKGTA YEGLFANCYG WQYIPEDIKP 

       670        680        690        700        710        720 
SLTMLIQTLF PDKFEDFLDR TQLHPEFRDL TPDFSLTQKV HFKRNQIPSV ENVQISIDAT 

       730        740        750        760        770        780 
LPESVEAVPV TERKMFPLPE TPLSEVHSIE RIMENFTRLM HGGRLSTKKR DGDPAEQGNQ 

       790        800        810        820        830        840 
QSITEHESSS ISAFKDYGER GIVEENHMKF SGEDQLETRQ LLLVEVGFQT DIDGKIRTDH 

       850        860        870        880        890        900 
KKWKDILKLL ELLGIKCSFI ACADCSSTPP DRWWITEDRV RVLKNSVSFL FNKLSRNSPT 

       910        920        930        940        950        960 
EVTDIVVGAI STQKVRSYLK AGTATKTPVS TKDVLETWEK MKEHILNRPT GLTLPTSLEQ 

       970        980        990       1000       1010       1020 
AMRKGLVEGV VISKEGSESC INMLKENLDR ITDEFERTKF KHELTQNITT SEKMLLSWLS 

      1030       1040       1050       1060       1070       1080 
EDIKSSRCGE CLSNIKKAVD ETANLSGKIE LLAYNLQLTN HCSNCHPNGV NISNTSNVCK 

      1090       1100       1110       1120       1130       1140 
RCPKIEVVSH CENKGFEDSN ECLTDLDRLV RLTLPGKTEK ERRVKRNVEY LIKLMMSMSG 

      1150       1160       1170       1180       1190       1200 
IDCIKYPTGQ LITHGRVSAK HNDGNLKDRS DDDQRLAEKI DTVRKELSES KLKDYSTYAR 

      1210       1220       1230       1240       1250       1260 
GVISNSLKNL SRQGKSKCSV PRSWLEKVLF DLKVPTKDEE VLINIRNSLK ARSEFVRNND 

      1270       1280       1290       1300       1310       1320 
KLLIRSKEEL KKCFDVQSFK LKKNKQPVPF QVDCILFKEV AAECMKRYIG TPYEGIVDTL 

      1330       1340       1350       1360       1370       1380 
VSLINVLTRF TWFQEVVLYG KICETFLRCC TEFNRSGVKL VKIRHCNINL SVKLPSNKKE 

      1390       1400       1410       1420       1430       1440 
NMLCCLYSGN MELLQGPFYL NRRQAVLGSS YLYIVITLYI QVLQQYRCLE VINSVSEKTL 

      1450       1460       1470       1480       1490       1500 
QDIENHSMTL LEDSFREITF ALEGRFEESY KIRTSRCRAS GNFLNRSSRD HFISVVSGLN 

      1510       1520       1530       1540       1550       1560 
LVYGFLIKDN LLANSQQQNK QLQMLRFGML AGLSRLVCPN ELGKKFSTSC RRIEDNIARL 

      1570       1580       1590       1600       1610       1620 
YLQTSIYCSV RDVEDNVKHW KQRDLCPEVT IPCFTVYGTF VNSDRQLIFD IYNVHIYNKE 

      1630       1640       1650       1660       1670       1680 
MDNFDEGCIS VLEETAERHM LWELDLMNSL CSDEKKDTRP ARLLLGCPNV RKAATREGKK 

      1690       1700       1710       1720       1730       1740 
LLKLNSDTST DTQSIASEVS DRRSYSSSKS RIRSIFGRYN SQKKPFELRS GLEVFNDPFN 

      1750       1760       1770       1780       1790       1800 
DYQQAITDIC QFSEYTPNKE SILKDCLQII RKNPSHTMGS FELIQAISEF GMSKFPPENI 

      1810       1820       1830       1840       1850       1860 
DKARRDPKNW VSISEVTETT SIVASPRTHM MLKDCFKIIL GTENKKIVKM LRGKLKKLGA 

      1870       1880       1890       1900       1910       1920 
ISTNIEIGKR DCLDLLSTVD GLTDQQKENI VNGIFEPSKL SFYHWKELVK KNIDEVLLTE 

      1930       1940       1950       1960       1970       1980 
DGNLIFCWLK TISSSVKGSL KKRLKFMNIH SPELMPENCL FSSEEFNELI KLKKLLLNEQ 

      1990       2000       2010       2020       2030       2040 
QDEQELKQDL LISSWIKCIT ACKDFASIND KIQKFIYHLS EELYDIRLQH LELSKLKQEH 

      2050       2060       2070       2080       2090       2100 
PSVSFTKEEV LIKRLEKNFL KQHNLEIMET VNLVFFAALS APWCLHYKAL ESYLVRHPEI 

      2110       2120       2130       2140       2150       2160 
LDCGSKEDCK LTLLDLSVSK LLVCLYQKDD EELINSSSLK LGFLVKYVVT LFTSNGEPFS 

      2170       2180       2190       2200       2210       2220 
LSLNDGGLDL DLHKTTDEKL LHQTKIVFAK IGLSGNSYDF IWTTQMIANS NFNVCKRLTG 

      2230       2240       2250       2260       2270       2280 
RSTGERLPRS VRSKVIYEMV KLVGETGMAI LQQLAFAQAL NYEHRFYAVL APKAQLGGAR 

      2290       2300       2310       2320       2330       2340 
DLLVQETGTK VMHATTEMFS RNLLKTTSDD GLTNPHLKET ILNVGLDCLA NMRNLDGKPI 

      2350       2360       2370       2380       2390       2400 
SEGSNLVNFY KVICISGDNT KWGPIHCCSF FSGMMQQVLK NVPDWCSFYK LTFIKNLCRQ 

      2410       2420       2430       2440       2450       2460 
VEIPAGSIKK ILNVLRYRLC SKGGVEQHSE EDLRRLLTDN LDSWDGNDTV KFLVTTYISK 

      2470       2480       2490       2500       2510       2520 
GLMALNSYNH MGQGIHHATS SVLTSLAAVL FEELAIFYLK RSLPQTTVHV EHAGSSDDYA 

      2530       2540       2550       2560       2570       2580 
KCIVVTGILS KELYSQYDET FWKHACRLKN FTAAVQRCCQ MKDSAKTLVS DCFLEFYSEF 

      2590       2600       2610       2620       2630       2640 
MMGYRVTPAV IKFMFTGLIN SSVTSPQSLM QACQVSSQQA MYNSVPLVTN TAFTLLRQQI 

      2650       2660       2670       2680       2690       2700 
FFNHVEDFIR RYGILTLGTL SPFGRLFVPT YSGLVSSAVA LEDAEVIARA AQTLQMNSVS 

      2710       2720       2730       2740       2750       2760 
IQSSSLTTLD SLGRSRTSST AEDSSSVSDT TAASHDSGSS SSSFSFELNR PLSETELQFI 

      2770       2780       2790       2800       2810       2820 
KALSSLKSTQ ACEVIQNRIT GLYCNSNEGP LDRHNVIYSS RMADSCDWLK DGKRRGNLEL 

      2830       2840       2850       2860       2870       2880 
ANRIQSVLCI LIAGYYRSFG GEGTEKQVKA SLNRDDNKII EDPMIQLIPE KLRRELERLG 

      2890       2900       2910       2920       2930       2940 
VSRMEVDELM PSISPDDTLA QLVAKKLISL NVSTEEYSAE VSRLKQTLTA RNVLHGLAGG 

      2950       2960       2970       2980       2990       3000 
IKELSLPIYT IFMKSYFFKD NVFLSLTDRW STKHSTNYRD SCGKQLKGRI ITKYTHWLDT 

      3010       3020       3030       3040       3050       3060 
FLGCSVSINR HTTVKEPSLF NPNIRCVNLI TFEDGLRELS VIQSHLKVFE NEFTNLNLQF 

      3070       3080       3090       3100       3110       3120 
SDPNRQKLRI VESRPAESEL EANRAVIVKT KLFSATEQVR LSNNPAVVMG YLLDESAISE 

      3130       3140       3150       3160       3170       3180 
VKPTKVDFSN LLKDRFKIMQ FFPSVFTLIK MLTDESSDSE KSGLSPDLQQ VARYSNHLTL 

      3190       3200       3210       3220       3230       3240 
LSRMIQQAKP TVTVFYMLKG NLMNTEPTVA ELVSYGIKEG RFFRLSDTGV DASTYSVKYW 

      3250       3260       3270       3280       3290       3300 
KILHCISAIG CLPLSQADKS SLLMSFLNWR VNMDIRTSDC PLSSHEASIL SEFDGQVIAN 

      3310       3320       3330       3340       3350       3360 
ILASELSSVK RDSEREGLTD LLDYLNSPTE LLKKKPYLGT TCKFNTWGDS NRSGKFTYSS 

      3370       3380       3390       3400       3410       3420 
RSGESIGIFI AGKLHIHLSS ESVALLCETE RQVLSWMSKR RTEVITKEQH QLFLSLLPQS 

      3430       3440       3450       3460       3470       3480 
HECLQKHKDG SALSVIPDSS NPRLLKFVPL KKGLAVVKIK KQILTVKKQV VFDAESEPRL 

      3490       3500       3510       3520       3530       3540 
QWGHGCLSIV YDETDTQTTY HENLLKVKHL VDCSTDRKKL LPQSVFSDSK VVLSRIKFKT 

      3550       3560       3570       3580       3590       3600 
ELLLNSLTLL HCFLKHAPSD AIMEVESKSS LLHKYLKSGG VRQRNTEVLF REKLNKVVIK 

      3610       3620       3630       3640       3650       3660 
DNLEQGVEEE IEFCNNLTKT VSENPLPLSC WSEVQNYIED IGFNNVLVNI DRNTVKSELL 

      3670       3680       3690       3700       3710       3720 
WKFTLDTNVS TTSTIKDVRT LVSYVSTETI PKFLLAFLLY EEVLMNLINQ CKAVKELINS 

      3730       3740       3750       3760       3770       3780 
TGLSDLELES LLTLCAFYFQ SECSKRDGPR CSFAALLSLI HEDWQRIGKN ILVRANNELG 

      3790       3800       3810       3820       3830       3840 
DVSLKVNIVL VPLKDMSKPK SERVVMARRS LNHALSLMFL DEMSLPELKS LSVNCKMGNF 

      3850       3860       3870       3880       3890       3900 
EGQECFEFTI LKDNSARLDY NKLIDHCVDM EKKREAVRAV EDLILMLTGR AVKPSAVTQF 

      3910       3920       3930       3940 
VHGDEQCQEQ ISLDDLMAND TVTDFPDREA EALKTGNLGF NWDSD 

« Hide

References

[1]"Sequence determination of the Crimean-Congo hemorrhagic fever virus L segment."
Kinsella E., Martin S.G., Grolla A., Czub M., Feldmann H., Flick R.
Virology 321:23-28(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses."
Frias-Staheli N., Giannakopoulos N.V., Kikkert M., Taylor S.L., Bridgen A., Paragas J., Richt J.A., Rowland R.R., Schmaljohn C.S., Lenschow D.J., Snijder E.J., Garcia-Sastre A., Virgin H.W.
Cell Host Microbe 2:404-416(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF CYS-40 AND HIS-151.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY389361 Genomic DNA. Translation: AAR25663.2.
RefSeqYP_325663.1. NC_005301.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3PHUX-ray2.20A/B1-217[»]
3PHWX-ray2.00A/C/E/G1-183[»]
3PHXX-ray1.60A1-183[»]
3ZNHX-ray2.30A1-183[»]
ProteinModelPortalQ6TQR6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60307N.

Protein family/group databases

MEROPSC87.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2943075.

Family and domain databases

InterProIPR003323. OTU.
IPR015843. RNA-dir_pol_nairovirus.
IPR007099. RNA-dir_pol_NSvirus.
IPR007322. RNA_pol_bunyavir.
[Graphical view]
PfamPF04196. Bunya_RdRp. 1 hit.
PF02338. OTU. 1 hit.
[Graphical view]
PIRSFPIRSF036900. RdRPol_NRV. 1 hit.
PROSITEPS50802. OTU. 1 hit.
PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ6TQR6.

Entry information

Entry nameL_CCHFI
AccessionPrimary (citable) accession number: Q6TQR6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references