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Q6TPH1

- PPA23_ARATH

UniProt

Q6TPH1 - PPA23_ARATH

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Protein
Purple acid phosphatase 23
Gene
PAP23, AT3, At4g13700, F18A5.90
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Acid phosphatase activity with ATP, ADP, dATP, pyrophosphate, polyphosphate, phosphoserine and phosphothreonine. Low or no activity with phosphotyrosine, AMP and phytate.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 iron ion per subunit.1 Publication
Binds 1 manganese ion per subunit.1 Publication

pH dependencei

Optimum pH is 5-6.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi194 – 1941Iron Inferred
Metal bindingi221 – 2211Iron Inferred
Metal bindingi221 – 2211Manganese Inferred
Metal bindingi224 – 2241Iron Inferred
Metal bindingi278 – 2781Manganese Inferred
Binding sitei278 – 2781Substrate By similarity
Metal bindingi360 – 3601Manganese Inferred
Active sitei370 – 3701Proton donor By similarity
Metal bindingi397 – 3971Manganese Inferred
Metal bindingi399 – 3991Iron Inferred

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT4G13700-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 23 (EC:3.1.3.2)
Gene namesi
Name:PAP23
Synonyms:AT3
Ordered Locus Names:At4g13700
ORF Names:F18A5.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G13700.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 458439Purple acid phosphatase 23
PRO_0000372826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi59 – 591N-linked (GlcNAc...) Reviewed prediction
Glycosylationi121 – 1211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi136 – 1361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi200 – 2001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi331 – 3311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi409 – 4091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi455 – 4551N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Specifically expressed in flowers.1 Publication

Developmental stagei

First observed in the floral apical meristem (FAP). In flowers, observed in petals and anthers, particularly in anther filaments.1 Publication

Gene expression databases

GenevestigatoriQ6TPH1.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi3702.AT4G13700.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ6TPH1.
SMRiQ6TPH1. Positions 47-432.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni397 – 3993Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238330.
InParanoidiQ6TPH1.
PhylomeDBiQ6TPH1.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6TPH1-1 [UniParc]FASTAAdd to Basket

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MTLLIMITLT SISLLLAAAE TIPTTLDGPF KPLTRRFEPS LRRGSDDLPM    50
DHPRLRKRNV SSDFPEQIAL ALSTPTSMWV SWVTGDAIVG KDVKPLDPSS 100
IASEVWYGKE KGNYMLKKKG NATVYSQLYP SDGLLNYTSG IIHHVLIDGL 150
EPETRYYYRC GDSSVPAMSE EISFETLPLP SKDAYPHRIA FVGDLGLTSN 200
TTTTIDHLME NDPSLVIIVG DLTYANQYRT IGGKGVPCFS CSFPDAPIRE 250
TYQPRWDAWG RFMEPLTSKV PTMVIEGNHE IEPQASGITF KSYSERFAVP 300
ASESGSNSNL YYSFDAGGVH FVMLGAYVDY NNTGLQYAWL KEDLSKVDRA 350
VTPWLVATMH PPWYNSYSSH YQEFECMRQE MEELLYQYRV DIVFAGHVHA 400
YERMNRIYNY TLDPCGPVYI TIGDGGNIEK VDVDFADDPG KCHSSYDLFF 450
FNSLNLSN 458
Length:458
Mass (Da):51,551
Last modified:May 5, 2009 - v2
Checksum:i3FE6A789CBDF7A72
GO

Sequence cautioni

The sequence CAB36834.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAB78412.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti310 – 3101L → F in AAQ93685. 1 Publication
Sequence conflicti310 – 3101L → F in AEE83314. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY390530 mRNA. Translation: AAQ93685.1.
AL035528 Genomic DNA. Translation: CAB36834.1. Sequence problems.
AL161537 Genomic DNA. Translation: CAB78412.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83314.1.
PIRiT05239.
RefSeqiNP_193106.3. NM_117444.3.
UniGeneiAt.33346.

Genome annotation databases

EnsemblPlantsiAT4G13700.1; AT4G13700.1; AT4G13700.
GeneIDi827004.
KEGGiath:AT4G13700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY390530 mRNA. Translation: AAQ93685.1 .
AL035528 Genomic DNA. Translation: CAB36834.1 . Sequence problems.
AL161537 Genomic DNA. Translation: CAB78412.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE83314.1 .
PIRi T05239.
RefSeqi NP_193106.3. NM_117444.3.
UniGenei At.33346.

3D structure databases

ProteinModelPortali Q6TPH1.
SMRi Q6TPH1. Positions 47-432.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G13700.1-P.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G13700.1 ; AT4G13700.1 ; AT4G13700 .
GeneIDi 827004.
KEGGi ath:AT4G13700.

Organism-specific databases

TAIRi AT4G13700.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000238330.
InParanoidi Q6TPH1.
PhylomeDBi Q6TPH1.

Enzyme and pathway databases

BioCyci ARA:AT4G13700-MONOMER.

Miscellaneous databases

PROi Q6TPH1.

Gene expression databases

Genevestigatori Q6TPH1.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION, SEQUENCE REVISION.
    Strain: cv. Columbia.
  4. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPPA23_ARATH
AccessioniPrimary (citable) accession number: Q6TPH1
Secondary accession number(s): Q9SVP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: June 11, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi