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Protein

Purple acid phosphatase 23

Gene

PAP23

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acid phosphatase activity with ATP, ADP, dATP, pyrophosphate, polyphosphate, phosphoserine and phosphothreonine. Low or no activity with phosphotyrosine, AMP and phytate.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cation1 PublicationNote: Binds 1 Fe cation per subunit.1 Publication
  • Mn2+1 PublicationNote: Binds 1 Mn2+ ion per subunit.1 Publication

pH dependencei

Optimum pH is 5-6.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi194IronCurated1
Metal bindingi221IronCurated1
Metal bindingi221ManganeseCurated1
Metal bindingi224IronCurated1
Metal bindingi278ManganeseCurated1
Binding sitei278SubstrateBy similarity1
Metal bindingi360ManganeseCurated1
Active sitei370Proton donorBy similarity1
Metal bindingi397ManganeseCurated1
Metal bindingi399IronCurated1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.2. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 23 (EC:3.1.3.2)
Gene namesi
Name:PAP23
Synonyms:AT3
Ordered Locus Names:At4g13700
ORF Names:F18A5.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G13700.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000037282620 – 458Purple acid phosphatase 23Add BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...)Sequence analysis1
Glycosylationi121N-linked (GlcNAc...)Sequence analysis1
Glycosylationi136N-linked (GlcNAc...)Sequence analysis1
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi331N-linked (GlcNAc...)Sequence analysis1
Glycosylationi409N-linked (GlcNAc...)Sequence analysis1
Glycosylationi455N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6TPH1.

Expressioni

Tissue specificityi

Specifically expressed in flowers.1 Publication

Developmental stagei

First observed in the floral apical meristem (FAP). In flowers, observed in petals and anthers, particularly in anther filaments.1 Publication

Gene expression databases

GenevisibleiQ6TPH1. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G13700.1.

Structurei

3D structure databases

ProteinModelPortaliQ6TPH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni397 – 399Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1378. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000238330.
InParanoidiQ6TPH1.
OrthoDBiEOG093608JS.
PhylomeDBiQ6TPH1.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16656. Pur_ac_phosph_N. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6TPH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLIMITLT SISLLLAAAE TIPTTLDGPF KPLTRRFEPS LRRGSDDLPM
60 70 80 90 100
DHPRLRKRNV SSDFPEQIAL ALSTPTSMWV SWVTGDAIVG KDVKPLDPSS
110 120 130 140 150
IASEVWYGKE KGNYMLKKKG NATVYSQLYP SDGLLNYTSG IIHHVLIDGL
160 170 180 190 200
EPETRYYYRC GDSSVPAMSE EISFETLPLP SKDAYPHRIA FVGDLGLTSN
210 220 230 240 250
TTTTIDHLME NDPSLVIIVG DLTYANQYRT IGGKGVPCFS CSFPDAPIRE
260 270 280 290 300
TYQPRWDAWG RFMEPLTSKV PTMVIEGNHE IEPQASGITF KSYSERFAVP
310 320 330 340 350
ASESGSNSNL YYSFDAGGVH FVMLGAYVDY NNTGLQYAWL KEDLSKVDRA
360 370 380 390 400
VTPWLVATMH PPWYNSYSSH YQEFECMRQE MEELLYQYRV DIVFAGHVHA
410 420 430 440 450
YERMNRIYNY TLDPCGPVYI TIGDGGNIEK VDVDFADDPG KCHSSYDLFF

FNSLNLSN
Length:458
Mass (Da):51,551
Last modified:May 5, 2009 - v2
Checksum:i3FE6A789CBDF7A72
GO

Sequence cautioni

The sequence CAB36834 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB78412 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti310L → F in AAQ93685 (PubMed:16244908).Curated1
Sequence conflicti310L → F in AEE83314 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390530 mRNA. Translation: AAQ93685.1.
AL035528 Genomic DNA. Translation: CAB36834.1. Sequence problems.
AL161537 Genomic DNA. Translation: CAB78412.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83314.1.
PIRiT05239.
RefSeqiNP_193106.3. NM_117444.3.
UniGeneiAt.33346.

Genome annotation databases

EnsemblPlantsiAT4G13700.1; AT4G13700.1; AT4G13700.
GeneIDi827004.
GrameneiAT4G13700.1; AT4G13700.1; AT4G13700.
KEGGiath:AT4G13700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390530 mRNA. Translation: AAQ93685.1.
AL035528 Genomic DNA. Translation: CAB36834.1. Sequence problems.
AL161537 Genomic DNA. Translation: CAB78412.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83314.1.
PIRiT05239.
RefSeqiNP_193106.3. NM_117444.3.
UniGeneiAt.33346.

3D structure databases

ProteinModelPortaliQ6TPH1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G13700.1.

Proteomic databases

PaxDbiQ6TPH1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G13700.1; AT4G13700.1; AT4G13700.
GeneIDi827004.
GrameneiAT4G13700.1; AT4G13700.1; AT4G13700.
KEGGiath:AT4G13700.

Organism-specific databases

TAIRiAT4G13700.

Phylogenomic databases

eggNOGiKOG1378. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000238330.
InParanoidiQ6TPH1.
OrthoDBiEOG093608JS.
PhylomeDBiQ6TPH1.

Enzyme and pathway databases

BRENDAi3.1.3.2. 399.

Miscellaneous databases

PROiQ6TPH1.

Gene expression databases

GenevisibleiQ6TPH1. AT.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16656. Pur_ac_phosph_N. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPA23_ARATH
AccessioniPrimary (citable) accession number: Q6TPH1
Secondary accession number(s): Q9SVP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.