Q6TPH1 (PPA23_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 55.
History...
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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Purple acid phosphatase 23 EC=3.1.3.2 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Reference proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 458 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Acid phosphatase activity with ATP, ADP, dATP, pyrophosphate, polyphosphate, phosphoserine and phosphothreonine. Low or no activity with phosphotyrosine, AMP and phytate. Ref.1 |
| Catalytic activity | A phosphate monoester + H2O = an alcohol + phosphate. |
| Cofactor | Binds 1 iron ion per subunit. Ref.1 Binds 1 manganese ion per subunit. Ref.1 |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Secreted By similarity. |
| Tissue specificity | Specifically expressed in flowers. Ref.1 |
| Developmental stage | First observed in the floral apical meristem (FAP). In flowers, observed in petals and anthers, particularly in anther filaments. Ref.1 |
| Sequence similarities | Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. |
| Biophysicochemical properties | pH dependence: Optimum pH is 5-6. Ref.1 |
| Sequence caution | The sequence CAB36834.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB78412.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Secreted |
| Domain | Signal |
| Ligand | Iron Metal-binding Zinc |
| Molecular function | Hydrolase |
| PTM | Glycoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Cellular_component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | acid phosphatase activity Inferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | Potential | ||||||
| Chain | 20 – 458 | 439 | Purple acid phosphatase 23 | PRO_0000372826 | |||||
Regions | |||||||||
| Region | 397 – 399 | 3 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 370 | 1 | Proton donor By similarity | ||||||
| Metal binding | 194 | 1 | Iron Probable | ||||||
| Metal binding | 221 | 1 | Iron Probable | ||||||
| Metal binding | 221 | 1 | Manganese Probable | ||||||
| Metal binding | 224 | 1 | Iron Probable | ||||||
| Metal binding | 278 | 1 | Manganese Probable | ||||||
| Metal binding | 360 | 1 | Manganese Probable | ||||||
| Metal binding | 397 | 1 | Manganese Probable | ||||||
| Metal binding | 399 | 1 | Iron Probable | ||||||
| Binding site | 278 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 59 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 121 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 136 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 200 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 331 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 409 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 455 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 310 | 1 | L → F in AAQ93685. Ref.1 | ||||||
| Sequence conflict | 310 | 1 | L → F in AEE83314. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, BIOPHYSICOCHEMICAL PROPERTIES, COFACTORS, TISSUE SPECIFICITY. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.  McCombie W.R.Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION. Strain: cv. Columbia. |
| [4] | "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY390530 mRNA. Translation: AAQ93685.1. AL035528 Genomic DNA. Translation: CAB36834.1. Sequence problems. AL161537 Genomic DNA. Translation: CAB78412.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE83314.1. |
| IPI | IPI00541176. |
| PIR | T05239. |
| RefSeq | NP_193106.3. NM_117444.3. |
| UniGene | At.33346. |
3D structure databases | |
| HSSP | HSSP built from PDB template 4KBP based on UniProtKB P80366. |
| ProteinModelPortal | Q6TPH1. |
| SMR | Q6TPH1. Positions 47-432. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 3702.AT4G13700.1-P. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 827004. |
| KEGG | ath:AT4G13700. |
Organism-specific databases | |
| TAIR | At4g13700. |
Phylogenomic databases | |
| eggNOG | COG1409. |
| HOGENOM | HOG000238330. |
| InParanoid | Q6TPH1. |
| PhylomeDB | Q6TPH1. |
| ProtClustDB | CLSN2925562. |
Gene expression databases | |
| Genevestigator | Q6TPH1. |
Family and domain databases | |
| Gene3D | 2.60.40.380. 1 hit. |
| InterPro | IPR004843. Metallo_PEstase_dom. IPR008963. Purple_acid_Pase-like_N. IPR015914. Purple_acid_Pase_N. [Graphical view] |
| Pfam | PF00149. Metallophos. 1 hit. [Graphical view] |
| SUPFAM | SSF49363. Purple_Pase_N. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | PPA23_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q6TPH1 Secondary accession number(s): Q9SVP2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |