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Protein

Chitinase-3-like protein 1

Gene

CHI3L1

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201Chitooligosaccharide
Binding sitei241 – 2411Chitooligosaccharide
Binding sitei330 – 3301Chitooligosaccharide

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial

Keywords - Biological processi

Apoptosis, Inflammatory response

Keywords - Ligandi

Lectin

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase-3-like protein 1
Alternative name(s):
Secretory glycoprotein of 40 kDa
Signal-processing protein
Gene namesi
Name:CHI3L1
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Chitinase-3-like protein 1PRO_0000077055Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi5 ↔ 30
Glycosylationi39 – 391N-linked (GlcNAc...)2 Publications
Disulfide bondi278 ↔ 342
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Detected in mammary gland.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
361
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi9 – 135Combined sources
Helixi16 – 183Combined sources
Helixi22 – 243Combined sources
Turni27 – 293Combined sources
Beta strandi31 – 4111Combined sources
Beta strandi44 – 463Combined sources
Helixi52 – 6514Combined sources
Beta strandi70 – 767Combined sources
Turni77 – 793Combined sources
Helixi82 – 898Combined sources
Helixi92 – 10918Combined sources
Beta strandi112 – 1176Combined sources
Helixi123 – 1253Combined sources
Helixi126 – 14419Combined sources
Turni145 – 1473Combined sources
Beta strandi152 – 1576Combined sources
Helixi161 – 1677Combined sources
Helixi170 – 1767Combined sources
Beta strandi178 – 1825Combined sources
Beta strandi192 – 1943Combined sources
Beta strandi205 – 2073Combined sources
Beta strandi211 – 2144Combined sources
Helixi215 – 22410Combined sources
Helixi229 – 2313Combined sources
Beta strandi232 – 24817Combined sources
Beta strandi255 – 2595Combined sources
Turni264 – 2663Combined sources
Beta strandi271 – 2733Combined sources
Helixi274 – 2807Combined sources
Turni281 – 2833Combined sources
Beta strandi285 – 2895Combined sources
Turni290 – 2934Combined sources
Beta strandi294 – 2996Combined sources
Beta strandi302 – 3054Combined sources
Helixi309 – 32113Combined sources
Beta strandi325 – 3306Combined sources
Helixi332 – 3343Combined sources
Beta strandi337 – 3393Combined sources
Beta strandi341 – 3444Combined sources
Helixi349 – 35911Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SR0X-ray3.05A1-361[»]
1ZBKX-ray2.90A1-360[»]
1ZL1X-ray3.50A1-360[»]
2DPEX-ray2.07A1-361[»]
2DSUX-ray2.20A1-361[»]
2DSVX-ray2.54A1-361[»]
2DSWX-ray2.80A1-361[»]
2FDMX-ray3.00A1-360[»]
2G41X-ray3.00A1-361[»]
2G8ZX-ray2.50A1-361[»]
2PI6X-ray1.65A1-361[»]
ProteinModelPortaliQ6TMG6.
SMRiQ6TMG6. Positions 1-361.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6TMG6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 502Chitooligosaccharide binding
Regioni76 – 794Chitooligosaccharide binding
Regioni183 – 1864Chitooligosaccharide binding
Regioni302 – 31615Important for AKT1 activation and IL8 productionBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 18 family.Curated

Phylogenomic databases

HOVERGENiHBG011684.

Family and domain databases

Gene3Di3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR028538. CHI3L1.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11177:SF202. PTHR11177:SF202. 1 hit.
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6TMG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
YKLICYYTSW SQYREGDGSC FPDAIDPFLC THVIYSFANI SNNEIDTWEW
60 70 80 90 100
NDVTLYDTLN TLKNRNPKLK TLLSVGGWNF GPERFSAIAS KTQSRRTFIK
110 120 130 140 150
SVPPFLRTHG FDGLDLAWLY PGRRDKRHLT TLVKEMKAEF IREAQAGTEQ
160 170 180 190 200
LLLSAAVSAG KIAIDRGYDI AQISRHLDFI SLLTYDFHGA WRQTVGHHSP
210 220 230 240 250
LFAGNEDASS RFSNADYAVS YMLRLGAPAN KLVMGIPTFG RSFTLASSKT
260 270 280 290 300
DVGAPVSGPG VPGRFTKEKG ILAYYEICDF LHGATTHRFR DQQVPYATKG
310 320 330 340 350
NQWVAYDDQE SVKNKARYLK NRQLAGAMVW ALDLDDFRGT FCGQNLTFPL
360
TSAVKDVLAE V
Length:361
Mass (Da):40,563
Last modified:July 5, 2004 - v1
Checksum:i8642AF873DC5EE3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY392761 mRNA. Translation: AAQ94054.1.
UniGeneiOar.1069.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY392761 mRNA. Translation: AAQ94054.1.
UniGeneiOar.1069.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SR0X-ray3.05A1-361[»]
1ZBKX-ray2.90A1-360[»]
1ZL1X-ray3.50A1-360[»]
2DPEX-ray2.07A1-361[»]
2DSUX-ray2.20A1-361[»]
2DSVX-ray2.54A1-361[»]
2DSWX-ray2.80A1-361[»]
2FDMX-ray3.00A1-360[»]
2G41X-ray3.00A1-361[»]
2G8ZX-ray2.50A1-361[»]
2PI6X-ray1.65A1-361[»]
ProteinModelPortaliQ6TMG6.
SMRiQ6TMG6. Positions 1-361.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG011684.

Miscellaneous databases

EvolutionaryTraceiQ6TMG6.

Family and domain databases

Gene3Di3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR028538. CHI3L1.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11177:SF202. PTHR11177:SF202. 1 hit.
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCH3L1_SHEEP
AccessioniPrimary (citable) accession number: Q6TMG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 5, 2004
Last modified: November 11, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although it belongs to the glycosyl hydrolase 18 family, Leu-119 is present instead of the conserved Glu which is an active site residue. Therefore this protein lacks chitinase activity.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.