Reviewed, UniProtKB/Swiss-Prot Q6TMG5 (NEMO_RAT)

Names and origin

Protein names

Recommended name:
    NF-kappa-B essential modulator
      Short name=NEMO
Alternative name(s):
    NF-kappa-B essential modifier
    Inhibitor of nuclear factor kappa-B kinase subunit gamma
      Short name=IkB kinase subunit gamma
      Short name=I-kappa-B kinase gamma
      Short name=IKK-gamma
      Short name=IKKG
    IkB kinase-associated protein 1
      Short name=IKKAP1

Gene names

Name:	Ikbkg
Synonyms:	Nemo

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity By similarity.
Subunit structure	Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Interacts with COPS3, CYLD, NALP2, TRPC4AP and LRDD. Interacts with ATM; the complex is exported from the nucleus. Interacts with TRAF6. Interacts with TANK; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG By similarity. Interacts with ZFAND5 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Contains 1 C2HC-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
PTM	Disulfide bond Phosphoprotein
Gene Ontology (GO)
Biological process	NF-kappaB import into nucleus Ref.1 Traceable author statement. Source: RGD positive regulation of NF-kappaB transcription factor activity Ref.1 Traceable author statement. Source: RGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	IkappaB kinase complex Ref.1 Inferred from physical interaction. Source: RGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein heterodimerization activity Inferred from physical interaction. Source: RGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	NF-kappa-B essential modulator		PRO_0000269197
Regions
	Domain	315 – 336	22	Leucine-zipper Potential
	Zinc finger	389 – 410	22	C2HC-type
	Region	44 – 111	68	Interaction with CHUK/IKBKB By similarity
	Coiled coil	49 – 343	295	Potential
Amino acid modifications
	Modified residue	380	1	Phosphoserine By similarity
	Disulfide bond	54		Interchain By similarity
	Disulfide bond	340		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6TMG5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6D5D96B08A4CCEC2	FASTA	412	48,066
	10 20 30 40 50 60 MSRHLWKNQL SEMVQPSGGP AEDQDMLGEE SSLGKPAMLH LPSEQGTPET LQRCLEENQE 70 80 90 100 110 120 LRDAIRQSNQ MLRERCEELL HFQVSQREEK EFLMCKFQEA RKLVERLSLE KLDLRRQREQ 130 140 150 160 170 180 ALEDLEHLKK CQQQMAEDKA SVKAQVTSLL GELQESQSRL EAATKERQTL EGRIRAVSEQ 190 200 210 220 230 240 VRQLESEREV LQQQHSVQVD QLRMQNQSVE AALRMERQAA SEEKRKLAQL QAAYHQLFQD 250 260 270 280 290 300 YDSHIKSSKG MQLEDLRQQL QQAEEALVAK QELIDKLKEE AEQHKIVMET VPVLKAQADI 310 320 330 340 350 360 YKADFQAERH AREKLVERKE LLQEQLEQLQ REFNKLKVGC HESARIEDMR KRHVETSQPP 370 380 390 400 410 LLPAPAHHSF HLALSNQRRS PPEEPPDFCC PKCQYQAPDM DTLQIHVMEC IE

References

[1]

"Two carboxyl-terminal activation regions of Epstein-Barr virus latent membrane protein 1 activate NF-kappaB through distinct signaling pathways in fibroblast cell lines." Saito N., Courtois G., Chiba A., Yamamoto N., Nitta T., Hironaka N., Rowe M., Yamamoto N., Yamaoka S. J. Biol. Chem. 278:46565-46575(2003) [PubMed: 12968033] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY392762 mRNA. Translation: AAQ94056.1.
IPI	IPI00400576.
RefSeq	NP_954534.1.
UniGene	Rn.214715
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6TMG5.
Genome annotation databases
Ensembl	ENSRNOT00000056275; ENSRNOP00000053114; ENSRNOG00000037237; Rattus norvegicus. [Genome view]
GeneID	309295.
KEGG	rno:309295.
Organism-specific databases
CTD	309295.
RGD	735223. Ikbkg.
Phylogenomic databases
HOVERGEN	Q6TMG5.
Gene expression databases
Genevestigator	Q6TMG5.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	660548.

Entry information

Entry name

NEMO_RAT

Accession

Primary (citable) accession number: Q6TMG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)