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Protein

Rho GTPase-activating protein 27

Gene

Arhgap27

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: HGNC
  • SH3 domain binding Source: HGNC

GO - Biological processi

  • positive regulation of GTPase activity Source: HGNC
  • receptor-mediated endocytosis Source: HGNC
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 27
Alternative name(s):
CIN85-associated multi-domain-containing Rho GTPase-activating protein 1
Rho-type GTPase-activating protein 27
Gene namesi
Name:Arhgap27
Synonyms:Camgap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi735202. Arhgap27.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • intracellular Source: HGNC
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 869869Rho GTPase-activating protein 27PRO_0000317580Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei155 – 1551PhosphoserineCombined sources
Modified residuei215 – 2151PhosphoserineBy similarity
Modified residuei249 – 2491PhosphoserineBy similarity
Modified residuei350 – 3501PhosphoserineBy similarity
Modified residuei459 – 4591PhosphoserineBy similarity
Modified residuei462 – 4621PhosphoserineBy similarity
Modified residuei464 – 4641PhosphothreonineBy similarity
Modified residuei469 – 4691PhosphoserineBy similarity
Modified residuei632 – 6321PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6TLK4.
PRIDEiQ6TLK4.

PTM databases

iPTMnetiQ6TLK4.
PhosphoSiteiQ6TLK4.

Interactioni

Subunit structurei

Interacts with SH3KBP1/CIN85.1 Publication

GO - Molecular functioni

  • SH3 domain binding Source: HGNC

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034607.

Structurei

3D structure databases

ProteinModelPortaliQ6TLK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 6962SH3PROSITE-ProRule annotationAdd
BLAST
Domaini246 – 28035WW 1PROSITE-ProRule annotationAdd
BLAST
Domaini299 – 33335WW 2PROSITE-ProRule annotationAdd
BLAST
Domaini414 – 44734WW 3PROSITE-ProRule annotationAdd
BLAST
Domaini477 – 593117PHPROSITE-ProRule annotationAdd
BLAST
Domaini677 – 866190Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 3 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1450. Eukaryota.
KOG4269. Eukaryota.
ENOG410ZP6T. LUCA.
HOGENOMiHOG000294167.
HOVERGENiHBG005328.
InParanoidiQ6TLK4.
PhylomeDBiQ6TLK4.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 2 hits.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00456. WW. 3 hits.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51045. SSF51045. 3 hits.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6TLK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADVEGDVY VLVEHPFEYT GKDGRLIAIQ PNERCRLLRR STEHWWHVRR
60 70 80 90 100
EPGGRPFYLP AQYVRELPAL GDPVPAPQPS VLQQRPTVPE PLAYDYRFVS
110 120 130 140 150
TPVGADGSSA EPRGRASSLC GPARQRTSGQ RNSLAPGGPA CLYLRPAAPV
160 170 180 190 200
RPAQSLDDLA RGGTAPPAGL LGSAGHFKAS SVAGSWVCPR PLARSDSENV
210 220 230 240 250
YEAIPDLRCP PRAKSPKQVD EPPEPVYANV ERQPQVTSPR SAAAPPRLSP
260 270 280 290 300
VWETHTDAGT GRPYYYNPDT GVTTWESPFE APEGATSPTT SRASVGSGES
310 320 330 340 350
LETEWGQYWD EESGRVFFYN PLTGETVWED ETEELEDDPE EQLEMQPSLS
360 370 380 390 400
PRSPGQQRPP TPETDYPELL TSYPEEDYSP VGSFSDLGPT SPLVAPPGWS
410 420 430 440 450
CQITPEKQML YTNQFTQEQW VRLEDQEGKP YFYNPEDSSV QWELPQVPVP
460 470 480 490 500
APRSGRKSSQ DSDTPAQASP PEEKIKTLDK AGVLHRTKTV DKGKRLRKKH
510 520 530 540 550
WNASWTVLEG GVLTFFKDSK TSAASGLRQP SKLSTPEYTV ELRGASLSWA
560 570 580 590 600
PKDKSSKKNV LELRSRDGSE YLIQHDSEAI ISTWHKAIAE GIEELSADLP
610 620 630 640 650
QREEGEPSSA DFGSSERLGS WKEEDVRPNA ASPSLNPGSQ ESDLSRVRHK
660 670 680 690 700
LRKFLQRRPT LQSLREKGYI KDQVFGCALA QLCERERSPV PRFVQQCIRT
710 720 730 740 750
VEARGLDIDG LYRISGNLAT IQKLRYKVDH DERLDLDDGR WEDVHVITGA
760 770 780 790 800
LKLFFRELPE PLFPFSHFHQ FIAAIKLQDP AQRSRCVRDL VRTLPAPNHD
810 820 830 840 850
TLRLLIQHLC RVIEHGEQNR MSVQNVAIVF GPTLLRPEME EASMPMTMVF
860
QNQVVELILH QCADIFPPH
Length:869
Mass (Da):97,180
Last modified:July 5, 2004 - v1
Checksum:i59E7855A46B2ADE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY394725 mRNA. Translation: AAQ94494.1.
RefSeqiNP_942054.1. NM_198759.1.
UniGeneiRn.17895.

Genome annotation databases

GeneIDi303583.
KEGGirno:303583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY394725 mRNA. Translation: AAQ94494.1.
RefSeqiNP_942054.1. NM_198759.1.
UniGeneiRn.17895.

3D structure databases

ProteinModelPortaliQ6TLK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034607.

PTM databases

iPTMnetiQ6TLK4.
PhosphoSiteiQ6TLK4.

Proteomic databases

PaxDbiQ6TLK4.
PRIDEiQ6TLK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi303583.
KEGGirno:303583.

Organism-specific databases

CTDi201176.
RGDi735202. Arhgap27.

Phylogenomic databases

eggNOGiKOG1450. Eukaryota.
KOG4269. Eukaryota.
ENOG410ZP6T. LUCA.
HOGENOMiHOG000294167.
HOVERGENiHBG005328.
InParanoidiQ6TLK4.
PhylomeDBiQ6TLK4.

Miscellaneous databases

PROiQ6TLK4.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 2 hits.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00456. WW. 3 hits.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51045. SSF51045. 3 hits.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel Rho GTPase activating protein implicated in receptor-mediated endocytosis."
    Sakakibara T., Nemoto Y., Nukiwa T., Takeshima H.
    FEBS Lett. 566:294-300(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SH3KBP1.
    Strain: Sprague-Dawley.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155 AND SER-632, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRHG27_RAT
AccessioniPrimary (citable) accession number: Q6TLK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.