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Protein

Regucalcin

Gene

rgn

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Catalyzes a key step in ascorbic acid (vitamin C) biosynthesis. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca2+ signaling, and Ca2+-dependent cellular processes and enzyme activities (By similarity).By similarity

Catalytic activityi

D-glucono-1,5-lactone + H2O = D-gluconate.

Cofactori

Zn2+By similarity, Mn2+By similarity, Ca2+By similarity, Mg2+By similarityNote: Binds 1 divalent metal cation per subunit. Most active with Zn2+ and Mn2+ ions. The physiological cofactor is most likely Ca2+ or Mg2+.By similarity

Pathwayi: L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway

This protein is involved in step 3 of the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Regucalcin (rgn)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate, the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi18 – 181Divalent metal cationBy similarity
Binding sitei100 – 1001SubstrateBy similarity
Binding sitei102 – 1021SubstrateBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Metal bindingi150 – 1501Divalent metal cationBy similarity
Active sitei200 – 2001Proton donor/acceptorBy similarity
Metal bindingi200 – 2001Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ascorbate biosynthesis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00991; UER00938.

Names & Taxonomyi

Protein namesi
Recommended name:
Regucalcin
Short name:
RC
Alternative name(s):
Gluconolactonase (EC:3.1.1.17)
Short name:
GNL
Gene namesi
Name:rgnBy similarity
ORF Names:zgc:92078
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 6

Organism-specific databases

ZFINiZDB-GENE-040718-68. rgn.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295RegucalcinPRO_0000287685Add
BLAST

Proteomic databases

PaxDbiQ6TLF6.

Expressioni

Gene expression databases

BgeeiENSDARG00000032200.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000126252.

Structurei

3D structure databases

ProteinModelPortaliQ6TLF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SMP-30/CGR1 family.By similarity

Phylogenomic databases

eggNOGiKOG4499. Eukaryota.
COG3386. LUCA.
GeneTreeiENSGT00390000014995.
HOGENOMiHOG000220627.
HOVERGENiHBG004347.
InParanoidiQ6TLF6.
KOiK01053.
OMAiCARDGMD.
OrthoDBiEOG091G131G.
PhylomeDBiQ6TLF6.
TreeFamiTF323663.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR008367. Regucalcin.
IPR013658. SGL.
IPR005511. SMP-30.
[Graphical view]
PfamiPF08450. SGL. 1 hit.
[Graphical view]
PRINTSiPR01791. REGUCALCIN.
PR01790. SMP30FAMILY.

Sequencei

Sequence statusi: Complete.

Q6TLF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIKVECVI KEKNEVGESP VWEEKDSSLL YVDITGQKVS RWSSLTKQIE
60 70 80 90 100
SMNTEKLVGC VVPRQAGGYV IAEGTRFAFV DWVKRSITAV AEVNEKPNTR
110 120 130 140 150
FNDGKVDPAG RFFAGTMSMD MKPDVVDAAL YNLQPDHSVV RHFDQVHLSN
160 170 180 190 200
GLDWSLDHRV FYYIDSLAFM VEAFDYDIQT GGLSNRRTVY KMEKDEGIPD
210 220 230 240 250
GMCIDTEGKL WVACFNGGRV LRIDPQTGKR LQTVKLPAER ITSCCFGGKD
260 270 280 290
YSDLYITSAY IGMDAEALAK QPEAGCTFKV TGLGVKGIPP YSYTG
Length:295
Mass (Da):32,809
Last modified:July 5, 2004 - v1
Checksum:i1FF5C6DD40D6B68D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY394949 mRNA. Translation: AAQ94576.1.
BC075882 mRNA. Translation: AAH75882.1.
RefSeqiNP_991309.1. NM_205746.1.
UniGeneiDr.152116.

Genome annotation databases

EnsembliENSDART00000162588; ENSDARP00000130159; ENSDARG00000098645.
GeneIDi403070.
KEGGidre:403070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY394949 mRNA. Translation: AAQ94576.1.
BC075882 mRNA. Translation: AAH75882.1.
RefSeqiNP_991309.1. NM_205746.1.
UniGeneiDr.152116.

3D structure databases

ProteinModelPortaliQ6TLF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000126252.

Proteomic databases

PaxDbiQ6TLF6.

Protocols and materials databases

DNASUi403070.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000162588; ENSDARP00000130159; ENSDARG00000098645.
GeneIDi403070.
KEGGidre:403070.

Organism-specific databases

CTDi9104.
ZFINiZDB-GENE-040718-68. rgn.

Phylogenomic databases

eggNOGiKOG4499. Eukaryota.
COG3386. LUCA.
GeneTreeiENSGT00390000014995.
HOGENOMiHOG000220627.
HOVERGENiHBG004347.
InParanoidiQ6TLF6.
KOiK01053.
OMAiCARDGMD.
OrthoDBiEOG091G131G.
PhylomeDBiQ6TLF6.
TreeFamiTF323663.

Enzyme and pathway databases

UniPathwayiUPA00991; UER00938.

Miscellaneous databases

PROiQ6TLF6.

Gene expression databases

BgeeiENSDARG00000032200.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR008367. Regucalcin.
IPR013658. SGL.
IPR005511. SMP-30.
[Graphical view]
PfamiPF08450. SGL. 1 hit.
[Graphical view]
PRINTSiPR01791. REGUCALCIN.
PR01790. SMP30FAMILY.
ProtoNetiSearch...

Entry informationi

Entry nameiRGN_DANRE
AccessioniPrimary (citable) accession number: Q6TLF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.