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Protein

Regucalcin

Gene

rgn

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Catalyzes a key step in ascorbic acid (vitamin C) biosynthesis. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca2+ signaling, and Ca2+-dependent cellular processes and enzyme activities (By similarity).By similarity

Catalytic activityi

D-glucono-1,5-lactone + H2O = D-gluconate.

Cofactori

Zn2+By similarity, Mn2+By similarity, Ca2+By similarity, Mg2+By similarityNote: Binds 1 divalent metal cation per subunit. Most active with Zn2+ and Mn2+ ions. The physiological cofactor is most likely Ca2+ or Mg2+.By similarity

Pathwayi: L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway

This protein is involved in step 3 of the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Regucalcin (rgn)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate, the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi18Divalent metal cationBy similarity1
Binding sitei100SubstrateBy similarity1
Binding sitei102SubstrateBy similarity1
Binding sitei120SubstrateBy similarity1
Metal bindingi150Divalent metal cationBy similarity1
Active sitei200Proton donor/acceptorBy similarity1
Metal bindingi200Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAscorbate biosynthesis
LigandCalcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00991; UER00938.

Names & Taxonomyi

Protein namesi
Recommended name:
Regucalcin
Short name:
RC
Alternative name(s):
Gluconolactonase (EC:3.1.1.17)
Short name:
GNL
Gene namesi
Name:rgnBy similarity
ORF Names:zgc:92078
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 6

Organism-specific databases

ZFINiZDB-GENE-040718-68. rgn.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002876851 – 295RegucalcinAdd BLAST295

Proteomic databases

PaxDbiQ6TLF6.

Expressioni

Gene expression databases

BgeeiENSDARG00000098645.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000126252.

Structurei

3D structure databases

ProteinModelPortaliQ6TLF6.
SMRiQ6TLF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SMP-30/CGR1 family.By similarity

Phylogenomic databases

eggNOGiKOG4499. Eukaryota.
COG3386. LUCA.
GeneTreeiENSGT00390000014995.
HOGENOMiHOG000220627.
HOVERGENiHBG004347.
InParanoidiQ6TLF6.
KOiK01053.
OMAiCRWDSLT.
OrthoDBiEOG091G131G.
PhylomeDBiQ6TLF6.
TreeFamiTF323663.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiView protein in InterPro
IPR011042. 6-blade_b-propeller_TolB-like.
IPR008367. Regucalcin.
IPR013658. SGL.
IPR005511. SMP-30.
PfamiView protein in Pfam
PF08450. SGL. 1 hit.
PRINTSiPR01791. REGUCALCIN.
PR01790. SMP30FAMILY.

Sequencei

Sequence statusi: Complete.

Q6TLF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIKVECVI KEKNEVGESP VWEEKDSSLL YVDITGQKVS RWSSLTKQIE
60 70 80 90 100
SMNTEKLVGC VVPRQAGGYV IAEGTRFAFV DWVKRSITAV AEVNEKPNTR
110 120 130 140 150
FNDGKVDPAG RFFAGTMSMD MKPDVVDAAL YNLQPDHSVV RHFDQVHLSN
160 170 180 190 200
GLDWSLDHRV FYYIDSLAFM VEAFDYDIQT GGLSNRRTVY KMEKDEGIPD
210 220 230 240 250
GMCIDTEGKL WVACFNGGRV LRIDPQTGKR LQTVKLPAER ITSCCFGGKD
260 270 280 290
YSDLYITSAY IGMDAEALAK QPEAGCTFKV TGLGVKGIPP YSYTG
Length:295
Mass (Da):32,809
Last modified:July 5, 2004 - v1
Checksum:i1FF5C6DD40D6B68D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY394949 mRNA. Translation: AAQ94576.1.
BC075882 mRNA. Translation: AAH75882.1.
RefSeqiNP_991309.1. NM_205746.1.
UniGeneiDr.152116.

Genome annotation databases

EnsembliENSDART00000162588; ENSDARP00000130159; ENSDARG00000098645.
GeneIDi403070.
KEGGidre:403070.

Similar proteinsi

Entry informationi

Entry nameiRGN_DANRE
AccessioniPrimary (citable) accession number: Q6TLF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: July 5, 2004
Last modified: August 30, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families