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Q6TGS6 (SYYC_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tyrosine--tRNA ligase, cytoplasmic
EC=6.1.1.1
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS
Gene names
Name:yars
OrganismDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.

Catalytic activity

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtyrosyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

tyrosine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 529529Tyrosine--tRNA ligase, cytoplasmic
PRO_0000239693

Regions

Domain365 – 469105tRNA-binding
Motif44 – 529"HIGH" region By similarity
Motif222 – 2265"KMSKS" region By similarity

Sites

Binding site391Tyrosine By similarity
Binding site1661Tyrosine By similarity
Binding site1701Tyrosine By similarity
Binding site1731Tyrosine By similarity
Binding site1881Tyrosine By similarity

Experimental info

Sequence conflict3511V → A in AAQ97863. Ref.1
Sequence conflict3511V → A in CAK04282. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6TGS6 [UniParc].

Last modified June 27, 2006. Version 2.
Checksum: A3264E3E39D362F4

FASTA52959,534
        10         20         30         40         50         60 
MGEQLSPDEK FQLITRNLQE VLGEERLKEI LKERELKVYW GTATTGKPHV AYFVPMSKIA 

        70         80         90        100        110        120 
DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVKYYEQV IKAMLESIGV PLDKLKFVKG 

       130        140        150        160        170        180 
TDYQLSREYT LDVYRLSSMV TEHDAKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD 

       190        200        210        220        230        240 
AQFGGVDQRK IFTLAEKYLP SLGYTKRSHL MNPMVPGLTG SKMSSSEEES KIDLLDKNQD 

       250        260        270        280        290        300 
VKKKLKKAFC EPGNVENNGV LSFVKHVLFP LHSEFVIKRD PKFGGDKVYT DFEEVEKDFA 

       310        320        330        340        350        360 
AEQIHPGDLK ASVELALNKL LDPIRKKFES PELKKLTSSA YPEPSKNKGG VKGNPKQTTD 

       370        380        390        400        410        420 
DDEVIPSRLD IRVGKVISVE KHPDADSLYL EKIDVGEEQP RTVVSGLVAY ITEEQLQDRL 

       430        440        450        460        470        480 
VVLLCNLKPQ KMRGIESQAM VLCASIEGEP RKVEPLDPPE GSAAGDRVYV EGYESGKPDD 

       490        500        510        520 
ELKPKKKVFE KLQVDLKISG EFVAQWKEQN LMTKLGRITC KTLKGGNIS

« Hide

References

[1]"Hematopoietic gene expression profile in zebrafish kidney marrow."
Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y., Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X., Look A.T., Chen Z.
Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004) [PubMed: 15520368] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney marrow.
[2]The Danio rerio sequencing project at the Sanger Institute
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tuebingen.
[3]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY398430 mRNA. Translation: AAQ97863.1.
BX547927 Genomic DNA. Translation: CAK04282.1.
BX000986 Genomic DNA. Translation: CAK04465.1.
BC076558 mRNA. Translation: AAH76558.1.
IPIIPI00497960.
RefSeqNP_958473.1. NM_201316.1.
UniGeneDr.81609.

3D structure databases

ProteinModelPortalQ6TGS6.
SMRQ6TGS6. Positions 6-342, 362-529.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ6TGS6.

Proteomic databases

PRIDEQ6TGS6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000052126; ENSDARP00000052125; ENSDARG00000035913.
ENSDART00000124491; ENSDARP00000108800; ENSDARG00000035913.
GeneID368235.
KEGGdre:368235.
NMPDRfig|7955.3.peg.10397.

Organism-specific databases

CTD8565.
ZFINZDB-GENE-030425-2. yars.

Phylogenomic databases

GeneTreeENSGT00550000074927.
HOGENOMHBG330667.
HOVERGENHBG080113.
InParanoidQ6TGS6.
OrthoDBEOG4RV2R7.
PhylomeDBQ6TGS6.

Gene expression databases

ArrayExpressQ6TGS6.
BgeeQ6TGS6.

Family and domain databases

InterProIPR002305. aa-tRNA-synth_Ic.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR002307. Tyr-tRNA-synth.
IPR023617. Tyr-tRNA-synth_arc-type.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01866.
PANTHERPTHR11946:SF8. Tyr-tRNA_synth. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01040. TRNASYNTHTYR.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00234. TyrS. 1 hit.
PROSITEPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYYC_DANRE
AccessionPrimary (citable) accession number: Q6TGS6
Secondary accession number(s): Q6DFZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: January 25, 2012
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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