Reviewed, UniProtKB/Swiss-Prot Q6TGS6 (SYYC_DANRE)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.1
Alternative name(s):
    Tyrosyl--tRNA ligase
      Short name=TyrRS

Gene names

Name:

yars

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Tyrosyl-tRNA synthetase, cytoplasmic		PRO_0000239693
Regions
	Domain	365 – 469	105	tRNA-binding
	Motif	44 – 52	9	"HIGH" region By similarity
	Motif	222 – 226	5	"KMSKS" region By similarity
Sites
	Binding site	39	1	Tyrosine By similarity
	Binding site	166	1	Tyrosine By similarity
	Binding site	170	1	Tyrosine By similarity
	Binding site	173	1	Tyrosine By similarity
	Binding site	188	1	Tyrosine By similarity
Experimental info
	Sequence conflict	351	1	V → A in AAQ97863. Ref.1
	Sequence conflict	351	1	V → A in CAK04282. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6TGS6-1 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: A3264E3E39D362F4	FASTA	529	59,534
	10 20 30 40 50 60 MGEQLSPDEK FQLITRNLQE VLGEERLKEI LKERELKVYW GTATTGKPHV AYFVPMSKIA 70 80 90 100 110 120 DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVKYYEQV IKAMLESIGV PLDKLKFVKG 130 140 150 160 170 180 TDYQLSREYT LDVYRLSSMV TEHDAKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD 190 200 210 220 230 240 AQFGGVDQRK IFTLAEKYLP SLGYTKRSHL MNPMVPGLTG SKMSSSEEES KIDLLDKNQD 250 260 270 280 290 300 VKKKLKKAFC EPGNVENNGV LSFVKHVLFP LHSEFVIKRD PKFGGDKVYT DFEEVEKDFA 310 320 330 340 350 360 AEQIHPGDLK ASVELALNKL LDPIRKKFES PELKKLTSSA YPEPSKNKGG VKGNPKQTTD 370 380 390 400 410 420 DDEVIPSRLD IRVGKVISVE KHPDADSLYL EKIDVGEEQP RTVVSGLVAY ITEEQLQDRL 430 440 450 460 470 480 VVLLCNLKPQ KMRGIESQAM VLCASIEGEP RKVEPLDPPE GSAAGDRVYV EGYESGKPDD 490 500 510 520 ELKPKKKVFE KLQVDLKISG EFVAQWKEQN LMTKLGRITC KTLKGGNIS

References

[1]	"Hematopoietic gene expression profile in zebrafish kidney marrow." Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y., Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X., Look A.T., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004) [PubMed: 15520368] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney marrow.
[2]	The Danio rerio sequencing project at the Sanger Institute Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY398430 mRNA. Translation: AAQ97863.1. BX547927 Genomic DNA. Translation: CAK04282.1. BX000986 Genomic DNA. Translation: CAK04465.1. BC076558 mRNA. Translation: AAH76558.1.
IPI	IPI00497960.
RefSeq	NP_958473.1.
UniGene	Dr.81609
3D structure databases
SMR	Q6TGS6. Positions 6-342, 362-529.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6TGS6.
Proteomic databases
PRIDE	Q6TGS6.
Genome annotation databases
Ensembl	ENSDART00000052126; ENSDARP00000052125; ENSDARG00000035913; Danio rerio. [Genome view]
GeneID	368235.
KEGG	dre:368235.
NMPDR	fig|7955.3.peg.10397.
Organism-specific databases
CTD	368235.
ZFIN	ZDB-GENE-030425-2. yars.
Phylogenomic databases
HOGENOM	Q6TGS6.
HOVERGEN	Q6TGS6.
Enzyme and pathway databases
BRENDA	6.1.1.1. 96826.
Gene expression databases
ArrayExpress	Q6TGS6.
Bgee	Q6TGS6.
Family and domain databases
InterPro	IPR002305. aa-tRNA-synth_Ib. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA_bd. IPR015624. Tyr-tRNA-synth_Ib_arc/euk. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF8. Tyr-tRNA_synth. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYYC_DANRE

Accession

Primary (citable) accession number: Q6TGS6
Secondary accession number(s): Q6DFZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	September 1, 2009
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project