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Q6TGQ8

- OXLA_BOTMO

UniProt

Q6TGQ8 - OXLA_BOTMO

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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Bothrops moojeni (Lance-headed viper) (Caissaca)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (L-Phe>L-Tyr>L-Trp>L-Leu), thus producing hydrogen peroxide that may contribute to the toxicity of the venom. Exhibits diverse biological activities, such as edema, apoptosis of tumor cell lines, antibacterial activities against both Gram-positive and Gram-negative bacteria, as well as induction of platelet aggregation. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions. Unlike other snake venom L-amino acid oxidases, does not induce hemorrhage. It may also induce hemolysis, and have antiparasitic activities.2 Publications

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FAD.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771FADBy similarity
Binding sitei96 – 961SubstrateBy similarity
Binding sitei229 – 2291SubstrateBy similarity
Binding sitei267 – 2671FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei378 – 3781SubstrateBy similarity
Binding sitei463 – 4631FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 502FADBy similarity
Nucleotide bindingi69 – 702FADBy similarity
Nucleotide bindingi93 – 964FADBy similarity
Nucleotide bindingi470 – 4756FADBy similarity
Nucleotide bindingi470 – 4712SubstrateBy similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. defense response to Gram-negative bacterium Source: UniProtKB
  3. defense response to Gram-positive bacterium Source: UniProtKB
  4. hemolysis in other organism Source: UniProtKB-KW
  5. modulation of apoptotic process in other organism Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation activating toxin, Toxin

Keywords - Biological processi

Apoptosis, Cytolysis, Hemolysis

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
BmooLAAO-I
Short name:
LAAO
Short name:
LAO
OrganismiBothrops moojeni (Lance-headed viper) (Caissaca)
Taxonomic identifieri98334 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 6›61 Publication
Chaini7 – ›478›472L-amino-acid oxidasePRO_0000273565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi16 ↔ 179By similarity
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi337 ↔ 418By similarity

Post-translational modificationi

N-glycosylated (Probable). The enzymatic activity is not affected by deglycosylation.1 PublicationCurated

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer; non-covalently linked.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ6TGQ8.
SMRiQ6TGQ8. Positions 9-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG005729.

Family and domain databases

InterProiIPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6TGQ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
RKAPCCADDR NPLEECFRET DYEEFLEIAK NGLSTTSNPK RVVIVGAGMS
60 70 80 90 100
GLSAAYVLAN AGHQVTVLEA SERAGGRVKT YRNEKEGWYA NLGPMRLPEK
110 120 130 140 150
HRIVREYIRK FDLQLNEFSQ ENENAWYFIK NIRKRVGEVN KDPGVLEYPV
160 170 180 190 200
KPSEVGKSAG QLYEESLQKA VEELRRTNCS YMLNKYDTYS TKEYLLKEGN
210 220 230 240 250
LSPGAVDMIG DLLNEDSGYY VSFIESLKHD DIFAYEKRFD EIVGGMDKLP
260 270 280 290 300
TSMYQAIQEK VHLNARVIKI QQDVKEVTVT YQTSEKETLS VTADYVIVCT
310 320 330 340 350
TSRAARRIKF EPPLPPKKAH ALRSVHYRSG TKIFLTCTKK FWEDDGIHGG
360 370 380 390 400
KSTTDLPSRF IYYPNHNFPN GVGVIIAYGI GDDANYFQAL DFEDCGDIVI
410 420 430 440 450
NDLSLIHQLP KEEIQAICRP SMIQRWSLDK YAMGGITTFT PYQFQHFSEA
460 470
LTAPVDRIYF AGEYTAQAHG WIDSTIKW
Length:478
Mass (Da):54,437
Last modified:July 5, 2004 - v1
Checksum:i555C159B029FB9CB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti34 – 352ST → KS AA sequence (PubMed:17320169)Curated
Non-terminal residuei478 – 4781

Mass spectrometryi

Molecular mass is 64889 Da from positions 7 - ?. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY398692 mRNA. Translation: AAR31183.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY398692 mRNA. Translation: AAR31183.1 .

3D structure databases

ProteinModelPortali Q6TGQ8.
SMRi Q6TGQ8. Positions 9-477.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG005729.

Family and domain databases

InterProi IPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
PRINTSi PR00757. AMINEOXDASEF.
ProtoNeti Search...

Publicationsi

  1. "Molecular approaches for structural characterization of Bothrops L-amino acid oxidases with antiprotozoal activity: cDNA cloning, comparative sequence analysis, and molecular modeling."
    Franca S.C., Kashima S., Roberto P.G., Marins M., Ticli F.K., Pereira J.O., Astolfi-Filho S., Stabeli R.G., Magro A.J., Fontes M.R., Sampaio S.V., Soares A.M.
    Biochem. Biophys. Res. Commun. 355:302-306(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom and Venom gland.
  2. Cited for: PROTEIN SEQUENCE OF 7-46, FUNCTION, SUBUNIT, GLYCOSYLATION, MASS SPECTROMETRY, CIRCULAR DICHROISM.
    Tissue: Venom.
  3. "Bothrops moojeni venom kills Leishmania spp. with hydrogen peroxide generated by its L-amino acid oxidase."
    Tempone A.G., Andrade H.F. Jr., Spencer P.J., Lourenco C.O., Rogero J.R., Nascimento N.
    Biochem. Biophys. Res. Commun. 280:620-624(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
    Tissue: Venom.

Entry informationi

Entry nameiOXLA_BOTMO
AccessioniPrimary (citable) accession number: Q6TGQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2004
Last modified: October 1, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Has parasiticidal activities against and leishmania, as a result of enzyme-catalyzed hydrogen peroxide production (PubMed:11162565, PubMed:17292326).2 Publications

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3