Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

MGMT

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5ZincBy similarity1
Metal bindingi24ZincBy similarity1
Metal bindingi29ZincBy similarity1
Metal bindingi89ZincBy similarity1
Binding sitei99DNA; via amide nitrogenBy similarity1
Binding sitei118DNABy similarity1
Binding sitei119DNA; via amide nitrogenBy similarity1
Binding sitei127DNABy similarity1
Binding sitei132DNABy similarity1
Active sitei149Nucleophile; methyl group acceptorPROSITE-ProRule annotation1
Binding sitei155DNA; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:MGMT
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393571 – 216Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6TDU1.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000035481.

Structurei

3D structure databases

ProteinModelPortaliQ6TDU1.
SMRiQ6TDU1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

eggNOGiKOG4062. Eukaryota.
COG0350. LUCA.
HOGENOMiHOG000244137.
HOVERGENiHBG001146.
InParanoidiQ6TDU1.
KOiK00567.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR036217. MethylDNA_cys_MeTrfase_DNAb.
IPR008332. MethylG_MeTrfase_N.
IPR036631. MGMT_N_sf.
IPR036388. WH-like_DNA-bd_sf.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6TDU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKACKMRYK TMDSPLGKIE ISGCAQGLHE IRLHDRKTPD PGPAEAPAPP
60 70 80 90 100
ELLGCPEEMT EPLVQCVAWL DAYFREPSVL PGLPLPAIHH PIFQRGSFTA
110 120 130 140 150
RVLRKLLELV KFGDTVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH
160 170 180 190 200
RVVCSSGAMG NYTGGLATKE WLLAHEGRLA GKPTYPGGSY LAGTRRGALG
210
GTPSPHPAGR DGCVRE
Length:216
Mass (Da):23,052
Last modified:July 5, 2004 - v1
Checksum:i4A56DD567F93532F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY423553 mRNA. Translation: AAR84186.1.
RefSeqiNP_001003376.1. NM_001003376.1.
UniGeneiCfa.3907.

Genome annotation databases

GeneIDi442978.
KEGGicfa:442978.

Similar proteinsi

Entry informationi

Entry nameiMGMT_CANLF
AccessioniPrimary (citable) accession number: Q6TDU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: July 5, 2004
Last modified: February 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome