Reviewed, UniProtKB/Swiss-Prot Q6T893 (GLPE_ACTPL)

Names and origin

Protein names

Recommended name:
    Thiosulfate sulfurtransferase glpE
    EC=2.8.1.1

Gene names

Name:

glpE

Organism

Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)

Taxonomic identifier

715 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide By similarity. HAMAP MF_01009
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP MF_01009
Subcellular location	Cytoplasm By similarity HAMAP MF_01009.
Sequence similarities	Belongs to the glpE family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiosulfate sulfurtransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Thiosulfate sulfurtransferase glpE HAMAP MF_01009		PRO_0000200547
Regions
	Domain	18 – 106	89	Rhodanese
Sites
	Active site	66	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6T893-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E31DA8D75B3A4C16	FASTA	108	12,403
	10 20 30 40 50 60 MSETFTEISP HQAWELIENE GATLADIRDG RRYAYSHPQD AFHLTNESYG RFLDEVDYEE 70 80 90 100 PVIVMCYHGV SSRNTAQFLV EQGFDRVYSV KGGFDGWERS GLPIETAY

References

[1]	"Two TonB systems in Actinobacillus pleuropneumoniae: their roles in iron acquisition and virulence." Beddek A.J., Sheehan B.J., Bosse J.T., Rycroft A.N., Kroll J.S., Langford P.R. Infect. Immun. 72:701-708(2004) [PubMed: 14742511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY428647 Genomic DNA. Translation: AAR95692.1.
3D structure databases
SMR	Q6T893. Positions 1-107.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.8.1.1. 257522.
Family and domain databases
HAMAP	MF_01009. Thiosulf_sulfurtr. [Tree]
InterPro	IPR001763. Rhodanese-like. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPE_ACTPL

Accession

Primary (citable) accession number: Q6T893

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)