Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bitiscystatin

Gene
N/A
Organism
Bitis gabonica (Gaboon adder) (Gaboon viper)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits various C1 cysteine proteases including cathepsin L, papain and cathepsin B. This protein has no toxic activity and its function in the venom is unknown. It may play a role as housekeeping or regulatory protein (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei29 – 291Reactive siteBy similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI25.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Bitiscystatin
Short name:
Cystatin
OrganismiBitis gabonica (Gaboon adder) (Gaboon viper)
Taxonomic identifieri8694 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeBitis

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 140114BitiscystatinPRO_0000319471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi91 ↔ 107By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6T6T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 129101CystatinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi73 – 775Secondary area of contact

Sequence similaritiesi

Belongs to the cystatin family.Curated
Contains 1 cystatin domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG009556.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Prot_inh_cystat.
IPR020381. Prot_inh_cystat_cons-reg.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
ProDomiPD001231. Prot_inh_cystat_cons-reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6T6T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSRLLVAAP HCLLLLLLLP SALPALKVGG LYPRDVMDPE VQEAAAFAVE
60 70 80 90 100
NYNAQSTNDN YFKARRIVEA QSQVVSGVKY YLKMELAKTT CKKIAGKPKL
110 120 130 140
YQEIQNCNLP PENQQEEITC HFEVWSRPWL QKTVLTKDEL
Length:140
Mass (Da):15,900
Last modified:July 4, 2004 - v1
Checksum:i771DA8853F7D7CE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY430403 mRNA. Translation: AAR24527.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY430403 mRNA. Translation: AAR24527.1.

3D structure databases

ProteinModelPortaliQ6T6T4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI25.012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG009556.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Prot_inh_cystat.
IPR020381. Prot_inh_cystat_cons-reg.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
ProDomiPD001231. Prot_inh_cystat_cons-reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bitis gabonica (Gaboon viper) snake venom gland: toward a catalog for the full-length transcripts (cDNA) and proteins."
    Francischetti I.M.B., My-Pham V., Harrison J., Garfield M.K., Ribeiro J.M.C.
    Gene 337:55-69(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Venom gland.
  2. "Snake venomics of Bitis gabonica gabonica. Protein family composition, subunit organization of venom toxins, and characterization of dimeric disintegrins bitisgabonin-1 and bitisgabonin-2."
    Calvete J.J., Marcinkiewicz C., Sanz L.
    J. Proteome Res. 6:326-336(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-41, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Venom.

Entry informationi

Entry nameiCYT_BITGA
AccessioniPrimary (citable) accession number: Q6T6T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 25, 2008
Last sequence update: July 4, 2004
Last modified: January 6, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.