ID   S22AP_HUMAN             Reviewed;         547 AA.
AC   Q6T423; A4FU27;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 2.
DT   27-MAR-2024, entry version 142.
DE   RecName: Full=Solute carrier family 22 member 25;
DE   AltName: Full=Organic anion transporter UST6;
GN   Name=SLC22A25 {ECO:0000312|HGNC:HGNC:32935};
GN   Synonyms=UST6 {ECO:0000303|PubMed:15054140};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAR84082.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND VARIANT VAL-486.
RC   TISSUE=Liver {ECO:0000269|PubMed:15054140};
RX   PubMed=15054140; DOI=10.1152/physiolgenomics.00014.2004;
RA   Eraly S.A., Monte J.C., Nigam S.K.;
RT   "Novel slc22 transporter homologs in fly, worm, and human clarify the
RT   phylogeny of organic anion and cation transporters.";
RL   Physiol. Genomics 18:12-24(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16554811; DOI=10.1038/nature04632;
RA   Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA   Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA   Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA   Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA   Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA   Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT   "Human chromosome 11 DNA sequence and analysis including novel gene
RT   identification.";
RL   Nature 440:497-500(2006).
RN   [3] {ECO:0000305, ECO:0000312|EMBL:AAI01317.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS GLY-250; THR-300 AND
RP   VAL-486.
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed exclusively in liver in both embryo and
CC       adult. {ECO:0000269|PubMed:15054140}.
CC   -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC       Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR   EMBL; AY437532; AAR84082.1; -; mRNA.
DR   EMBL; AP001880; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP003420; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC101316; AAI01317.1; -; mRNA.
DR   CCDS; CCDS31592.1; -.
DR   RefSeq; NP_955384.3; NM_199352.3.
DR   RefSeq; XP_016873176.1; XM_017017687.1.
DR   AlphaFoldDB; Q6T423; -.
DR   SMR; Q6T423; -.
DR   BioGRID; 132365; 2.
DR   STRING; 9606.ENSP00000307443; -.
DR   TCDB; 2.A.1.19.33; the major facilitator superfamily (mfs).
DR   GlyCosmos; Q6T423; 2 sites, No reported glycans.
DR   GlyGen; Q6T423; 2 sites.
DR   iPTMnet; Q6T423; -.
DR   PhosphoSitePlus; Q6T423; -.
DR   BioMuta; SLC22A25; -.
DR   DMDM; 296452891; -.
DR   MassIVE; Q6T423; -.
DR   PaxDb; 9606-ENSP00000307443; -.
DR   PeptideAtlas; Q6T423; -.
DR   Antibodypedia; 51271; 48 antibodies from 11 providers.
DR   DNASU; 387601; -.
DR   Ensembl; ENST00000306494.11; ENSP00000307443.6; ENSG00000196600.13.
DR   GeneID; 387601; -.
DR   KEGG; hsa:387601; -.
DR   MANE-Select; ENST00000306494.11; ENSP00000307443.6; NM_199352.6; NP_955384.3.
DR   UCSC; uc001nwr.2; human.
DR   AGR; HGNC:32935; -.
DR   CTD; 387601; -.
DR   DisGeNET; 387601; -.
DR   GeneCards; SLC22A25; -.
DR   HGNC; HGNC:32935; SLC22A25.
DR   HPA; ENSG00000196600; Tissue enriched (liver).
DR   MIM; 610792; gene.
DR   neXtProt; NX_Q6T423; -.
DR   OpenTargets; ENSG00000196600; -.
DR   PharmGKB; PA162403567; -.
DR   VEuPathDB; HostDB:ENSG00000196600; -.
DR   eggNOG; KOG0255; Eukaryota.
DR   GeneTree; ENSGT00940000167143; -.
DR   HOGENOM; CLU_001265_33_3_1; -.
DR   InParanoid; Q6T423; -.
DR   OMA; VEWITHR; -.
DR   OrthoDB; 2088942at2759; -.
DR   PhylomeDB; Q6T423; -.
DR   TreeFam; TF315847; -.
DR   PathwayCommons; Q6T423; -.
DR   BioGRID-ORCS; 387601; 42 hits in 1143 CRISPR screens.
DR   ChiTaRS; SLC22A25; human.
DR   GeneWiki; SLC22A25; -.
DR   GenomeRNAi; 387601; -.
DR   Pharos; Q6T423; Tdark.
DR   PRO; PR:Q6T423; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   RNAct; Q6T423; Protein.
DR   Bgee; ENSG00000196600; Expressed in right lobe of liver and 28 other cell types or tissues.
DR   ExpressionAtlas; Q6T423; baseline and differential.
DR   GO; GO:0098590; C:plasma membrane region; IEA:UniProt.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0015711; P:organic anion transport; IBA:GO_Central.
DR   CDD; cd17374; MFS_OAT; 1.
DR   Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   PANTHER; PTHR24064; SOLUTE CARRIER FAMILY 22 MEMBER; 1.
DR   PANTHER; PTHR24064:SF217; SOLUTE CARRIER FAMILY 22 MEMBER 25; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..547
FT                   /note="Solute carrier family 22 member 25"
FT                   /id="PRO_0000328926"
FT   TOPO_DOM        1..9
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        10..30
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        31..145
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        167..177
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        199..204
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        205..225
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        226..234
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        235..255
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        256..259
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        281..349
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        350..370
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        371..377
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        378..398
FT                   /note="Helical; Name=8"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        399..406
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..427
FT                   /note="Helical; Name=9"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        428..434
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        435..455
FT                   /note="Helical; Name=10"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        456..470
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        471..491
FT                   /note="Helical; Name=11"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        492..494
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        495..515
FT                   /note="Helical; Name=12"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        516..547
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        56
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        102
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VARIANT         89
FT                   /note="R -> C (in dbSNP:rs35722529)"
FT                   /id="VAR_057783"
FT   VARIANT         250
FT                   /note="S -> G (in dbSNP:rs11231409)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_042576"
FT   VARIANT         300
FT                   /note="R -> T (in dbSNP:rs11231397)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_042577"
FT   VARIANT         448
FT                   /note="G -> A (in dbSNP:rs17157907)"
FT                   /id="VAR_057784"
FT   VARIANT         486
FT                   /note="M -> V (in dbSNP:rs6591771)"
FT                   /evidence="ECO:0000269|PubMed:15054140,
FT                   ECO:0000269|PubMed:15489334"
FT                   /id="VAR_060287"
SQ   SEQUENCE   547 AA;  61008 MW;  A6964B2363FE68A0 CRC64;
     MAFQDLLDQV GGLGRFQILQ MVFLIMFNVI VYHQTQLENF AAFILDHRCW VHILDNDTIP
     DNDPGTLSQD ALLRISIPFD SNLRPEKCRR FVHPQWKLIH LNGTFPNTSE PDTEPCVDGW
     VYDQSSFPST IVTKWDLVCE SQPLNSVAKF LFMAGMMVGG NLYGHLSDRF GRKFVLRWSY
     LQLAIVGTCA AFAPTILVYC SLRFLAGAAT FSIIVNTVLL IVEWITHQFC AMALTLTLCA
     ASIGHITLGS LAFVIRDQCI LQLVMSAPCF VFFLFSRWLA ESARWLIINN KPEEGLKELR
     KAAHRNGMKN AEDILTMEVL KSTMKQELEA AQKKHSLCEL LRIPNICKRI CFLSFVRFAS
     TIPFWGLTLH LQHLGNNVFL LQTLFGAVTL LANCVAPWAL NHMSRRLSQM LLMFLLATCL
     LAIIFVPQEM QTLRVVLATL GVGAASLGIT CSTAQENELI PSIIRGRATG ITGNFANIGG
     ALASLMMILS IYSRPLPWII YGVFAILSGL VVLLLPETRN QPLLDSIQDV ENEGVNSLAA
     PQRSSVL
//