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Protein

1,4-alpha-glucan-branching enzyme

Gene

GBE1

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells.

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway:iglycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei354 – 3541NucleophileBy similarity
Active sitei409 – 4091Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Brancher enzyme
Glycogen-branching enzyme
Gene namesi
Name:GBE1
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712 Componenti: Unplaced

Pathology & Biotechi

Involvement in diseasei

Defects in GBE1 are the cause of glycogen storage disease IV (GSD-IV).

Keywords - Diseasei

Glycogen storage disease

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6996991,4-alpha-glucan-branching enzymePRO_0000188773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei170 – 1701PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Highest levels found in liver and muscle.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000025102.

Structurei

3D structure databases

ProteinModelPortaliQ6T308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG051734.
InParanoidiQ6T308.
KOiK00700.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6T308-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPVARGEC SEAALAAALA DVPELARLLE LDPYLKPFAL DFQRRYKKFN
60 70 80 90 100
ETLNNIGENE GGIDKFSRGY ESFGVHRCAD GGLYCKEWAP GAEGVFLTGD
110 120 130 140 150
FNDWNPFSYP YKKLDYGKWE LYIPPKQNKS QLVPHGSKLK VVIRSKSGEI
160 170 180 190 200
LYRISPWAKY VTREGENVNY DWTHWDPEHP YKFKHSRPKK PRGVRIYESH
210 220 230 240 250
VGISSYEGKI ASYKHFTYNV LPRIKDLGYN CIQMMAIMEH AYYASFGYQI
260 270 280 290 300
TSFFAASSRY GTPEELKELV DTAHSMGITV LLDVVHSHAS KNSEDGLNMF
310 320 330 340 350
DGTDSCYFHS GPRGNHDLWD SRLFIYSSWE VLRFLLSNIR WWLEEYGFDG
360 370 380 390 400
FRFDGVTSML YHHHGMGQAF SGDYHEYFGL QVDEDALIYL MLANHLVHTL
410 420 430 440 450
YPNSITIAED VSGMPALCSP ISQGGVGFDY RLAMAIPDKW IQLLKEFKDE
460 470 480 490 500
DWNMGNIVYT LTNRRYLEKC IAYAESHDQA LVGDKTLAFW LMDAEMYTNM
510 520 530 540 550
SVLTPFTPVI DRGIQLHKMI RLITHALGGE GYLNFMGNEF GHPEWLDFPR
560 570 580 590 600
KGNNESYHYA RRQFHLTDDD LLRYKFLNNF DRDMNKLEER CGWLSAPQAF
610 620 630 640 650
VSEKHEGNKI IAFERAGLVF IFNFHPSKSY TDYRVGTTLP GKFRIVLDTD
660 670 680 690
AAEYGGHQRL DHSTEFFSQP FKHNERPCSL LVYIPNRVGL ILQNVDMPN
Length:699
Mass (Da):80,564
Last modified:July 5, 2004 - v1
Checksum:i2B463AA38C5CCEA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY439007 mRNA. Translation: AAR13899.1.
RefSeqiNP_001009872.1. NM_001009872.1.

Genome annotation databases

GeneIDi493962.
KEGGifca:493962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY439007 mRNA. Translation: AAR13899.1.
RefSeqiNP_001009872.1. NM_001009872.1.

3D structure databases

ProteinModelPortaliQ6T308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000025102.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi493962.
KEGGifca:493962.

Organism-specific databases

CTDi2632.

Phylogenomic databases

HOVERGENiHBG051734.
InParanoidiQ6T308.
KOiK00700.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of feline glycogen storage disease type IV."
    Fyfe J.C., Hawkins M.G., Henthorn P.S.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISEASE.

Entry informationi

Entry nameiGLGB_FELCA
AccessioniPrimary (citable) accession number: Q6T308
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.