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Q6T308 (GLGB_FELCA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan-branching enzyme

EC=2.4.1.18
Alternative name(s):
Brancher enzyme
Glycogen-branching enzyme
Gene names
Name:GBE1
OrganismFelis catus (Cat) (Felis silvestris catus) [Reference proteome]
Taxonomic identifier9685 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis

Protein attributes

Sequence length699 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells.

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway

Glycan biosynthesis; glycogen biosynthesis.

Subunit structure

Monomer.

Tissue specificity

Highest levels found in liver and muscle.

Involvement in disease

Defects in GBE1 are the cause of glycogen storage disease IV (GSD-IV). Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6996991,4-alpha-glucan-branching enzyme
PRO_0000188773

Sites

Active site3541Nucleophile By similarity
Active site4091Proton donor By similarity

Amino acid modifications

Modified residue1701Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6T308 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 2B463AA38C5CCEA3

FASTA69980,564
        10         20         30         40         50         60 
MAAPVARGEC SEAALAAALA DVPELARLLE LDPYLKPFAL DFQRRYKKFN ETLNNIGENE 

        70         80         90        100        110        120 
GGIDKFSRGY ESFGVHRCAD GGLYCKEWAP GAEGVFLTGD FNDWNPFSYP YKKLDYGKWE 

       130        140        150        160        170        180 
LYIPPKQNKS QLVPHGSKLK VVIRSKSGEI LYRISPWAKY VTREGENVNY DWTHWDPEHP 

       190        200        210        220        230        240 
YKFKHSRPKK PRGVRIYESH VGISSYEGKI ASYKHFTYNV LPRIKDLGYN CIQMMAIMEH 

       250        260        270        280        290        300 
AYYASFGYQI TSFFAASSRY GTPEELKELV DTAHSMGITV LLDVVHSHAS KNSEDGLNMF 

       310        320        330        340        350        360 
DGTDSCYFHS GPRGNHDLWD SRLFIYSSWE VLRFLLSNIR WWLEEYGFDG FRFDGVTSML 

       370        380        390        400        410        420 
YHHHGMGQAF SGDYHEYFGL QVDEDALIYL MLANHLVHTL YPNSITIAED VSGMPALCSP 

       430        440        450        460        470        480 
ISQGGVGFDY RLAMAIPDKW IQLLKEFKDE DWNMGNIVYT LTNRRYLEKC IAYAESHDQA 

       490        500        510        520        530        540 
LVGDKTLAFW LMDAEMYTNM SVLTPFTPVI DRGIQLHKMI RLITHALGGE GYLNFMGNEF 

       550        560        570        580        590        600 
GHPEWLDFPR KGNNESYHYA RRQFHLTDDD LLRYKFLNNF DRDMNKLEER CGWLSAPQAF 

       610        620        630        640        650        660 
VSEKHEGNKI IAFERAGLVF IFNFHPSKSY TDYRVGTTLP GKFRIVLDTD AAEYGGHQRL 

       670        680        690 
DHSTEFFSQP FKHNERPCSL LVYIPNRVGL ILQNVDMPN 

« Hide

References

[1]"Molecular characterization of feline glycogen storage disease type IV."
Fyfe J.C., Hawkins M.G., Henthorn P.S.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISEASE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY439007 mRNA. Translation: AAR13899.1.
RefSeqNP_001009872.1. NM_001009872.1.

3D structure databases

ProteinModelPortalQ6T308.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID493962.
KEGGfca:493962.

Organism-specific databases

CTD2632.

Phylogenomic databases

HOVERGENHBG051734.
KOK00700.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_FELCA
AccessionPrimary (citable) accession number: Q6T308
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 5, 2004
Last modified: January 22, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries