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Protein

Isocitrate lyase

Gene

icl1

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (icl1)
  2. Malate synthase (AFUA_6G03540)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi168MagnesiumBy similarity1
Active sitei206Proton acceptorBy similarity1
Binding sitei243SubstrateBy similarity1
Binding sitei457SubstrateBy similarity1

GO - Molecular functioni

  • isocitrate lyase activity Source: AspGD
  • metal ion binding Source: UniProtKB-KW
  • methylisocitrate lyase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:icl1By similarity
Synonyms:icl1 Publication
ORF Names:AFUA_4G13510
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiFungiDB:Afu4g13510.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000687831 – 538Isocitrate lyaseAdd BLAST538

Proteomic databases

PRIDEiQ6T267.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi5085.CADAFUBP00006860.

Structurei

3D structure databases

ProteinModelPortaliQ6T267.
SMRiQ6T267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 99Substrate bindingBy similarity3
Regioni207 – 208Substrate bindingBy similarity2
Regioni423 – 427Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000238475.
InParanoidiQ6T267.
KOiK01637.
OMAiLEKDWAE.
OrthoDBiEOG092C19UX.

Family and domain databases

InterProiView protein in InterPro
IPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
PANTHERiPTHR21631. PTHR21631. 1 hit.
PfamiView protein in Pfam
PF00463. ICL. 1 hit.
PIRSFiPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiView protein in PROSITE
PS00161. ISOCITRATE_LYASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6T267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLEDEDQK YWDDVQAVKA WWKDSRWRYT KRPYTAEQIV AKRGNLKIHY
60 70 80 90 100
PSNDQSKKLW KILESNFEKK VASFTYGCLE PTMLTQMAKY LDTVYVSGWQ
110 120 130 140 150
SSSTASSTDE PSPDLADYPM NTVPNKVNQL FMAQLFHDRK QREERITTPK
160 170 180 190 200
DQRSKLPNID YLRPIIADAD TGHGGLTAVM KLTKLFIERG AAGIHIEDQA
210 220 230 240 250
PGTKKCGHMA GKVLVPISEH INRLVAIRAQ ADIMGTDLLA IARTDSEAAT
260 270 280 290 300
LITSTIDHRD HAFIVGSTNP NLQPLNDLML AGEQAGKTGE ELQAIEDQWI
310 320 330 340 350
AQAGLKLFDD AVVDTIKAGV HVNKDALIKE YLTAAKGKSN SEARAIAKGI
360 370 380 390 400
TGVDIYWDWD APRTREGYYR YQGGTQCAIN RAVAYAPFAD LIWMESKLPD
410 420 430 440 450
YAQAKEFADG VHAVWPEQKL AYNLSPSFNW KKAMPREEQE TYIKRLGALG
460 470 480 490 500
YAWQFITLAG LHTTALISDQ FARAYAKQGM RAYGELVQEP EMEQGVDVVT
510 520 530
HQKWSGANYV DNMLKMLTGG VSSTAAMGKG VTEDQFKH
Length:538
Mass (Da):60,244
Last modified:December 6, 2005 - v2
Checksum:i5FC535DE4C376D6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68 – 69EK → D (Ref. 1) Curated2
Sequence conflicti235G → GE in AAR15146 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442291 Genomic DNA. Translation: AAR15146.1.
AAHF01000005 Genomic DNA. Translation: EAL89442.1.
RefSeqiXP_751480.1. XM_746387.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00008149; CADAFUAP00008149; CADAFUAG00008149.
GeneIDi3509073.
KEGGiafm:AFUA_4G13510.

Similar proteinsi

Entry informationi

Entry nameiACEA_ASPFU
AccessioniPrimary (citable) accession number: Q6T267
Secondary accession number(s): Q4WQP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: December 6, 2005
Last modified: August 30, 2017
This is version 91 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families