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Protein

Mastermind-like protein 1

Gene

Maml1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.2 Publications

GO - Molecular functioni

GO - Biological processi

  • atrioventricular node cell development Source: BHF-UCL
  • myoblast differentiation Source: UniProtKB
  • Notch signaling pathway Source: UniProtKB
  • positive regulation of muscle cell differentiation Source: UniProtKB
  • positive regulation of myotube differentiation Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of transcription of Notch receptor target Source: MGI
  • protein phosphorylation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Notch signaling pathway, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2122947. NOTCH1 Intracellular Domain Regulates Transcription.
R-MMU-2197563. NOTCH2 intracellular domain regulates transcription.
R-MMU-350054. Notch-HLH transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Mastermind-like protein 1
Short name:
Mam-1
Gene namesi
Name:Maml1Imported
Synonyms:Kiaa0200Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1890504. Maml1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10201020Mastermind-like protein 1PRO_0000129494Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei127 – 1271PhosphoserineCombined sources
Modified residuei310 – 3101PhosphoserineCombined sources
Modified residuei321 – 3211PhosphoserineCombined sources
Modified residuei367 – 3671PhosphoserineBy similarity
Modified residuei827 – 8271N6-acetyllysineBy similarity
Modified residuei1019 – 10191PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6T264.
PaxDbiQ6T264.
PRIDEiQ6T264.

PTM databases

iPTMnetiQ6T264.
PhosphoSiteiQ6T264.

Expressioni

Tissue specificityi

At E9.5, strongly expressed in the telencephalon, first branchial arch, forelimb buds and somites. By E10.5, continuously expressed in brain and spinal cord. Also expressed in first and second branchial arches and limb buds. By E11.5, expression in CNS is weak but increases in mesodermal tissues. At E14.5, detected in epithelial cells in trachea, esophagus and proximal and distal tubules of the developing lungs.1 Publication

Gene expression databases

BgeeiQ6T264.
CleanExiMM_MAML1.
ExpressionAtlasiQ6T264. baseline and differential.
GenevisibleiQ6T264. MM.

Interactioni

Subunit structurei

Interacts (via N-terminus) with NOTCH1, NOTCH2, NOTCH3 and NOTCH4 (via ankyrin repeat region). Interacts (via N-terminus) with p53 (via DNA-binding region). Forms a DNA-binding complex with Notch proteins and RBPSUH/RBP-J kappa/CBF1. Also binds CREBBP/CBP and CDK8.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi222170. 5 interactions.
IntActiQ6T264. 1 interaction.
MINTiMINT-8375705.
STRINGi10090.ENSMUSP00000059210.

Structurei

3D structure databases

ProteinModelPortaliQ6T264.
SMRiQ6T264. Positions 16-70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9797Required for interaction with NOTCH proteins1 PublicationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi66 – 10237Ala-richSequence analysisAdd
BLAST
Compositional biasi642 – 65110Poly-Gln

Domaini

The C-terminal region is required for transcriptional activation.1 Publication

Sequence similaritiesi

Belongs to the mastermind family.Curated

Phylogenomic databases

eggNOGiENOG410IHPP. Eukaryota.
ENOG4111N9A. LUCA.
GeneTreeiENSGT00530000063317.
HOVERGENiHBG058017.
InParanoidiQ6T264.
KOiK06061.
OMAiQNPSWQH.
PhylomeDBiQ6T264.
TreeFamiTF332922.

Family and domain databases

InterProiIPR019082. Neuroggenic_mastermind-like_N.
[Graphical view]
PfamiPF09596. MamL-1. 1 hit.
[Graphical view]
SMARTiSM01275. MamL-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6T264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLPTCPMAE FALPRHSAVM ERLRRRIELC RRHHSTCEAR YEAVSPERLE
60 70 80 90 100
LERQHTFALH QRCIQAKAKR AGKHRQPPAA ATAPVAAPAP ASAPAAARLD
110 120 130 140 150
AADGPEHGRP VAHLHDTVKR SLDSAASPQN GDQPNGYGDL FPGHKKTRRE
160 170 180 190 200
APLGVSVSAN GLPPASPLGQ PDKPSGGDTL QTAGKHSLGL DPINKKCLAD
210 220 230 240 250
SGIHLNGGSN SSEPFPLSLS KELKQEPVDD LPCMIAGAGG SVAQSNLMPD
260 270 280 290 300
LNLNEQEWKE LIEELNRSVP DEDMKDLFTE DFEEKKDPEP PGSATQTPLA
310 320 330 340 350
QDINIKTEFS PAAFEQEQLG SPQVRAGSAG QTFLGASSAP VGTDSPSLGS
360 370 380 390 400
SQTLFHTTSQ PGVDNSSPNL MPASAQAQSA QRALTSVVLP SQGPGGASEL
410 420 430 440 450
SSAHQLQQIA AKQKREQMLQ NPQQAAPAPG PGQLATWQQA GPSHSPLDVP
460 470 480 490 500
YPMEKPASPS GYKQDFTNSK LLMMPGVNKS SPRPGGPYLQ PSHSNLLSHQ
510 520 530 540 550
SPSNLNQNPV NNQGSVLDYG NTKPLSHYKA DCGQGGPGSG QNKPALMAYL
560 570 580 590 600
PQQLPHLSNE QNSLFLMKPK SGNMPFRSLV PPGQEQNPSS VPVAAPAASV
610 620 630 640 650
GTQPTVSVAS THNSSPYLSS QQQAAVMKQH QLLLDQQKQR EQQQQQLQQQ
660 670 680 690 700
QFLQRQHLLA EQEKQQFQRH LTRPPPQYQD PTQSTFPQQV GQFTGPSAAV
710 720 730 740 750
PGMNNLGPSN SSCPRVFPQP GTLMSMGPGH APVSSLPSSS GQQDRGVAQF
760 770 780 790 800
TGSQSLPQNS LYGMASGLAQ IVAQPPPQAT STHAHIPRQT NVGQNASTSA
810 820 830 840 850
AYGQNSLGSA SLSQQHSKGT LPPGLTKPQV PRVSAAMGSQ NASWQHQGMP
860 870 880 890 900
NLSSQTSGNS SVNPFTAAPS FHIQQAHLKL AGQQFSQAMP SRPMAPLSSA
910 920 930 940 950
GAAGPMLPPV SAQQRNSAPA SAPPQAAPQQ GLPGLSPSGP ELGAFGQSPT
960 970 980 990 1000
SQMSGRPGLH CAQAYPVRTM GQELPFAYSG QPGSSGLSSV AGHTDLIDSL
1010 1020
LKNRTSEEWI NELDDLLGSQ
Length:1,020
Mass (Da):107,726
Last modified:July 5, 2005 - v2
Checksum:i637132736ECD3A78
GO

Sequence cautioni

The sequence AAH94599.2 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC39618.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC97898.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti574 – 5741M → T in AAH58658 (PubMed:15489334).Curated
Sequence conflicti643 – 6464Missing in AAS07633 (PubMed:15019995).Curated
Sequence conflicti643 – 6464Missing in BAC97898 (PubMed:14621295).Curated
Sequence conflicti675 – 6751P → S in AAS07633 (PubMed:15019995).Curated
Sequence conflicti769 – 7691A → P in BAC39618 (PubMed:16141072).Curated
Sequence conflicti769 – 7691A → T in AAS07633 (PubMed:15019995).Curated
Sequence conflicti770 – 7701Q → H in BAC39618 (PubMed:16141072).Curated
Sequence conflicti822 – 8221P → L in AAS07633 (PubMed:15019995).Curated
Sequence conflicti861 – 8611S → C in AAS07633 (PubMed:15019995).Curated
Sequence conflicti1003 – 10031N → K in BAC39618 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442299 mRNA. Translation: AAS07633.1.
AK129088 mRNA. Translation: BAC97898.1. Different initiation.
AL646002 Genomic DNA. Translation: CAI24683.2.
BC024668 mRNA. Translation: AAH24668.1.
BC058658 mRNA. Translation: AAH58658.1.
BC094599 mRNA. Translation: AAH94599.2. Different initiation.
AK086146 mRNA. Translation: BAC39618.1. Different initiation.
CCDSiCCDS24632.2.
RefSeqiNP_780543.2. NM_175334.3.
UniGeneiMm.51116.

Genome annotation databases

EnsembliENSMUST00000059458; ENSMUSP00000059210; ENSMUSG00000050567.
GeneIDi103806.
KEGGimmu:103806.
UCSCiuc007isd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442299 mRNA. Translation: AAS07633.1.
AK129088 mRNA. Translation: BAC97898.1. Different initiation.
AL646002 Genomic DNA. Translation: CAI24683.2.
BC024668 mRNA. Translation: AAH24668.1.
BC058658 mRNA. Translation: AAH58658.1.
BC094599 mRNA. Translation: AAH94599.2. Different initiation.
AK086146 mRNA. Translation: BAC39618.1. Different initiation.
CCDSiCCDS24632.2.
RefSeqiNP_780543.2. NM_175334.3.
UniGeneiMm.51116.

3D structure databases

ProteinModelPortaliQ6T264.
SMRiQ6T264. Positions 16-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222170. 5 interactions.
IntActiQ6T264. 1 interaction.
MINTiMINT-8375705.
STRINGi10090.ENSMUSP00000059210.

PTM databases

iPTMnetiQ6T264.
PhosphoSiteiQ6T264.

Proteomic databases

EPDiQ6T264.
PaxDbiQ6T264.
PRIDEiQ6T264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059458; ENSMUSP00000059210; ENSMUSG00000050567.
GeneIDi103806.
KEGGimmu:103806.
UCSCiuc007isd.2. mouse.

Organism-specific databases

CTDi9794.
MGIiMGI:1890504. Maml1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IHPP. Eukaryota.
ENOG4111N9A. LUCA.
GeneTreeiENSGT00530000063317.
HOVERGENiHBG058017.
InParanoidiQ6T264.
KOiK06061.
OMAiQNPSWQH.
PhylomeDBiQ6T264.
TreeFamiTF332922.

Enzyme and pathway databases

ReactomeiR-MMU-2122947. NOTCH1 Intracellular Domain Regulates Transcription.
R-MMU-2197563. NOTCH2 intracellular domain regulates transcription.
R-MMU-350054. Notch-HLH transcription pathway.

Miscellaneous databases

NextBioi356133.
PROiQ6T264.
SOURCEiSearch...

Gene expression databases

BgeeiQ6T264.
CleanExiMM_MAML1.
ExpressionAtlasiQ6T264. baseline and differential.
GenevisibleiQ6T264. MM.

Family and domain databases

InterProiIPR019082. Neuroggenic_mastermind-like_N.
[Graphical view]
PfamiPF09596. MamL-1. 1 hit.
[Graphical view]
SMARTiSM01275. MamL-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional characterization of the murine mastermind-like 1 (Maml1) gene."
    Wu L., Kobayashi K., Sun T., Gao P., Liu J., Nakamura M., Weisberg E., Mukhopadhyay N.K., Griffin J.D.
    Gene 328:153-165(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NOTCH1; NOTCH2; NOTCH3 AND NOTCH4.
    Strain: BALB/cJImported.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryonic tailImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 130-1020.
    Strain: C57BL/6JImported and FVB/NImported.
    Tissue: BrainImported and Mammary glandImported.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 161-1020.
    Strain: C57BL/6J.
    Tissue: Head.
  6. "Mastermind critically regulates Notch-mediated lymphoid cell fate decisions."
    Maillard I., Weng A.P., Carpenter A.C., Rodriguez C.G., Sai H., Xu L., Allman D., Aster J.C., Pear W.S.
    Blood 104:1696-1702(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-127 AND SER-1019, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-310 AND SER-321, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung and Spleen.

Entry informationi

Entry nameiMAML1_MOUSE
AccessioniPrimary (citable) accession number: Q6T264
Secondary accession number(s): Q505D8
, Q5SUC2, Q6PDK3, Q6ZQG5, Q8BIU5, Q8R3T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: May 11, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.