Q6T1W8 (FDTA_ANETH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase
EC=5.3.2.3

Alternative name(s):
dTDP-6-deoxy-3,4-keto-hexulose isomerase

Gene names

Name:

fdtA

Organism

Aneurinibacillus thermoaerophilus

Taxonomic identifier

143495 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Aneurinibacillus group › Aneurinibacillus

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the isomerization of dTDP-6-deoxy-D-xylohex-4-ulose into dTDP-6-deoxy-D-xylohex-3-ulose in the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose, a glycan chain of the S-layer. Ref.1 Ref.2
Catalytic activity	dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose = dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose. Ref.1 Ref.2
Subunit structure	Homodimer. Ref.2

Ontologies

Keywords
Molecular function	Isomerase
Technical term	3D-structure
Gene Ontology (GO)
Molecular_function	isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 139

139

TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase

PRO_0000421823

Sites

Active site

49

1

Proton acceptor Probable

Experimental info

Mutagenesis

49

1

H → N: Loss of catalytic activity. Ref.2

Mutagenesis

51

1

H → N: Retains some catalytic activity. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

139

Helix Strand Turn

Details...

Beta strand

4 – 7

4

Beta strand

10 – 13

4

Beta strand

16 – 22

7

Turn

23 – 25

3

Beta strand

26 – 29

4

Beta strand

33 – 39

7

Beta strand

46 – 53

8

Beta strand

56 – 63

8

Beta strand

65 – 70

6

Beta strand

75 – 80

6

Beta strand

85 – 89

5

Beta strand

94 – 98

5

Beta strand

105 – 112

8

Helix

116 – 118

3

Helix

123 – 135

13

Sequences

	Sequence		Length	Mass (Da)
	Q6T1W8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EC32CB06F863F137	FASTA	139	16,042
	10 20 30 40 50 60 MENKVINFKK IIDSRGSLVA IEENKNIPFS IKRVYYIFDT KGEEPRGFHA HKKLEQVLVC 70 80 90 100 110 120 LNGSCRVILD DGNIIQEITL DSPAVGLYVG PAVWHEMHDF SSDCVMMVLA SDYYDETDYI 130 RQYDNFKKYI AKINLEKEG

References

[1]	"Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose in Aneurinibacillus thermoaerophilus L420-91T." Pfoestl A., Hofinger A., Kosma P., Messner P. J. Biol. Chem. 278:26410-26417(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY. Strain: L420-91T.
[2]	"The x-ray structure of dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase." Davis M.L., Thoden J.B., Holden H.M. J. Biol. Chem. 282:19227-19236(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS), SUBUNIT, ACTIVE SITE, FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF HIS-49 AND HIS-51.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY442352 Genomic DNA. Translation: AAS55720.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PA7	X-ray	1.50	A/B	1-139	[»]
2PAE	X-ray	2.50	A/B	1-139	[»]
2PAK	X-ray	2.40	A/B	1-139	[»]
2PAM	X-ray	2.50	A/B	1-139	[»]

ProteinModelPortal

Q6T1W8.

SMR

Q6T1W8. Positions 2-136.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-17003.

Family and domain databases

Gene3D

2.60.120.10. 1 hit.

InterPro

IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
IPR008894. WxcM_C.
[Graphical view]

Pfam

PF05523. FdtA. 1 hit.
[Graphical view]

SUPFAM

SSF51182. RmlC_like_cupin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q6T1W8.

Entry information

Entry name

FDTA_ANETH

Accession

Primary (citable) accession number: Q6T1W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2013
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program