Reviewed, UniProtKB/Swiss-Prot Q6SZS6 (PYRD2_KLUMA)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase, mitochondrial
      Short name=DHOdehase
      Short name=DHODase
      Short name=DHOD
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase

Gene names

Name:

URA9

Organism

Kluyveromyces marxianus (Yeast) (Candida kefyr)

Taxonomic identifier

4911 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol.
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Subcellular location	Mitochondrion inner membrane By similarity; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 446		Dihydroorotate dehydrogenase, mitochondrial		PRO_0000029894
Regions
	Transmembrane	42 – 58	17	Potential
Sites
	Active site	255	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6SZS6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EE025A136B2DA82B	FASTA	446	48,346
	10 20 30 40 50 60 MKYSFVSLLQ RGSIGSKQFI FFAKNEMSPL KAGVPGAKFL KYTVGFTLGT LAGFYLTNSR 70 80 90 100 110 120 SAIHEYVSCP TVRLLTPDAE AGHKLGIWLL KYGLAPRLWF DKDEELLHVN VFGKTLTNPV 130 140 150 160 170 180 GCAAGLDKDG EAIDGIFAGG FGYVEIGSVT PLPQPGNPKP RFFRLPMDEA VINRYGFNSS 190 200 210 220 230 240 GHDSVVKNLQ NRVSRFINSY FCNDINAVDN LSLYQNKLLG INLGKNKNGD EVQDYLKGVE 250 260 270 280 290 300 KFQKYADVLV INVSSPNTPG LRGLQKESIL TNLLSQVVAK RDSLVSSGNV LGAKTHRPPV 310 320 330 340 350 360 LVKVAPDLEE EEIKAIAEAA KKSKVDGIIV SNTTIQRPAS LITEDKNLVA QAGGLSGKPL 370 380 390 400 410 420 KPLSLKALKT MAHYTKGSGL VLVGCGGISS GADAIEFAKA GATMVELYTC YAYRGPGLIA 430 440 RVKDEITEQL KRENKTWSQI IGEDVK

References

[1]	"Contribution of horizontal gene transfer to the evolution of Saccharomyces cerevisiae." Hall C.R., Brachat S., Dietrich F.S. Eukaryot. Cell 4:1102-1115(2005) [PubMed: 15947202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 200963 / HA 63 / NRRL Y-8281.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY444339 Genomic DNA. Translation: AAR17522.1.
3D structure databases
SMR	Q6SZS6. Positions 78-446.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.3.1. 97088.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD2_KLUMA

Accession

Primary (citable) accession number: Q6SZS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)