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Q6SW84

- DEN_HCMVM

UniProt

Q6SW84 - DEN_HCMVM

Protein

Deneddylase

Gene

UL48

Organism
Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei24 – 241PROSITE-ProRule annotation
    Active sitei160 – 1601PROSITE-ProRule annotation
    Active sitei162 – 1621PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Large tegument protein
    Short name:
    LTP
    Gene namesi
    Name:UL48
    OrganismiHuman cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
    Taxonomic identifieri295027 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000000938: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22412241DeneddylasePRO_0000416709Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6SW84.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 226223Peptidase C76PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili544 – 57128Sequence AnalysisAdd
    BLAST
    Coiled coili765 – 79228Sequence AnalysisAdd
    BLAST
    Coiled coili1167 – 120034Sequence AnalysisAdd
    BLAST
    Coiled coili1240 – 127233Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi244 – 2474Poly-Thr
    Compositional biasi325 – 3284Poly-Ser
    Compositional biasi785 – 7895Poly-Glu
    Compositional biasi1921 – 193010Poly-Thr
    Compositional biasi2133 – 21375Poly-Ala

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6SW84-1 [UniParc]FASTAAdd to Basket

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    MKVTQASCHQ GDIARFGARA GNQCVCNGIM FLHALHLGGT SAVLQTEALD     50
    AIMEEGARLD ARLERELQKK LPAGGRLPVY RLGDEVPRRL ESRFGRTVHA 100
    LSRPFNGTTE TCDLDGYMCP GIFDFLRYAH AKPRPTYVLV TVNSLARAVV 150
    FTEDHMLVFD PHSSAECHNA AVYHCEGLHQ VLMVLTGFGV QLSPAFYYEA 200
    LFLYMLDVAT VPEAEIAARL VSTYRDRDID LTGVVRESAD TAATTTTAAP 250
    SLPPLPDPIV DPGCPPGVAP SIPVYDPSSS PKKTPEKRRK DLSGSKHGGK 300
    KKPPSTTSKT LATASSSPSA IAAASSSSAV PPSYSCGEGA LPALGRYQQL 350
    VDEVEQELKA LTLPPLPANT SAWTLHAAGT ESGANAATAT APSFDEAFLT 400
    DRLQQLIIHA VNQRSCLRRP CGPQSAAQQA VRAYLGLSKK LDAFLLNWLH 450
    HGLDLQRMHD YLSHKTTKGT YSTLDRALLE KMQVVFDPYG RQHGPALIAW 500
    VEEMLRYVES KPTNELSQRL QRFVTKRPMP VSDSFVCLRP VDFQRLTQVI 550
    EQRRRVLQRQ REEYHGVYEH LAGLITSIDI HDLDASDLNR REILKALQPL 600
    DDNAKQELFR LGNAKMLELQ MDLDRLSTQL LTRVHNHILN GFLPVEDLKQ 650
    MERVVEQVLR LFYDLRDLKL CDGSYEEGFV VIREQLSYLM TGTVRDNVPL 700
    LQEILQLRHA YQQATQQNEG RLTQIHDLLH VIETLVRDPG SRGSALTLAL 750
    VQEQLAQLEA LGGLQLPEVQ QRLQNAQLAL SRLYEEEEET QRFLDGLSYD 800
    DPPTEQTIKR HPQLREMLRR DEQTRLRLIN AVLSMFHTLV MRLARDESPR 850
    PTFFDAVSLL LQQLPPDSHE REDLRAANAT YAQMVKKLEQ IEKAGTGASE 900
    KRFQALRELV YFFRNHEYFF QHMVGRLGVG PQVTELYERY QHEMEEQHLE 950
    RLEREWQEEA GKLTVTSVED VQRVLARAPS HRVMHQMQQT LTTKMQDFLD 1000
    KEKRKQEEQQ RQLLDGYQKK VQQDLQRVVD AVKGEMLSTI PHQPLEATLE 1050
    LLLGLDQRAQ PLLDKFNQDL LSALQQLSKK LDGRINECLH GVLTGDVERR 1100
    CHPHREAAMQ TQASLNHLDQ ILGPQLLIHE TQQALQHAVH QAQFIEKCQQ 1150
    GDPTTAITGS EFESDFARYR SSQQKMEGQL QETRQQMTET SERLDRSLRQ 1200
    DPGSSSVTRV PEKPFKGQEL AGRITPPPAD FQRPVFKTLL DQQADAARKA 1250
    LSDEADLLNQ KVQTQLRQRD EQLSTAQNLW TDLVTRHKMS GGLDVTTPDA 1300
    KALMEKPLET LRELLGKATQ QLPYLSAERT VRWMLAFLEE ALAQITADPT 1350
    HPHHGSRTHY RNLQQQAVES AVTLAHQIEQ NAACENFIAQ HQEATANGAS 1400
    TPRVDMVQAV EAVWQRLEPG RVAGGAARHQ KVQELLQRLG QTLGDLELQE 1450
    TLATEYFALL HGIQTFSYGL DFRSQLEKIR DLRTRFAELA KRCGTRLSNE 1500
    GALPNPRKPQ ATTSLGAFTR GLNALERHVQ LGHQYLLNKL NGSSLVYRLE 1550
    DIPSVLPPTH ETDPALIMRD RLRRLCFARH HDTFLEVVDV FGMRQIVTQA 1600
    GEPIYLVTDY GNVAFKYLAL RDDGRPLAWR RRCSGGGLKN VVTTRYKAIT 1650
    VAVAVCQTLR TFWPQISQYD LRPYLTQHQS HTHPAETHTL HNLKLFCYLV 1700
    STAWHQRIDT QQELTAADRV GSGEGGDVGE QRPGRGTVLR LSLQEFCVLI 1750
    AALYPEYIYT VLKYPVQMSL PSLTAHLHQD VIHAVVNNTH KMPPDHLPEQ 1800
    VKAFCITPTQ WPAMQLNKLF WENKLVQQLC QVGPQKSTPS LGKLWLYAMA 1850
    TLVFPQDMLQ CLWLELKPQY AETYASVSEL VQTLFQIFTQ QCEMVTEGYT 1900
    QPQLPTGEPV LQMIRVRRQD TTTTDTNTTT EPGLLDVFIQ TETALDYALG 1950
    SWLFGIPVCL GVHVADLLKG QRVLVARHLE YTSRDRDFLR IQRSRDLNLS 2000
    QLLQDTWTET PLEHCWLQAQ IRRLRDYLRF PTRLEFIPLV IYNAQDHTVV 2050
    RVLRPPSTFE QDHSRLVLDE AFPTFPLYDQ DDNTSADNVA ASGAAPTPPV 2100
    PFNRVPVNIQ FLRENPPPIA RVQQPPRRHR HRAAAAADDD GQIDHAQDDT 2150
    SRTADSALVS TAFGGSVFQE NRLGETPLCR DELVAVAPGA ASTSFASPPI 2200
    TVLTQNVLSA LEILRLVRLD LRQLAQSVQD TIQHMRFLYL L 2241
    Length:2,241
    Mass (Da):253,174
    Last modified:July 5, 2004 - v1
    Checksum:i83C76E6D72FA039B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY446894 Genomic DNA. Translation: AAR31613.1.
    RefSeqiYP_081506.1. NC_006273.2.

    Genome annotation databases

    GeneIDi3077504.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY446894 Genomic DNA. Translation: AAR31613.1 .
    RefSeqi YP_081506.1. NC_006273.2.

    3D structure databases

    ProteinModelPortali Q6SW84.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3077504.

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiDEN_HCMVM
    AccessioniPrimary (citable) accession number: Q6SW84
    Secondary accession number(s): D2K3L5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3