Q6SW84 (DEN_HCMVM) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 9, 2013.
Version 39.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Deneddylase UL48 EC=3.4.19.12 EC=3.4.22.- Alternative name(s): Large tegument protein Short name=LTP | ||
| Gene names |
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| Organism | Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5) [Reference proteome] | ||
| Taxonomic identifier | 295027 [NCBI] | ||
| Taxonomic lineage | Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Betaherpesvirinae › Cytomegalovirus ›  | ||
| Virus host | Homo sapiens (Human) [TaxID: 9606] |
Protein attributes
| Sequence length | 2241 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity. |
| Subcellular location | Virion tegument. Host cytoplasm. Host nucleus By similarity. |
| Sequence similarities | Belongs to the herpesviridae large tegument protein family. Contains 1 peptidase C76 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Host-virus interaction Modulation of host ubiquitin pathway by viral deubiquitinase Modulation of host ubiquitin pathway by virus Ubl conjugation pathway |
| Cellular component | Host cytoplasm Host nucleus Virion Virion tegument |
| Domain | Coiled coil Repeat |
| Molecular function | Hydrolase Protease Thiol protease |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | modulation by virus of host protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleusInferred from electronic annotation. Source: UniProtKB-SubCell viral tegumentInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 2241 | 2241 | Deneddylase UL48 | PRO_0000416709 | |||||
Regions | |||||||||
| Domain | 4 – 226 | 223 | Peptidase C76 | ||||||
| Coiled coil | 544 – 571 | 28 | Potential | ||||||
| Coiled coil | 765 – 792 | 28 | Potential | ||||||
| Coiled coil | 1167 – 1200 | 34 | Potential | ||||||
| Coiled coil | 1240 – 1272 | 33 | Potential | ||||||
| Compositional bias | 244 – 247 | 4 | Poly-Thr | ||||||
| Compositional bias | 325 – 328 | 4 | Poly-Ser | ||||||
| Compositional bias | 785 – 789 | 5 | Poly-Glu | ||||||
| Compositional bias | 1921 – 1930 | 10 | Poly-Thr | ||||||
| Compositional bias | 2133 – 2137 | 5 | Poly-Ala | ||||||
Sites | |||||||||
| Active site | 24 | 1 | By similarity | ||||||
| Active site | 160 | 1 | By similarity | ||||||
| Active site | 162 | 1 | By similarity | ||||||
Sequences
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References
| [1] | "Genetic content of wild-type human cytomegalovirus." Dolan A., Cunningham C., Hector R.D., Hassan-Walker A.F., Lee L., Addison C., Dargan D.J., McGeoch D.J., Gatherer D., Emery V.C., Griffiths P.D., Sinzger C., McSharry B.P., Wilkinson G.W.G., Davison A.J. J. Gen. Virol. 85:1301-1312(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY446894 Genomic DNA. Translation: AAR31613.1. |
| RefSeq | YP_081506.1. NC_006273.2. |
3D structure databases | |
| ProteinModelPortal | Q6SW84. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 3077504. |
Phylogenomic databases | |
| ProtClustDB | CLSP2510059. |
Family and domain databases | |
| InterPro | IPR006928. Pept_C76_UL36-USP. [Graphical view] |
| Pfam | PF04843. Herpes_teg_N. 1 hit. [Graphical view] |
| PROSITE | PS51521. HTUSP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DEN_HCMVM | ||||||||
| Accession | Primary (citable) accession number: Q6SW84 Secondary accession number(s): D2K3L5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Viral Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |