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Protein

Nuclear egress protein 2

Gene

NEC2

Organism
Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in virion nuclear egress, the first step of virion release from infected cell. Within the host nucleus, NEC1 interacts with the newly formed capsid through the vertexes and directs it to the inner nuclear membrane by associating with NEC2. Induces the budding of the capsid at the inner nuclear membrane as well as its envelopment into the perinuclear space. There, the NEC1/NEC2 complex promotes the fusion of the enveloped capsid with the outer nuclear membrane and the subsequent release of the viral capsid into the cytoplasm where it will reach the secondary budding sites in the host Golgi or trans-Golgi network.UniRule annotation

GO - Biological processi

  • viral budding from nuclear membrane Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear egress protein 2UniRule annotation
Gene namesi
Name:NEC2UniRule annotation
Ordered Locus Names:UL50
OrganismiHuman cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
Taxonomic identifieri295027 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000000938 Componenti: Genome

Subcellular locationi

  • Host nucleus inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation
  • Note: Localizes also at the transient membrane of perinuclear virions.UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 359Perinuclear spaceUniRule annotationAdd BLAST359
Transmembranei360 – 382HelicalUniRule annotationAdd BLAST23
Topological domaini383 – 398NuclearUniRule annotationAdd BLAST16

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Host nucleus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004167191 – 398Nuclear egress protein 2Add BLAST398

Post-translational modificationi

Phosphorylated.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ6SW81

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Forms a heterohexameric complex with NEC1.UniRule annotation

Structurei

Secondary structure

1398
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 19Combined sources15
Helixi23 – 25Combined sources3
Beta strandi26 – 28Combined sources3
Helixi31 – 36Combined sources6
Beta strandi42 – 47Combined sources6
Helixi55 – 66Combined sources12
Beta strandi71 – 76Combined sources6
Beta strandi81 – 88Combined sources8
Beta strandi102 – 106Combined sources5
Beta strandi111 – 114Combined sources4
Helixi116 – 122Combined sources7
Beta strandi126 – 128Combined sources3
Beta strandi130 – 137Combined sources8
Beta strandi139 – 142Combined sources4
Beta strandi144 – 152Combined sources9
Helixi156 – 169Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5D5NX-ray2.44A1-175[»]
SMRiQ6SW81
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi217 – 222Poly-Pro6
Compositional biasi241 – 247Poly-Pro7
Compositional biasi256 – 267Cys-richAdd BLAST12
Compositional biasi275 – 279Poly-Glu5
Compositional biasi289 – 294Poly-Ala6

Sequence similaritiesi

Belongs to the herpesviridae NEC2 protein family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090000J9

Family and domain databases

HAMAPiMF_04024 HSV_NEC2, 1 hit
InterProiView protein in InterPro
IPR007626 Herpesvirus_viron_egress-type
PfamiView protein in Pfam
PF04541 Herpes_U34, 1 hit

Sequencei

Sequence statusi: Complete.

Q6SW81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMNKVLHQD LVQATRRILK LGPSELRVTD AGLICKNPNY SVCDAMLKTD
60 70 80 90 100
TVYCVEYLLS YWESRTDHVP CFIFKNTGCA VSLCCFVRAP VKLVSPARHV
110 120 130 140 150
GEFNVLKVNE SLIVTLKDIE EIKPSAYGVL TKCVVRKSNS ASVFNIELIA
160 170 180 190 200
FGPENEGEYE NLLRELYAKK AASTSLAVRN HVTVSSHSGS GPSLWRARMS
210 220 230 240 250
AALTRTAGKR SPRTASPPPP PPRHPSCSPT MVAAGGAAAG PRPPPPPMAA
260 270 280 290 300
GSWRLCRCEA CMGRCGCASE GDADEEEEEL LALAGEGKAA AAAAGQDIGG
310 320 330 340 350
SARRPLEEHV SRRRGVSTHH RHPPSPPCTP SLERTGYRWA PSSWWRARSG
360 370 380 390
PSRPQSGPWL PARFATLGPL VLALLLVLAL LWRGHGQSSS PTRSAHRD
Length:398
Mass (Da):43,052
Last modified:July 5, 2004 - v1
Checksum:i367FF459968AD468
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY446894 Genomic DNA Translation: AAR31616.1
RefSeqiYP_081509.1, NC_006273.2

Genome annotation databases

GeneIDi3077439
KEGGivg:3077439

Similar proteinsi

Entry informationi

Entry nameiNEC2_HCMVM
AccessioniPrimary (citable) accession number: Q6SW81
Secondary accession number(s): D2K3L8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 47 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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