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Protein

Viral transcription factor IE2

Gene

UL122

Organism
Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Stimulates viral early and late gene expression and thus play a crucial role in the regulation of productive infection. In addition, activates quiescent cells to reenter the cell cycle and upregulates several E2F-responsive genes, which are responsible for pushing the cell into S phase. In S-phase, inhibits cellular DNA synthesis and blocks further cell cycle progression (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

G0/G1 host cell cycle checkpoint dysregulation by virus, G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Viral transcription factor IE2
Short name:
IE2
Alternative name(s):
Protein UL122
Gene namesi
Name:UL122
OrganismiHuman cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
Taxonomic identifieri295027 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000000938 Componenti: Genome

Subcellular locationi

  • Host nucleus

  • Note: Colocalizes with host PML-associated nuclear bodies.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Viral transcription factor IE2PRO_0000417856Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki175 – 175Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki180 – 180Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Sumoylated. The sumoylation is necessary for efficient replication of the virus and thus for the function of this viral transcription factor (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Interacts with host SUMO-modified form of TATA-binding protein (TBP)-associated factor 12/TAF12 in a SIM-dependent manner; this interaction increases the transactivation activity of IE2. Interacts with host CHAF1A. Interacts with several components of the host transcriptional machinery including TBP, TF2B and CREB1. Interacts with host DNA replication licensing factor MCM3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi1678116. 9 interactions.
IntActiQ6SW29. 8 interactions.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni200 – 2089Non-covalent SUMO1 binding region (SIM)By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi258 – 32063Gly/Ser-richAdd
BLAST
Compositional biasi258 – 27619Poly-SerAdd
BLAST

Domaini

The SUMO-interacting motif (SIM) is required for efficient transactivation function.By similarity

Sequence similaritiesi

Belongs to the HHV-5 IE2 protein family.Curated

Family and domain databases

InterProiIPR010855. Cytomega_IE1.
IPR005028. Herpes_IE2_3.
[Graphical view]
PfamiPF07340. Herpes_IE1. 1 hit.
PF03361. Herpes_IE2_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6SW29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSAKRKMD PDNPDEGPSS KVPRPETPVT KATTFLQTML RKEVNSQLSL
60 70 80 90 100
GDPLFPELAE ESLKTFEQVT EDCNENPEKD VLTELGDILA QAVNHAGIDS
110 120 130 140 150
SSTGPTLTTH SCSVSSAPLN KPTPTSVAVT NTPLPGASAT PELSPRKKPR
160 170 180 190 200
KTTRPFKVII KPPVPPAPIM LPLIKQEDIK PEPDFTIQYR NKIIDTAGCI
210 220 230 240 250
VISDSEEEQG EEVETRGATA SSPSTGSGTP RVTSPTHPLS QMNHPPLPDP
260 270 280 290 300
LGRPDEDSSS SSSSSCSSAS DSESESEEMK CSSGGGASVT SSHHGRGGFG
310 320 330 340 350
GAASSSLLSC GHQSSGGAST GPRKKKSKRI SELDNEKVRN IMKDKNTPFC
360 370 380 390 400
TPNVQTRRGR VKIDEVSRMF RHTNRSLEYK NLPFMIPSMH QVLEEAIKVC
410 420 430 440 450
KTMQVNNKGI QIIYTRNHEV KNEVDQVRCR LGSMCNLALS TPFLMEHTMP
460 470 480 490 500
VTHPPDVAQR TADACNDGVK AVWNLKELHT HQLCPRSSDY RNMIIHAATP
510 520 530 540 550
VDLLGALNLC LPLMQKFPKQ VMVRIFSTNQ GGFMLPIYET AAKAYAVGQF
560 570 580
EKPTETPPED LDTLSLAIEA AIQDLRNKSQ
Length:580
Mass (Da):63,097
Last modified:July 5, 2004 - v1
Checksum:i215BAFA207681B77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY446894 Genomic DNA. Translation: AAR31665.1.
RefSeqiYP_081561.1. NC_006273.2.

Genome annotation databases

GeneIDi3077563.
KEGGivg:3077563.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY446894 Genomic DNA. Translation: AAR31665.1.
RefSeqiYP_081561.1. NC_006273.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1678116. 9 interactions.
IntActiQ6SW29. 8 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3077563.
KEGGivg:3077563.

Family and domain databases

InterProiIPR010855. Cytomega_IE1.
IPR005028. Herpes_IE2_3.
[Graphical view]
PfamiPF07340. Herpes_IE1. 1 hit.
PF03361. Herpes_IE2_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiVIE2_HCMVM
AccessioniPrimary (citable) accession number: Q6SW29
Secondary accession number(s): D2K3S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: July 5, 2004
Last modified: November 11, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.