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Q6STF1

- OXLA_GLOHA

UniProt

Q6STF1 - OXLA_GLOHA

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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Gloydius halys (Chinese water mocassin) (Agkistrodon halys)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, antibacterial and antiparasitic activities, as well as regulation of platelet aggregation. Its effect on platelets is controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions (By similarity). This protein induces apoptosis of cultured HeLa cells.By similarity1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FAD.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891FAD2 Publications
Binding sitei108 – 1081Substrate
Binding sitei241 – 2411Substrate
Binding sitei279 – 2791FAD; via amide nitrogen and carbonyl oxygen2 Publications
Binding sitei390 – 3901Substrate
Binding sitei475 – 4751FAD2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 622FAD2 Publications
Nucleotide bindingi81 – 822FAD2 Publications
Nucleotide bindingi105 – 1084FAD2 Publications
Nucleotide bindingi482 – 4876FAD2 Publications
Nucleotide bindingi482 – 4832Substrate

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. hemolysis in other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Hemorrhagic toxin, Hemostasis impairing toxin, Oxidoreductase, Toxin

Keywords - Biological processi

Apoptosis, Cytolysis, Hemolysis

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
AHP-LAAO
Short name:
LAAO
Short name:
LAO
OrganismiGloydius halys (Chinese water mocassin) (Agkistrodon halys)
Taxonomic identifieri8714 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeGloydius

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Chaini19 – 504486L-amino-acid oxidasePRO_0000273563Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 191
Glycosylationi190 – 1901N-linked (GlcNAc...)2 Publications
Disulfide bondi349 ↔ 430
Glycosylationi379 – 3791N-linked (GlcNAc...)2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer; non-covalently linked.2 Publications

Structurei

Secondary structure

1
504
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 294
Helixi34 – 4310
Beta strandi53 – 575
Helixi61 – 7212
Beta strandi76 – 805
Beta strandi82 – 865
Beta strandi92 – 954
Turni96 – 994
Beta strandi100 – 1056
Beta strandi109 – 1124
Helixi114 – 1229
Beta strandi127 – 1293
Beta strandi137 – 1415
Beta strandi144 – 1474
Helixi148 – 1536
Helixi155 – 1584
Helixi164 – 1663
Helixi171 – 1788
Helixi180 – 1889
Helixi191 – 1977
Helixi198 – 2003
Helixi203 – 2097
Helixi215 – 22410
Helixi228 – 2303
Beta strandi231 – 2344
Helixi235 – 24511
Beta strandi251 – 2544
Helixi260 – 2689
Helixi269 – 2724
Beta strandi273 – 2764
Beta strandi278 – 2858
Beta strandi288 – 2947
Beta strandi296 – 2983
Beta strandi300 – 31011
Helixi314 – 3185
Beta strandi320 – 3245
Helixi328 – 3369
Beta strandi342 – 35110
Helixi353 – 3575
Beta strandi363 – 3686
Beta strandi372 – 3743
Beta strandi385 – 3928
Helixi393 – 3975
Turni398 – 4014
Helixi404 – 41916
Helixi423 – 4297
Beta strandi430 – 4378
Helixi438 – 4403
Turni442 – 4443
Beta strandi446 – 4494
Helixi455 – 46410
Beta strandi470 – 4723
Helixi475 – 4773
Beta strandi478 – 4803
Helixi484 – 50320

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1REOX-ray2.31A19-504[»]
1TDKX-ray2.70A19-504[»]
1TDNX-ray2.70A19-504[»]
1TDOX-ray3.00A19-504[»]
ProteinModelPortaliQ6STF1.
SMRiQ6STF1. Positions 21-504.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6STF1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG005729.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6STF1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNVFFMFSLL FLAALGSCAN DRNPLEECFR ETDYEEFLEI ARNGLKATSN
60 70 80 90 100
PKHVVVVGAG MSGLSAAYVL SGAGHQVTVL EASERAGGRV RTYRNDKEDW
110 120 130 140 150
YANLGPMRLP EKHRIVREYI RKFGLQLNEF SQENDNAWYF IKNIRKRVGE
160 170 180 190 200
VKKDPGVLKY PVKPSEEGKS AGQLYEESLG KVVEELKRTN CSYILNKYDT
210 220 230 240 250
YSTKEYLLKE GNLSPGAVDM IGDLMNEDSG YYVSFPESLR HDDIFAYEKR
260 270 280 290 300
FDEIVGGMDK LPTSMYRAIE EKVHLNAQVI KIQKNAEKVT VVYQTPAKEM
310 320 330 340 350
ASVTADYVIV CTTSRATRRI KFEPPLPPKK AHALRSVHYR SGTKIFLTCT
360 370 380 390 400
KKFWEDEGIH GGKSTTDLPS RFIYYPNHNF TSGVGVIIAY GIGDDANFFQ
410 420 430 440 450
ALDFKDCADI VINDLSLIHQ LPREEIQTFC YPSMIQKWSL DKYAMGGITT
460 470 480 490 500
FTPYQFQHFS ESLTASVDRI YFAGEHTAEA HGWIDSTIKS GLRAARDVNR

ASEQ
Length:504
Mass (Da):57,125
Last modified:July 5, 2004 - v1
Checksum:iAEB034155184F0A7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY450403 mRNA. Translation: AAR20248.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY450403 mRNA. Translation: AAR20248.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1REO X-ray 2.31 A 19-504 [» ]
1TDK X-ray 2.70 A 19-504 [» ]
1TDN X-ray 2.70 A 19-504 [» ]
1TDO X-ray 3.00 A 19-504 [» ]
ProteinModelPortali Q6STF1.
SMRi Q6STF1. Positions 21-504.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG005729.

Miscellaneous databases

EvolutionaryTracei Q6STF1.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Zhang H., Zhang T., Teng M., Niu L.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  2. "Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom."
    Zhang H., Teng M., Niu L., Wang Y., Wang Y., Liu Q., Huang Q., Hao Q., Dong Y., Liu P.
    Acta Crystallogr. D 60:974-977(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 19-504 IN COMPLEX WITH FAD, PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS, GLYCOSYLATION AT ASN-190 AND ASN-379, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Venom.
  3. "Structures of L-amino acid oxidase in complex with substrates and substrate analogue."
    Zhang H., Teng M., Niu L.
    Submitted (MAY-2004) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 19-504 IN COMPLEX WITH FAD AND SUBSTRATE ANALOGS, DISULFIDE BONDS, GLYCOSYLATION AT ASN-190 AND ASN-379.

Entry informationi

Entry nameiOXLA_GLOHA
AccessioniPrimary (citable) accession number: Q6STF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3