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Q6STF1

- OXLA_GLOHA

UniProt

Q6STF1 - OXLA_GLOHA

Protein

L-amino-acid oxidase

Gene
N/A
Organism
Gloydius halys (Chinese water mocassin) (Agkistrodon halys)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, antibacterial and antiparasitic activities, as well as regulation of platelet aggregation. Its effect on platelets is controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions By similarity. This protein induces apoptosis of cultured HeLa cells.By similarity1 Publication

    Catalytic activityi

    An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

    Cofactori

    FAD.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei89 – 891FAD2 Publications
    Binding sitei108 – 1081Substrate
    Binding sitei241 – 2411Substrate
    Binding sitei279 – 2791FAD; via amide nitrogen and carbonyl oxygen2 Publications
    Binding sitei390 – 3901Substrate
    Binding sitei475 – 4751FAD2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi61 – 622FAD2 Publications
    Nucleotide bindingi81 – 822FAD2 Publications
    Nucleotide bindingi105 – 1084FAD2 Publications
    Nucleotide bindingi482 – 4876FAD2 Publications
    Nucleotide bindingi482 – 4832Substrate

    GO - Molecular functioni

    1. L-amino-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. defense response to bacterium Source: UniProtKB-KW
    3. hemolysis in other organism Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Hemorrhagic toxin, Hemostasis impairing toxin, Oxidoreductase, Toxin

    Keywords - Biological processi

    Apoptosis, Cytolysis, Hemolysis

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-amino-acid oxidase (EC:1.4.3.2)
    Short name:
    AHP-LAAO
    Short name:
    LAAO
    Short name:
    LAO
    OrganismiGloydius halys (Chinese water mocassin) (Agkistrodon halys)
    Taxonomic identifieri8714 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeGloydius

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Chaini19 – 504486L-amino-acid oxidasePRO_0000273563Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 191
    Glycosylationi190 – 1901N-linked (GlcNAc...)2 Publications
    Disulfide bondi349 ↔ 430
    Glycosylationi379 – 3791N-linked (GlcNAc...)2 Publications

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Interactioni

    Subunit structurei

    Homodimer; non-covalently linked.2 Publications

    Structurei

    Secondary structure

    1
    504
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi26 – 294
    Helixi34 – 4310
    Beta strandi53 – 575
    Helixi61 – 7212
    Beta strandi76 – 805
    Beta strandi82 – 865
    Beta strandi92 – 954
    Turni96 – 994
    Beta strandi100 – 1056
    Beta strandi109 – 1124
    Helixi114 – 1229
    Beta strandi127 – 1293
    Beta strandi137 – 1415
    Beta strandi144 – 1474
    Helixi148 – 1536
    Helixi155 – 1584
    Helixi164 – 1663
    Helixi171 – 1788
    Helixi180 – 1889
    Helixi191 – 1977
    Helixi198 – 2003
    Helixi203 – 2097
    Helixi215 – 22410
    Helixi228 – 2303
    Beta strandi231 – 2344
    Helixi235 – 24511
    Beta strandi251 – 2544
    Helixi260 – 2689
    Helixi269 – 2724
    Beta strandi273 – 2764
    Beta strandi278 – 2858
    Beta strandi288 – 2947
    Beta strandi296 – 2983
    Beta strandi300 – 31011
    Helixi314 – 3185
    Beta strandi320 – 3245
    Helixi328 – 3369
    Beta strandi342 – 35110
    Helixi353 – 3575
    Beta strandi363 – 3686
    Beta strandi372 – 3743
    Beta strandi385 – 3928
    Helixi393 – 3975
    Turni398 – 4014
    Helixi404 – 41916
    Helixi423 – 4297
    Beta strandi430 – 4378
    Helixi438 – 4403
    Turni442 – 4443
    Beta strandi446 – 4494
    Helixi455 – 46410
    Beta strandi470 – 4723
    Helixi475 – 4773
    Beta strandi478 – 4803
    Helixi484 – 50320

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1REOX-ray2.31A19-504[»]
    1TDKX-ray2.70A19-504[»]
    1TDNX-ray2.70A19-504[»]
    1TDOX-ray3.00A19-504[»]
    ProteinModelPortaliQ6STF1.
    SMRiQ6STF1. Positions 21-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6STF1.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG005729.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6STF1-1 [UniParc]FASTAAdd to Basket

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    MNVFFMFSLL FLAALGSCAN DRNPLEECFR ETDYEEFLEI ARNGLKATSN    50
    PKHVVVVGAG MSGLSAAYVL SGAGHQVTVL EASERAGGRV RTYRNDKEDW 100
    YANLGPMRLP EKHRIVREYI RKFGLQLNEF SQENDNAWYF IKNIRKRVGE 150
    VKKDPGVLKY PVKPSEEGKS AGQLYEESLG KVVEELKRTN CSYILNKYDT 200
    YSTKEYLLKE GNLSPGAVDM IGDLMNEDSG YYVSFPESLR HDDIFAYEKR 250
    FDEIVGGMDK LPTSMYRAIE EKVHLNAQVI KIQKNAEKVT VVYQTPAKEM 300
    ASVTADYVIV CTTSRATRRI KFEPPLPPKK AHALRSVHYR SGTKIFLTCT 350
    KKFWEDEGIH GGKSTTDLPS RFIYYPNHNF TSGVGVIIAY GIGDDANFFQ 400
    ALDFKDCADI VINDLSLIHQ LPREEIQTFC YPSMIQKWSL DKYAMGGITT 450
    FTPYQFQHFS ESLTASVDRI YFAGEHTAEA HGWIDSTIKS GLRAARDVNR 500
    ASEQ 504
    Length:504
    Mass (Da):57,125
    Last modified:July 5, 2004 - v1
    Checksum:iAEB034155184F0A7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY450403 mRNA. Translation: AAR20248.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY450403 mRNA. Translation: AAR20248.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1REO X-ray 2.31 A 19-504 [» ]
    1TDK X-ray 2.70 A 19-504 [» ]
    1TDN X-ray 2.70 A 19-504 [» ]
    1TDO X-ray 3.00 A 19-504 [» ]
    ProteinModelPortali Q6STF1.
    SMRi Q6STF1. Positions 21-504.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG005729.

    Miscellaneous databases

    EvolutionaryTracei Q6STF1.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Zhang H., Zhang T., Teng M., Niu L.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Venom gland.
    2. "Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom."
      Zhang H., Teng M., Niu L., Wang Y., Wang Y., Liu Q., Huang Q., Hao Q., Dong Y., Liu P.
      Acta Crystallogr. D 60:974-977(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 19-504 IN COMPLEX WITH FAD, PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS, GLYCOSYLATION AT ASN-190 AND ASN-379, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Venom.
    3. "Structures of L-amino acid oxidase in complex with substrates and substrate analogue."
      Zhang H., Teng M., Niu L.
      Submitted (MAY-2004) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 19-504 IN COMPLEX WITH FAD AND SUBSTRATE ANALOGS, DISULFIDE BONDS, GLYCOSYLATION AT ASN-190 AND ASN-379.

    Entry informationi

    Entry nameiOXLA_GLOHA
    AccessioniPrimary (citable) accession number: Q6STF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3