Q6SKG1 (ACSM3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-coenzyme A synthetase ACSM3, mitochondrial
EC=6.2.1.2

Alternative name(s):
Acyl-CoA synthetase medium-chain family member 3
Butyrate--CoA ligase 3
Butyryl-coenzyme A synthetase 3
Middle-chain acyl-CoA synthetase 3
SA rat hypertension-associated protein
Short name=Protein SA

Gene names

Name:	Acsm3
Synonyms:	Sah

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro) By similarity.
Catalytic activity	ATP + an acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW butyrate-CoA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 21	21	Mitochondrion Potential
	Chain	22 – 580	559	Acyl-coenzyme A synthetase ACSM3, mitochondrial		PRO_0000306100
Regions
	Nucleotide binding	229 – 237	9	ATP By similarity
	Nucleotide binding	368 – 373	6	ATP By similarity
Sites
	Binding site	455	1	ATP By similarity
	Binding site	470	1	ATP By similarity
	Binding site	566	1	ATP By similarity
Amino acid modifications
	Modified residue	37	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6SKG1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E2F46C5C530D6278	FASTA	580	65,713
	10 20 30 40 50 60 MAMLLRARCF HRLAIPDPRR ILYKDYRTAI PQNFSNYESM KHDFKIEIPE YFNFAKDVLD 70 80 90 100 110 120 QWTNTEKTGK RLSNPAFWWV DGNGKEVRWS FEELGSLSRK FANILTEACS LQRGDRVMVI 130 140 150 160 170 180 LPKIPEWWLA NVACLRTGTV LIPGTTQLTQ KDILYRLQSS KSKCIITDDT LAPAVDIVAA 190 200 210 220 230 240 KCENLHSKLI VSQHSREGWG NLKEMMKYAS DSHTCVDTKH NELMAIYFTS GTTGPPKMIG 250 260 270 280 290 300 HTHSSFGLGL SVNGRFWLDL IASDVMWNTS DTGWAKSAWS SVFSPWTQGA CVFAHYLPRF 310 320 330 340 350 360 DSTSILQTLS KFPITVFCSA PTAYRMLIQN DITSYKFNSL KHCVSAGEPI NPEVMEQWKK 370 380 390 400 410 420 KTGLDIYEGY GQTETVLICG NFKGMKIKPG SMGKPSPAFN VEILDENGTI LPPGQEGDIA 430 440 450 460 470 480 VQVLPDRPFG LFTHYVDNPS KTASTLRGNF YITGDRGYMD EDGYFWFVAR SDDVILSSGY 490 500 510 520 530 540 RIGPFEVESA LIEHPSIAES AVVSSPDPIR GEVVKAFIVL NPDYKLHDQE QLKKEIQEHV 550 560 570 580 KKTTAPYKYP RKIEFIEELP KTVSGKVKRN ELRRKEWTTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Exon repetition: a major pathway for processing mRNA of some genes is allele-specific." Rigatti R., Jia J.-H., Samani N.J., Eperon I.C. Nucleic Acids Res. 32:441-446(2004) [PubMed: 14739236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SHR and Wistar Kyoto.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Possible alternative splicing of the rat SA gene." Gu L., Dene H., Rapp J.P. Mamm. Genome 6:683-684(1995) [PubMed: 8535086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 374-479.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY455861 Genomic DNA. Translation: AAR20710.1. AY456695 Genomic DNA. Translation: AAR21570.1. BC090325 mRNA. Translation: AAH90325.1. U19832 Genomic DNA. Translation: AAA95995.1.
IPI	IPI00421616.
RefSeq	NP_150234.1. NM_033231.1.
UniGene	Rn.88644.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ProteinModelPortal	Q6SKG1.
SMR	Q6SKG1. Positions 46-578.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6SKG1.
Proteomic databases
PRIDE	Q6SKG1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24763.
KEGG	rno:24763.
Organism-specific databases
CTD	6296.
RGD	62086. Acsm3.
Phylogenomic databases
eggNOG	maNOG11567.
GeneTree	ENSGT00550000074278.
HOVERGEN	HBG053031.
InParanoid	Q6SKG1.
Gene expression databases
ArrayExpress	Q6SKG1.
Genevestigator	Q6SKG1.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01896.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604315.

Entry information

Entry name

ACSM3_RAT

Accession

Primary (citable) accession number: Q6SKG1
Secondary accession number(s): Q62742

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program