Reviewed, UniProtKB/Swiss-Prot Q6SJV8 (CRD1_GOSHI)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	CRD1
Synonyms:	ZIP

Organism

Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)

Taxonomic identifier

3635 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Malvales › Malvaceae › Malvoideae › Gossypium

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Chloroplast Potential
	Chain	44 – 405	362	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic		PRO_0000000602

Sequences

	Sequence		Length	Mass (Da)
	Q6SJV8-1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 55BC9EEB09C0A807	FASTA	405	47,008
	10 20 30 40 50 60 MATEMALVKP ISKFSTSSPI FSNSRYGKFT TVRMSSTSQS TTKAAAKGGK KAAKTAIKET 70 80 90 100 110 120 LLTPRFYTTD FDEMEALFNT EINKNLNQSE FESLLQEFKT DYNQTHFVRN KEFKEAADKI 130 140 150 160 170 180 DGPLRQIFVE FSERSCTAEF SGFLLYKELG RRLKKTNPVV AEIFSLMSRD EARHAGFLNK 190 200 210 220 230 240 GLSDFNLALD LGFLTKARKY TFFKPKFIFY ATYLSEKIGY WRYITIYRHL KENPEYQCYP 250 260 270 280 290 300 IFKYFENWCQ DENRHGDFFS ALLKAQPQFL NDWKAKLWSR FFCLSVYVTM YLNDCQRTAF 310 320 330 340 350 360 YEGIGLDTKE FDMHVIIETN RTTARIFPAV LDVENPEFKR RLDKMVEINE QLLAVGETSD 370 380 390 400 IPLVKNLKRI PLIAALASEL LATYLMPPIE SGSVDFAEFE PQLVY

References

[1]	"Cloning and characterization of GhZIP." Hua X., Liu N., Liu H., Yang Y., Shan D., Zhang Y., Guo Y., Zhang M., Wu C., Li X., Zheng C. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY456957 mRNA. Translation: AAR20445.2. Different initiation.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.81. 1857.
Family and domain databases
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CRD1_GOSHI

Accession

Primary (citable) accession number: Q6SJV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)