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Protein

CMRF35-like molecule 1

Gene

Cd300lf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation. Induces macrophage cell death upon engagement.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei241 – 2411Phosphatase-bindingBy similarity

GO - Biological processi

  • osteoclast differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
CMRF35-like molecule 1
Short name:
CLM-1
Alternative name(s):
CD300 antigen-like family member F
Leukocyte mono-Ig-like receptor 3
Myeloid-associated immunoglobulin-like receptor 5
Short name:
MAIR-5
Short name:
MAIR-V
CD_antigen: CD300f
Gene namesi
Name:Cd300lf
Synonyms:Clm1, Lmir3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2442359. Cd300lf.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 193174ExtracellularSequence analysisAdd
BLAST
Transmembranei194 – 21421HelicalSequence analysisAdd
BLAST
Topological domaini215 – 337123CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 337318CMRF35-like molecule 1PRO_0000247826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 1091 Publication
Disulfide bondi55 ↔ 631 Publication

Post-translational modificationi

Phosphorylated on tyrosine.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiQ6SJQ7.
PaxDbiQ6SJQ7.
PRIDEiQ6SJQ7.

PTM databases

PhosphoSiteiQ6SJQ7.

Expressioni

Tissue specificityi

Expressed in myeloid cells. Present on the surface of macrophages (at protein level).2 Publications

Gene expression databases

BgeeiQ6SJQ7.
CleanExiMM_CD300LF.
ExpressionAtlasiQ6SJQ7. baseline and differential.
GenevisibleiQ6SJQ7. MM.

Interactioni

Subunit structurei

Interacts with PTPN6/SHP-1 in a tyrosine phosphorylation dependent manner.1 Publication

Protein-protein interaction databases

BioGridi232945. 1 interaction.
IntActiQ6SJQ7. 1 interaction.
STRINGi10090.ENSMUSP00000102171.

Structurei

Secondary structure

1
337
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 243Combined sources
Beta strandi27 – 326Combined sources
Beta strandi37 – 437Combined sources
Helixi45 – 473Combined sources
Beta strandi52 – 598Combined sources
Helixi60 – 623Combined sources
Beta strandi64 – 685Combined sources
Beta strandi71 – 733Combined sources
Beta strandi76 – 783Combined sources
Beta strandi81 – 866Combined sources
Turni87 – 904Combined sources
Beta strandi91 – 966Combined sources
Helixi101 – 1033Combined sources
Beta strandi105 – 1117Combined sources
Beta strandi114 – 1163Combined sources
Beta strandi119 – 1279Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZOXX-ray2.10A20-138[»]
ProteinModelPortaliQ6SJQ7.
SMRiQ6SJQ7. Positions 20-128.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6SJQ7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 125106Ig-like V-typeAdd
BLAST

Sequence similaritiesi

Belongs to the CD300 family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IZ4I. Eukaryota.
ENOG410YT8T. LUCA.
GeneTreeiENSGT00470000042273.
HOGENOMiHOG000231065.
HOVERGENiHBG050999.
InParanoidiQ6SJQ7.
OMAiEGDLCYA.
OrthoDBiEOG78PVBM.
PhylomeDBiQ6SJQ7.
TreeFamiTF334441.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6SJQ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHLSLLVPFL FWITGCCTAE DPVTGPEEVS GQEQGSLTVQ CRYTSGWKDY
60 70 80 90 100
KKYWCQGVPQ RSCKTLVETD ASEQLVKKNR VSIRDNQRDF IFTVTMEDLR
110 120 130 140 150
MSDAGIYWCG ITKGGLDPMF KVTVNIGPAI QVPITVPTMP PITSTTTIFT
160 170 180 190 200
VTTTVKETSM FPTLTSYYSD NGHGGGDSGG GEDGVGDGFL DLSVLLPVIS
210 220 230 240 250
AVLLLLLLVA SLFAWRMVRR QKKAAGPPSE QAQSLEGDLC YADLSLKQPR
260 270 280 290 300
TSPGSSWKKG SSMSSSGKDH QEEVEYVTMA PFPREEVSYA ALTLAGLGQE
310 320 330
PTYGNTGCPI THVPRTGLEE ETTEYSSIRR PLPAAMP
Length:337
Mass (Da):36,715
Last modified:July 5, 2004 - v1
Checksum:i0C6EB6E666223CD5
GO
Isoform 2 (identifier: Q6SJQ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     129-135: Missing.

Note: No experimental confirmation available.
Show »
Length:330
Mass (Da):35,992
Checksum:iBB52F7F1ED34558C
GO
Isoform 3 (identifier: Q6SJQ7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-265: Missing.

Note: No experimental confirmation available.
Show »
Length:283
Mass (Da):30,791
Checksum:iC6AC9719C7FD5661
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201E → Q in AB292061 (PubMed:17438331).Curated
Sequence conflicti37 – 371L → V in AAM19096 (Ref. 3) Curated
Sequence conflicti56 – 6510QGVPQRSCKT → RGAYWKSCEI in AB292061 (PubMed:17438331).Curated
Sequence conflicti71 – 711A → K in AB292061 (PubMed:17438331).Curated
Sequence conflicti114 – 1163GGL → AGP in AB292061 (PubMed:17438331).Curated
Sequence conflicti123 – 1231T → A in AB292061 (PubMed:17438331).Curated
Sequence conflicti166 – 1661S → G in AB292061 (PubMed:17438331).Curated
Sequence conflicti337 – 3371P → H in AB292061 (PubMed:17438331).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei129 – 1357Missing in isoform 2. 1 PublicationVSP_020066
Alternative sequencei212 – 26554Missing in isoform 3. 1 PublicationVSP_020067Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY457047 mRNA. Translation: AAR27938.1.
AB292061 mRNA. No translation available.
AF251703 mRNA. Translation: AAM19096.1.
AL606487 Genomic DNA. Translation: CAM21607.1.
AL606487 Genomic DNA. Translation: CAM21609.1.
BC057864 mRNA. Translation: AAH57864.1.
CCDSiCCDS25619.1. [Q6SJQ7-2]
CCDS48980.1. [Q6SJQ7-1]
RefSeqiNP_001162624.1. NM_001169153.1. [Q6SJQ7-1]
NP_663609.2. NM_145634.3. [Q6SJQ7-2]
UniGeneiMm.277387.

Genome annotation databases

EnsembliENSMUST00000051264; ENSMUSP00000053983; ENSMUSG00000047798. [Q6SJQ7-2]
ENSMUST00000106561; ENSMUSP00000102171; ENSMUSG00000047798. [Q6SJQ7-1]
GeneIDi246746.
KEGGimmu:246746.
UCSCiuc007mgn.2. mouse. [Q6SJQ7-1]
uc011yhh.1. mouse. [Q6SJQ7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY457047 mRNA. Translation: AAR27938.1.
AB292061 mRNA. No translation available.
AF251703 mRNA. Translation: AAM19096.1.
AL606487 Genomic DNA. Translation: CAM21607.1.
AL606487 Genomic DNA. Translation: CAM21609.1.
BC057864 mRNA. Translation: AAH57864.1.
CCDSiCCDS25619.1. [Q6SJQ7-2]
CCDS48980.1. [Q6SJQ7-1]
RefSeqiNP_001162624.1. NM_001169153.1. [Q6SJQ7-1]
NP_663609.2. NM_145634.3. [Q6SJQ7-2]
UniGeneiMm.277387.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZOXX-ray2.10A20-138[»]
ProteinModelPortaliQ6SJQ7.
SMRiQ6SJQ7. Positions 20-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232945. 1 interaction.
IntActiQ6SJQ7. 1 interaction.
STRINGi10090.ENSMUSP00000102171.

PTM databases

PhosphoSiteiQ6SJQ7.

Proteomic databases

MaxQBiQ6SJQ7.
PaxDbiQ6SJQ7.
PRIDEiQ6SJQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051264; ENSMUSP00000053983; ENSMUSG00000047798. [Q6SJQ7-2]
ENSMUST00000106561; ENSMUSP00000102171; ENSMUSG00000047798. [Q6SJQ7-1]
GeneIDi246746.
KEGGimmu:246746.
UCSCiuc007mgn.2. mouse. [Q6SJQ7-1]
uc011yhh.1. mouse. [Q6SJQ7-2]

Organism-specific databases

CTDi146722.
MGIiMGI:2442359. Cd300lf.

Phylogenomic databases

eggNOGiENOG410IZ4I. Eukaryota.
ENOG410YT8T. LUCA.
GeneTreeiENSGT00470000042273.
HOGENOMiHOG000231065.
HOVERGENiHBG050999.
InParanoidiQ6SJQ7.
OMAiEGDLCYA.
OrthoDBiEOG78PVBM.
PhylomeDBiQ6SJQ7.
TreeFamiTF334441.

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

EvolutionaryTraceiQ6SJQ7.
NextBioi387298.
PROiQ6SJQ7.
SOURCEiSearch...

Gene expression databases

BgeeiQ6SJQ7.
CleanExiMM_CD300LF.
ExpressionAtlasiQ6SJQ7. baseline and differential.
GenevisibleiQ6SJQ7. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CMRF-35-like molecule-1, a novel mouse myeloid receptor, can inhibit osteoclast formation."
    Chung D.-H., Humphrey M.B., Nakamura M.C., Ginzinger D.G., Seaman W.E., Daws M.R.
    J. Immunol. 171:6541-6548(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH PTPN6.
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
    Strain: CBA/J.
  3. Zhang W., Wan T., Li N., Chen T., Cao X.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: 129.
    Tissue: Mammary gland.
  6. "Caspase-independent cell death by CD300LF (MAIR-V), an inhibitory immunoglobulin-like receptor on myeloid cells."
    Can I., Tahara-Hanaoka S., Hitomi K., Nakano T., Nakahashi-Oda C., Kurita N., Honda S., Shibuya K., Shibuya A.
    J. Immunol. 180:207-213(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  7. "Crystal structure of the mouse CLM-1 ectodomain."
    Midwest center for structural genomics (MCSG)
    Submitted (MAY-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 20-138, DISULFIDE BONDS.

Entry informationi

Entry nameiCLM1_MOUSE
AccessioniPrimary (citable) accession number: Q6SJQ7
Secondary accession number(s): A2A6Z1
, A2A6Z3, Q6PEU7, Q8K4V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 5, 2004
Last modified: April 13, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.