Reviewed, UniProtKB/Swiss-Prot Q6SJ95 (TBPL2_MOUSE)

Names and origin

Protein names

Recommended name:
    TATA box-binding protein-like protein 2
      Short name=TBP-like protein 2
Alternative name(s):
    TATA box-binding protein-related factor 3
      Short name=TBP-related factor 3

Gene names

Name:	Tbpl2
Synonyms:	Tbp2, Trf3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor required in complex with TAF3 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process. Ref.7
Subunit structure	Interacts with TAF3. Ref.7
Subcellular location	Cytoplasm. Nucleus. Note: Present in the cytoplasm during cytokenesis By similarity. UniProtKB Q6SJ96
Tissue specificity	Expressed in myotubes and myofibers (at protein level). Expressed in a wide variety of tissues with highest levels in heart, lung, liver, uterus and placenta and especially the gonads. Expression is higher in the ovary than the testis, and within the ovary expression is localized to the oocytes. Ref.7 Ref.1 Ref.5 Ref.6
Sequence similarities	Belongs to the TBP family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription, DNA-dependent Inferred from direct assay. Source: MGI transcription initiation from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	RNA polymerase II transcription factor activity Inferred from electronic annotation. Source: InterPro protein binding Ref.7 Inferred from physical interaction. Source: IntAct transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Taf3	Q5HZG4	2	EBI-1571412,EBI-1561080

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	TATA box-binding protein-like protein 2		PRO_0000318119
Experimental info
	Sequence conflict	294	1	Missing in DAA06033. Ref.2
	Sequence conflict	294	1	Missing in CAM19021. Ref.3
	Sequence conflict	294	1	Missing in AAI00306. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q6SJ95-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A436F5AE830B5CCB	FASTA	350	38,967
	10 20 30 40 50 60 MVGSLIMEED IYLDLFLDPY TIQDDFPPAM SQLFSPGVPL DMHSLPSNPE TVFHPHLGGV 70 80 90 100 110 120 KKASTDFSSV DLSFLPDELT QENRDQTVTG NKLASEESCR TRDRQSQLQL PDEHGSELNL 130 140 150 160 170 180 NSNSSPDPQS CLCFDDAHSN QPSPETPNSN ALPVALIASM MPMNPVPGFS GIVPQLQNVV 190 200 210 220 230 240 STANLACKLD LRKIALNAKN TEYNPKRFAA VIMRIREPRT TALIFSSGKV VCTGAKSEEE 250 260 270 280 290 300 SRLAARKYAR VVQKLGFPVR FFNFKIQNMV GSCDVKFPIR LEILALTHRQ FSSSYEPELF 310 320 330 340 350 PGLIYKMVKP QVVLLIFASG KVVLTGAKER SEIYEAFENM YPILESFKKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TRF3, a TATA-box-binding protein-related factor, is vertebrate-specific and widely expressed." Persengiev S.P., Zhu X., Dixit B.L., Maston G.A., Kittler E.L.W., Green M.R. Proc. Natl. Acad. Sci. U.S.A. 100:14887-14891(2003) [PubMed: 14634207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Swiss Webster.
[2]	"Genomics, evolution, and expression of TBPL2, a member of the TBP family." Di Pietro C., Ragusa M., Duro L., Guglielmino M.R., Barbagallo D., Carnemolla A., Lagana A., Buffa P., Angelica R., Rinaldi A., Calafato M.S., Milicia I., Caserta C., Giugno R., Pulvirenti A., Giunta V., Rapisarda A., Di Pietro V. , Grillo A., Messina A., Ferro A., Grzeschik K.H., Purrello M. DNA Cell Biol. 26:369-385(2007) [PubMed: 17570761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57B16/J. Tissue: Oocyte.
[5]	"TBP2, a vertebrate-specific member of the TBP family, is required in embryonic development of zebrafish." Bartfai R., Balduf C., Hilton T., Rathmann Y., Hadzhiev Y., Tora L., Orban L., Mueller F. Curr. Biol. 14:593-598(2004) [PubMed: 15062100] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Developmental and cell type-specific regulation of core promoter transcription factors in germ cells of frogs and mice." Xiao L., Kim M., DeJong J. Gene Expr. Patterns 6:409-419(2006) [PubMed: 16412700] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Switching of the core transcription machinery during myogenesis." Deato M.D.E., Tjian R. Genes Dev. 21:2137-2149(2007) [PubMed: 17704303] [Abstract] Cited for: FUNCTION, INTERACTION WITH TAF3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY457924 mRNA. Translation: AAR24282.1. BK005773 mRNA. Translation: DAA06033.1. AL844838 Genomic DNA. Translation: CAM19020.1. AL844838 Genomic DNA. Translation: CAM19021.1. BC100305 mRNA. Translation: AAI00306.1. BC119160 mRNA. Translation: AAI19161.1.
IPI	IPI00756407.
RefSeq	NP_951014.1.
UniGene	Mm.301625
3D structure databases
HSSP	HSSP built from PDB template 1JFI based on UniProtKB P20226.
SMR	Q6SJ95. Positions 170-349.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6SJ95. 2 interactions.
STRING	Q6SJ95.
Proteomic databases
PRIDE	Q6SJ95.
Genome annotation databases
Ensembl	ENSMUST00000080453; ENSMUSP00000079309; ENSMUSG00000061809; Mus musculus. [Genome view]
GeneID	227606.
KEGG	mmu:227606.
NMPDR	fig|10090.3.peg.5522.
UCSC	uc008iol.1. mouse.
Organism-specific databases
CTD	227606.
MGI	MGI:2684058. Tbpl2.
Phylogenomic databases
HOGENOM	Q6SJ95.
HOVERGEN	Q6SJ95.
OMA	LYLDQCA
OrthoDB	EOG9K0T76
Gene expression databases
Bgee	Q6SJ95.
CleanEx	MM_TBPL2.
Genevestigator	Q6SJ95.
Family and domain databases
InterPro	IPR012295. b_Adaptin_TBP_C. IPR000814. TBP. IPR012294. TFIID_C/glycos_N. [Graphical view]
Gene3D	G3DSA:3.30.310.10. b_Adaptin_TBP_C. 2 hits.
PANTHER	PTHR10126. TBP. 1 hit.
Pfam	PF00352. TBP. 2 hits. [Graphical view]
PRINTS	PR00686. TIFACTORIID.
PROSITE	PS00351. TFIID. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	378650.
SOURCE	Search...

Entry information

Entry name

TBPL2_MOUSE

Accession

Primary (citable) accession number: Q6SJ95
Secondary accession number(s): A2AQK6, Q498A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 5, 2004
Last modified:	November 24, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)