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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

UCHL1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).By similarity

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90NucleophilePROSITE-ProRule annotation1
Active sitei161Proton donorPROSITE-ProRule annotation1
Sitei176Important for enzyme activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ligase, Protease, Thiol protease
Biological processUbl conjugation pathway

Protein family/group databases

MEROPSiC12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Ubiquitin thioesterase L1
Gene namesi
Name:UCHL1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002110591 – 220Ubiquitin carboxyl-terminal hydrolase isozyme L1Add BLAST220
PropeptideiPRO_0000414314221 – 223Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei125PhosphoserineBy similarity1
Lipidationi220S-farnesyl cysteineBy similarity1

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PeptideAtlasiQ6SEG5.
PRIDEiQ6SEG5.

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 and SNCA (By similarity).By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ6SEG5.
SMRiQ6SEG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 10Interaction with ubiquitinBy similarity6
Regioni211 – 216Interaction with ubiquitinBy similarity6

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000046640.
HOVERGENiHBG075483.
InParanoidiQ6SEG5.
KOiK05611.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiView protein in InterPro
IPR001578. Peptidase_C12_UCH.
IPR036959. Peptidase_C12_UCH_sf.
IPR030297. UCHL1.
PANTHERiPTHR10589. PTHR10589. 1 hit.
PTHR10589:SF19. PTHR10589:SF19. 1 hit.
PfamiView protein in Pfam
PF01088. Peptidase_C12. 1 hit.
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiView protein in PROSITE
PS00140. UCH_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6SEG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLKPMEINP EMLNKVLTRL GVAGHWRFAD VLGLEEESLG SVPAPACALL
60 70 80 90 100
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA
110 120 130 140 150
NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDAVAQEG
160 170 180 190 200
QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DSLLQDAAKV
210 220
CREFTEREQG EVRFSAVALC KAA
Length:223
Mass (Da):24,859
Last modified:July 5, 2004 - v1
Checksum:i1023BB6B78AECEEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY459532 mRNA. Translation: AAR22407.1.
RefSeqiNP_998928.1. NM_213763.2.
UniGeneiSsc.2073.

Genome annotation databases

EnsembliENSSSCT00000024016; ENSSSCP00000020119; ENSSSCG00000022945.
GeneIDi396637.
KEGGissc:396637.

Similar proteinsi

Entry informationi

Entry nameiUCHL1_PIG
AccessioniPrimary (citable) accession number: Q6SEG5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: November 22, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families