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Q6SEG5

- UCHL1_PIG

UniProt

Q6SEG5 - UCHL1_PIG

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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene
UCHL1
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1 – 11Susceptible to oxidation By similarity
Sitei6 – 61Susceptible to oxidation By similarity
Sitei12 – 121Susceptible to oxidation By similarity
Active sitei90 – 901Nucleophile By similarity
Active sitei161 – 1611Proton donor By similarity
Sitei176 – 1761Important for enzyme activity By similarity
Sitei179 – 1791Susceptible to oxidation By similarity
Sitei220 – 2201Susceptible to oxidation By similarity

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Ubiquitin thioesterase L1
Gene namesi
Name:UCHL1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ubiquitin carboxyl-terminal hydrolase isozyme L1PRO_0000211059Add
BLAST
Propeptidei221 – 2233Removed in mature form By similarityPRO_0000414314

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi220 – 2201S-farnesyl cysteine By similarity

Post-translational modificationi

O-glycosylated By similarity.

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Prenylation

Proteomic databases

PaxDbiQ6SEG5.

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000009376.

Structurei

3D structure databases

ProteinModelPortaliQ6SEG5.
SMRiQ6SEG5. Positions 1-223.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 106Interaction with ubiquitin By similarity
Regioni151 – 1566Interaction with ubiquitin By similarity
Regioni211 – 2166Interaction with ubiquitin By similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.

Phylogenomic databases

eggNOGiopiNOG07658.
HOVERGENiHBG075483.
KOiK05611.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6SEG5-1 [UniParc]FASTAAdd to Basket

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MQLKPMEINP EMLNKVLTRL GVAGHWRFAD VLGLEEESLG SVPAPACALL    50
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA 100
NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDAVAQEG 150
QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DSLLQDAAKV 200
CREFTEREQG EVRFSAVALC KAA 223
Length:223
Mass (Da):24,859
Last modified:July 5, 2004 - v1
Checksum:i1023BB6B78AECEEC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY459532 mRNA. Translation: AAR22407.1.
RefSeqiNP_998928.1. NM_213763.2.
UniGeneiSsc.2073.

Genome annotation databases

GeneIDi396637.
KEGGissc:396637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY459532 mRNA. Translation: AAR22407.1 .
RefSeqi NP_998928.1. NM_213763.2.
UniGenei Ssc.2073.

3D structure databases

ProteinModelPortali Q6SEG5.
SMRi Q6SEG5. Positions 1-223.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000009376.

Protein family/group databases

MEROPSi C12.001.

Proteomic databases

PaxDbi Q6SEG5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 396637.
KEGGi ssc:396637.

Organism-specific databases

CTDi 7345.

Phylogenomic databases

eggNOGi opiNOG07658.
HOVERGENi HBG075483.
KOi K05611.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PRINTSi PR00707. UBCTHYDRLASE.
PROSITEi PS00140. UCH_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of ubiquitin carboxyl-terminal hydrolase in pig."
    Tian X., Li J., Chen Y.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Longissimus muscle.

Entry informationi

Entry nameiUCHL1_PIG
AccessioniPrimary (citable) accession number: Q6SEG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8 By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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