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Q6SEG5

- UCHL1_PIG

UniProt

Q6SEG5 - UCHL1_PIG

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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

UCHL1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901NucleophilePROSITE-ProRule annotation
Active sitei161 – 1611Proton donorPROSITE-ProRule annotation
Sitei176 – 1761Important for enzyme activityBy similarity

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Ubiquitin thioesterase L1
Gene namesi
Name:UCHL1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Cytoplasm By similarity. Endoplasmic reticulum membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ubiquitin carboxyl-terminal hydrolase isozyme L1PRO_0000211059Add
BLAST
Propeptidei221 – 2233Removed in mature formBy similarityPRO_0000414314

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi220 – 2201S-farnesyl cysteineBy similarity

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Prenylation

Proteomic databases

PaxDbiQ6SEG5.

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 and SNCA (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000009376.

Structurei

3D structure databases

ProteinModelPortaliQ6SEG5.
SMRiQ6SEG5. Positions 1-223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 106Interaction with ubiquitinBy similarity
Regioni151 – 1566Interaction with ubiquitinBy similarity
Regioni211 – 2166Interaction with ubiquitinBy similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiopiNOG07658.
HOVERGENiHBG075483.
InParanoidiQ6SEG5.
KOiK05611.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6SEG5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQLKPMEINP EMLNKVLTRL GVAGHWRFAD VLGLEEESLG SVPAPACALL
60 70 80 90 100
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA
110 120 130 140 150
NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDAVAQEG
160 170 180 190 200
QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DSLLQDAAKV
210 220
CREFTEREQG EVRFSAVALC KAA
Length:223
Mass (Da):24,859
Last modified:July 5, 2004 - v1
Checksum:i1023BB6B78AECEEC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY459532 mRNA. Translation: AAR22407.1.
RefSeqiNP_998928.1. NM_213763.2.
UniGeneiSsc.2073.

Genome annotation databases

GeneIDi396637.
KEGGissc:396637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY459532 mRNA. Translation: AAR22407.1 .
RefSeqi NP_998928.1. NM_213763.2.
UniGenei Ssc.2073.

3D structure databases

ProteinModelPortali Q6SEG5.
SMRi Q6SEG5. Positions 1-223.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000009376.

Protein family/group databases

MEROPSi C12.001.

Proteomic databases

PaxDbi Q6SEG5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 396637.
KEGGi ssc:396637.

Organism-specific databases

CTDi 7345.

Phylogenomic databases

eggNOGi opiNOG07658.
HOVERGENi HBG075483.
InParanoidi Q6SEG5.
KOi K05611.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PRINTSi PR00707. UBCTHYDRLASE.
PROSITEi PS00140. UCH_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of ubiquitin carboxyl-terminal hydrolase in pig."
    Tian X., Li J., Chen Y.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Longissimus muscle.

Entry informationi

Entry nameiUCHL1_PIG
AccessioniPrimary (citable) accession number: Q6SEG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3