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Q6SA08

- TSSK4_HUMAN

UniProt

Q6SA08 - TSSK4_HUMAN

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Protein
Testis-specific serine/threonine-protein kinase 4
Gene
TSSK4, C14orf20, STK22E, TSSK5
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May be involved in a signaling pathway during male germ cell development or mature sperm function By similarity. Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.

Enzyme regulationi

Activated by phosphorylation on Thr-197, potentially by autophosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATP By similarity
Active sitei148 – 1481Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein binding Source: IntAct
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. positive regulation of CREB transcription factor activity Source: UniProtKB
  4. protein phosphorylation Source: UniProtKB
  5. spermatogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
SignaLinkiQ6SA08.

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 4 (EC:2.7.11.1)
Short name:
TSK-4
Short name:
TSSK-4
Short name:
Testis-specific kinase 4
Alternative name(s):
Serine/threonine-protein kinase 22E
Gene namesi
Name:TSSK4
Synonyms:C14orf20, STK22E, TSSK5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:19825. TSSK4.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134908318.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Testis-specific serine/threonine-protein kinase 4
PRO_0000086772Add
BLAST

Post-translational modificationi

Autophosphorylated By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6SA08.
PRIDEiQ6SA08.

PTM databases

PhosphoSiteiQ6SA08.

Expressioni

Tissue specificityi

Expressed only in the testis.1 Publication

Gene expression databases

ArrayExpressiQ6SA08.
BgeeiQ6SA08.
CleanExiHS_TSSK4.
GenevestigatoriQ6SA08.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CREB1P162205EBI-1202583,EBI-711855

Protein-protein interaction databases

BioGridi129625. 1 interaction.
IntActiQ6SA08. 1 interaction.
STRINGi9606.ENSP00000287913.

Structurei

3D structure databases

ProteinModelPortaliQ6SA08.
SMRiQ6SA08. Positions 18-321.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 293269Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
KOiK08811.
OMAiIIKDPWV.
OrthoDBiEOG7P8P8F.
PhylomeDBiQ6SA08.
TreeFamiTF105333.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6SA08-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGKGDVLEAA PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM    50
VAVKIISKKK ASDDYLNKFL PREIQVMKVL RHKYLINFYR AIESTSRVYI 100
ILELAQGGDV LEWIQRYGAC SEPLAGKWFS QLTLGIAYLH SKSIVHRDLK 150
LENLLLDKWE NVKISDFGFA KMVPSNQPVG CSPSYRQVNC FSHLSQTYCG 200
SFAYACPEIL RGLPYNPFLS DTWSMGVILY TLVVAHLPFD DTNLKKLLRE 250
TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD SWVLKFQPEQ 300
PTHEIRLLEA MCQLHNTTKQ HQSLQITT 328
Length:328
Mass (Da):37,454
Last modified:July 5, 2004 - v1
Checksum:iC78502FF6F71754C
GO
Isoform 2 (identifier: Q6SA08-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: R → RLMPSLSAAGR

Show »
Length:338
Mass (Da):38,439
Checksum:i8E3CC56DBF1B5F78
GO
Isoform 3 (identifier: Q6SA08-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: No experimental confirmation available.

Show »
Length:252
Mass (Da):29,034
Checksum:i0142331DA68E06C9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331H → Y.1 Publication
Corresponds to variant rs36036137 [ dbSNP | Ensembl ].
VAR_041247
Natural varianti89 – 891Y → C.1 Publication
Corresponds to variant rs34083933 [ dbSNP | Ensembl ].
VAR_041248
Natural varianti145 – 1451V → M.1 Publication
Corresponds to variant rs35468205 [ dbSNP | Ensembl ].
VAR_041249
Natural varianti196 – 1961Q → R.1 Publication
Corresponds to variant rs1270764 [ dbSNP | Ensembl ].
VAR_041250
Natural varianti327 – 3271T → M.1 Publication
Corresponds to variant rs35244223 [ dbSNP | Ensembl ].
VAR_041251

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7676Missing in isoform 3.
VSP_023559Add
BLAST
Alternative sequencei147 – 1471R → RLMPSLSAAGR in isoform 2.
VSP_023560

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151Q → R in BAC85482. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY461663 mRNA. Translation: AAS17971.1.
AK131042 mRNA. Translation: BAC85482.1.
BC111088 mRNA. Translation: AAI11089.1.
CCDSiCCDS53890.1. [Q6SA08-2]
CCDS9618.1. [Q6SA08-1]
RefSeqiNP_001171668.1. NM_001184739.1. [Q6SA08-2]
NP_777604.2. NM_174944.3. [Q6SA08-1]
UniGeneiHs.314432.

Genome annotation databases

EnsembliENST00000287913; ENSP00000287913; ENSG00000139908. [Q6SA08-1]
ENST00000339917; ENSP00000339179; ENSG00000139908. [Q6SA08-2]
ENST00000556621; ENSP00000452054; ENSG00000139908. [Q6SA08-3]
GeneIDi283629.
KEGGihsa:283629.
UCSCiuc001wne.3. human. [Q6SA08-1]
uc001wnh.3. human. [Q6SA08-2]

Polymorphism databases

DMDMi62287888.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY461663 mRNA. Translation: AAS17971.1 .
AK131042 mRNA. Translation: BAC85482.1 .
BC111088 mRNA. Translation: AAI11089.1 .
CCDSi CCDS53890.1. [Q6SA08-2 ]
CCDS9618.1. [Q6SA08-1 ]
RefSeqi NP_001171668.1. NM_001184739.1. [Q6SA08-2 ]
NP_777604.2. NM_174944.3. [Q6SA08-1 ]
UniGenei Hs.314432.

3D structure databases

ProteinModelPortali Q6SA08.
SMRi Q6SA08. Positions 18-321.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 129625. 1 interaction.
IntActi Q6SA08. 1 interaction.
STRINGi 9606.ENSP00000287913.

PTM databases

PhosphoSitei Q6SA08.

Polymorphism databases

DMDMi 62287888.

Proteomic databases

PaxDbi Q6SA08.
PRIDEi Q6SA08.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000287913 ; ENSP00000287913 ; ENSG00000139908 . [Q6SA08-1 ]
ENST00000339917 ; ENSP00000339179 ; ENSG00000139908 . [Q6SA08-2 ]
ENST00000556621 ; ENSP00000452054 ; ENSG00000139908 . [Q6SA08-3 ]
GeneIDi 283629.
KEGGi hsa:283629.
UCSCi uc001wne.3. human. [Q6SA08-1 ]
uc001wnh.3. human. [Q6SA08-2 ]

Organism-specific databases

CTDi 283629.
GeneCardsi GC14P024674.
HGNCi HGNC:19825. TSSK4.
MIMi 610711. gene.
neXtProti NX_Q6SA08.
PharmGKBi PA134908318.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233016.
HOVERGENi HBG101110.
KOi K08811.
OMAi IIKDPWV.
OrthoDBi EOG7P8P8F.
PhylomeDBi Q6SA08.
TreeFami TF105333.

Enzyme and pathway databases

BRENDAi 2.7.11.1. 2681.
SignaLinki Q6SA08.

Miscellaneous databases

GenomeRNAii 283629.
NextBioi 94101.
PROi Q6SA08.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6SA08.
Bgeei Q6SA08.
CleanExi HS_TSSK4.
Genevestigatori Q6SA08.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway."
    Chen X., Lin G., Wei Y., Hexige S., Niu Y., Liu L., Yang C., Yu L.
    Biochem. Biophys. Res. Commun. 333:742-749(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  4. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] TYR-33; CYS-89; MET-145; ARG-196 AND MET-327.

Entry informationi

Entry nameiTSSK4_HUMAN
AccessioniPrimary (citable) accession number: Q6SA08
Secondary accession number(s): Q2TA60, Q6ZNM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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