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Q6SA08 (TSSK4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Testis-specific serine/threonine-protein kinase 4

Short name=TSK-4
Short name=TSSK-4
Short name=Testis-specific kinase 4
EC=2.7.11.1
Alternative name(s):
Serine/threonine-protein kinase 22E
Gene names
Name:TSSK4
Synonyms:C14orf20, STK22E, TSSK5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in a signaling pathway during male germ cell development or mature sperm function By similarity. Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Enzyme regulation

Activated by phosphorylation on Thr-197, potentially by autophosphorylation By similarity.

Tissue specificity

Expressed only in the testis. Ref.1

Post-translational modification

Autophosphorylated By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CREB1P162205EBI-1202583,EBI-711855

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6SA08-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6SA08-2)

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: R → RLMPSLSAAGR
Isoform 3 (identifier: Q6SA08-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Testis-specific serine/threonine-protein kinase 4
PRO_0000086772

Regions

Domain25 – 293269Protein kinase
Nucleotide binding31 – 399ATP By similarity

Sites

Active site1481Proton acceptor By similarity
Binding site541ATP By similarity

Natural variations

Alternative sequence1 – 7676Missing in isoform 3.
VSP_023559
Alternative sequence1471R → RLMPSLSAAGR in isoform 2.
VSP_023560
Natural variant331H → Y. Ref.4
Corresponds to variant rs36036137 [ dbSNP | Ensembl ].
VAR_041247
Natural variant891Y → C. Ref.4
Corresponds to variant rs34083933 [ dbSNP | Ensembl ].
VAR_041248
Natural variant1451V → M. Ref.4
Corresponds to variant rs35468205 [ dbSNP | Ensembl ].
VAR_041249
Natural variant1961Q → R. Ref.4
Corresponds to variant rs1270764 [ dbSNP | Ensembl ].
VAR_041250
Natural variant3271T → M. Ref.4
Corresponds to variant rs35244223 [ dbSNP | Ensembl ].
VAR_041251

Experimental info

Sequence conflict1151Q → R in BAC85482. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C78502FF6F71754C

FASTA32837,454
        10         20         30         40         50         60 
MGKGDVLEAA PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM VAVKIISKKK 

        70         80         90        100        110        120 
ASDDYLNKFL PREIQVMKVL RHKYLINFYR AIESTSRVYI ILELAQGGDV LEWIQRYGAC 

       130        140        150        160        170        180 
SEPLAGKWFS QLTLGIAYLH SKSIVHRDLK LENLLLDKWE NVKISDFGFA KMVPSNQPVG 

       190        200        210        220        230        240 
CSPSYRQVNC FSHLSQTYCG SFAYACPEIL RGLPYNPFLS DTWSMGVILY TLVVAHLPFD 

       250        260        270        280        290        300 
DTNLKKLLRE TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD SWVLKFQPEQ 

       310        320 
PTHEIRLLEA MCQLHNTTKQ HQSLQITT 

« Hide

Isoform 2 [UniParc].

Checksum: 8E3CC56DBF1B5F78
Show »

FASTA33838,439
Isoform 3 [UniParc].

Checksum: 0142331DA68E06C9
Show »

FASTA25229,034

References

« Hide 'large scale' references
[1]"TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway."
Chen X., Lin G., Wei Y., Hexige S., Niu Y., Liu L., Yang C., Yu L.
Biochem. Biophys. Res. Commun. 333:742-749(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Tissue: Testis.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] TYR-33; CYS-89; MET-145; ARG-196 AND MET-327.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY461663 mRNA. Translation: AAS17971.1.
AK131042 mRNA. Translation: BAC85482.1.
BC111088 mRNA. Translation: AAI11089.1.
RefSeqNP_001171668.1. NM_001184739.1.
NP_777604.2. NM_174944.3.
UniGeneHs.314432.

3D structure databases

ProteinModelPortalQ6SA08.
SMRQ6SA08. Positions 25-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid129625. 1 interaction.
IntActQ6SA08. 1 interaction.
STRING9606.ENSP00000287913.

PTM databases

PhosphoSiteQ6SA08.

Polymorphism databases

DMDM62287888.

Proteomic databases

PaxDbQ6SA08.
PRIDEQ6SA08.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000287913; ENSP00000287913; ENSG00000139908. [Q6SA08-1]
ENST00000339917; ENSP00000339179; ENSG00000139908. [Q6SA08-2]
ENST00000556621; ENSP00000452054; ENSG00000139908. [Q6SA08-3]
GeneID283629.
KEGGhsa:283629.
UCSCuc001wne.3. human. [Q6SA08-1]
uc001wnh.3. human. [Q6SA08-2]

Organism-specific databases

CTD283629.
GeneCardsGC14P024674.
HGNCHGNC:19825. TSSK4.
MIM610711. gene.
neXtProtNX_Q6SA08.
PharmGKBPA134908318.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233016.
HOVERGENHBG101110.
KOK08811.
OMAIIKDPWV.
OrthoDBEOG7P8P8F.
PhylomeDBQ6SA08.
TreeFamTF105333.

Enzyme and pathway databases

BRENDA2.7.11.1. 2681.
SignaLinkQ6SA08.

Gene expression databases

ArrayExpressQ6SA08.
BgeeQ6SA08.
CleanExHS_TSSK4.
GenevestigatorQ6SA08.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi283629.
NextBio94101.
PROQ6SA08.
SOURCESearch...

Entry information

Entry nameTSSK4_HUMAN
AccessionPrimary (citable) accession number: Q6SA08
Secondary accession number(s): Q2TA60, Q6ZNM2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM