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Protein

Testis-specific serine/threonine-protein kinase 4

Gene

TSSK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Activated by phosphorylation on Thr-197, potentially by autophosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei148Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS13803-MONOMER.
BRENDAi2.7.11.1. 2681.
SignaLinkiQ6SA08.
SIGNORiQ6SA08.

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 4 (EC:2.7.11.1)
Short name:
TSK-4
Short name:
TSSK-4
Short name:
Testis-specific kinase 4
Alternative name(s):
Serine/threonine-protein kinase 22E
Gene namesi
Name:TSSK4
Synonyms:C14orf20, STK22E, TSSK5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:19825. TSSK4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi283629.
OpenTargetsiENSG00000139908.
PharmGKBiPA134908318.

Polymorphism and mutation databases

BioMutaiTSSK4.
DMDMi62287888.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867721 – 328Testis-specific serine/threonine-protein kinase 4Add BLAST328

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6SA08.
PeptideAtlasiQ6SA08.
PRIDEiQ6SA08.

PTM databases

iPTMnetiQ6SA08.
PhosphoSitePlusiQ6SA08.

Expressioni

Tissue specificityi

Expressed only in the testis.1 Publication

Gene expression databases

BgeeiENSG00000139908.
CleanExiHS_TSSK4.
ExpressionAtlasiQ6SA08. baseline and differential.
GenevisibleiQ6SA08. HS.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CREB1P162205EBI-1202583,EBI-711855

Protein-protein interaction databases

BioGridi129625. 1 interactor.
IntActiQ6SA08. 1 interactor.
STRINGi9606.ENSP00000339179.

Structurei

3D structure databases

ProteinModelPortaliQ6SA08.
SMRiQ6SA08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ6SA08.
KOiK08811.
OMAiDYCHERG.
OrthoDBiEOG091G0EZI.
PhylomeDBiQ6SA08.
TreeFamiTF105333.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6SA08-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKGDVLEAA PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM
60 70 80 90 100
VAVKIISKKK ASDDYLNKFL PREIQVMKVL RHKYLINFYR AIESTSRVYI
110 120 130 140 150
ILELAQGGDV LEWIQRYGAC SEPLAGKWFS QLTLGIAYLH SKSIVHRDLK
160 170 180 190 200
LENLLLDKWE NVKISDFGFA KMVPSNQPVG CSPSYRQVNC FSHLSQTYCG
210 220 230 240 250
SFAYACPEIL RGLPYNPFLS DTWSMGVILY TLVVAHLPFD DTNLKKLLRE
260 270 280 290 300
TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD SWVLKFQPEQ
310 320
PTHEIRLLEA MCQLHNTTKQ HQSLQITT
Length:328
Mass (Da):37,454
Last modified:July 5, 2004 - v1
Checksum:iC78502FF6F71754C
GO
Isoform 2 (identifier: Q6SA08-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: R → RLMPSLSAAGR

Show »
Length:338
Mass (Da):38,439
Checksum:i8E3CC56DBF1B5F78
GO
Isoform 3 (identifier: Q6SA08-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: No experimental confirmation available.
Show »
Length:252
Mass (Da):29,034
Checksum:i0142331DA68E06C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115Q → R in BAC85482 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04124733H → Y.1 PublicationCorresponds to variant rs36036137dbSNPEnsembl.1
Natural variantiVAR_04124889Y → C.1 PublicationCorresponds to variant rs34083933dbSNPEnsembl.1
Natural variantiVAR_041249145V → M.1 PublicationCorresponds to variant rs35468205dbSNPEnsembl.1
Natural variantiVAR_041250196Q → R.1 PublicationCorresponds to variant rs1270764dbSNPEnsembl.1
Natural variantiVAR_041251327T → M.1 PublicationCorresponds to variant rs35244223dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0235591 – 76Missing in isoform 3. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_023560147R → RLMPSLSAAGR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461663 mRNA. Translation: AAS17971.1.
AK131042 mRNA. Translation: BAC85482.1.
BC111088 mRNA. Translation: AAI11089.1.
CCDSiCCDS53890.1. [Q6SA08-2]
CCDS76662.1. [Q6SA08-3]
CCDS9618.1. [Q6SA08-1]
RefSeqiNP_001171668.1. NM_001184739.1. [Q6SA08-2]
NP_001294996.1. NM_001308067.1. [Q6SA08-3]
NP_777604.2. NM_174944.3. [Q6SA08-1]
UniGeneiHs.314432.

Genome annotation databases

EnsembliENST00000287913; ENSP00000287913; ENSG00000139908. [Q6SA08-1]
ENST00000339917; ENSP00000339179; ENSG00000139908. [Q6SA08-2]
ENST00000556621; ENSP00000452054; ENSG00000139908. [Q6SA08-3]
GeneIDi283629.
KEGGihsa:283629.
UCSCiuc001wne.4. human. [Q6SA08-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461663 mRNA. Translation: AAS17971.1.
AK131042 mRNA. Translation: BAC85482.1.
BC111088 mRNA. Translation: AAI11089.1.
CCDSiCCDS53890.1. [Q6SA08-2]
CCDS76662.1. [Q6SA08-3]
CCDS9618.1. [Q6SA08-1]
RefSeqiNP_001171668.1. NM_001184739.1. [Q6SA08-2]
NP_001294996.1. NM_001308067.1. [Q6SA08-3]
NP_777604.2. NM_174944.3. [Q6SA08-1]
UniGeneiHs.314432.

3D structure databases

ProteinModelPortaliQ6SA08.
SMRiQ6SA08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129625. 1 interactor.
IntActiQ6SA08. 1 interactor.
STRINGi9606.ENSP00000339179.

PTM databases

iPTMnetiQ6SA08.
PhosphoSitePlusiQ6SA08.

Polymorphism and mutation databases

BioMutaiTSSK4.
DMDMi62287888.

Proteomic databases

PaxDbiQ6SA08.
PeptideAtlasiQ6SA08.
PRIDEiQ6SA08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000287913; ENSP00000287913; ENSG00000139908. [Q6SA08-1]
ENST00000339917; ENSP00000339179; ENSG00000139908. [Q6SA08-2]
ENST00000556621; ENSP00000452054; ENSG00000139908. [Q6SA08-3]
GeneIDi283629.
KEGGihsa:283629.
UCSCiuc001wne.4. human. [Q6SA08-1]

Organism-specific databases

CTDi283629.
DisGeNETi283629.
GeneCardsiTSSK4.
HGNCiHGNC:19825. TSSK4.
MIMi610711. gene.
neXtProtiNX_Q6SA08.
OpenTargetsiENSG00000139908.
PharmGKBiPA134908318.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ6SA08.
KOiK08811.
OMAiDYCHERG.
OrthoDBiEOG091G0EZI.
PhylomeDBiQ6SA08.
TreeFamiTF105333.

Enzyme and pathway databases

BioCyciZFISH:HS13803-MONOMER.
BRENDAi2.7.11.1. 2681.
SignaLinkiQ6SA08.
SIGNORiQ6SA08.

Miscellaneous databases

GenomeRNAii283629.
PROiQ6SA08.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000139908.
CleanExiHS_TSSK4.
ExpressionAtlasiQ6SA08. baseline and differential.
GenevisibleiQ6SA08. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSSK4_HUMAN
AccessioniPrimary (citable) accession number: Q6SA08
Secondary accession number(s): Q2TA60, Q6ZNM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.