Reviewed, UniProtKB/Swiss-Prot Q6SA08 (TSSK4_HUMAN)

Names and origin

Protein names

Recommended name:
    Testis-specific serine/threonine-protein kinase 4
      Short name=TSSK-4
      Short name=Testis-specific kinase 4
      Short name=TSK-4
    EC=2.7.11.1
Alternative name(s):
    Serine/threonine-protein kinase 22E

Gene names

Name:	TSSK4
Synonyms:	C14orf20, STK22E, TSSK5

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in a signaling pathway during male germ cell development or mature sperm function By similarity. Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Enzyme regulation	Activated by phosphorylation on Thr-197, potentially by autophosphorylation By similarity.
Tissue specificity	Expressed only in the testis. Ref.1
Post-translational modification	Autophosphorylated By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of CREB transcription factor activity Ref.1 Inferred from direct assay. Source: UniProtKB protein amino acid phosphorylation Ref.1 Inferred from direct assay. Source: UniProtKB spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Ref.1 Inferred from direct assay. Source: UniProtKB magnesium ion binding Ref.1 Inferred from direct assay. Source: UniProtKB protein binding Ref.1 Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CREB1	P16220	4	EBI-1202583,EBI-711855

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6SA08-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6SA08-2)

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: R → RLMPSLSAAGR

Isoform 3 (identifier: Q6SA08-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Testis-specific serine/threonine-protein kinase 4		PRO_0000086772
Regions
	Domain	25 – 293	269	Protein kinase
	Nucleotide binding	31 – 39	9	ATP By similarity
Sites
	Active site	148	1	Proton acceptor By similarity
	Binding site	54	1	ATP By similarity
Amino acid modifications
	Modified residue	197	1	Phosphothreonine By similarity
	Modified residue	251	1	Phosphothreonine Ref.4
	Modified residue	256	1	Phosphothreonine Ref.4
	Modified residue	264	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	1 – 76	76	Missing in isoform 3.		VSP_023559
	Alternative sequence	147	1	R → RLMPSLSAAGR in isoform 2.		VSP_023560
	Natural variant	33	1	H → Y: dbSNP rs36036137. Ref.5		VAR_041247
	Natural variant	89	1	Y → C: dbSNP rs34083933. Ref.5		VAR_041248
	Natural variant	145	1	V → M		VAR_041249
	Natural variant	196	1	Q → R: dbSNP rs1270764. Ref.5		VAR_041250
	Natural variant	327	1	T → M		VAR_041251
Experimental info
	Sequence conflict	115	1	Q → R in BAC85482. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C78502FF6F71754C	FASTA	328	37,454
	10 20 30 40 50 60 MGKGDVLEAA PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM VAVKIISKKK 70 80 90 100 110 120 ASDDYLNKFL PREIQVMKVL RHKYLINFYR AIESTSRVYI ILELAQGGDV LEWIQRYGAC 130 140 150 160 170 180 SEPLAGKWFS QLTLGIAYLH SKSIVHRDLK LENLLLDKWE NVKISDFGFA KMVPSNQPVG 190 200 210 220 230 240 CSPSYRQVNC FSHLSQTYCG SFAYACPEIL RGLPYNPFLS DTWSMGVILY TLVVAHLPFD 250 260 270 280 290 300 DTNLKKLLRE TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD SWVLKFQPEQ 310 320 PTHEIRLLEA MCQLHNTTKQ HQSLQITT
	Isoform 2. Checksum: 8E3CC56DBF1B5F78	FASTA	338	38,439
	10 20 30 40 50 60 MGKGDVLEAA PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM VAVKIISKKK 70 80 90 100 110 120 ASDDYLNKFL PREIQVMKVL RHKYLINFYR AIESTSRVYI ILELAQGGDV LEWIQRYGAC 130 140 150 160 170 180 SEPLAGKWFS QLTLGIAYLH SKSIVHRLMP SLSAAGRDLK LENLLLDKWE NVKISDFGFA 190 200 210 220 230 240 KMVPSNQPVG CSPSYRQVNC FSHLSQTYCG SFAYACPEIL RGLPYNPFLS DTWSMGVILY 250 260 270 280 290 300 TLVVAHLPFD DTNLKKLLRE TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD 310 320 330 SWVLKFQPEQ PTHEIRLLEA MCQLHNTTKQ HQSLQITT
	Isoform 3. Checksum: 0142331DA68E06C9	FASTA	252	29,034
	10 20 30 40 50 60 MKVLRHKYLI NFYRAIESTS RVYIILELAQ GGDVLEWIQR YGACSEPLAG KWFSQLTLGI 70 80 90 100 110 120 AYLHSKSIVH RDLKLENLLL DKWENVKISD FGFAKMVPSN QPVGCSPSYR QVNCFSHLSQ 130 140 150 160 170 180 TYCGSFAYAC PEILRGLPYN PFLSDTWSMG VILYTLVVAH LPFDDTNLKK LLRETQKEVT 190 200 210 220 230 240 FPANHTISQE CKNLILQMLR QATKRATILD IIKDSWVLKF QPEQPTHEIR LLEAMCQLHN 250 TTKQHQSLQI TT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway." Chen X., Lin G., Wei Y., Hexige S., Niu Y., Liu L., Yang C., Yu L. Biochem. Biophys. Res. Commun. 333:742-749(2005) [PubMed: 15964553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-251; THR-256 AND SER-264, MASS SPECTROMETRY.
[5]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] TYR-33; CYS-89; MET-145; ARG-196 AND MET-327.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY461663 mRNA. Translation: AAS17971.1. AK131042 mRNA. Translation: BAC85482.1. BC111088 mRNA. Translation: AAI11089.1.
IPI	IPI00414440. IPI00646936. IPI00829727.
RefSeq	NP_777604.2.
UniGene	Hs.314432
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6SA08. 1 interaction.
STRING	Q6SA08.
PTM databases
PhosphoSite	Q6SA08.
Proteomic databases
PRIDE	Q6SA08.
Genome annotation databases
Ensembl	ENST00000287913; ENSP00000287913; ENSG00000139908; Homo sapiens. [Genome view] ENST00000339917; ENSP00000339179; ENSG00000139908; Homo sapiens. [Genome view] ENST00000424073; ENSP00000390516; ENSG00000139908; Homo sapiens. [Genome view] ENST00000428351; ENSP00000412180; ENSG00000139908; Homo sapiens. [Genome view]
GeneID	283629.
KEGG	hsa:283629.
UCSC	uc001wne.1. human. uc001wng.1. human. uc001wnh.1. human.
Organism-specific databases
CTD	283629.
GeneCards	GC14P023745.
HGNC	HGNC:19825. TSSK4.
MIM	610711. gene.
PharmGKB	PA134908318.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q6SA08.
OMA	VAHLPFD.
Enzyme and pathway databases
BRENDA	2.7.11.1. 247.
Gene expression databases
ArrayExpress	Q6SA08.
Bgee	Q6SA08.
CleanEx	HS_TSSK4.
Genevestigator	Q6SA08.
GermOnline	ENSG00000139908. Homo sapiens.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. IPR015737. Testis-specific_ser-thr_kinase. [Graphical view]
PANTHER	PTHR22982:SF46. Testis_ser_thr_kin. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	94101.
SOURCE	Search...

Entry information

Entry name

TSSK4_HUMAN

Accession

Primary (citable) accession number: Q6SA08
Secondary accession number(s): Q2TA60, Q6ZNM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.