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Q6SA08

- TSSK4_HUMAN

UniProt

Q6SA08 - TSSK4_HUMAN

Protein

Testis-specific serine/threonine-protein kinase 4

Gene

TSSK4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    May be involved in a signaling pathway during male germ cell development or mature sperm function By similarity. Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.By similarity1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Activated by phosphorylation on Thr-197, potentially by autophosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei148 – 1481Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. positive regulation of CREB transcription factor activity Source: UniProtKB
    4. protein phosphorylation Source: UniProtKB
    5. spermatogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 2681.
    SignaLinkiQ6SA08.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Testis-specific serine/threonine-protein kinase 4 (EC:2.7.11.1)
    Short name:
    TSK-4
    Short name:
    TSSK-4
    Short name:
    Testis-specific kinase 4
    Alternative name(s):
    Serine/threonine-protein kinase 22E
    Gene namesi
    Name:TSSK4
    Synonyms:C14orf20, STK22E, TSSK5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:19825. TSSK4.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134908318.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Testis-specific serine/threonine-protein kinase 4PRO_0000086772Add
    BLAST

    Post-translational modificationi

    Autophosphorylated.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ6SA08.
    PRIDEiQ6SA08.

    PTM databases

    PhosphoSiteiQ6SA08.

    Expressioni

    Tissue specificityi

    Expressed only in the testis.1 Publication

    Gene expression databases

    ArrayExpressiQ6SA08.
    BgeeiQ6SA08.
    CleanExiHS_TSSK4.
    GenevestigatoriQ6SA08.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CREB1P162205EBI-1202583,EBI-711855

    Protein-protein interaction databases

    BioGridi129625. 1 interaction.
    IntActiQ6SA08. 1 interaction.
    STRINGi9606.ENSP00000287913.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6SA08.
    SMRiQ6SA08. Positions 18-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 293269Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233016.
    HOVERGENiHBG101110.
    KOiK08811.
    OMAiIIKDPWV.
    OrthoDBiEOG7P8P8F.
    PhylomeDBiQ6SA08.
    TreeFamiTF105333.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6SA08-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGKGDVLEAA PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM    50
    VAVKIISKKK ASDDYLNKFL PREIQVMKVL RHKYLINFYR AIESTSRVYI 100
    ILELAQGGDV LEWIQRYGAC SEPLAGKWFS QLTLGIAYLH SKSIVHRDLK 150
    LENLLLDKWE NVKISDFGFA KMVPSNQPVG CSPSYRQVNC FSHLSQTYCG 200
    SFAYACPEIL RGLPYNPFLS DTWSMGVILY TLVVAHLPFD DTNLKKLLRE 250
    TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD SWVLKFQPEQ 300
    PTHEIRLLEA MCQLHNTTKQ HQSLQITT 328
    Length:328
    Mass (Da):37,454
    Last modified:July 5, 2004 - v1
    Checksum:iC78502FF6F71754C
    GO
    Isoform 2 (identifier: Q6SA08-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         147-147: R → RLMPSLSAAGR

    Show »
    Length:338
    Mass (Da):38,439
    Checksum:i8E3CC56DBF1B5F78
    GO
    Isoform 3 (identifier: Q6SA08-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-76: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:252
    Mass (Da):29,034
    Checksum:i0142331DA68E06C9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti115 – 1151Q → R in BAC85482. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti33 – 331H → Y.1 Publication
    Corresponds to variant rs36036137 [ dbSNP | Ensembl ].
    VAR_041247
    Natural varianti89 – 891Y → C.1 Publication
    Corresponds to variant rs34083933 [ dbSNP | Ensembl ].
    VAR_041248
    Natural varianti145 – 1451V → M.1 Publication
    Corresponds to variant rs35468205 [ dbSNP | Ensembl ].
    VAR_041249
    Natural varianti196 – 1961Q → R.1 Publication
    Corresponds to variant rs1270764 [ dbSNP | Ensembl ].
    VAR_041250
    Natural varianti327 – 3271T → M.1 Publication
    Corresponds to variant rs35244223 [ dbSNP | Ensembl ].
    VAR_041251

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7676Missing in isoform 3. 1 PublicationVSP_023559Add
    BLAST
    Alternative sequencei147 – 1471R → RLMPSLSAAGR in isoform 2. 1 PublicationVSP_023560

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY461663 mRNA. Translation: AAS17971.1.
    AK131042 mRNA. Translation: BAC85482.1.
    BC111088 mRNA. Translation: AAI11089.1.
    CCDSiCCDS53890.1. [Q6SA08-2]
    CCDS9618.1. [Q6SA08-1]
    RefSeqiNP_001171668.1. NM_001184739.1. [Q6SA08-2]
    NP_777604.2. NM_174944.3. [Q6SA08-1]
    UniGeneiHs.314432.

    Genome annotation databases

    EnsembliENST00000287913; ENSP00000287913; ENSG00000139908. [Q6SA08-1]
    ENST00000339917; ENSP00000339179; ENSG00000139908. [Q6SA08-2]
    ENST00000556621; ENSP00000452054; ENSG00000139908. [Q6SA08-3]
    GeneIDi283629.
    KEGGihsa:283629.
    UCSCiuc001wne.3. human. [Q6SA08-1]
    uc001wnh.3. human. [Q6SA08-2]

    Polymorphism databases

    DMDMi62287888.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY461663 mRNA. Translation: AAS17971.1 .
    AK131042 mRNA. Translation: BAC85482.1 .
    BC111088 mRNA. Translation: AAI11089.1 .
    CCDSi CCDS53890.1. [Q6SA08-2 ]
    CCDS9618.1. [Q6SA08-1 ]
    RefSeqi NP_001171668.1. NM_001184739.1. [Q6SA08-2 ]
    NP_777604.2. NM_174944.3. [Q6SA08-1 ]
    UniGenei Hs.314432.

    3D structure databases

    ProteinModelPortali Q6SA08.
    SMRi Q6SA08. Positions 18-321.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 129625. 1 interaction.
    IntActi Q6SA08. 1 interaction.
    STRINGi 9606.ENSP00000287913.

    PTM databases

    PhosphoSitei Q6SA08.

    Polymorphism databases

    DMDMi 62287888.

    Proteomic databases

    PaxDbi Q6SA08.
    PRIDEi Q6SA08.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000287913 ; ENSP00000287913 ; ENSG00000139908 . [Q6SA08-1 ]
    ENST00000339917 ; ENSP00000339179 ; ENSG00000139908 . [Q6SA08-2 ]
    ENST00000556621 ; ENSP00000452054 ; ENSG00000139908 . [Q6SA08-3 ]
    GeneIDi 283629.
    KEGGi hsa:283629.
    UCSCi uc001wne.3. human. [Q6SA08-1 ]
    uc001wnh.3. human. [Q6SA08-2 ]

    Organism-specific databases

    CTDi 283629.
    GeneCardsi GC14P024674.
    HGNCi HGNC:19825. TSSK4.
    MIMi 610711. gene.
    neXtProti NX_Q6SA08.
    PharmGKBi PA134908318.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233016.
    HOVERGENi HBG101110.
    KOi K08811.
    OMAi IIKDPWV.
    OrthoDBi EOG7P8P8F.
    PhylomeDBi Q6SA08.
    TreeFami TF105333.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 2681.
    SignaLinki Q6SA08.

    Miscellaneous databases

    GenomeRNAii 283629.
    NextBioi 94101.
    PROi Q6SA08.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6SA08.
    Bgeei Q6SA08.
    CleanExi HS_TSSK4.
    Genevestigatori Q6SA08.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway."
      Chen X., Lin G., Wei Y., Hexige S., Niu Y., Liu L., Yang C., Yu L.
      Biochem. Biophys. Res. Commun. 333:742-749(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Testis.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    4. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] TYR-33; CYS-89; MET-145; ARG-196 AND MET-327.

    Entry informationi

    Entry nameiTSSK4_HUMAN
    AccessioniPrimary (citable) accession number: Q6SA08
    Secondary accession number(s): Q2TA60, Q6ZNM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3