Q6S9C8 (LKHA4_CHILA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leukotriene A-4 hydrolase
Short name=LTA-4 hydrolase
EC=3.3.2.6

Alternative name(s):
Leukotriene A(4) hydrolase

Gene names

Name:

LTA4H

Organism

Chinchilla lanigera (Long-tailed chinchilla) (Chinchilla villidera)

Taxonomic identifier

34839 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Chinchillidae › Chinchilla

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity By similarity.
Catalytic activity	(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Enzyme regulation	Inhibited by bestatin. Subject to suicide inhibition by leukotriene A4 By similarity.
Pathway	Lipid metabolism; leukotriene B4 biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Acetylation
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB peptide catabolic process Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB epoxide hydrolase activity Inferred from sequence or structural similarity. Source: UniProtKB leukotriene-A4 hydrolase activity Inferred from sequence or structural similarity. Source: UniProtKB metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 611	610	Leukotriene A-4 hydrolase		PRO_0000095123
Regions
	Region	135 – 137	3	Substrate binding By similarity
	Region	267 – 272	6	Substrate binding By similarity
	Region	564 – 566	3	Substrate binding By similarity
Sites
	Active site	297	1	Proton acceptor By similarity
	Active site	384	1	Proton donor By similarity
	Metal binding	296	1	Zinc; catalytic By similarity
	Metal binding	300	1	Zinc; catalytic By similarity
	Metal binding	319	1	Zinc; catalytic By similarity
	Site	376	1	Essential for epoxide hydrolase activity, but not for aminopeptidase activity By similarity
	Site	379	1	Covalently modified during suicide inhibition by leukotrienes By similarity
Amino acid modifications
	Modified residue	73	1	N6-acetyllysine By similarity
	Modified residue	337	1	N6-acetyllysine By similarity
	Modified residue	573	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6S9C8 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3E358988E8B98044	FASTA	611	69,213
	10 20 30 40 50 60 MPEVEDTCFL TSPITVCRTK HLHLRCSVDF SSRALTGIAA LTIQSQEDNL RSLVLDTKAL 70 80 90 100 110 120 TIEKVVINGQ EVKYALGERQ SYKGSPMEIS LPIALSKNQE VVIEISFETS PKSSALQWLT 130 140 150 160 170 180 PEQTSGKEHP YLFSQCQAIH CRAVLPCQDT PSVKLTYTAE VSVPKELVAL MSAVRDGETP 190 200 210 220 230 240 DPEDPSRKIY KFNQKVPIPC YLIALVVGAL ESRKIGPRTL VWSEKEQVEK SAYEFSETES 250 260 270 280 290 300 MLKIAEDLGG PYVWGQYDLL VLPPSFPYGG MENPCLTFVT PTLLAGDKSL SNVIAHEISH 310 320 330 340 350 360 SWTGNLVTNK TWDHFWLNEG HTVYLERHIC GRLFGEKFRH FHALGGWGEL QNTIKTFGET 370 380 390 400 410 420 HPFTKLVVDL TDVDPDVAYS SVPYEKGFAL LFHLEQLLGG PEVFLGFLKA YVERFSYKSI 430 440 450 460 470 480 TTDDWKNFLY SHFKDKVDIL NQVDWNAWLY SPGLPPVKPN YDMTLTNACI ALSQRWITAK 490 500 510 520 530 540 EEDLNTFNAT DLKDLSTHQV NEFLAQVLQQ APLPLGHVKR MQEVYNFNAV NNSEIRFRWL 550 560 570 580 590 600 RLCIQSKWEE AIPLALKMAT EQGRMKFTRP LFKDLAAFDK SHDQAIRTYQ AHKASMHPVT 610 AMLVGKDLKV D

References

[1]	"The chinchilla leukotriene A4 hydrolase gene is expressed in normal middle ear mucosa." Erdos G., Hu F.Z., Donfack J., Ahmed A.I., Preston R.A., Post J.C., Ehrlich G.D. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Middle ear mucosa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY462137 mRNA. Translation: AAR26536.1.
3D structure databases
ProteinModelPortal	Q6S9C8.
SMR	Q6S9C8. Positions 2-611.
ModBase	Search...
Protein family/group databases
MEROPS	M01.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG001274.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR012777. Leukotriene_A4_hydrolase. IPR001930. Peptidase_M1. IPR015211. Peptidase_M1_C. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF09127. Leuk-A4-hydro_C. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
TIGRFAMs	TIGR02411. Leuko_A4_hydro. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LKHA4_CHILA

Accession

Primary (citable) accession number: Q6S9C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 48 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program