Q6S8J3 (POTEE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
POTE ankyrin domain family member E

Alternative name(s):
ANKRD26-like family C member 1A
Prostate, ovary, testis-expressed protein on chromosome 2
Short name=POTE-2

Gene names

Name:	POTEE
Synonyms:	A26C1A, POTE2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1075 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

In the N-terminal section; belongs to the POTE family. In the C-terminal section; belongs to the actin family. Contains 5 ANK repeats.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Coiled coil Repeat
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6S8J3-1)

Also known as: POTE2A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6S8J3-2)

Also known as: POTE2B;

The sequence of this isoform differs from the canonical sequence as follows:
     212-212: K → KVCSSQLYQHE
     377-384: QELKLTSE → NVSRTRNK
     385-1075: Missing.

Isoform 3 (identifier: Q6S8J3-3)

Also known as: POTE2C; POTE2D;

The sequence of this isoform differs from the canonical sequence as follows:
     376-376: E → G
     377-1075: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1075	1075	POTE ankyrin domain family member E		PRO_0000066914
Regions
	Repeat	172 – 201	30	ANK 1
	Repeat	205 – 234	30	ANK 2
	Repeat	238 – 267	30	ANK 3
	Repeat	271 – 300	30	ANK 4
	Repeat	304 – 333	30	ANK 5
	Region	702 – 1075	374	Actin-like
	Coiled coil	399 – 435	37	Potential
	Coiled coil	642 – 698	57	Potential
Amino acid modifications
	Modified residue	866	1	Phosphotyrosine Ref.4
	Modified residue	875	1	Phosphothreonine Ref.4
	Cross-link	684		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
Natural variations
	Alternative sequence	212	1	K → KVCSSQLYQHE in isoform 2.		VSP_011969
	Alternative sequence	376	1	E → G in isoform 3.		VSP_011970
	Alternative sequence	377 – 1075	699	Missing in isoform 3.		VSP_011971
	Alternative sequence	377 – 384	8	QELKLTSE → NVSRTRNK in isoform 2.		VSP_011972
	Alternative sequence	385 – 1075	691	Missing in isoform 2.		VSP_011973
Experimental info
	Sequence conflict	346	1	V → G in AAS58867. Ref.2
	Sequence conflict	378	1	E → D in AAS58865. Ref.2
	Sequence conflict	546	1	G → R in AAS58865. Ref.2
	Sequence conflict	563	1	G → A in AAS58865. Ref.2
	Sequence conflict	578	1	P → L in AAS58865. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (POTE2A) [UniParc]. Last modified February 5, 2008. Version 3. Checksum: 581F842CDDC13EFC	FASTA	1,075	121,363
	10 20 30 40 50 60 MVVEVDSMPA ASSVKKPFGL RSKMGKWCCR CFPCYRESGK SNVGTSGDHD DSAMKTLRSK 70 80 90 100 110 120 MGKWCHHCFP CCRGSGKSNV GASGDHDDSA MKTLRNKMGK WCCHCFPCCR GSGKSKVGAW 130 140 150 160 170 180 GDYDDSAFME PRYHVRGEDL DKLHRAAWWG KVPRKDLIVM LRDTDVNKKD KQKRTALHLA 190 200 210 220 230 240 SANGNSEVVK LLLDRRCQLN VLDNKKRTAL IKAVQCQEDE CALMLLEHGT DPNIPDEYGN 250 260 270 280 290 300 TTLHYAIYNE DKLMAKALLL YGADIESKNK HGLTPLLLGV HEQKQQVVKF LIKKKANLNA 310 320 330 340 350 360 LDRYGRTALI LAVCCGSASI VSLLLEQNID VSSQDLSGQT AREYAVSSHH HVICQLLSDY 370 380 390 400 410 420 KEKQMLKISS ENSNPEQELK LTSEEESQRF KGSENSQPEK MSQELEINKD GDREVEEEMK 430 440 450 460 470 480 KHESNNVGLL ENLTNGVTAG NGDNGLIPQR KSRTPENQQF PDNESEEYHR ICELLSDYKE 490 500 510 520 530 540 KQMPKYSSEN SNPEQDLKLT SEEESQRLKG SENGQPEKRS QEPEINKDGD RELENFMAIE 550 560 570 580 590 600 EMKKHGSTHV GFPENLTNGA TAGNGDDGLI PPRKSRTPES QQFPDTENEE YHSDEQNDTQ 610 620 630 640 650 660 KQFCEEQNTG ILHDEILIHE EKQIEVVEKM NSELSLSCKK EKDVLHENST LREEIAMLRL 670 680 690 700 710 720 ELDTMKHQSQ LREKKYLEDI ESVKKKNDNL LKALQLNELT MDDDTAVLVI DNGSGMCKAG 730 740 750 760 770 780 FAGDDAPRAV FPSIVGRPRQ QGMMGGMHQK ESYVGKEAQS KRGILTLKYP MEHGIITNWD 790 800 810 820 830 840 DMEKIWHHTF YNELRVAPEE HPILLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVP 850 860 870 880 890 900 SLYTSGRTTG IVMDSGDGVT HTVPIYEGNA LPHATLRLDL AGRELPDYLM KILTERGYRF 910 920 930 940 950 960 TTMAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA 970 980 990 1000 1010 1020 LFQPCFLGME SCGIHETTFN SIMKSDVDIR KDLYTNTVLS GGTTMYPGMA HRMQKEIAAL 1030 1040 1050 1060 1070 APSMMKIRII APPKRKYSVW VGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF
	Isoform 2 (POTE2B) [UniParc]. Checksum: 58C1E27852BD4DA4	FASTA	394	44,113
	10 20 30 40 50 60 MVVEVDSMPA ASSVKKPFGL RSKMGKWCCR CFPCYRESGK SNVGTSGDHD DSAMKTLRSK 70 80 90 100 110 120 MGKWCHHCFP CCRGSGKSNV GASGDHDDSA MKTLRNKMGK WCCHCFPCCR GSGKSKVGAW 130 140 150 160 170 180 GDYDDSAFME PRYHVRGEDL DKLHRAAWWG KVPRKDLIVM LRDTDVNKKD KQKRTALHLA 190 200 210 220 230 240 SANGNSEVVK LLLDRRCQLN VLDNKKRTAL IKVCSSQLYQ HEAVQCQEDE CALMLLEHGT 250 260 270 280 290 300 DPNIPDEYGN TTLHYAIYNE DKLMAKALLL YGADIESKNK HGLTPLLLGV HEQKQQVVKF 310 320 330 340 350 360 LIKKKANLNA LDRYGRTALI LAVCCGSASI VSLLLEQNID VSSQDLSGQT AREYAVSSHH 370 380 390 HVICQLLSDY KEKQMLKISS ENSNPENVSR TRNK
	Isoform 3 (POTE2C) (POTE2D) [UniParc]. Checksum: 7804E08803E5A577	FASTA	376	41,909
	10 20 30 40 50 60 MVVEVDSMPA ASSVKKPFGL RSKMGKWCCR CFPCYRESGK SNVGTSGDHD DSAMKTLRSK 70 80 90 100 110 120 MGKWCHHCFP CCRGSGKSNV GASGDHDDSA MKTLRNKMGK WCCHCFPCCR GSGKSKVGAW 130 140 150 160 170 180 GDYDDSAFME PRYHVRGEDL DKLHRAAWWG KVPRKDLIVM LRDTDVNKKD KQKRTALHLA 190 200 210 220 230 240 SANGNSEVVK LLLDRRCQLN VLDNKKRTAL IKAVQCQEDE CALMLLEHGT DPNIPDEYGN 250 260 270 280 290 300 TTLHYAIYNE DKLMAKALLL YGADIESKNK HGLTPLLLGV HEQKQQVVKF LIKKKANLNA 310 320 330 340 350 360 LDRYGRTALI LAVCCGSASI VSLLLEQNID VSSQDLSGQT AREYAVSSHH HVICQLLSDY 370 KEKQMLKISS ENSNPG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Five POTE paralogs and their splice variants are expressed in human prostate and encode proteins of different lengths." Bera T.K., Huynh N., Maeda H., Sathyanarayana B.K., Lee B., Pastan I. Gene 337:45-53(2004) [PubMed: 15276201] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-712 (ISOFORMS 1; 2 AND 3). Tissue: Prostate.
[3]	"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry." Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D. Proteomics 7:868-874(2007) [PubMed: 17370265] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-684, MASS SPECTROMETRY. Tissue: Mammary cancer.
[4]	"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment." Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J. J. Proteome Res. 7:5167-5176(2008) [PubMed: 19367720] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-866 AND THR-875, MASS SPECTROMETRY. Tissue: T-cell.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC131180 Genomic DNA. No translation available. AY462868 mRNA. Translation: AAS58862.1. AY462871 mRNA. Translation: AAS58865.1. AY462872 mRNA. Translation: AAS58866.1. AY462873 mRNA. Translation: AAS58867.1.
IPI	IPI00470363. IPI00479743. IPI00867651.
RefSeq	NP_001077007.1. NM_001083538.1.
UniGene	Hs.686146.
3D structure databases
ProteinModelPortal	Q6S8J3.
SMR	Q6S8J3. Positions 137-364, 702-1075.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6S8J3. 1 interaction.
STRING	Q6S8J3.
PTM databases
PhosphoSite	Q6S8J3.
Polymorphism databases
DMDM	166898073.
Proteomic databases
PRIDE	Q6S8J3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000420985; ENSP00000421734; ENSG00000250852.
GeneID	445582.
KEGG	hsa:445582.
UCSC	uc002tsn.2. human.
Organism-specific databases
CTD	445582.
GeneCards	GC02P131980. GC02P131981.
H-InvDB	HIX0029907.
HGNC	HGNC:33895. POTEE.
MIM	608914. gene.
neXtProt	NX_Q6S8J3.
PharmGKB	PA164724783.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17475.
GeneTree	ENSGT00560000076605.
HOGENOM	HBG506345.
InParanoid	Q6S8J3.
OrthoDB	EOG4SN1NB.
PhylomeDB	Q6S8J3.
Gene expression databases
Bgee	Q6S8J3.
CleanEx	HS_POTEE.
Genevestigator	Q6S8J3.
GermOnline	ENSG00000188219. Homo sapiens.
Family and domain databases
InterPro	IPR004000. Actin-like. IPR020902. Actin/actin-like_CS. IPR004001. Actin_CS. IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00022. Actin. 1 hit. PF00023. Ank. 2 hits. PF12796. Ank_2. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. SM00248. ANK. 6 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS00432. ACTINS_2. 1 hit. PS01132. ACTINS_ACT_LIKE. 1 hit. PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	111402.
SOURCE	Search...

Entry information

Entry name

POTEE_HUMAN

Accession

Primary (citable) accession number: Q6S8J3
Secondary accession number(s): Q6S8J4, Q6S8J5, Q6S8J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.