Q6S7F2 (E2F7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor E2F7
Short name=E2F-7

Gene names

Name:

E2f7

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	904 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Along with E2F8, inhibitor of E2F-dependent transcription that is important for embryonic development and for the control of the E2f1-Tp53 apoptotic pathway. Directly represses E2F1 transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. Ref.1 Ref.3
Subunit structure	Forms homodimers and, to a lesser extent, heterodimers with E2F8. Dimerization is important for DNA binding By similarity.
Subcellular location	Nucleus Ref.1.
Tissue specificity	Widely expressed with highest levels in skin and thymus and very low levels in brain, muscle and stomach. Ref.1
Domain	Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer By similarity.
Disruption phenotype	Mice develop normally and live to old age. E2f7/E2f8 double-knockout embryos die by 11.5 dpc of massive apoptosis and dilation of blood vessels and show increased expression of E2f1 and Tp53, as well as many stress-related genes. Ref.3
Sequence similarities	Belongs to the E2F/DP family.
Sequence caution	The sequence BAC32193.1 differs from that shown. Reason: Frameshift at position 738.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell proliferation Inferred from direct assay Ref.1. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor complex Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 904	904	Transcription factor E2F7		PRO_0000298908
Regions
	DNA binding	143 – 212	70	Potential
	DNA binding	283 – 368	86	Potential
Amino acid modifications
	Modified residue	96	1	Phosphoserine By similarity
	Modified residue	411	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	73	1	V → D in BAC27243. Ref.2
	Sequence conflict	159	1	Y → H in BAC32193. Ref.2
	Sequence conflict	300	1	L → H in BAC27243. Ref.2
	Sequence conflict	776	1	N → D in BAC32193. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6S7F2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1EABCD1805FF80D2	FASTA	904	99,535
	10 20 30 40 50 60 MEVNCLTLKD LISPRQTRLD FAIEDAENAQ KENIFVDRSR MTPKTPMKNE PIDLSKQRIF 70 80 90 100 110 120 TPDRNPITPV KPVDRQPQVE PWTPTANLKM LISAASPDIR DREKKKELFR PIENKEDAFV 130 140 150 160 170 180 NSLQLDVAGD GAVDEYEKQR PSRKQKSLGL LCQKFLARYP SYPLSTEKTT ISLDEVAVSL 190 200 210 220 230 240 GVERRRIYDI VNVLESLHLV SRVAKNQYGW HGRHSLPKTL RTLQRLGEEQ KYEEQMACLQ 250 260 270 280 290 300 QKELDLMGYR FGERRKDGSP DPRDPHLLDF SEADYPSSSA NSRKDKSLRI MSQKFVMLFL 310 320 330 340 350 360 VSKTKIVTLD VAAKILIEES QDTPDHSKFK TKVRRLYDIA NVLTSLALIK KVHVTEERGR 370 380 390 400 410 420 KPAFKWIGPV DFSSIDEELL DVSASILPEL KKEAYGQIRV CAKERLVRYG SFNTVHTSEK 430 440 450 460 470 480 IQRKVSSEPS SPQGERQGSA YSLEIGSLAA IYRQKVEDNS QEEAFVSNTA VPPASILDPA 490 500 510 520 530 540 LSMDSEYCVK PLAQPVFSVA QTDLPAFSAQ NGPSGQVGVP VPSAASDTEN LKPALLAGQP 550 560 570 580 590 600 LVYVPSTQLF MLYGSVQEGL SPESRSEEDG GGSDVPADLS VTPSAQKRLC EERDPQEEED 610 620 630 640 650 660 EPAMKRQSQE FEDSPLSLVM PKKPSSSTDL ACPVTMGNGS SPPLEDACVK GQLPAAEEVT 670 680 690 700 710 720 GKAAPNCYVA SECGNPARNP DTEKPSNENE ITKDPSLMQY LYVQSPAGLN GFNMVLPGTQ 730 740 750 760 770 780 TPHTVAPSPA QLPSFGVPCM FLQSPGLGPF PVLYSPAIPG PISSAPGTHP NPGPMNFGLS 790 800 810 820 830 840 TLASASHLLI SPAAMVNPKP STLPCTDPQL RCQPSLNLNP VMPGSHGVIH PESPCYVRHP 850 860 870 880 890 900 VSMVKAEQSP APATPKSIQR RHRETFFKTP GSLGDPVFRR KERNQSRNTS SAQRRLEISS SGPD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of E2F7, a novel mammalian E2F family member capable of blocking cellular proliferation." de Bruin A., Maiti B., Jakoi L., Timmers C., Buerki R., Leone G. J. Biol. Chem. 278:42041-42049(2003) [PubMed: 12893818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo, Forelimb and Spleen.
[3]	"Synergistic function of E2F7 and E2F8 is essential for cell survival and embryonic development." Li J., Ran C., Li E., Gordon F., Comstock G., Siddiqui H., Cleghorn W., Chen H.-Z., Kornacker K., Liu C.-G., Pandit S.K., Khanizadeh M., Weinstein M., Leone G., de Bruin A. Dev. Cell 14:62-75(2008) [PubMed: 18194653] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY463359 mRNA. Translation: AAR26542.1. AK031078 mRNA. Translation: BAC27243.1. AK045040 mRNA. Translation: BAC32193.1. Frameshift. AK143686 mRNA. Translation: BAE25497.1.
IPI	IPI00420139.
RefSeq	NP_848724.2. NM_178609.4.
UniGene	Mm.11747.
3D structure databases
HSSP	HSSP built from PDB template 1CF7 based on UniProtKB Q16254.
ProteinModelPortal	Q6S7F2.
SMR	Q6S7F2. Positions 142-213, 282-366.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6S7F2.
PTM databases
PhosphoSite	Q6S7F2.
Proteomic databases
PRIDE	Q6S7F2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000073781; ENSMUSP00000073453; ENSMUSG00000020185. ENSMUST00000173471; ENSMUSP00000133494; ENSMUSG00000020185.
GeneID	52679.
KEGG	mmu:52679.
UCSC	uc007gzp.1. mouse.
Organism-specific databases
CTD	144455.
MGI	MGI:1289147. E2f7.
Phylogenomic databases
eggNOG	roNOG07017.
GeneTree	ENSGT00530000063616.
HOGENOM	HBG505309.
HOVERGEN	HBG063270.
InParanoid	Q6S7F2.
OMA	QRKVNSE.
OrthoDB	EOG402WRS.
PhylomeDB	Q6S7F2.
Gene expression databases
ArrayExpress	Q6S7F2.
Bgee	Q6S7F2.
CleanEx	MM_E2F7.
Genevestigator	Q6S7F2.
Family and domain databases
InterPro	IPR015633. E2F. IPR003316. E2F_TDP. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 2 hits.
KO	K09391.
PANTHER	PTHR12081. E2F. 1 hit.
Pfam	PF02319. E2F_TDP. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	309327.
SOURCE	Search...

Entry information

Entry name

E2F7_MOUSE

Accession

Primary (citable) accession number: Q6S7F2
Secondary accession number(s): Q8BRE2, Q8BSQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program