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Protein

SHC-transforming protein 4

Gene

Shc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates both Ras-dependent and Ras-independent migratory pathways in melanomas. Contributes to the early phases of agrin-induced tyrosine phosphorylation of CHRNB1.1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: MGI
  • receptor tyrosine kinase binding Source: MGI

GO - Biological processi

  • apoptotic process Source: MGI
  • intracellular signal transduction Source: InterPro
  • positive regulation of cell proliferation Source: MGI
  • regulation of gene expression Source: MGI
  • stem cell differentiation Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
SHC-transforming protein 4
Alternative name(s):
Rai-like protein
Short name:
RaLP
SHC-transforming protein D
Short name:
mShcD
Src homology 2 domain-containing-transforming protein C4
Short name:
SH2 domain protein C4
Gene namesi
Name:Shc4
Synonyms:Shcd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2655364. Shc4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 626626SHC-transforming protein 4PRO_0000337201Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei422 – 4221PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated; the phosphorylation is enhanced by EGF. Phosphorylation at Tyr-422 is required for the interaction with GRB2 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6S5L9.
PRIDEiQ6S5L9.

PTM databases

PhosphoSiteiQ6S5L9.

Expressioni

Tissue specificityi

Expressed in both brain and skeletal muscle; widely expressed in brain namely olfactory bulb, cortex, hippocampus, striatum, thalamus, and brain stem (at protein level). Only expressed in melanomas. Weakly expressed in normal melanocytes and benign nevi. Highly expressed at the transition from radial growth phase to vertical growth phase and metastatic melanomas, when tumor cells acquire migratory competence and invasive potential.1 Publication

Gene expression databases

BgeeiQ6S5L9.
ExpressionAtlasiQ6S5L9. baseline and differential.
GenevisibleiQ6S5L9. MM.

Interactioni

Subunit structurei

Interacts (via PID domain) with phosphorylated MUSK (via NPXY motif); undergoes tyrosine phosphorylation downstream of activated MUSK. Interacts with GRB2; the interaction is dependent of Tyr-422 phosphorylation and increased by EGF (By similarity).By similarity

GO - Molecular functioni

  • protein domain specific binding Source: MGI
  • receptor tyrosine kinase binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043146.

Structurei

3D structure databases

ProteinModelPortaliQ6S5L9.
SMRiQ6S5L9. Positions 181-380, 488-615.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini186 – 369184PIDPROSITE-ProRule annotationAdd
BLAST
Domaini522 – 61392SH2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 185185CH2Add
BLAST
Regioni370 – 521152CH1Add
BLAST

Sequence similaritiesi

Contains 1 PID domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG3697. Eukaryota.
ENOG410XTJN. LUCA.
GeneTreeiENSGT00390000018860.
HOGENOMiHOG000231974.
HOVERGENiHBG050121.
InParanoidiQ6S5L9.
KOiK17449.
OMAiPRMASMK.
OrthoDBiEOG7MD4QK.
PhylomeDBiQ6S5L9.
TreeFamiTF315807.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR006019. PID_Shc-like.
IPR006020. PTB/PI_dom.
IPR000980. SH2.
IPR029596. SHC4.
[Graphical view]
PANTHERiPTHR10337:SF12. PTHR10337:SF12. 1 hit.
PfamiPF00640. PID. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00629. SHCPIDOMAIN.
SMARTiSM00462. PTB. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6S5L9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRERSQDSQA GLTLYVGLFG HLGMLHRTKY SRFRNESITS LDEGSPGGSV
60 70 80 90 100
GNKGSSPPPY PALAPHLPTE DATVSSQESP TALCTLIPRM ASMKLANPIT
110 120 130 140 150
FLGLKTFCLG TKQVSRLKLQ ENQDQTPSRP ASPESNLNRT GPAPAPDPDQ
160 170 180 190 200
VGRRPTSLRP DTCPLPGPGE PSPRSKQDGP PLQHLLGNGL NYCVRYMGCI
210 220 230 240 250
EVLQSMRSLD FGMRTQVTRE AISRLCEAVP GAHGAIKKRK APVKFLTTVL
260 270 280 290 300
GKSNLQFSGM NIKLTVSTSS LTLMNLDNQQ IIANHQMQSI SFASGGDPDT
310 320 330 340 350
TDYVAYVAKD PVNQRACHIL ECRSGMAQDV ISTIGQAFEL RFKQYLKNPS
360 370 380 390 400
LNTWEREEVL VDGAPEDRDH DYYNSIPGKQ PPEGGISDVR IQAQATDQMA
410 420 430 440 450
YCPIRCEKLC YLPGNSTCSG VYKNCMGRSR PIGIPHERAG QGDTPSLRHF
460 470 480 490 500
WRVDLFDDPC YVNTQALQSM HSYAGNQSSA LPQGSPWHLG KAPETVQPGA
510 520 530 540 550
TAKPGSALAL PHIRQQLWDE ECFHGKLSRG AAEKLLVKDG DFLVRESVTS
560 570 580 590 600
PGQFVLSGLQ GGQAKHLLLV DPEGKVRTKD HVFDNVGHLI KYHMDNNLPI
610 620
ISSGSEVRLK QPIRKYDNTG LLPPKK
Length:626
Mass (Da):68,519
Last modified:July 5, 2004 - v1
Checksum:i031D64EA64D8F0FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY464564 mRNA. Translation: AAR19362.1.
AL929166, AL844580 Genomic DNA. Translation: CAM20855.1.
AL844580, AL929166 Genomic DNA. Translation: CAM22354.1.
CCDSiCCDS16678.1.
RefSeqiNP_950187.1. NM_199022.2.
UniGeneiMm.425101.
Mm.450761.

Genome annotation databases

EnsembliENSMUST00000042246; ENSMUSP00000043146; ENSMUSG00000035109.
GeneIDi271849.
KEGGimmu:271849.
UCSCiuc008mcv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY464564 mRNA. Translation: AAR19362.1.
AL929166, AL844580 Genomic DNA. Translation: CAM20855.1.
AL844580, AL929166 Genomic DNA. Translation: CAM22354.1.
CCDSiCCDS16678.1.
RefSeqiNP_950187.1. NM_199022.2.
UniGeneiMm.425101.
Mm.450761.

3D structure databases

ProteinModelPortaliQ6S5L9.
SMRiQ6S5L9. Positions 181-380, 488-615.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043146.

PTM databases

PhosphoSiteiQ6S5L9.

Proteomic databases

PaxDbiQ6S5L9.
PRIDEiQ6S5L9.

Protocols and materials databases

DNASUi271849.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042246; ENSMUSP00000043146; ENSMUSG00000035109.
GeneIDi271849.
KEGGimmu:271849.
UCSCiuc008mcv.1. mouse.

Organism-specific databases

CTDi399694.
MGIiMGI:2655364. Shc4.

Phylogenomic databases

eggNOGiKOG3697. Eukaryota.
ENOG410XTJN. LUCA.
GeneTreeiENSGT00390000018860.
HOGENOMiHOG000231974.
HOVERGENiHBG050121.
InParanoidiQ6S5L9.
KOiK17449.
OMAiPRMASMK.
OrthoDBiEOG7MD4QK.
PhylomeDBiQ6S5L9.
TreeFamiTF315807.

Miscellaneous databases

ChiTaRSiShc4. mouse.
NextBioi393518.
PROiQ6S5L9.
SOURCEiSearch...

Gene expression databases

BgeeiQ6S5L9.
ExpressionAtlasiQ6S5L9. baseline and differential.
GenevisibleiQ6S5L9. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR006019. PID_Shc-like.
IPR006020. PTB/PI_dom.
IPR000980. SH2.
IPR029596. SHC4.
[Graphical view]
PANTHERiPTHR10337:SF12. PTHR10337:SF12. 1 hit.
PfamiPF00640. PID. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00629. SHCPIDOMAIN.
SMARTiSM00462. PTB. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RaLP, a new member of the Src homology and collagen family, regulates cell migration and tumor growth of metastatic melanomas."
    Fagiani E., Giardina G., Luzi L., Cesaroni M., Quarto M., Capra M., Germano G., Bono M., Capillo M., Pelicci P., Lanfrancone L.
    Cancer Res. 67:3064-3073(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Analysis of a Shc family adaptor protein, ShcD/Shc4, that associates with muscle-specific kinase."
    Jones N., Hardy W.R., Friese M.B., Jorgensen C., Smith M.J., Woody N.M., Burden S.J., Pawson T.
    Mol. Cell. Biol. 27:4759-4773(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSHC4_MOUSE
AccessioniPrimary (citable) accession number: Q6S5L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.