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Protein

Long-sarafotoxin

Gene
N/A
Organism
Atractaspis microlepidota microlepidota
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.1 Publication
Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei2 – 21Endothelin-receptor binding siteBy similarity
Sitei50 – 501Endothelin-receptor binding siteBy similarity
Sitei98 – 981Endothelin-receptor binding siteBy similarity
Sitei146 – 1461Endothelin-receptor binding siteBy similarity
Sitei194 – 1941Endothelin-receptor binding siteBy similarity
Sitei242 – 2421Endothelin-receptor binding siteBy similarity
Sitei290 – 2901Endothelin-receptor binding siteBy similarity
Sitei338 – 3381Endothelin-receptor binding siteBy similarity

GO - Biological processi

  1. regulation of vasoconstriction Source: InterPro
  2. vasoconstriction Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Cardiotoxin, G-protein coupled receptor impairing toxin, Toxin, Vasoactive, Vasoconstrictor

Names & Taxonomyi

Protein namesi
Recommended name:
Long-sarafotoxin
Short name:
L-SRTX
Cleaved into the following 6 chains:
Sarafotoxin-m
Short name:
SRTX-m
Sarafotoxin-m1
Short name:
SRTX-m1
Sarafotoxin-m2
Short name:
SRTX-m2
Sarafotoxin-m3
Short name:
SRTX-m3
Sarafotoxin-m4
Short name:
SRTX-m4
Sarafotoxin-m5
Short name:
SRTX-m5
OrganismiAtractaspis microlepidota microlepidota
Taxonomic identifieri172021 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaLamprophiidaeAtractaspidinaeAtractaspis

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

Sarafotoxin-m: LD50 is 27-37 µg/kg by intravenous injection into mice.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi51 – 533Missing: Drastic 4-orders or magnitude increase in affinity for ET-B receptors. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei‹1 – 5›5Sarafotoxin-m5PRO_0000315373
Propeptidei6 – 29241 PublicationPRO_0000315374Add
BLAST
Peptidei30 – 5324Sarafotoxin-mPRO_5000092481Add
BLAST
Propeptidei54 – 77241 PublicationPRO_0000315375Add
BLAST
Peptidei78 – 10124Sarafotoxin-m1PRO_5000092482Add
BLAST
Propeptidei102 – 125241 PublicationPRO_0000315376Add
BLAST
Peptidei126 – 14924Sarafotoxin-m3PRO_5000092483Add
BLAST
Propeptidei150 – 173241 PublicationPRO_0000315377Add
BLAST
Peptidei174 – 19724Sarafotoxin-m2PRO_5000092484Add
BLAST
Propeptidei198 – 221241 PublicationPRO_0000315378Add
BLAST
Peptidei222 – 24524Sarafotoxin-m2PRO_5000092485Add
BLAST
Propeptidei246 – 269241 PublicationPRO_0000315379Add
BLAST
Peptidei270 – 29324Sarafotoxin-mPRO_5000092486Add
BLAST
Propeptidei294 – 317241 PublicationPRO_0000315380Add
BLAST
Peptidei318 – 34124Sarafotoxin-m4PRO_5000092487Add
BLAST
Propeptidei342 – 35110PRO_0000315381

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 441 Publication
Disulfide bondi32 ↔ 401 Publication
Disulfide bondi78 ↔ 92By similarity
Disulfide bondi80 ↔ 88By similarity
Disulfide bondi126 ↔ 140By similarity
Disulfide bondi128 ↔ 136By similarity
Disulfide bondi174 ↔ 188By similarity
Disulfide bondi176 ↔ 184By similarity
Disulfide bondi222 ↔ 236By similarity
Disulfide bondi224 ↔ 232By similarity
Disulfide bondi270 ↔ 284By similarity
Disulfide bondi272 ↔ 280By similarity
Disulfide bondi318 ↔ 332By similarity
Disulfide bondi320 ↔ 328By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

Secondary structure

1
351
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi273 – 2753Combined sources
Helixi277 – 28610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LDFNMR-A270-293[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6RY98.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati6 – 53481Add
BLAST
Repeati54 – 101482Add
BLAST
Repeati102 – 149483Add
BLAST
Repeati150 – 197484Add
BLAST
Repeati198 – 245485Add
BLAST
Repeati246 – 293486Add
BLAST
Repeati294 – 341487Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 3413367 X 48 AA tandem repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the endothelin/sarafotoxin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG017852.

Family and domain databases

InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 7 hits.
[Graphical view]
PRINTSiPR00365. ENDOTHELIN.
SMARTiSM00272. END. 7 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6RY98-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
IWDEPVVVSA RDTEEAARVP SPQKRSQPLC SCNDINDKEC MYFCHQDVIW
60 70 80 90 100
DEPVVVSVRD TEEAARVPSP QKRPQPRCSC NDMNDKECMY FCHQDVIWDE
110 120 130 140 150
PVVVSVRDTE EAARVPSPQK RSQPRCSCND MNDKECVYFC HLDIIWDEPV
160 170 180 190 200
VVSVRDTEEA TRVPSPQKRS QPLCSCNDIN DKECMYFCHQ DIIWDEPVVV
210 220 230 240 250
SVRDTEEAAR VPSPQKRSQP LCSCNDINDK ECMYFCHQDI IWDEPVVVSV
260 270 280 290 300
RDTEEAARVP SPQKRSQPLC SCNDINDKEC MYFCHQDVIW DEPVVVSVQD
310 320 330 340 350
TEEAARVPSP QKRSQPLCSC NNMSDKECLN FCNLDIIWEN VDTSADPEFL

G
Length:351
Mass (Da):40,341
Last modified:July 5, 2004 - v1
Checksum:iF901846C8D6E01CC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY485934 mRNA. Translation: AAR84382.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY485934 mRNA. Translation: AAR84382.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LDFNMR-A270-293[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG017852.

Miscellaneous databases

EvolutionaryTraceiQ6RY98.

Family and domain databases

InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 7 hits.
[Graphical view]
PRINTSiPR00365. ENDOTHELIN.
SMARTiSM00272. END. 7 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-53; 78-101; 126-149; 174-197; 222-245; 270-293 AND 318-341, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, LETHAL DOSE, SYNTHESIS OF SARAFOTOXIN-M, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Venom and Venom gland.
  2. "Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: role of the C-terminus extension."
    Mourier G., Hajj M., Cordier F., Zorba A., Gao X., Coskun T., Herbet A., Marcon E., Beau F., Delepierre M., Ducancel F., Servent D.
    Biochimie 94:461-470(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF SARAFOTOXIN-M, DISULFIDE BONDS, MUTAGENESIS OF 51-ASP--PRO-53.

Entry informationi

Entry nameiSRTXL_ATRMM
AccessioniPrimary (citable) accession number: Q6RY98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.