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Q6RY98

- SRTXL_ATRMM

UniProt

Q6RY98 - SRTXL_ATRMM

Protein

Long-sarafotoxin

Gene
N/A
Organism
Atractaspis microlepidota microlepidota
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.1 Publication
    Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm By similarity. Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).By similarity2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei2 – 21Endothelin-receptor binding siteBy similarity
    Sitei50 – 501Endothelin-receptor binding siteBy similarity
    Sitei98 – 981Endothelin-receptor binding siteBy similarity
    Sitei146 – 1461Endothelin-receptor binding siteBy similarity
    Sitei194 – 1941Endothelin-receptor binding siteBy similarity
    Sitei242 – 2421Endothelin-receptor binding siteBy similarity
    Sitei290 – 2901Endothelin-receptor binding siteBy similarity
    Sitei338 – 3381Endothelin-receptor binding siteBy similarity

    GO - Biological processi

    1. regulation of vasoconstriction Source: InterPro
    2. vasoconstriction Source: UniProtKB-KW

    Keywords - Molecular functioni

    Cardiotoxin, G-protein coupled receptor impairing toxin, Toxin, Vasoactive, Vasoconstrictor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long-sarafotoxin
    Short name:
    L-SRTX
    Cleaved into the following 6 chains:
    Sarafotoxin-m
    Short name:
    SRTX-m
    Sarafotoxin-m1
    Short name:
    SRTX-m1
    Sarafotoxin-m2
    Short name:
    SRTX-m2
    Sarafotoxin-m3
    Short name:
    SRTX-m3
    Sarafotoxin-m4
    Short name:
    SRTX-m4
    Sarafotoxin-m5
    Short name:
    SRTX-m5
    OrganismiAtractaspis microlepidota microlepidota
    Taxonomic identifieri172021 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaAtractaspididaeAtractaspis

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Toxic dosei

    Sarafotoxin-m: LD50 is 27-37 µg/kg by intravenous injection into mice.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi51 – 533Missing: Drastic 4-orders or magnitude increase in affinity for ET-B receptors. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Peptidei‹1 – 5›5Sarafotoxin-m5PRO_0000315373
    Propeptidei6 – 29241 PublicationPRO_0000315374Add
    BLAST
    Peptidei30 – 5324Sarafotoxin-mPRO_5000092481Add
    BLAST
    Propeptidei54 – 77241 PublicationPRO_0000315375Add
    BLAST
    Peptidei78 – 10124Sarafotoxin-m1PRO_5000092482Add
    BLAST
    Propeptidei102 – 125241 PublicationPRO_0000315376Add
    BLAST
    Peptidei126 – 14924Sarafotoxin-m3PRO_5000092483Add
    BLAST
    Propeptidei150 – 173241 PublicationPRO_0000315377Add
    BLAST
    Peptidei174 – 19724Sarafotoxin-m2PRO_5000092484Add
    BLAST
    Propeptidei198 – 221241 PublicationPRO_0000315378Add
    BLAST
    Peptidei222 – 24524Sarafotoxin-m2PRO_5000092485Add
    BLAST
    Propeptidei246 – 269241 PublicationPRO_0000315379Add
    BLAST
    Peptidei270 – 29324Sarafotoxin-mPRO_5000092486Add
    BLAST
    Propeptidei294 – 317241 PublicationPRO_0000315380Add
    BLAST
    Peptidei318 – 34124Sarafotoxin-m4PRO_5000092487Add
    BLAST
    Propeptidei342 – 35110PRO_0000315381

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 441 Publication
    Disulfide bondi32 ↔ 401 Publication
    Disulfide bondi78 ↔ 92By similarity
    Disulfide bondi80 ↔ 88By similarity
    Disulfide bondi126 ↔ 140By similarity
    Disulfide bondi128 ↔ 136By similarity
    Disulfide bondi174 ↔ 188By similarity
    Disulfide bondi176 ↔ 184By similarity
    Disulfide bondi222 ↔ 236By similarity
    Disulfide bondi224 ↔ 232By similarity
    Disulfide bondi270 ↔ 284By similarity
    Disulfide bondi272 ↔ 280By similarity
    Disulfide bondi318 ↔ 332By similarity
    Disulfide bondi320 ↔ 328By similarity

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.1 Publication

    Structurei

    Secondary structure

    1
    351
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi273 – 2753
    Helixi277 – 28610

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LDFNMR-A270-293[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6RY98.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati6 – 53481Add
    BLAST
    Repeati54 – 101482Add
    BLAST
    Repeati102 – 149483Add
    BLAST
    Repeati150 – 197484Add
    BLAST
    Repeati198 – 245485Add
    BLAST
    Repeati246 – 293486Add
    BLAST
    Repeati294 – 341487Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni6 – 3413367 X 48 AA tandem repeatsAdd
    BLAST

    Sequence similaritiesi

    Belongs to the endothelin/sarafotoxin family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG017852.

    Family and domain databases

    InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
    IPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    [Graphical view]
    PfamiPF00322. Endothelin. 7 hits.
    [Graphical view]
    PRINTSiPR00365. ENDOTHELIN.
    SMARTiSM00272. END. 7 hits.
    [Graphical view]
    PROSITEiPS00270. ENDOTHELIN. 7 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6RY98-1 [UniParc]FASTAAdd to Basket

    « Hide

    IWDEPVVVSA RDTEEAARVP SPQKRSQPLC SCNDINDKEC MYFCHQDVIW    50
    DEPVVVSVRD TEEAARVPSP QKRPQPRCSC NDMNDKECMY FCHQDVIWDE 100
    PVVVSVRDTE EAARVPSPQK RSQPRCSCND MNDKECVYFC HLDIIWDEPV 150
    VVSVRDTEEA TRVPSPQKRS QPLCSCNDIN DKECMYFCHQ DIIWDEPVVV 200
    SVRDTEEAAR VPSPQKRSQP LCSCNDINDK ECMYFCHQDI IWDEPVVVSV 250
    RDTEEAARVP SPQKRSQPLC SCNDINDKEC MYFCHQDVIW DEPVVVSVQD 300
    TEEAARVPSP QKRSQPLCSC NNMSDKECLN FCNLDIIWEN VDTSADPEFL 350
    G 351
    Length:351
    Mass (Da):40,341
    Last modified:July 5, 2004 - v1
    Checksum:iF901846C8D6E01CC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY485934 mRNA. Translation: AAR84382.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY485934 mRNA. Translation: AAR84382.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LDF NMR - A 270-293 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG017852.

    Miscellaneous databases

    EvolutionaryTracei Q6RY98.

    Family and domain databases

    InterProi IPR020475. Bibrotoxin/Sarafotoxin-D.
    IPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    [Graphical view ]
    Pfami PF00322. Endothelin. 7 hits.
    [Graphical view ]
    PRINTSi PR00365. ENDOTHELIN.
    SMARTi SM00272. END. 7 hits.
    [Graphical view ]
    PROSITEi PS00270. ENDOTHELIN. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-53; 78-101; 126-149; 174-197; 222-245; 270-293 AND 318-341, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, LETHAL DOSE, SYNTHESIS OF SARAFOTOXIN-M, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Venom and Venom gland.
    2. "Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: role of the C-terminus extension."
      Mourier G., Hajj M., Cordier F., Zorba A., Gao X., Coskun T., Herbet A., Marcon E., Beau F., Delepierre M., Ducancel F., Servent D.
      Biochimie 94:461-470(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF SARAFOTOXIN-M, DISULFIDE BONDS, MUTAGENESIS OF 51-ASP--PRO-53.

    Entry informationi

    Entry nameiSRTXL_ATRMM
    AccessioniPrimary (citable) accession number: Q6RY98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3