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Q6RY07 (CHIA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Acidic mammalian chitinase
Short name=AMCase
EC=3.2.1.14
Gene names
Name:Chia
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding By similarity.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Subunit structure

Interacts with EGFR By similarity.

Subcellular location

Cytoplasm By similarity. Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Contains 1 chitin-binding type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 473452Acidic mammalian chitinase
PRO_0000011946

Regions

Domain424 – 47350Chitin-binding type-2
Region70 – 712Chitooligosaccharide binding By similarity
Region97 – 1004Chitooligosaccharide binding By similarity
Region210 – 2134Chitooligosaccharide binding By similarity
Compositional bias409 – 42517Gly/Ser-rich

Sites

Active site1401Proton donor By similarity
Binding site1411Chitooligosaccharide By similarity
Binding site3601Chitooligosaccharide By similarity

Amino acid modifications

Disulfide bond26 ↔ 51 By similarity
Disulfide bond49 ↔ 394 By similarity
Disulfide bond307 ↔ 372 By similarity
Disulfide bond457 ↔ 470 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6RY07 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: FBB0DB91A42C1EFD

FASTA47351,951
        10         20         30         40         50         60 
MAKLILVTGL VLLLNVQLGS AYNLVCYFTN WAQYRPGLGS FKPDDINPCL CTHLIYAFAG 

        70         80         90        100        110        120 
MQNNQITTIE WNDVTLYKAF NDLKNRNSKL KTLLAIGGWN FGTAPFTTMV STSQNRQTFI 

       130        140        150        160        170        180 
TSVIKFLRQY GFDGLDLDWE YPGSRGSPPQ DKHLFTVLVK ELREAFEQEA IESNRPRLMV 

       190        200        210        220        230        240 
TAAVAAGISN IQAGYEIPEL SQYLDFIHVM TYDLHGSWDG YTGENSPLYK LPTETGSNAY 

       250        260        270        280        290        300 
LNVDYVMNYW KDNGAPAEKL IVGFPEYGHT YILSNPSDTG IGAPTSGNGP AGPYTRQAGF 

       310        320        330        340        350        360 
WAYYEICTFL RNGATQDWDA PQEVPYAYKG NEWVGYDNIK SFSVKAQWLK QNNFGGAMIW 

       370        380        390        400        410        420 
AIDLDDFTGS FCDQGKFPLT STLNKALDIP TAGCTAPDLP SEPVTTPPGS GSGGGSSGGG 

       430        440        450        460        470 
SEGSGFCAGK ADGLYPVADD RNAFWHCING ITYQQHCQAG LVFDTSCNCC NWP

« Hide

References

[1]"Rattus norvegicus similar to acidic mammalian chitinase precursor."
Chen X.-H., Cai G.-P.
Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Stomach.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY486074 mRNA. Translation: AAR28968.1.
IPIIPI00421295.
RefSeqNP_997469.1. NM_207586.1.
UniGeneRn.43692.

3D structure databases

ProteinModelPortalQ6RY07.
SMRQ6RY07. Positions 22-394.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ6RY07.

Protein family/group databases

CAZyCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Proteomic databases

PRIDEQ6RY07.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID113901.
KEGGrno:113901.

Organism-specific databases

CTD27159.
RGD1303058. Chia.

Phylogenomic databases

eggNOGroNOG10317.
GeneTreeENSGT00550000074323.
HOVERGENHBG011684.
OrthoDBEOG476K16.
PhylomeDBQ6RY07.

Gene expression databases

ArrayExpressQ6RY07.
GenevestigatorQ6RY07.
GermOnlineENSRNOG00000033162. Rattus norvegicus.

Family and domain databases

InterProIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:2.170.140.10. Chitin-bd_dom. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
KOK01183.
PfamPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMSSF57625. Chitin_bind_PerA. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio617982.

Entry information

Entry nameCHIA_RAT
AccessionPrimary (citable) accession number: Q6RY07
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 5, 2004
Last modified: November 16, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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