Reviewed, UniProtKB/Swiss-Prot Q6RW13 (ATRAP_HUMAN)

Names and origin

Protein names

Recommended name:
    Type-1 angiotensin II receptor-associated protein
Alternative name(s):
    AT1 receptor-associated protein

Gene names

Name:	AGTRAP
Synonyms:	ATRAP

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalisation as well as mechanism of receptor desensitization such as phosphorylation. Induces also a decrease in cell proliferation and angiotensin II-stimulated transcriptional activity. Ref.6
Subunit structure	Interacts with GNB2L1/RACK1, and with the C-terminal region of AGTR1. Ref.6 Ref.1
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Present in perinuclear vesicular membranes, Endoplasmic reticulum, Golgi and endocytic vesicles. Ref.6
Tissue specificity	Ubiquitous but more abundant in kidney, heart, pancreas and thyroid. Ref.1
Sequence caution	The sequence CAI15876.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI15877.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PBX3	Q96AL5	2	EBI-741181,EBI-741171
PITPNC1	Q9UKF7	2	EBI-741181,EBI-749241

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6RW13-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6RW13-2)

The sequence of this isoform differs from the canonical sequence as follows:
     115-121: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 159	159	Type-1 angiotensin II receptor-associated protein		PRO_0000064735
Regions
	Topological domain	1 – 23	23	Extracellular Potential
	Transmembrane	24 – 44	21	Potential
	Topological domain	45 – 55	11	Cytoplasmic Potential
	Transmembrane	56 – 76	21	Potential
	Topological domain	77 – 86	10	Extracellular Potential
	Transmembrane	87 – 107	21	Potential
	Topological domain	108 – 159	52	Cytoplasmic Potential
	Region	110 – 122	13	Interaction with AGTR1
Natural variations
	Alternative sequence	115 – 121	7	Missing in isoform 2.		VSP_014839
	Natural variant	143	1	A → V: dbSNP rs17875960. Ref.3		VAR_023075
Experimental info
	Sequence conflict	158	1	G → R in AAF89547. Ref.2
	Sequence conflict	159	1	Y → S in AAL26806. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 7E1D5C7E79AE6BC5	FASTA	159	17,419
	10 20 30 40 50 60 MELPAVNLKV ILLGHWLLTT WGCIVFSGSY AWANFTILAL GVWAVAQRDS IDAISMFLGG 70 80 90 100 110 120 LLATIFLDIV HISIFYPRVS LTDTGRFGVG MAILSLLLKP LSCCFVYHMY RERGGELLVH 130 140 150 TGFLGSSQDR SAYQTIDSAE APADPFAVPE GRSQDARGY
	Isoform 2. Checksum: 637C01214175C3C9	FASTA	152	16,669
	10 20 30 40 50 60 MELPAVNLKV ILLGHWLLTT WGCIVFSGSY AWANFTILAL GVWAVAQRDS IDAISMFLGG 70 80 90 100 110 120 LLATIFLDIV HISIFYPRVS LTDTGRFGVG MAILSLLLKP LSCCFVYHMY RERGGFLGSS 130 140 150 QDRSAYQTID SAEAPADPFA VPEGRSQDAR GY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of AGTRAP, a human homolog of murine angiotensin II receptor-associated protein (Agtrap)." Wang W., Huang Y., Zhou Z., Tang R., Zhao W., Zeng L., Xu M., Cheng C., Gu S., Ying K., Xie Y., Mao Y. Int. J. Biochem. Cell Biol. 34:93-102(2002) [PubMed: 11733189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH GNB2L1 AND AGTR1. Tissue: Fetal brain.
[2]	Ye R.D., He R. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Kidney.
[3]	SeattleSNPs variation discovery resource Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-143.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Colon.
[6]	"The angiotensin II type I receptor-associated protein, ATRAP, is a transmembrane protein and a modulator of angiotensin II signaling." Lopez-Ilasaca M., Liu X., Tamura K., Dzau V.J. Mol. Biol. Cell 14:5038-5050(2003) [PubMed: 12960423] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH AGTR1.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF312374 mRNA. Translation: AAL26806.1. AF165187 mRNA. Translation: AAF89547.1. AY488088 Genomic DNA. Translation: AAR25556.1. AL953897 Genomic DNA. Translation: CAI15876.1. Sequence problems. AL953897 Genomic DNA. Translation: CAI15877.1. Sequence problems. AL953897 Genomic DNA. Translation: CAI15878.1. AL953897 Genomic DNA. Translation: CAI15879.1. BC017328 mRNA. Translation: AAH17328.1.
IPI	IPI00102501. IPI00513986.
RefSeq	NP_001035284.1. NP_001035285.1. NP_001035286.1. NP_001035287.1. NP_065083.3.
UniGene	Hs.464438
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6RW13. 3 interactions.
STRING	Q6RW13.
PTM databases
PhosphoSite	Q6RW13.
Proteomic databases
PeptideAtlas	Q6RW13.
PRIDE	Q6RW13.
Genome annotation databases
Ensembl	ENST00000314340; ENSP00000319713; ENSG00000177674; Homo sapiens. [Genome view]
GeneID	57085.
KEGG	hsa:57085.
UCSC	uc001asv.1. human. uc001asw.1. human.
Organism-specific databases
CTD	57085.
GeneCards	GC01P011730.
H-InvDB	HIX0020154.
HGNC	HGNC:13539. AGTRAP.
MIM	608729. gene.
PharmGKB	PA38363.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q6RW13.
OMA	WAVAQRD
OrthoDB	EOG9C2KX6
Gene expression databases
ArrayExpress	Q6RW13.
Bgee	Q6RW13.
CleanEx	HS_AGTRAP.
Genevestigator	Q6RW13.
GermOnline	ENSG00000177674. Homo sapiens.
Family and domain databases
InterPro	IPR009436. Angiotensin_II_typ1_rcpt-assoc. IPR013339. Angiotensin_II_typ1_rcpt-rel. [Graphical view]
Pfam	PF06396. AGTRAP. 1 hit. [Graphical view]
SMART	SM00805. AGTRAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	62875.
SOURCE	Search...

Entry information

Entry name

ATRAP_HUMAN

Accession

Primary (citable) accession number: Q6RW13
Secondary accession number(s): Q5SNV4 , Q5SNV5, Q96AC0, Q96PL4, Q9NRW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	November 24, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.