Q6RW13 (ATRAP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-1 angiotensin II receptor-associated protein

Alternative name(s):
AT1 receptor-associated protein

Gene names

Name:	AGTRAP
Synonyms:	ATRAP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalisation as well as mechanism of receptor desensitization such as phosphorylation. Induces also a decrease in cell proliferation and angiotensin II-stimulated transcriptional activity. Ref.8
Subunit structure	Interacts with GNB2L1/RACK1, and with the C-terminal region of AGTR1. Ref.1 Ref.8
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Present in perinuclear vesicular membranes, Endoplasmic reticulum, Golgi and endocytic vesicles. Ref.8
Tissue specificity	Ubiquitous but more abundant in kidney, heart, pancreas and thyroid. Ref.1
Sequence caution	The sequence CAI15876.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of blood pressure Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell cortex Inferred from electronic annotation. Source: Compara cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	angiotensin type II receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PBX3	Q96AL5	3	EBI-741181,EBI-741171
PITPNC1	Q9UKF7	3	EBI-741181,EBI-749241

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6RW13-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6RW13-2)

The sequence of this isoform differs from the canonical sequence as follows:
     115-121: Missing.

Isoform 3 (identifier: Q6RW13-3)

The sequence of this isoform differs from the canonical sequence as follows:
     21-159: WGCIVFSGSY...EGRSQDARGY → CFWRHFSAKP...RLPHVPGARG

Note: No experimental confirmation available.

Isoform 5 (identifier: Q6RW13-5)

The sequence of this isoform differs from the canonical sequence as follows:
     21-159: WGCIVFSGSY...EGRSQDARGY → CFWRHFSAKP...RGFPWVFSGP

Isoform 4 (identifier: Q6RW13-4)

The sequence of this isoform differs from the canonical sequence as follows:
     10-159: VILLGHWLLT...EGRSQDARGY → GLHCILRLLC...RGFPWVFSGP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 159	159	Type-1 angiotensin II receptor-associated protein		PRO_0000064735
Regions
	Topological domain	1 – 23	23	Extracellular Potential
	Transmembrane	24 – 44	21	Helical; Potential
	Topological domain	45 – 55	11	Cytoplasmic Potential
	Transmembrane	56 – 76	21	Helical; Potential
	Topological domain	77 – 86	10	Extracellular Potential
	Transmembrane	87 – 107	21	Helical; Potential
	Topological domain	108 – 159	52	Cytoplasmic Potential
	Region	110 – 122	13	Interaction with AGTR1
Natural variations
	Alternative sequence	10 – 159	150	VILLG…DARGY → GLHCILRLLCLGQLHHPGLG RVGCGSAGLHRRHKHVSGWL AGHHLPGHRAHQHLLPAGQP HGHGPLWRGHGHPQLAAQAA LLLLRLPHVPGARGFPWVFS GP in isoform 4.		VSP_039291
	Alternative sequence	21 – 159	139	WGCIV…DARGY → CFWRHFSAKPRLETIELTCA LCKLRSAAHRATAGLHCILR LLCLGQLHHPGLGRVGCGSA GLHRRHKHVSGWLAGHHLPG HRAHQHLLPAGQPHGHGPLW RGHGHPQLAAQAALLLLRLP HVPGARG in isoform 3.		VSP_039290
	Alternative sequence	21 – 159	139	WGCIV…DARGY → CFWRHFSAKPRLETIELTCA LCKLRSAAHRATAGLHCILR LLCLGQLHHPGLGRVGCGSA GLHRRHKHVSGWLAGHHLPG HRAHQHLLPAGQPHGHGPLW RGHGHPQLAAQAALLLLRLP HVPGARGFPWVFSGP in isoform 5.		VSP_040406
	Alternative sequence	115 – 121	7	Missing in isoform 2.		VSP_014839
	Natural variant	143	1	A → V. Ref.4 Corresponds to variant rs17875960 [ dbSNP | Ensembl ].		VAR_023075
Experimental info
	Sequence conflict	158	1	G → R in AAF89547. Ref.2
	Sequence conflict	159	1	Y → S in AAL26806. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 7E1D5C7E79AE6BC5	FASTA	159	17,419
	10 20 30 40 50 60 MELPAVNLKV ILLGHWLLTT WGCIVFSGSY AWANFTILAL GVWAVAQRDS IDAISMFLGG 70 80 90 100 110 120 LLATIFLDIV HISIFYPRVS LTDTGRFGVG MAILSLLLKP LSCCFVYHMY RERGGELLVH 130 140 150 TGFLGSSQDR SAYQTIDSAE APADPFAVPE GRSQDARGY
	Isoform 2 [UniParc]. Checksum: 637C01214175C3C9	FASTA	152	16,669
	10 20 30 40 50 60 MELPAVNLKV ILLGHWLLTT WGCIVFSGSY AWANFTILAL GVWAVAQRDS IDAISMFLGG 70 80 90 100 110 120 LLATIFLDIV HISIFYPRVS LTDTGRFGVG MAILSLLLKP LSCCFVYHMY RERGGFLGSS 130 140 150 QDRSAYQTID SAEAPADPFA VPEGRSQDAR GY
	Isoform 3 [UniParc]. Checksum: 27B4716DE9C64CEC	FASTA	147	16,048
	10 20 30 40 50 60 MELPAVNLKV ILLGHWLLTT CFWRHFSAKP RLETIELTCA LCKLRSAAHR ATAGLHCILR 70 80 90 100 110 120 LLCLGQLHHP GLGRVGCGSA GLHRRHKHVS GWLAGHHLPG HRAHQHLLPA GQPHGHGPLW 130 140 RGHGHPQLAA QAALLLLRLP HVPGARG
	Isoform 5 [UniParc]. Checksum: C78BE559AC52A0A0	FASTA	155	16,966
	10 20 30 40 50 60 MELPAVNLKV ILLGHWLLTT CFWRHFSAKP RLETIELTCA LCKLRSAAHR ATAGLHCILR 70 80 90 100 110 120 LLCLGQLHHP GLGRVGCGSA GLHRRHKHVS GWLAGHHLPG HRAHQHLLPA GQPHGHGPLW 130 140 150 RGHGHPQLAA QAALLLLRLP HVPGARGFPW VFSGP
	Isoform 4 [UniParc]. Checksum: 5ECC78C7CD1D332B	FASTA	111	11,948
	10 20 30 40 50 60 MELPAVNLKG LHCILRLLCL GQLHHPGLGR VGCGSAGLHR RHKHVSGWLA GHHLPGHRAH 70 80 90 100 110 QHLLPAGQPH GHGPLWRGHG HPQLAAQAAL LLLRLPHVPG ARGFPWVFSG P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of AGTRAP, a human homolog of murine angiotensin II receptor-associated protein (Agtrap)." Wang W., Huang Y., Zhou Z., Tang R., Zhao W., Zeng L., Xu M., Cheng C., Gu S., Ying K., Xie Y., Mao Y. Int. J. Biochem. Cell Biol. 34:93-102(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH GNB2L1 AND AGTR1. Tissue: Fetal brain.
[2]	Ye R.D., He R. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Kidney.
[3]	Melton D., Brown J., Kenty G., Permutt A., Lee C., Kaestner K., Lemishka I., Scearce M., Brestelli J., Gradwohl G., Clifton S., Hillier L., Marra M., Pape D., Wylie T., Martin J., Blistain A., Schmitt A. , Theising B., Ritter E., Ronko I., Bennett J., Cardenas M., Gibbons M., McCann R., Cole R., Tsagareishvili R., Williams T., Jackson Y., Bowers Y. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Pancreas islet cell.
[4]	SeattleSNPs variation discovery resource Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-143.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 5). Tissue: Colon and Eye.
[8]	"The angiotensin II type I receptor-associated protein, ATRAP, is a transmembrane protein and a modulator of angiotensin II signaling." Lopez-Ilasaca M., Liu X., Tamura K., Dzau V.J. Mol. Biol. Cell 14:5038-5050(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH AGTR1.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF312374 mRNA. Translation: AAL26806.1. AF165187 mRNA. Translation: AAF89547.1. CA866314 mRNA. No translation available. AY488088 Genomic DNA. Translation: AAR25556.1. AL953897 Genomic DNA. Translation: CAI15876.1. Sequence problems. AL953897 Genomic DNA. Translation: CAI15877.1. AL953897 Genomic DNA. Translation: CAI15878.1. AL953897 Genomic DNA. Translation: CAI15879.1. CH471130 Genomic DNA. Translation: EAW71702.1. BC017328 mRNA. Translation: AAH17328.1. BE782705 mRNA. No translation available.
IPI	IPI00102501. IPI00513986. IPI00641159. IPI00743242. IPI01010838.
RefSeq	NP_001035284.1. NM_001040194.1. NP_001035285.1. NM_001040195.1. NP_001035286.1. NM_001040196.1. NP_001035287.1. NM_001040197.1. NP_065083.3. NM_020350.4.
UniGene	Hs.464438.
3D structure databases
ProteinModelPortal	Q6RW13.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6RW13. 2 interactions.
STRING	9606.ENSP00000319713.
PTM databases
PhosphoSite	Q6RW13.
Polymorphism databases
DMDM	71152303.
Proteomic databases
PaxDb	Q6RW13.
PeptideAtlas	Q6RW13.
PRIDE	Q6RW13.
Protocols and materials databases
DNASU	57085.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000314340; ENSP00000319713; ENSG00000177674. ENST00000376627; ENSP00000365814; ENSG00000177674. ENST00000376629; ENSP00000365816; ENSG00000177674. ENST00000376637; ENSP00000365824; ENSG00000177674. ENST00000400895; ENSP00000383688; ENSG00000177674. ENST00000452018; ENSP00000408505; ENSG00000177674.
GeneID	57085.
KEGG	hsa:57085.
UCSC	uc001ast.3. human. uc001asu.3. human. uc001asv.3. human. uc001asw.3. human. uc001asx.3. human.
Organism-specific databases
CTD	57085.
GeneCards	GC01P011796.
HGNC	HGNC:13539. AGTRAP.
HPA	HPA044120.
MIM	608729. gene.
neXtProt	NX_Q6RW13.
PharmGKB	PA38363.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG45277.
HOGENOM	HOG000034218.
HOVERGEN	HBG080881.
InParanoid	Q6RW13.
OMA	WAVAQRD.
PhylomeDB	Q6RW13.
Gene expression databases
ArrayExpress	Q6RW13.
Bgee	Q6RW13.
CleanEx	HS_AGTRAP.
Genevestigator	Q6RW13.
GermOnline	ENSG00000177674. Homo sapiens.
Family and domain databases
InterPro	IPR009436. Angiotensin_II_typ1_rcpt-assoc. [Graphical view]
Pfam	PF06396. AGTRAP. 1 hit. [Graphical view]
ProDom	PD357679. PD357679. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00805. AGTRAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AGTRAP. human.
GenomeRNAi	57085.
NextBio	62875.
SOURCE	Search...

Entry information

Entry name

ATRAP_HUMAN

Accession

Primary (citable) accession number: Q6RW13
Secondary accession number(s): A8MVQ5 , Q5SNV4, Q5SNV5, Q96AC0, Q96PL4, Q9NRW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.