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Protein

Electroneutral sodium bicarbonate exchanger 1

Gene

Slc4a8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates electroneutral sodium- and carbonate-dependent chloride-HCO3- exchange with a Na+:HCO3- stoichiometry of 2:1. Plays a major role in pH regulation in neurons. May be involved in cell pH regulation by transporting HCO3- from blood to cell. Enhanced expression in severe acid stress could be important for cell survival by mediating the influx of HCO3- into the cells. Also mediates lithium-dependent HCO3- cotransport. May be regulated by osmolarity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Anion exchange, Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-RNO-425381. Bicarbonate transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Electroneutral sodium bicarbonate exchanger 1
Alternative name(s):
Electroneutral Na+-driven Cl-HCO3 exchanger
Solute carrier family 4 member 8
k-NBC3
Gene namesi
Name:Slc4a8Imported
Synonyms:Ndcbe1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi735164. Slc4a8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 453ExtracellularSequence analysisAdd BLAST453
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Topological domaini475 – 482CytoplasmicSequence analysis8
Transmembranei483 – 503HelicalSequence analysisAdd BLAST21
Topological domaini504 – 540ExtracellularSequence analysisAdd BLAST37
Transmembranei541 – 561HelicalSequence analysisAdd BLAST21
Topological domaini562 – 570CytoplasmicSequence analysis9
Transmembranei571 – 591HelicalSequence analysisAdd BLAST21
Topological domaini592 – 662ExtracellularSequence analysisAdd BLAST71
Transmembranei663 – 683HelicalSequence analysisAdd BLAST21
Topological domaini684 – 706CytoplasmicSequence analysisAdd BLAST23
Transmembranei707 – 727HelicalSequence analysisAdd BLAST21
Topological domaini728 – 753ExtracellularSequence analysisAdd BLAST26
Transmembranei754 – 774HelicalSequence analysisAdd BLAST21
Topological domaini775 – 799CytoplasmicSequence analysisAdd BLAST25
Transmembranei800 – 820HelicalSequence analysisAdd BLAST21
Topological domaini821 – 856ExtracellularSequence analysisAdd BLAST36
Transmembranei857 – 877HelicalSequence analysisAdd BLAST21
Topological domaini878 – 879CytoplasmicSequence analysis2
Transmembranei880 – 900HelicalSequence analysisAdd BLAST21
Topological domaini901 – 937ExtracellularSequence analysisAdd BLAST37
Transmembranei938 – 958HelicalSequence analysisAdd BLAST21
Topological domaini959 – 1067CytoplasmicSequence analysisAdd BLAST109

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289241 – 1067Electroneutral sodium bicarbonate exchanger 1Add BLAST1067

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi611 ↔ 613By similarity
Disulfide bondi647 ↔ 659By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ6RVG2.
PRIDEiQ6RVG2.

PTM databases

iPTMnetiQ6RVG2.
PhosphoSitePlusiQ6RVG2.

Expressioni

Tissue specificityi

Expressed in the Purkinje cells and dendrites in the molecular layer of the cerebellum (at protein level). Strong expression observed in testis and moderate expression in kidney inner medulla, the submandibular gland, eye, cerebrum and cerebellum.1 Publication

Gene expression databases

ExpressionAtlasiQ6RVG2. differential.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043825.

Structurei

3D structure databases

ProteinModelPortaliQ6RVG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi998 – 1004Poly-GluSequence analysis7

Sequence similaritiesi

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
GeneTreeiENSGT00760000119021.
HOVERGENiHBG004326.
InParanoidiQ6RVG2.
KOiK13859.
PhylomeDBiQ6RVG2.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q6RVG2-1) [UniParc]FASTAAdd to basket
Also known as: NDCBE1-B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAGSNEPDG VLSYQRPDEE AVVDQGGTST ILNIHYEKEE LEGHRTLYVG
60 70 80 90 100
VRMPLGRQSH RHHRTHGQKH RRRGGRGKGA SQGEEGLEAS AHDTPSQRVQ
110 120 130 140 150
FILGTEEDEE HVPHELFTEL DEICMKEGEE AEWKETARWL KFEEDVEDGG
160 170 180 190 200
ERWSKPYVAT LSLHSLFELR SCLINGSVLL DMRASSIEEI SDLILDQQEL
210 220 230 240 250
LRDLSDSVRV KVREALLKKH HHQNEKKRNN LIPIVRSFAE VGKKQSDPHS
260 270 280 290 300
MDRDGQTVSP QSAPATNLEV KNGVNCEHSP VDLSKADLHF MKKIPAGAEA
310 320 330 340 350
SNVLVGEVDT LDRPIVAFVR LSPAVLLSGL TEVPIPTRFL FILLGPVGKG
360 370 380 390 400
QQYHEIGRSM ATIMTDEIFH DVAYKAKERD DLLAGIDEFL DQVTVLPPGE
410 420 430 440 450
WDPSIRIEPP KNVPSQEKRK MPGVPNGNIC HVEPEPHGGH SGPELERTGR
460 470 480 490 500
LQCLASFLFL YCACMSPVIT FGGLLGEATE GRISAIESLF GASMTGIAYS
510 520 530 540 550
LFAGQPLTIL GSTGPVLVFE KILFKFCKDY ALSYLSLRAC IGLWTAFLCI
560 570 580 590 600
VLVATDASSL VCYITRFTEE AFASLICIIF IYEAIEKLIH LAETYPIHMH
610 620 630 640 650
SQLDHLSLYY CRCALPENPN NHTLQYWKEH SIPTADVNWA NLTVSECQEM
660 670 680 690 700
HGEFIGSACG HHGPYTPDVL FWSCILFFAT FIVSSTLKTF KTSRYFPTRV
710 720 730 740 750
RSTVSDFAVF LTIFTMVILD FLIGVPSPKL QVPSVFKPTR DDRGWFISPI
760 770 780 790 800
GPNPWWTVIA AIIPALLCTI LIFMDQQITA VIINRKEHKL KKGCGYHLDL
810 820 830 840 850
LVVAIMLGVC SLMGLPWFVA ATVLSITHVN SLKLESECSA PGEQPKFLGI
860 870 880 890 900
REQRVTGLMI FVLMGCSVFM TAVLKFIPMP VLYGVFLYMG VSSLQGIQFF
910 920 930 940 950
DRLKLFGMPA KHQPDFIYLR HVPLRKVHLF TLVQLTCLVL LWVIKASPAA
960 970 980 990 1000
IVFPMMVLAL VFVRKVMDLC FSKRELSWLD DLMPESKKKK LDDAKKKEEE
1010 1020 1030 1040 1050
EEAEKMLDIG GDKFPLESRK LLSSPGKNNS FRCDPSEINI SDEMPKTTVW
1060
KALSINSGNT KEKSPFN
Length:1,067
Mass (Da):119,550
Last modified:July 5, 2004 - v1
Checksum:iBA8E72445DDB7749
GO
Isoform 1 (identifier: Q6RVG2-2) [UniParc]FASTAAdd to basket
Also known as: NDCBE1-A

The sequence of this isoform differs from the canonical sequence as follows:
     451-451: L → LFGGLVLDVKRKAPWYWSDYRDALSL
     646-699: Missing.
     1002-1002: Missing.

Show »
Length:1,037
Mass (Da):116,185
Checksum:i1EDB898C3BEBB8CC
GO
Isoform 3 (identifier: Q6RVG2-3) [UniParc]FASTAAdd to basket
Also known as: NDCBE1-C

The sequence of this isoform differs from the canonical sequence as follows:
     646-699: Missing.

Show »
Length:1,013
Mass (Da):113,375
Checksum:iCFD54321534C40B9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052766451L → LFGGLVLDVKRKAPWYWSDY RDALSL in isoform 1. 1 Publication1
Alternative sequenceiVSP_052767646 – 699Missing in isoform 1 and isoform 3. 1 PublicationAdd BLAST54
Alternative sequenceiVSP_0527681002Missing in isoform 1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY489024 mRNA. Translation: AAR37053.1.
AY489025 mRNA. Translation: AAR37054.1.
AY489026 mRNA. Translation: AAR37055.1.
RefSeqiNP_001257723.1. NM_001270794.1. [Q6RVG2-2]
NP_001257724.1. NM_001270795.1. [Q6RVG2-3]
NP_955791.1. NM_199497.2. [Q6RVG2-1]
UniGeneiRn.210164.

Genome annotation databases

EnsembliENSRNOT00000065379; ENSRNOP00000059220; ENSRNOG00000028879. [Q6RVG2-2]
GeneIDi315311.
KEGGirno:315311.
UCSCiRGD:735164. rat. [Q6RVG2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY489024 mRNA. Translation: AAR37053.1.
AY489025 mRNA. Translation: AAR37054.1.
AY489026 mRNA. Translation: AAR37055.1.
RefSeqiNP_001257723.1. NM_001270794.1. [Q6RVG2-2]
NP_001257724.1. NM_001270795.1. [Q6RVG2-3]
NP_955791.1. NM_199497.2. [Q6RVG2-1]
UniGeneiRn.210164.

3D structure databases

ProteinModelPortaliQ6RVG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043825.

PTM databases

iPTMnetiQ6RVG2.
PhosphoSitePlusiQ6RVG2.

Proteomic databases

PaxDbiQ6RVG2.
PRIDEiQ6RVG2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000065379; ENSRNOP00000059220; ENSRNOG00000028879. [Q6RVG2-2]
GeneIDi315311.
KEGGirno:315311.
UCSCiRGD:735164. rat. [Q6RVG2-1]

Organism-specific databases

CTDi9498.
RGDi735164. Slc4a8.

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
GeneTreeiENSGT00760000119021.
HOVERGENiHBG004326.
InParanoidiQ6RVG2.
KOiK13859.
PhylomeDBiQ6RVG2.

Enzyme and pathway databases

ReactomeiR-RNO-425381. Bicarbonate transporters.

Miscellaneous databases

PROiQ6RVG2.

Gene expression databases

ExpressionAtlasiQ6RVG2. differential.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS4A8_RAT
AccessioniPrimary (citable) accession number: Q6RVG2
Secondary accession number(s): Q6RVG1, Q6RVG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.