Q6RSS2 (L_PIRVV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Pirital virus (isolate Rat/Venezuela/VAV-488/1995) (PIRV) [Complete proteome]

Taxonomic identifier

49891 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Sigmodon alstoni [TaxID: 134742]

Protein attributes

Sequence length	2210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Cap snatching RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2210	2210	RNA-directed RNA polymerase L		PRO_0000361645
Regions
	Domain	1163 – 1359	197	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q6RSS2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4552633F41DC58DA	FASTA	2,210	253,470
	10 20 30 40 50 60 MEEHVNELHD LVRKWVSDDE NFAEQKAIFL SQTKLRAITI EGLKLLSTIV EVDSCQKNSC 70 80 90 100 110 120 IHNREKTLNS ILRDNKIVCP TLPEIVPDGY RLIGDVLILL EVFVRSNQES FEKKYEQDYT 130 140 150 160 170 180 KLMQVKKDLQ SHGITLVPMI DGRSSYYVEF MPDWVVEKIR WHLIKLMDLL KEDGESVEEL 190 200 210 220 230 240 EYERLVSSLS ALENQSLGLE SLLSIKERGI EYIDRLTKIM YGNLNNNMSV DECKGEILRI 250 260 270 280 290 300 YQNFRQLFDQ GQFKPKYRKT DREFILKTLR EHGLIKCAIM SEEDSCKNCM IHMFKVLTII 310 320 330 340 350 360 KQSFVSNKNI ESSFILKEYN QLLSVCNKVK SLKVLNTRRG TLMVLDLIML NKLLSLIKIY 370 380 390 400 410 420 GIKAALTILR MQCIPAVNDR LLSIDFLISI YERKMIKSPK WLEKVHGKLK RVVQDCMFKA 430 440 450 460 470 480 LEDYLVEIDF DTWFSIKDEL LMTQQFKPSI CYRSSKGCVC NAETLKNLSM MTEEDFLSYL 490 500 510 520 530 540 KILSSLSLSL VNSMKTSSAP KSKINQANDF YGIVHCEEVY FQGFGDNNAC TLLYQKTGEK 550 560 570 580 590 600 SRCYSVAFSD NEQQIDYGSK ISFYADPKRF FLPIMSQDVL NRMCNEMLSW LDFLSDDNIK 610 620 630 640 650 660 VVADLLRKLI LCVLCTPSKR VQVYLQGFRY LIMAYVNEIH CNDLFAKLEV DALTASERQV 670 680 690 700 710 720 MIWMDDLTRI VLEMSKEADM AKSFKFILNL SYLCHLITKE TPDRLTDQIK CFEKFLEPKL 730 740 750 760 770 780 KFGSLMVNPD STPELTSEQE DQVCEGLHRL LNKKIFSKCE NIPGVSKELV SLCSSLFNSS 790 800 810 820 830 840 NLEVKPLLNH DPLTPSFTST ALDLSSNKSV VVPKLNEIGE TLTEYDFGKI VSSVVVDLTE 850 860 870 880 890 900 HFKTKGKYKL DPRDLRFRIF KKLSSLVEVN PTKKSNRKSE SGEVVAPDES FMDELTEEQQ 910 920 930 940 950 960 LMLSEIEVKV SKTFEGMSKD ELNRKQSKEK GAEAHLKRLW SKEVRDKISS ETSLHEVKDF 970 980 990 1000 1010 1020 DVQLFPFDTY EELVTIVFND KSAHDFYFLE KYLNPCPLDM LMKNLTLKAF NEGDFFECFK 1030 1040 1050 1060 1070 1080 YILIASEFDN KIGRYDHKIR TRLGLKDPAL KIREEARIST RESNSESIAK RLDKSFFTNS 1090 1100 1110 1120 1130 1140 SLRNLCFYSD ESPTTRTGVA TDVGKLKFGL SYKEQVGGNR ELYVGDLNTK LITRLVEDYA 1150 1160 1170 1180 1190 1200 ESICSNMKYT CLNSESEFER ALLDMKSVVR QGGFAVSMDH SKWGPHMSPA IFAQLLRCLK 1210 1220 1230 1240 1250 1260 FRLKDGSEID KKAVLNILYW HLHKIVEVPF NVVQAFMSGF VKRGLGLMDR GGATLSEEFM 1270 1280 1290 1300 1310 1320 FGFFEKGVVP SHLSSVVDMG QGILHNMSDL YGLITEQFIN YVLDFCYNVS MTSYTSSDDE 1330 1340 1350 1360 1370 1380 IMLSTSSALN HEDGSLNVDV ALEILEFHNF LSDKLNKFVS PKTVAGTFAS EFKSRFFIWS 1390 1400 1410 1420 1430 1440 QEVPLLTKFV AAALHNIKAK APNQLAETVD TILDQCVANG VSIEIVGAIS KRTNSLVCYS 1450 1460 1470 1480 1490 1500 GHPLNPFLCL EESDVRDWVD GSRGYRLQRS IENIFPDDLC PNLIRDACRK VFHRIQSGKI 1510 1520 1530 1540 1550 1560 EEEFLVASIQ GSPDECLNSM LTIADVDEDI KKDLAGYRWL NLRAYGDLRL VLRTKLMSST 1570 1580 1590 1600 1610 1620 RTLQREEIPS LVRSVQSKLS KNFVRGAKRI LTDAINKSAF QSCISSGFIG VCKSMGSKCV 1630 1640 1650 1660 1670 1680 RDNTGGFVYI KEITKHVMPH TTSYCPYCKP SKNIYCEDAL RSVSEYSRPI FWDYFSLVLS 1690 1700 1710 1720 1730 1740 NACELGNWVF GAPILPKTVF HLDNPNHFWP IKPSSQTELE DKVGMNHVLY SIRRNYPSIF 1750 1760 1770 1780 1790 1800 DEHISPYMSD LNMLRLSWVQ KIKFLDLCVA LDMSSECLGI ISHIMRRKRE ELYIVKQQEL 1810 1820 1830 1840 1850 1860 SMSHTRESTN LESGLSLEPQ EVCKNFLLQV LFDSMVNPVL LTTSQFRKYF WYGEVLQLPN 1870 1880 1890 1900 1910 1920 DASHHLAQFT QFILDCKQLN SSRAMTLDDL DVGYVTSRVK RTTTFVALST FITSLDWENR 1930 1940 1950 1960 1970 1980 HEYKSFQELI LSSPCDVFKF EFSMTFSHIR SSHKFRYERC TSYILKVHVV FDKRVLNSNM 1990 2000 2010 2020 2030 2040 LEDQSLLITP HSVEYFVSQS GGNHISLDGV GLLPLDPLIS GKEVLNIDDV LRHEDVNFSA 2050 2060 2070 2080 2090 2100 ESPLFSKMRF DFKPFLKELK NKFSYKLIGP DIIMEPLVLD KGQIKEGSRI VSQLKLRLDF 2110 2120 2130 2140 2150 2160 KAVFVALGCL EEESRSTFIS NLFMYIGSLR GEEHRISMTE SNLVQLIDNY PQVFDSMLDA 2170 2180 2190 2200 2210 TNDWLNCGSF SLCKSKSLGC VMIADERGPF KLKGVNCRRL LPDSQAVEID

References

[1]	"Phylogeny of the Venezuelan arenaviruses." Cajimat M.N., Fulhorst C.F. Virus Res. 102:199-206(2004) [PubMed: 15084402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY494081 Genomic RNA. Translation: AAS72554.1.
RefSeq	YP_025093.1. NC_005897.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2943174.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_PIRVV

Accession

Primary (citable) accession number: Q6RSS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 5, 2004
Last modified:	May 31, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program