Q6RJS2 (DHYS_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyhypusine synthase
EC=2.5.1.46

Gene names

Name:

DHS

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine By similarity.
Catalytic activity	[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.
Cofactor	NAD By similarity.
Pathway	Protein modification; eIF5A hypusination.
Sequence similarities	Belongs to the deoxyhypusine synthase family.

Ontologies

Keywords
Biological process	Hypusine biosynthesis
Ligand	NAD
Molecular function	Transferase
Gene Ontology (GO)
Biological_process	deoxyhypusine biosynthetic process from spermidine Inferred from electronic annotation. Source: EC peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	deoxyhypusine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Deoxyhypusine synthase		PRO_0000134474
Regions
	Nucleotide binding	104 – 108	5	NAD By similarity
	Nucleotide binding	130 – 132	3	NAD By similarity
	Nucleotide binding	309 – 310	2	NAD By similarity
	Nucleotide binding	343 – 344	2	NAD By similarity
	Region	135 – 136	2	Spermidine binding By similarity
	Region	315 – 317	3	Spermidine binding By similarity
	Region	324 – 330	7	Spermidine binding By similarity
Sites
	Active site	330	1	Nucleophile By similarity
	Binding site	136	1	NAD By similarity
	Binding site	237	1	NAD By similarity
	Binding site	242	1	Spermidine By similarity
	Binding site	284	1	NAD; via amide nitrogen By similarity
	Binding site	289	1	Spermidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6RJS2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E8B62D427FF6C51F	FASTA	368	41,162
	10 20 30 40 50 60 MEEDRVLSSV HSTVFKESES LEGKCDKIEG YDFNQGVNYP KLLRSMLTTG FQASNLGDVI 70 80 90 100 110 120 DVVNQMLEWR LSDETIAPED CSEEEKDPAY RESVKCKIFL GFTSNLVSSG VRETIRYLVQ 130 140 150 160 170 180 HHMVDVIVTT TGGVEEDLIK CLAPTFKGDF SLPGAYLRSK GLNRIGNLLV PNDNYCKFED 190 200 210 220 230 240 WIIPIFDQML KEQKEESVLW TPSKLLARLG KEINNESSYL YWAYKMNIPV FCRGLTDGSL 250 260 270 280 290 300 GDMLYFHSFR TSGLVIDVVQ DIRAMNGEAV HATPRKTGMI ILGGGLPKHH ICNANMMRNG 310 320 330 340 350 360 ADYAVFINTG QEFDGSDSGA RPDEAVSWGK IRGSAKTVKV YCDATIAFPL LVAETFASKR EQSCEHKT

References

[1]	"Suppression of deoxyhypusine synthase expression in canola using Agrobacterium tumefaciens-mediated transformation." Wang T.-W., Wu W., Zhang C.-G., Thompson J.E. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY496434 mRNA. Translation: AAR91928.1.
3D structure databases
ProteinModelPortal	Q6RJS2.
SMR	Q6RJS2. Positions 27-361.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00354.
Family and domain databases
Gene3D	3.40.910.10. 1 hit.
InterPro	IPR002773. Deoxyhypusine_synthase. [Graphical view]
PANTHER	PTHR11703. PTHR11703. 1 hit.
Pfam	PF01916. DS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00321. dhys. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DHYS_BRANA

Accession

Primary (citable) accession number: Q6RJS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	July 5, 2004
Last modified:	March 6, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program