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Q6RJS2

- DHYS_BRANA

UniProt

Q6RJS2 - DHYS_BRANA

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Protein
Deoxyhypusine synthase
Gene
DHS
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine By similarity.

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

NAD By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361NAD By similarity
Binding sitei237 – 2371NAD By similarity
Binding sitei242 – 2421Spermidine By similarity
Binding sitei284 – 2841NAD; via amide nitrogen By similarity
Binding sitei289 – 2891Spermidine By similarity
Active sitei330 – 3301Nucleophile By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1085NAD By similarity
Nucleotide bindingi130 – 1323NAD By similarity
Nucleotide bindingi309 – 3102NAD By similarity
Nucleotide bindingi343 – 3442NAD By similarity

GO - Molecular functioni

  1. deoxyhypusine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. deoxyhypusine biosynthetic process from spermidine Source: UniProtKB-EC
  2. peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Gene namesi
Name:DHS
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Deoxyhypusine synthase
PRO_0000134474Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6RJS2.
SMRiQ6RJS2. Positions 27-361.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1362Spermidine binding By similarity
Regioni315 – 3173Spermidine binding By similarity
Regioni324 – 3307Spermidine binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6RJS2-1 [UniParc]FASTAAdd to Basket

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MEEDRVLSSV HSTVFKESES LEGKCDKIEG YDFNQGVNYP KLLRSMLTTG    50
FQASNLGDVI DVVNQMLEWR LSDETIAPED CSEEEKDPAY RESVKCKIFL 100
GFTSNLVSSG VRETIRYLVQ HHMVDVIVTT TGGVEEDLIK CLAPTFKGDF 150
SLPGAYLRSK GLNRIGNLLV PNDNYCKFED WIIPIFDQML KEQKEESVLW 200
TPSKLLARLG KEINNESSYL YWAYKMNIPV FCRGLTDGSL GDMLYFHSFR 250
TSGLVIDVVQ DIRAMNGEAV HATPRKTGMI ILGGGLPKHH ICNANMMRNG 300
ADYAVFINTG QEFDGSDSGA RPDEAVSWGK IRGSAKTVKV YCDATIAFPL 350
LVAETFASKR EQSCEHKT 368
Length:368
Mass (Da):41,162
Last modified:July 5, 2004 - v1
Checksum:iE8B62D427FF6C51F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY496434 mRNA. Translation: AAR91928.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY496434 mRNA. Translation: AAR91928.1 .

3D structure databases

ProteinModelPortali Q6RJS2.
SMRi Q6RJS2. Positions 27-361.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00354 .

Family and domain databases

Gene3Di 3.40.910.10. 1 hit.
InterProi IPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view ]
PANTHERi PTHR11703. PTHR11703. 1 hit.
Pfami PF01916. DS. 1 hit.
[Graphical view ]
SUPFAMi SSF52467. SSF52467. 1 hit.
TIGRFAMsi TIGR00321. dhys. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Suppression of deoxyhypusine synthase expression in canola using Agrobacterium tumefaciens-mediated transformation."
    Wang T.-W., Wu W., Zhang C.-G., Thompson J.E.
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDHYS_BRANA
AccessioniPrimary (citable) accession number: Q6RJS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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