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Q6RJS2

- DHYS_BRANA

UniProt

Q6RJS2 - DHYS_BRANA

Protein

Deoxyhypusine synthase

Gene

DHS

Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine By similarity.By similarity

    Catalytic activityi

    [eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

    Cofactori

    NAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei136 – 1361NADBy similarity
    Binding sitei237 – 2371NADBy similarity
    Binding sitei242 – 2421SpermidineBy similarity
    Binding sitei284 – 2841NAD; via amide nitrogenBy similarity
    Binding sitei289 – 2891SpermidineBy similarity
    Active sitei330 – 3301NucleophileBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi104 – 1085NADBy similarity
    Nucleotide bindingi130 – 1323NADBy similarity
    Nucleotide bindingi309 – 3102NADBy similarity
    Nucleotide bindingi343 – 3442NADBy similarity

    GO - Molecular functioni

    1. deoxyhypusine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyhypusine biosynthetic process from spermidine Source: UniProtKB-EC
    2. peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Hypusine biosynthesis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00354.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyhypusine synthase (EC:2.5.1.46)
    Gene namesi
    Name:DHS
    OrganismiBrassica napus (Rape)
    Taxonomic identifieri3708 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 368368Deoxyhypusine synthasePRO_0000134474Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ6RJS2.
    SMRiQ6RJS2. Positions 27-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni135 – 1362Spermidine bindingBy similarity
    Regioni315 – 3173Spermidine bindingBy similarity
    Regioni324 – 3307Spermidine bindingBy similarity

    Sequence similaritiesi

    Belongs to the deoxyhypusine synthase family.Curated

    Family and domain databases

    Gene3Di3.40.910.10. 1 hit.
    InterProiIPR002773. Deoxyhypusine_synthase.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    [Graphical view]
    PANTHERiPTHR11703. PTHR11703. 1 hit.
    PfamiPF01916. DS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52467. SSF52467. 1 hit.
    TIGRFAMsiTIGR00321. dhys. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6RJS2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEDRVLSSV HSTVFKESES LEGKCDKIEG YDFNQGVNYP KLLRSMLTTG    50
    FQASNLGDVI DVVNQMLEWR LSDETIAPED CSEEEKDPAY RESVKCKIFL 100
    GFTSNLVSSG VRETIRYLVQ HHMVDVIVTT TGGVEEDLIK CLAPTFKGDF 150
    SLPGAYLRSK GLNRIGNLLV PNDNYCKFED WIIPIFDQML KEQKEESVLW 200
    TPSKLLARLG KEINNESSYL YWAYKMNIPV FCRGLTDGSL GDMLYFHSFR 250
    TSGLVIDVVQ DIRAMNGEAV HATPRKTGMI ILGGGLPKHH ICNANMMRNG 300
    ADYAVFINTG QEFDGSDSGA RPDEAVSWGK IRGSAKTVKV YCDATIAFPL 350
    LVAETFASKR EQSCEHKT 368
    Length:368
    Mass (Da):41,162
    Last modified:July 5, 2004 - v1
    Checksum:iE8B62D427FF6C51F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY496434 mRNA. Translation: AAR91928.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY496434 mRNA. Translation: AAR91928.1 .

    3D structure databases

    ProteinModelPortali Q6RJS2.
    SMRi Q6RJS2. Positions 27-361.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00354 .

    Family and domain databases

    Gene3Di 3.40.910.10. 1 hit.
    InterProi IPR002773. Deoxyhypusine_synthase.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    [Graphical view ]
    PANTHERi PTHR11703. PTHR11703. 1 hit.
    Pfami PF01916. DS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52467. SSF52467. 1 hit.
    TIGRFAMsi TIGR00321. dhys. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Suppression of deoxyhypusine synthase expression in canola using Agrobacterium tumefaciens-mediated transformation."
      Wang T.-W., Wu W., Zhang C.-G., Thompson J.E.
      Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiDHYS_BRANA
    AccessioniPrimary (citable) accession number: Q6RJS2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3