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Protein

PCNA-associated factor

Gene

Pclaf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-RNO-5656169. Termination of translesion DNA synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
PCNA-associated factorCurated
Alternative name(s):
HCV NS5A-transactivated protein 9 homolog
PCNA-associated factor of 15 kDa
Short name:
PAF15
Short name:
p15PAF
PCNA-clamp-associated factorBy similarity
Gene namesi
Name:PclafBy similarity
Synonyms:Ns5atp9, Paf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1303041. Pclaf.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasmperinuclear region By similarity

  • Note: Following DNA damage, localizes to DNA damage sites. Colocalizes with centrosomes in perinuclear region.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000966861 – 110PCNA-associated factorAdd BLAST110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei24N6-acetyllysine; alternateBy similarity1
Cross-linki24Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei28PhosphoserineBy similarity1
Modified residuei71PhosphoserineBy similarity1

Post-translational modificationi

Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6RIA2.

PTM databases

iPTMnetiQ6RIA2.
PhosphoSitePlusiQ6RIA2.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016561.
GenevisibleiQ6RIA2. RN.

Interactioni

Subunit structurei

Interacts (when monoubiquitinated at Lys-15 and Lys-24) with PCNA. Interacts with isoform 2/p33ING1b of ING1. Interacts with BRCA1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022256.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi23 – 34D-boxAdd BLAST12
Motifi61 – 71PIP-boxAdd BLAST11
Motifi77 – 79KEN box3
Motifi84 – 96Initiation motifAdd BLAST13

Domaini

The PIP-box mediates the interaction with PCNA.By similarity
The KEN box is required for the association with the APC/C complex.By similarity
The D-box (destruction box) mediates the interaction with APC/C proteins, and acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.By similarity
The initiation motif is required for efficient chain initiation by the APC/C complex E2 ligase UBE2C. It determines the rate of substrate's degradation without affecting its affinity for the APC/C, a mechanism used by the APC/C to control the timing of substrate proteolysis during the cell cycle (By similarity).By similarity

Phylogenomic databases

eggNOGiENOG410J101. Eukaryota.
ENOG410Y3KP. LUCA.
GeneTreeiENSGT00510000048252.
HOGENOMiHOG000013069.
HOVERGENiHBG052567.
InParanoidiQ6RIA2.
OMAiDHRDDEN.
OrthoDBiEOG091G13N6.
PhylomeDBiQ6RIA2.
TreeFamiTF333199.

Family and domain databases

InterProiView protein in InterPro
IPR031444. PCNA-AF.
PfamiView protein in Pfam
PF15715. PAF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6RIA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRTKANYVP GAYRKVVASQ APRKVLGSST FVTNSSGSSR KAENKYAGGN
60 70 80 90 100
PVCVRPTPKW QKGIGEFFRL SPKDSKKENQ IPEEAGSSGL GKAKRKACPL
110
QPDHRDDENE
Length:110
Mass (Da):12,006
Last modified:July 5, 2004 - v1
Checksum:i5983F775C66D93B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496944 mRNA. Translation: AAR90859.1.
RefSeqiNP_958821.1. NM_201418.1.
UniGeneiRn.7233.

Genome annotation databases

EnsembliENSRNOT00000022256; ENSRNOP00000022256; ENSRNOG00000016561.
GeneIDi300795.
KEGGirno:300795.
UCSCiRGD:1303041. rat.

Similar proteinsi

Entry informationi

Entry nameiPAF15_RAT
AccessioniPrimary (citable) accession number: Q6RIA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: July 5, 2004
Last modified: August 30, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome